| Budget Amount *help |
¥76,180,000 (Direct Cost: ¥58,600,000、Indirect Cost: ¥17,580,000)
Fiscal Year 2021: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2020: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2019: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2018: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2017: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
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| Outline of Final Research Achievements |
Marine sponge Theonella swinhoei with yellow interior (TSY) and T. swinhoei with white interior (TSW) was each dissociated and fractionated to give more than three species of the symbiotic bacteria Entotheonella from each sponge sample, demonstrating the unreported diversity of the Entotheonella. The fractionated Entotheonella was each subjected to cultivation under a variety of conditions in the presence of the "chemical signal" of the host sponge, but the growth of the bacteria was not observed. On the other hand, the microbiota prepared from a marine sponge Mycale sp. was subjected to the metagenomic analysis, which gave the whole genome data of five symbiotic bacteria, permitting us to identify the bacteria which contained the biosynthetic gene cluster of mycalolides.
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