Development of analysis technology for chemical signal based on proteomics

• Project Area: Frontier research of chemical communications
• Project/Area Number: 17H06412
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Osada Hiroyuki 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (80160836)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥76,180,000 (Direct Cost: ¥58,600,000、Indirect Cost: ¥17,580,000); Fiscal Year 2021: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2020: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2019: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2018: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2017: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
• Keywords: プロテオミクス / 生理活性物質 / 標的同定 / プロテオーム / 阻害剤 / 生物活性リガンド / proteome analysis / chemicalbiology / drug target

Outline of Final Research Achievements

To improve the drug target analysis system, ChemProteoBase, we obtained proteomic data of HeLa cells treated with a variety of well-known inhibitors to expand the database and developed new tools on the ChemProteoBase system for biological pathway analysis and keyword analysis. We also developed a new direct drug target identification method, 2DE-CETSA, which combines 2D electrophoresis and cellular thermal shift assay, utilizing the ChemProteoBase system and the database. Screening of bioactive ligands targeting cancer cell metabolism was performed, and NPD10084 was found to be targeted to PKM2. In addition, we found other novel compounds affecting cancer metabolisms and identified their targets. Furthermore, a number of novel biological active compounds from natural products were identified.

Academic Significance and Societal Importance of the Research Achievements

有用な生物活性リガンドが利用されるためには、その標的分子が明らかであることが必要である。これらには表現型スクリーニングで得られた化合物も多く、酵素阻害剤などのように目的とする分子が想定された化合物とは異なり、標的分子を同定することは必ずしも容易ではない。薬剤標的を直接法（2DE-CETSA）と間接法（ChemProteoBase）の双方を用いてプロテオームワイドに解析できる独自のケモプロテオミクス基盤は、生物活性リガンドの標的同定を加速させるとともに、本プロジェクトで見出された新規生物活性物質は、今後バイオプローブや医薬品等の開発に貢献することが期待される。

Research Products

• [Int'l Joint Research] マックス・プランク研究所/ザールランド大学(ドイツ)
• [Int'l Joint Research] 韓国生命工学研院(KRIBB)(韓国)
• [Int'l Joint Research] 浙江大学/曁南大学(中国)
• [Int'l Joint Research] マレーシア科学大学(USM)(マレーシア)
• [Int'l Joint Research] マックス・プランク研究所/ザールランド大学(ドイツ)
• [Int'l Joint Research] 韓国生命工学研院(KRIBB)(韓国)
• [Int'l Joint Research] 浙江大学/曁南大学(中国)
• [Int'l Joint Research] マレーシア科学大学(USM)(マレーシア)
• [Int'l Joint Research] マックス・プランク研究所/ザールランド大学(ドイツ)
• [Int'l Joint Research] 韓国生命工学研院(KRIBB)(韓国)
• [Int'l Joint Research] マレーシア科学大学(USM)(マレーシア)
• [Int'l Joint Research] 浙江大学/東北師範大学/曁南大学(中国)
• [Int'l Joint Research] マックス・プランク研究所/ザールランド大学(ドイツ)
• [Int'l Joint Research] 韓国生命工学研院(KRIBB)(韓国)
• [Int'l Joint Research] マレーシア科学大学(USM)(マレーシア)
• [Int'l Joint Research] 浙江大学(中国)
• [Journal Article] Isoquercitrin from <i>Apocynum venetum</i> L. produces an anti-obesity effect on obese mice by targeting C-1-tetrahydrofolate synthase, carbonyl reductase, and glutathione S-transferase P and modification of the AMPK/SREBP-1c/FAS/CD36 signaling pathway in mice <i>in vivo</i>. (2022)
  • Author(s): Manzoor M, Muroi M, Ogawa N, Kobayashi H, Nishimura H, Chen D, Fasina OB, Wang J, Osada H, Yoshida M, Xiang L, Qi J
  • Journal Title: Food Funct Volume: 13 Issue: 21 Pages: 10923-10936
  • DOI: 10.1039/d2fo02438a
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two-dimensional electrophoresis-cellular thermal shift assay (2DE-CETSA) for target identification of bioactive compounds. (2022)
  • Author(s): Muroi M, Osada H
  • Journal Title: Methods Enzymol Volume: 675 Pages: 425-437
  • DOI: 10.1016/bs.mie.2022.07.018
  • ISBN: 9780323992664
  • Peer Reviewed
• [Journal Article] Identification of a small molecule that enhances ferroptosis via inhibition of FSP1. (2022)
  • Author(s): Yoshioka H, Kawamura T, Muroi M, Kondoh Y, Honda K, Kawatani M, Aono H, Waldmann H, Watanabe N, Osada H.
  • Journal Title: ACS Chemical Biology Volume: 17 Issue: 2 Pages: 483-491
  • DOI: 10.1021/acschembio.2c00028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] RK-270D and E, Oxindole Derivatives from Streptomyces sp. with Anti-Angiogenic Activity (2022)
  • Author(s): Jang Jun-Pil、Jang Mina、Nogawa Toshihiko、Takahashi Shunji、Osada Hiroyuki、Ahn Jong Seog、Ko Sung-Kyun、Jang Jae-Hyuk
  • Journal Title: Journal of Microbiology and Biotechnology Volume: 32 Issue: 3 Pages: 302-306
  • DOI: 10.4014/jmb.2110.10039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] β-Methyltryptamine Provoking the Crucial Role of Strictosidine Synthase Tyr151-OH for Its Stereoselective Pictet-Spengler Reactions to Tryptoline-type Alkaloids (2022)
  • Author(s): Liu Haicheng、Panjikar Santosh、Sheng Xiang、Futamura Yushi、Zhang Chenghua、Shao Nana、Osada Hiroyuki、Zou Hongbin
  • Journal Title: ACS Chemical Biology Volume: 17 Issue: 1 Pages: 187-197
  • DOI: 10.1021/acschembio.1c00844
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Establishment of a Monoclonal Antibody against Human NTCP That Blocks Hepatitis B Virus Infection (2022)
  • Author(s): Takemori Toshitada、Sugimoto-Ishige Akiko、Nishitsuji Hironori、Futamura Yushi、Harada Michishige、Kimura-Someya Tomomi、et al.
