| Budget Amount *help |
¥146,510,000 (Direct Cost: ¥112,700,000、Indirect Cost: ¥33,810,000)
Fiscal Year 2021: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2020: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2019: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2018: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2017: ¥37,310,000 (Direct Cost: ¥28,700,000、Indirect Cost: ¥8,610,000)
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| Outline of Final Research Achievements |
In this study, we carried out with our research the aim of chemically understanding the role of "multi-molecular crowding environment" and developing techniques to control the gene expression. First, cell-mimicked experimental systems in vitro were created by adding macromolecules and metabolites as co-solutes in aqueous solution. Under the cell-mimicked experimental systems, the structure and function of nucleic acids were analyzed using thermodynamic and kinetic analyses. Moreover, we developed database to predict nucleic acid structure and function in cells. Furthermore, using the database, we analyzed changes in the stability and function of the non-canonical DNA and RNA structures. Moreover, we found that the gene expressions were regulated by structure and stability of nucleic acids. Thus, we proposed “Quantitative Function-Environment Relationship (QFER)” of nucleic acids in the cell and developed methods to regulate gene expression based on QFER.
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