  • Journal Title: Journal of Virology Volume: 96 Issue: 5
  • DOI: 10.1128/jvi.01686-21
  • Peer Reviewed
• [Journal Article] Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite (2022)
  • Author(s): Mendoza, A., Takemoto, Y., Cruzado, KT., Masoud, SS., Nagata, A., Tantipanjaporn, A., Okuda, S., Kawagoe, F., Sakamoto, R., Odagi, M., Mototani, S., Togashi, M., Kawatani, M., Aono, H., Osada, H., Nakagawa, H., Higashi, T., Kittaka, A., Nagasawa, K., Uesugi, M.
  • Journal Title: Cell Chem Biol. Volume: 29 Issue: 4 Pages: 660-669
  • DOI: 10.1016/j.chembiol.2021.08.008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment (2021)
  • Author(s): J. Yoshida, T. Ohishi, H. Abe, S. Ohba, H. Inoue, I. Usami, M. Amemiya, R. Oriez, C. Sakashita, S. Dan, M. Sugawara, T. Kawaguchi, J. Ueno, Y. Asano, A. Ikeda, M. Takamatsu, G. Amori, Y. Kondoh, K. Honda, H. Osada, T. Noda, T. Watanabe, T. Shimizu, M. Shibasaki and M. Kawada
  • Journal Title: iScience Volume: 24 Issue: 12 Pages: 103497-103497
  • DOI: 10.1016/j.isci.2021.103497
  • Peer Reviewed / Open Access
• [Journal Article] Dihydrolucilactaene, a Potent Antimalarial Compound from Fusarium sp. RK97-94 (2021)
  • Author(s): A. Abdelhakim Islam、Bin Mahmud Fauze、Motoyama Takayuki、Futamura Yushi、Takahashi Shunji、Osada Hiroyuki
  • Journal Title: Journal of Natural Products Volume: 85 Issue: 1 Pages: 63-69
  • DOI: 10.1021/acs.jnatprod.1c00677
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae (2021)
  • Author(s): Lee Jee Whu、Ong Tee Gee、Samian Mohammed Razip、Teh Aik-Hong、Watanabe Nobumoto、Osada Hiroyuki、Ong Eugene Boon Beng
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 24148-24148
  • DOI: 10.1038/s41598-021-03490-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oxicam-type non-steroidal anti-inflammatory drugs inhibit NPR1-mediated salicylic acid pathway (2021)
  • Author(s): Ishihama Nobuaki、Choi Seung-won、Noutoshi Yoshiteru、Saska Ivana、Asai Shuta、Takizawa Kaori、He Sheng Yang、Osada Hiroyuki、Shirasu Ken
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 7303-7303
  • DOI: 10.1038/s41467-021-27489-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tenuazonic acid production is dispensable for virulence, but its biosynthetic gene expression pattern is associated with the infection of Pyricularia oryzae (2021)
  • Author(s): Kashiwa Takeshi、Motoyama Takayuki、Yoshida Kazuko、Yun Choong-Soo、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 86 Issue: 1 Pages: 135-139
  • DOI: 10.1093/bbb/zbab195
  • Peer Reviewed
• [Journal Article] 2-Methylthio-N7-methyl-cis-zeatin, a new antimalarial natural product isolated from a Streptomyces culture (2021)
  • Author(s): Lopez Julius Adam V、Nogawa Toshihiko、Yoshida Kazuko、Futamura Yushi、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 86 Issue: 1 Pages: 31-36
  • DOI: 10.1093/bbb/zbab192
  • Peer Reviewed
• [Journal Article] Identification of Dihydroorotate Dehydrogenase Inhibitors─Indoluidins─That Inhibit Cancer Cell Growth (2021)
  • Author(s): Kawatani Makoto、Aono Harumi、Shimizu Takeshi、Ohkura Shouta、Hiranuma Sayoko、Muroi Makoto、Ogawa Naoko、Ohishi Tomokazu、Ohba Shun-ichi、Kawada Manabu、Yamazaki Kanami、Dan Shingo、Osada Hiroyuki
  • Journal Title: ACS Chemical Biology Volume: 16 Issue: 11 Pages: 2570-2580
  • DOI: 10.1021/acschembio.1c00625
  • Peer Reviewed
• [Journal Article] Privileged Biorenewable Secologanin‐Based Diversity‐Oriented Synthesis for Pseudo‐Natural Alkaloids: Uncovering Novel Neuroprotective and Antimalarial Frameworks (2021)
  • Author(s): Zhu Huajian、Cai Yunrui、Ma Shijia、Futamura Yushi、Li Jinbiao、Zhong Wen、Zhang Xiangnan、Osada Hiroyuki、Zou Hongbin
  • Journal Title: ChemSusChem Volume: 14 Issue: 23 Pages: 5320-5327
  • DOI: 10.1002/cssc.202101868
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Thiolactomide: A New Homocysteine Thiolactone Derivative from Streptomyces sp. with Neuroprotective Activity (2021)
  • Author(s): Jang Jun-Pil、Kwon Min Cheol、Nogawa Toshihiko、Takahashi Shunji、Osada Hiroyuki、Ahn Jong Seog、Ko Sung-Kyun、Jang Jae-Hyuk
  • Journal Title: Journal of Microbiology and Biotechnology Volume: 31 Issue: 12 Pages: 1667-1671
  • DOI: 10.4014/jmb.2108.08015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Does local injection of reveromycin A inhibit tooth movement without causing systemic side effects? (2021)
  • Author(s): Kako Shunsuke、Tabuchi Masako、Miyazawa Ken、Tanaka Miyuki、Minamoto Chisato、Asano Yuichiro、Kimura Fumika、Aoki Yuki、Sato Takuma、Kawatani Makoto、Osada Hiroyuki、Maeda Hatsuhiko、Goto Shigemi
  • Journal Title: European Journal of Orthodontics Volume: - Issue: 6 Pages: 658-664
  • DOI: 10.1093/ejo/cjaa067
  • Peer Reviewed / Open Access
• [Journal Article] Alantolactone is a natural product that potently inhibits YAP1/TAZ through promotion of reactive oxygen species accumulation. (2021)
  • Author(s): Nakatani K, Maehama T, Nishio M, Otani J, Yamaguchi K, Fukumoto M, Hikasa H, Hagiwara S, Nishina H, Mak TW, Honma T, Kondoh Y, Osada H, Yoshida M, Suzuki A
  • Journal Title: Cancer Science Volume: 112 Issue: 10 Pages: 4303-4316
  • DOI: 10.1111/cas.15079
  • NAID: 120007159906
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A cesium tolerance-enhancing chemical promotes cesium tolerance in Arabidopsis thaliana by interacting with BETA-GLUCOSIDASE 23. (2021)
  • Author(s): Moon JY, Adams E, Miyazaki T, Kondoh Y, Muroi M, Watanabe N, Osada H, Shin R
  • Journal Title: Scientific Reports Volume: 26 Issue: 1 Pages: 21109-21109
  • DOI: 10.1038/s41598-021-00564-4
  • Peer Reviewed / Open Access
• [Journal Article] Pharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock (2021)
  • Author(s): Takeharu Sakamoto, Yuya Fukui, Yasumitsu Kondoh, Kaori Honda, Takeshi Shimizu, Toshiro Hara, Tetsuro Hayashi, Yurika Saitoh, Yoshinori Murakami, Jun-Ichiro Inoue, Shuichi Kaneko, Hiroyuki Osada, Motoharu Seiki
  • Journal Title: Communications Biology Volume: 4 Issue: 1 Pages: 1165-1165
  • DOI: 10.1038/s42003-021-02701-1
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Basis for Two Stereoselective Diels-Alderases that Produce Decalin Skeletons (2021)
  • Author(s): Fujiyama Keisuke、Kato Naoki、Re Suyong、Kinugasa Kiyomi、Watanabe Kohei、Takita Ryo、Nogawa Toshihiko、Hino Tomoya、Osada Hiroyuki、Sugita Yuji、Takahashi Shunji、Nagano Shingo
  • Journal Title: Angewandte Chemie International Edition Volume: 60 Issue: 41 Pages: 22401-22410
  • DOI: 10.1002/anie.202106186
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Screening of tenuazonic acid production-inducing compounds and identification of NPD938 as a regulator of fungal secondary metabolism (2021)
  • Author(s): Motoyama Takayuki、Ishii Tomoaki、Kamakura Takashi、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 10 Pages: 2200-2208
  • DOI: 10.1093/bbb/zbab143
  • Peer Reviewed
• [Journal Article] Local administration of ReveromycinA ointment suppressed alveolar bone loss in mice (2021)
  • Author(s): Miyazawa Ken、Asano Yuichiro、Tabuchi Masako、Kako Shunsuke、Kawatani Makoto、Osada Hiroyuki、Maeda Hatsuhiko、Goto Shigemi
  • Journal Title: Journal of Pharmacological Sciences Volume: 147 Issue: 1 Pages: 40-47
  • DOI: 10.1016/j.jphs.2021.05.011
  • Peer Reviewed
• [Journal Article] 6,9-Dihydroxytetrangulol, a novel angucyclinone antibiotic accumulated in kiqO gene disruptant in the biosynthesis of kinanthraquinone (2021)
  • Author(s): Sakai Katsuyuki、Takao Risa、Koshino Hiroyuki、Futamura Yushi、Osada Hiroyuki、Takahashi Shunji
  • Journal Title: The Journal of Antibiotics Volume: 74 Issue: 9 Pages: 593-595
  • DOI: 10.1038/s41429-021-00442-1
  • Peer Reviewed
• [Journal Article] Improving measures of chemical structural similarity using machine learning on chemical-genetic interactions (2021)
  • Author(s): Safizadeh, H., Simpkins, SW., Nelson, J., Li, SC., Piotrowski, JS., Yoshimura, M., Yashiroda, Y., Hirano, H., Osada, H., Yoshida, M., Boone, C., Myers, CL.
  • Journal Title: Journal of Chemical Information and Modeling Volume: 61 Issue: 9 Pages: 4156-4172
  • DOI: 10.1021/acs.jcim.0c00993
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Zealpeptaibolin, an 11-mer cytotoxic peptaibol group with 3 Aib-Pro motifs isolated from Trichoderma sp. RK10-F026 (2021)
  • Author(s): Rawa Mira Syahfriena Amir、Nogawa Toshihiko、Okano Akiko、Futamura Yushi、Wahab Habibah A.、Osada Hiroyuki
  • Journal Title: The Journal of Antibiotics Volume: 74 Issue: 8 Pages: 485-495
  • DOI: 10.1038/s41429-021-00429-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of a Small-Molecule Glucose Transporter Inhibitor, Glutipyran, That Inhibits Cancer Cell Growth (2021)
  • Author(s): Kawatani Makoto、Aono Harumi、Hiranuma Sayoko、Shimizu Takeshi、Muroi Makoto、Ogawa Naoko、Ohishi Tomokazu、Ohba Shun-ichi、Kawada Manabu、Nogawa Toshihiko、Okano Akiko、Hashizume Daisuke、Osada Hiroyuki
  • Journal Title: ACS Chemical Biology Volume: 16 Issue: 8 Pages: 1576-1586
  • DOI: 10.1021/acschembio.1c00480
  • Peer Reviewed
• [Journal Article] New abscisic acid derivatives revealed adequate regulation of stomatal, transcriptional, and developmental responses to conquer drought. (2021)
  • Author(s): Yoshida K, Kondoh Y, Nakano T, Bolortuya B, Kawabata S, Iwahashi F, Nagano E, Osada H
  • Journal Title: ACS Chemical Biology Volume: 16 Issue: 8 Pages: 1566-1575
  • DOI: 10.1021/acschembio.1c00451
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] CSE1L promotes nuclear accumulation of transcriptional coactivator TAZ and enhances invasiveness of human cancer cells. (2021)
  • Author(s): Nagashima S, Maruyama J, Honda K, Kondoh Y, Osada H, Nawa M, Nakahama KI, Ishigami-Yuasa M, Kagechika H, Sugimura H, Iwasa H, Arimoto-Matsuzaki K, Nishina H, Hata Y
  • Journal Title: Journal of Biological Chemistry Volume: 297 Issue: 1 Pages: 100803-100803
  • DOI: 10.1016/j.jbc.2021.100803
  • Peer Reviewed / Open Access
• [Journal Article] N-Acetyl-α-hydroxy-β-oxotryptamine, a racemic natural product isolated from Streptomyces sp. 80H647 (2021)
  • Author(s): Lopez Julius Adam V.、Nogawa Toshihiko、Futamura Yushi、Aono Harumi、Hashizume Daisuke、Osada Hiroyuki
  • Journal Title: The Journal of Antibiotics Volume: ahead of online Issue: 7 Pages: 477-479
  • DOI: 10.1038/s41429-021-00420-7
  • Peer Reviewed
• [Journal Article] Targeting Epigenetic and Posttranscriptional Gene Regulation by PSF Impairs Hormone Therapy-Refractory Cancer Growth (2021)
  • Author(s): Takayama Ken-ichi、Honma Teruki、Suzuki Takashi、Kondoh Yasumitsu、Osada Hiroyuki、Suzuki Yutaka、Yoshida Minoru、Inoue Satoshi
  • Journal Title: Cancer Research Volume: 81 Issue: 13 Pages: 3495-3508
  • DOI: 10.1158/0008-5472.can-20-3819
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of a σ1 receptor antagonist by combination of unbiased cell painting and thermal proteome profiling. (2021)
  • Author(s): Wilke J, Kawamura T, Xu H, Brause A, Friese A, Metz M, Schepmann D, Wunsch B, Artacho-Cordon A, Nieto FR, Watanabe N, Osada H, Ziegler S, Waldmann H.
  • Journal Title: Cell Chem Biol Volume: - Issue: 6 Pages: 1-7
  • DOI: 10.1016/j.chembiol.2021.01.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Insulin Receptor: A Potential Target of Amarogentin Isolated from Gentiana rigescens Franch That Induces Neurogenesis in PC12 Cells (2021)
  • Author(s): Lihong Cheng, Hiroyuki Osada, Tianyan Xing, Minoru Yoshida, Lan Xiang and Jianhua Qi
  • Journal Title: BIOMEDICINES Volume: 9 Issue: 5 Pages: 581-581
  • DOI: 10.3390/biomedicines9050581
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glyoxalase I disruption and external carbonyl stress impair mitochondrial function in human induced pluripotent stem cells and derived neurons (2021)
  • Author(s): Hara Tomonori、Toyoshima Manabu、Hisano Yasuko、Balan Shabeesh、Iwayama Yoshimi、Aono Harumi、Futamura Yushi、Osada Hiroyuki、Owada Yuji、Yoshikawa Takeo
  • Journal Title: Translational Psychiatry Volume: 11 Issue: 1
  • DOI: 10.1038/s41398-021-01392-w
  • Peer Reviewed
• [Journal Article] Dihydropyriculol produced by Pyricularia oryzae inhibits the growth of Streptomyces griseus (2021)
  • Author(s): Furuyama Yuuki、Motoyama Takayuki、Nogawa Toshihiko、Kamakura Takashi、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 5 Pages: 1290-1293
  • DOI: 10.1093/bbb/zbab021
  • Peer Reviewed / Open Access
• [Journal Article] Introduction and commentaries for the special issue: “Arnold L. Demain-A life lived” (2021)
  • Author(s): Baltz Richard H、Vandamme Erick J、Bennett Joan W、Agathos Spiros N、Sanchez Sergio、Osada Hiroyuki、Deng Zixin、Gonzalez Ramon
  • Journal Title: Journal of Industrial Microbiology and Biotechnology Volume: 48 Issue: 9-10
  • DOI: 10.1093/jimb/kuab082
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Biosynthesis and biological function of secondary metabolites of the rice blast fungus Pyricularia oryzae (2021)
  • Author(s): Motoyama Takayuki、Yun Choong-Soo、Osada Hiroyuki
  • Journal Title: Journal of Industrial Microbiology and Biotechnology Volume: 48 Issue: 9-10
  • DOI: 10.1093/jimb/kuab058
  • Peer Reviewed / Open Access
• [Journal Article] Proteomics-based target identification of natural products affecting cancer metabolism. (2021)
  • Author(s): Muroi M, Osada H.
  • Journal Title: J Antibiot Volume: 74 Issue: 10 Pages: 639-650
  • DOI: 10.1038/s41429-021-00437-y
  • Peer Reviewed
• [Journal Article] Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator. (2021)
  • Author(s): Takao R, Sakai K, Koshino H, Osada H, Takahashi S.
  • Journal Title: Biosci Biotechnol Biochem Volume: 85 Issue: 3 Pages: 714-721
  • DOI: 10.1093/bbb/zbaa082
  • Peer Reviewed
• [Journal Article] Controlling the production of phytotoxin pyriculol in Pyricularia oryzae by aldehyde reductase (2021)
  • Author(s): Furuyama Yuuki、Motoyama Takayuki、Nogawa Toshihiko、Hayashi Toshiaki、Hirota Hiroshi、Kiyota Hiromasa、Kamakura Takashi、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 1 Pages: 126-133
  • DOI: 10.1093/bbb/zbaa035
  • Peer Reviewed / Open Access
• [Journal Article] A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening (2021)
  • Author(s): Rawa Mira Syahfriena Amir、Nogawa Toshihiko、Okano Akiko、Futamura Yushi、Nakamura Takemichi、Wahab Habibah A、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 1 Pages: 69-76
  • DOI: 10.1093/bbb/zbaa051
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dutomycin induces autophagy and apoptosis by targeting the serine protease Inhibitor SERPINB6. (2021)
  • Author(s): Jang M, Hara S, Kim GH, Kim SM, Son S, Kwon M, Ryoo IJ, Seo H, Kim MJ, Kim ND, Soung NK, Kwon YT, Kim BY, Osada H, Jang JH, Ko SK, Ahn JS.
  • Journal Title: ACS Chem Biol Volume: 16 Issue: 2 Pages: 360-370
  • DOI: 10.1021/acschembio.0c00889
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chemical reversal of abnormalities in cells carrying mitochondrial DNA mutations. (2021)
  • Author(s): Kobayashi H, Hatakeyama H, Nishimura H, Yokota M, Suzuki S, Tomabechi Y, Shirouzu M, Osada H, Mimaki M, Goto YI, Yoshida M.
  • Journal Title: Nat Chem Biol Volume: 17 Issue: 3 Pages: 335-343
  • DOI: 10.1038/s41589-020-00676-4
  • Peer Reviewed
• [Journal Article] Discovery of 3-cinnamamido-N-substituted benzamides as potential antimalarial agents. (2020)
  • Author(s): Liu H, Futamura Y, Wu H, Ishiyama A, Zhang T, Shi T, Zheng Q, Iwatsuki M, Omura S, Zou H, Osada H.
  • Journal Title: Med Chem Volume: - Issue: 10 Pages: 1207-1218
  • DOI: 10.2174/1573406416666200817160708
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reveromycin A, a novel acid-seeking agent, ameliorates bone destruction and tumor growth in multiple myeloma. (2020)
  • Author(s): Keiichiro Watanabe、Ariunzaya Bat-Erdene、Hirofumi Tenshin、Qu Cui、Jumpei Teramachi、Masahiro Hiasa、Asuka Oda、Takeshi Harada、Hirokazu Miki、Kimiko Sogabe、Masahiro Oura、Ryohei Sumitani、Yukari Mitsui、Itsuro Endo、Eiji Tanaka、Makoto Kawatani、Hiroyuki Osada、Toshio Matsumoto、Masahiro Abe
  • Journal Title: Haematologica Volume: 106 Issue: 4 Pages: 1172-1177
  • DOI: 10.3324/haematol.2019.244418
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a selective RelA inhibitor based on DSE-FRET screening methods. (2020)
  • Author(s): Shiroma Y, Fujita G, Yamamoto T, Takahashi RU, Kumar A, Zhang KYJ, Ito A, Osada H, Yoshida M, Tahara H.
  • Journal Title: Int J Mol Sci Volume: 21 Issue: 23 Pages: 9150-9150
  • DOI: 10.3390/ijms21239150
  • Peer Reviewed / Open Access
• [Journal Article] Heterologous Expression of the Biosynthetic Gene Cluster for Verticilactam and Identification of Analogues (2020)
  • Author(s): Nogawa Toshihiko、Terai Atsutaka、Amagai Keita、Hashimoto Junko、Futamura Yushi、Okano Akiko、Fujie Manabu、Satoh Noriyuki、Ikeda Haruo、Shin-ya Kazuo、Osada Hiroyuki、Takahashi Shunji
  • Journal Title: Journal of Natural Products Volume: 83 Issue: 12 Pages: 3598-3605
  • DOI: 10.1021/acs.jnatprod.0c00755
  • Peer Reviewed
• [Journal Article] Pesticide treatment reduces hydrophobic pollutant contamination in Cucurbita pepo through competitive binding to major latex-like proteins. (2020)
  • Author(s): Fujita K, Kondoh Y, Honda K, Haga Y, Osada H, Matsumura C, Inui H.
  • Journal Title: Environ Pollut Volume: 266 Pages: 115179-115179
  • DOI: 10.1016/j.envpol.2020.115179
  • Peer Reviewed
• [Journal Article] Interaction between goniothalamin and peroxisomal multifunctional enzyme type 2 triggering endoplasmic reticulum stress (2020)
  • Author(s): Sophonnithiparasert T, Aruksakunwong O, Tashiro E, Kondoh Y, Muroi M, Osada H, Imoto M, Watanapokasin R
  • Journal Title: Heliyon Volume: 6 Issue: 10 Pages: e05200-e05200
  • DOI: 10.1016/j.heliyon.2020.e05200
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Discovery of small-molecule modulator of heterotrimeric Gi-protein by integrated phenotypic profiling and chemical proteomics (2020)
  • Author(s): 16)Kawamura, T., Futamura, Y., Shang, E., Muroi, M., Janning, P., Ueno, M., Wilke, J., Takeda, S., Kondoh, Y., Ziegler, S., Watanabe, N., Waldmann, H., Osada, H.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 84 Issue: 12 Pages: 2484-2490
  • DOI: 10.1080/09168451.2020.1812375
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel GSK3 inhibitor that promotes self-renewal in mouse embryonic stem cells. (2020)
  • Author(s): Kobayashi H, Nishimura H, Kudo N, Osada H, Yoshida M.
  • Journal Title: Biosci Biotechnol Biochem Volume: 84 Issue: 10 Pages: 2113-2120
  • DOI: 10.1080/09168451.2020.1789445
  • Peer Reviewed / Open Access
• [Journal Article] Uncovering hidden sesquiterpene biosynthetic pathway through expression boost area-mediated productivity enhancement in basidiomycete (2020)
  • Author(s): Asai Shihori、Tsunematsu Yuta、Masuya Takahiro、Otaka Junnosuke、Osada Hiroyuki、Watanabe Kenji
  • Journal Title: The Journal of Antibiotics Volume: 73 Issue: 10 Pages: 721-728
  • DOI: 10.1038/s41429-020-0355-9
  • Peer Reviewed
• [Journal Article] Peanut skin extract ameliorates the symptoms of type 2 diabetes mellitus in mice by alleviating inflammation and maintaining gut microbiota homeostasis. (2020)
  • Author(s): Xiang L, Wu Q, Osada H, Yoshida M, Pan W, Qi J
  • Journal Title: Aging Volume: 12 Issue: 14 Pages: 13991-14018
  • DOI: 10.18632/aging.103521
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Miclxin, a novel MIC60 inhibitor, induces apoptosis via mitochondrial stress in β-catenin mutant tumor cells. (2020)
  • Author(s): 20)Ikeda, H., Muroi, M., Kondoh, Y., Ishikawa, S., Kakeya, H., Osada, H., Imoto, M.
  • Journal Title: ACS Chem. Biol. Volume: 15 Issue: 8 Pages: 2195-2204
  • DOI: 10.1021/acschembio.0c00381
  • Peer Reviewed / Open Access
• [Journal Article] Unique features of the ketosynthase domain in a nonribosomal peptide synthetase?polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1 (2020)
  • Author(s): Yun Choong-Soo、Nishimoto Kazuki、Motoyama Takayuki、Shimizu Takeshi、Hino Tomoya、Dohmae Naoshi、Nagano Shingo、Osada Hiroyuki
  • Journal Title: Journal of Biological Chemistry Volume: 295 Issue: 33 Pages: 11602-11612
  • DOI: 10.1074/jbc.ra120.013105
  • Peer Reviewed / Open Access
• [Journal Article] β-carboline chemical signals induce reveromycin production through a LuxR family regulator in Streptomyces sp. SN-593. (2020)
  • Author(s): Panthee S, Kito N, Hayashi T, Shimizu T, Ishikawa J, Hamamoto H, Osada H, Takahashi S.
  • Journal Title: Sci Rep Volume: 10 Issue: 1 Pages: 10230-10230
  • DOI: 10.1038/s41598-020-66974-y
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana (2020)
  • Author(s): Sako Kaori、Futamura Yushi、Shimizu Takeshi、Matsui Akihiro、Hirano Hiroyuki、Kondoh Yasumitsu、Muroi Makoto、Aono Harumi、Tanaka Maho、Honda Kaori、Shimizu Kenshirou、Kawatani Makoto、Nakano Takeshi、Osada Hiroyuki、Noguchi Ko、Seki Motoaki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 8691-8691
  • DOI: 10.1038/s41598-020-65614-9
  • NAID: 120006876594
  • Peer Reviewed / Open Access
• [Journal Article] Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene (2020)
  • Author(s): Kato Sho、Motoyama Takayuki、Uramoto Masakazu、Nogawa Toshihiko、Kamakura Takashi、Osada Hiroyuki
  • Journal Title: The Journal of Antibiotics Volume: － Issue: 7 Pages: 475-479
  • DOI: 10.1038/s41429-020-0295-4
  • Peer Reviewed / Open Access
• [Journal Article] Biosynthetic gene cluster identification and biological activity of lucilactaene from Fusarium sp. RK97-94 (2020)
  • Author(s): Kato Sho、Motoyama Takayuki、Futamura Yushi、Uramoto Masakazu、Nogawa Toshihiko、Hayashi Toshiaki、Hirota Hiroshi、Tanaka Akira、Takahashi-Ando Naoko、Kamakura Takashi、Osada Hiroyuki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 84 Issue: 6 Pages: 1303-1307
  • DOI: 10.1080/09168451.2020.1725419
  • Peer Reviewed
• [Journal Article] Productivity and bioactivity of enokipodins A-D of Flammulina rossica and Flammulina velutipes (2020)
  • Author(s): Tabuchi Akiko、Fukushima-Sakuno Emi、Osaki-Oka Kumiko、Futamura Yushi、Motoyama Takayuki、Osada Hiroyuki、Ishikawa Noemia Kazue、Nagasawa Eiji、Tokimoto Keisuke
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 84 Issue: 5 Pages: 876-886
  • DOI: 10.1080/09168451.2020.1714421
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] CRM646-A, a fungal metabolite, induces nucleus condensation by increasing Ca2+ levels in rat 3Y1 fibroblast cells. (2020)
  • Author(s): Asami Y, Kim SO, Jang JP, Ko SK, Kim BY, Osada H, Jang JH, Ahn JS.
  • Journal Title: J Microbiol Biotechnol Volume: 30 Issue: 1 Pages: 31-37
  • DOI: 10.4014/jmb.1908.08043
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Measurement of ATPase Activity of Valosin-containing Protein/p97 (2020)
  • Author(s): Suvarna Kruthi、Honda Kaori、Muroi Makoto、Kondoh Yasumitsu、Osada Hiroyuki、Watanabe Nobumoto
  • Journal Title: BIO-PROTOCOL Volume: 10 Issue: 3
  • DOI: 10.21769/bioprotoc.3516
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry (2020)
  • Author(s): Suvarna Kruthi、Honda Kaori、Muroi Makoto、Kondoh Yasumitsu、Watanabe Nobumoto、Osada Hiroyuki
  • Journal Title: BIO-PROTOCOL Volume: 10 Issue: 3
  • DOI: 10.21769/bioprotoc.3517
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a Small Compound Targeting PKM2-Regulated Signaling Using 2D Gel Electrophoresis-Based Proteome-wide CETSA (2020)
  • Author(s): Nagasawa Ikuko、Muroi Makoto、Kawatani Makoto、Ohishi Tomokazu、Ohba Shun-ichi、Kawada Manabu、Osada Hiroyuki
  • Journal Title: Cell Chemical Biology Volume: 27 Issue: 2 Pages: 1-11
  • DOI: 10.1016/j.chembiol.2019.11.010
  • Peer Reviewed
• [Journal Article] Alteration of tooth movement by reveromycin A in OPG KO mice (2020)
  • Author(s): Minamoto C., Miyazawa K., Tabuchi M., Tanaka H., Mizuno M., Yoshizako M., Torii Y., Tamaoka Y., Asano Y., Kawatani M., Osada H., Maeda H., and Goto S.
  • Journal Title: Am. J. Orthod. Dentofacial Orthop. Volume: 157 Issue: 5 Pages: 680-689
  • DOI: 10.1016/j.ajodo.2019.04.037
  • Peer Reviewed / Open Access
• [Journal Article] ゲノム創薬の潮流 (2020)
  • Author(s): 長田 裕之
  • Journal Title: 腫瘍内科 Volume: 25 Pages: 2-8
• [Journal Article] An integrated screening system for the selection of exemplary substrates for natural and engineered cytochrome P450s (2019)
  • Author(s): Kanoh Naoki, Kawamata-Asano Ayano, Suzuki Kana, Takahashi Yusuke, Miyazawa Takeshi, Nakamura Takemichi, Moriya Takashi, Hirano Hiroyuki, Osada Hiroyuki, Iwabuchi Yoshiharu, Takahashi Shunji
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 18023-18023
  • DOI: 10.1038/s41598-019-54473-8
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays. (2019)
  • Author(s): Kwong MMY, Lee JW, Samian MR, Watanabe N, Osada H, Ong EBB.
  • Journal Title: J Microbiol Methods Volume: 167 Pages: 105743-105743
  • DOI: 10.1016/j.mimet.2019.105743
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] YO-001A, a new antifungal agent produced by Streptomyces sp. YO15-A001. (2019)
  • Author(s): Yamamoto K, Futamura Y, Uson-Lopez RA, Aono H, Shimizu T, Osada H.
  • Journal Title: J Antibiot Volume: 72 Issue: 12 Pages: 986-990
  • DOI: 10.1038/s41429-019-0239-z
  • Peer Reviewed
• [Journal Article] Nocardamin glucuronide, a new member of the ferrioxamine siderophores isolated from the ascamycin-producing strain Streptomyces sp. 80H647. (2019)
  • Author(s): Lopez JAV, Nogawa T, Futamura Y, Shimizu T, Osada H.
  • Journal Title: J Antibiot Volume: 72 Issue: 12 Pages: 991-995
  • DOI: 10.1038/s41429-019-0217-5
  • Peer Reviewed
• [Journal Article] Structure and biological activity of metarhizin C, a new stereoisomer of BR-050 from a fungus Tolypocladium album RK17-F0007 (2019)
  • Author(s): Nogawa T., Kawatani M., Okano A., Futamura Y., Aono H., Shimizu T., Kato N., Kikuchi H., and Osada H.
  • Journal Title: J. Antibiot. Volume: 72 Issue: 12 Pages: 996-1000
  • DOI: 10.1038/s41429-019-0229-1
  • Peer Reviewed
• [Journal Article] 2-Sulfonylpyrimidines target the kinesin HSET via cysteine alkylation. (2019)
  • Author(s): Forster T, Shang E, Shimizu K, Sanada E, Scholermann B, Huebecker B, Hahne G, Lopez-Alberca MP, Janning P, Watanabe N, Sievers S, Giordanetto F, Shimizu T, Ziegler S, Osada H, Waldmann H.
  • Journal Title: Eur J Org Chem Volume: 2019 Issue: 31-32 Pages: 5486-5496
  • DOI: 10.1002/ejoc.201900586
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stereocomplementary chemoenzymatic Pictet-Spengler reactions for formation of rare azepino-indole frameworks: Discovery of antimalarial compounds. (2019)
  • Author(s): Cai Y, Shao N, Xie H, Futamura Y, Panjikar S, Liu H, Zhu H, Osada H, Zou H.
  • Journal Title: ACS Catal Volume: 9 Issue: 8 Pages: 7443-7448
  • DOI: 10.1021/acscatal.9b01628
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Abscisic Acid Derivatives with Different Alkyl Chain Lengths Activate Distinct Abscisic Acid Receptor Subfamilies (2019)
  • Author(s): Yoshida Kazuko、Kondoh Yasumitsu、Iwahashi Fukumatsu、Nakano Takeshi、Honda Kaori、Nagano Eiki、Osada Hiroyuki
  • Journal Title: ACS Chemical Biology Volume: 14 Issue: 9 Pages: 1964-1971
  • DOI: 10.1021/acschembio.9b00453
  • Peer Reviewed
• [Journal Article] Mechanism of Action of Prethioviridamide, an Anticancer Ribosomally Synthesized and Post-Translationally Modified Peptide with a Polythioamide Structure (2019)
  • Author(s): Takase Shohei、Kurokawa Rumi、Kondoh Yasumitsu、Honda Kaori、Suzuki Takehiro、Kawahara Teppei、Ikeda Haruo、Dohmae Naoshi、Osada Hiroyuki、Shin-ya Kazuo、Kushiro Tetsuo、Yoshida Minoru、Matsumoto Ken
  • Journal Title: ACS Chemical Biology Volume: 14 Issue: 8 Pages: 1819-1828
  • DOI: 10.1021/acschembio.9b00410
  • Peer Reviewed / Open Access
• [Journal Article] Cucurbitacin B induces neurogenesis in PC12 cells and protects memory in APP/PS1 mice. (2019)
  • Author(s): Li J, Sun K, Muroi M, Gao L, Chang YT, Osada H, Xiang L, Qi J.
  • Journal Title: J Cell Mol Med Volume: 23 Issue: 9 Pages: 6283-6294
  • DOI: 10.1111/jcmm.14514
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a chemical modulator of EZH2-mediated silencing by cell-based high-throughput screening assay (2019)
  • Author(s): Murashima Akihiro、Shinjo Keiko、Katsushima Keisuke、Onuki Tetsuo、Kondoh Yasumitsu、Osada Hiroyuki、Kagaya Noritaka、Shin-ya Kazuo、Kimura Hiroshi、Yoshida Minoru、Murakami Shingo、Kondo Yutaka.
  • Journal Title: The Journal of Biochemistry Volume: - Issue: 1 Pages: 1-10
  • DOI: 10.1093/jb/mvz007
  • NAID: 40021952184
  • Peer Reviewed / Open Access
• [Journal Article] Structure characterization and action mechanism of an antiaging new compound from Gastrodia elata Blume. (2019)
  • Author(s): Farooq U, Pan Y, Lin Y, Wang Y, Osada H, Xiang L, Qi J.
  • Journal Title: Oxid Med Cell Longev Volume: 2019 Pages: 5459862-5459862
  • DOI: 10.1155/2019/5459862
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] β-carboline biomediators induce reveromycin production in Streptomyces sp. SN-593. (2019)
  • Author(s): Panthee S., Takahashi S.*, Hayashi T., Shimizu T, and Osada H.
  • Journal Title: Sci. Rep. Volume: 9 Issue: 1 Pages: 5802-5802
  • DOI: 10.1038/s41598-019-42268-w
  • Peer Reviewed / Open Access
• [Journal Article] Discovery and applications of nucleoside antibiotics beyond polyoxin. (2019)
  • Author(s): Osada H.
  • Journal Title: J Antibiot Volume: 72 Issue: 12 Pages: 853-854
  • DOI: 10.1038/s41429-019-0237-1
• [Journal Article] A novel inhibitor of tumorspheres reveals the activation of the serine biosynthetic pathway upon mitochondrial inhibition. (2019)
  • Author(s): Subedi A, Muroi M, Futamura Y, Kawamura T, Aono H, Nishi M, Ryo A, Watanabe N, Osada H.
  • Journal Title: FEBS Lett. Volume: 593 Issue: 8 Pages: 763-776
  • DOI: 10.1002/1873-3468.13361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 3β,23,28-Trihydroxy-12-oleanene 3β-caffeate from Desmodium sambuense-induced neurogenesis in PC12 cells mediated by ER stress and BDNF-TrkB signaling pathways. (2019)
  • Author(s): Cheng L, Muroi M, Cao S, Bian L, Osada H, Xiang L, Qi J
  • Journal Title: Mol Pharm Volume: 16 Issue: 4 Pages: 1423-1432
  • DOI: 10.1021/acs.molpharmaceut.8b00939
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A small-molecule ligand of valosin-containing protein/p97 inhibits cancer cell-accelerated fibroblast migration. (2019)
  • Author(s): Suvarna K, Honda K, Muroi M, Kondoh Y, Osada H, Watanabe N
  • Journal Title: J Biol Chem Volume: 294 Issue: 9 Pages: 2988-2996
  • DOI: 10.1074/jbc.ra118.004741
  • Peer Reviewed / Open Access
• [Journal Article] Using BEAN-counter to quantify genetic interactions from multiplexed barcode sequencing experiments (2019)
  • Author(s): Simpkins SW, Deshpande R, Nelson J, Li SC, Piotrowski JS, Ward HN, Yashiroda Y, Osada H, Yoshida M, Boone C, Myers CL
  • Journal Title: Nature Protocols Volume: 14 Issue: 2 Pages: 415-440
  • DOI: 10.1038/s41596-018-0099-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Induction of Nectriapyrone Biosynthesis in the Rice Blast Fungus Pyricularia oryzae by Disturbance of the Two-Component Signal Transduction System (2019)
  • Author(s): Motoyama Takayuki、Nogawa Toshihiko、Hayashi Toshiaki、Hirota Hiroshi、Osada Hiroyuki
  • Journal Title: ChemBioChem Volume: 20 Issue: 5 Pages: 693-700
  • DOI: 10.1002/cbic.201800620
  • Peer Reviewed
• [Journal Article] Construction of a potato fraction library for the investigation of functional secondary metabolites (2019)
  • Author(s): Nogawa T, Futamura Y, Okano A, Suto M, Nakamura J, Ishihara K, Osada H.
  • Journal Title: Biosci Biotechnol Biochem Volume: 83 Issue: 1 Pages: 65-75
  • DOI: 10.1080/09168451.2018.1525273
  • Peer Reviewed
• [Journal Article] Bioenergetic and proteomic profiling to screen small molecule inhibitors that target cancer metabolisms. (2019)
  • Author(s): Futamura Y, Muroi M, Aono H, Kawatani M, Hayashida M, Sekine T, Nogawa T, Osada H.
  • Journal Title: Biochim Biophys Acta Proteins Proteom. Volume: 1867 Issue: 1 Pages: 28-37
  • DOI: 10.1016/j.bbapap.2018.06.001
  • Peer Reviewed
• [Journal Article] Catenulisporolides, glycosylated triene macrolides from the chemically underexploited actinomycete Catenulispora species. (2018)
  • Author(s): Son S., Hong Y.S., Futamura Y., Jang M., Lee J.K., Heo K.T., Ko S.K., Lee J.S., Takahashi S., Osada H., Jang J.H., Ahn J.S.
  • Journal Title: Org. Lett. Volume: 20 Issue: 22 Pages: 7234-7238
  • DOI: 10.1021/acs.orglett.8b03160
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactions (2018)
  • Author(s): Simpkins SW, Nelson J, Deshpande R, Li SC, Piotrowski JS, Wilson EH, Gebre AA, Safizadeh H, Okamoto R, Yoshimura M, Costanzo M, Yashiroda Y, Ohya Y, Osada H, Yoshida M, Boone C, Myers CL
  • Journal Title: PLoS Computational Biology Volume: 14 Issue: 10 Pages: e1006532-e1006532
  • DOI: 10.1371/journal.pcbi.1006532
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structures and synthesis of hitoyopodins: bioactive aromatic sesquiterpenoids produced by the mushroom Coprinopsis cinerea. (2018)
  • Author(s): Otaka J, Shimizu T, Futamura Y, Hashizume D, Osada H.
  • Journal Title: Org Lett Volume: 20 Issue: 19 Pages: 6294-6297
  • DOI: 10.1021/acs.orglett.8b02788
  • Peer Reviewed
• [Journal Article] Catalytic enantioselective synthesis of a pyrrolizidine-alkaloid-inspired compound collection with antiplasmodial activity. (2018)
  • Author(s): Jia ZJ, Takayama H, Futamura Y, Aono H, Bauer JO, Strohmann C, Antonchick AP, Osada H, Waldmann H.
  • Journal Title: J Org Chem Volume: 83 Issue: 13 Pages: 7033-7041
  • DOI: 10.1021/acs.joc.7b03202
  • Peer Reviewed
• [Journal Article] Anti-angiogenesis effects induced by octaminomycins A and B against HUVECs. (2018)
  • Author(s): Jang JP, Han JM, Jung HJ, Osada H, Jang JH, Ahn JS.
  • Journal Title: J Microbiol Biotechnol Volume: 28 Pages: 1332-1338
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of an Asexual Reproduction Inducer of Phytopathogenic and Toxigenic Fusarium. (2018)
  • Author(s): Jianhua Qi, Lihong Cheng, Yujuan Sun, Yushi Hirata, Naoki Ushida, Zhonghua Ma, Hiroyuki Osada, Toshio Nishikawa, Lan Xiang.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 57 Issue: 27 Pages: 8100-8104
  • DOI: 10.1002/anie.201803329
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of cytotoxic, glutathione-reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion (2018)
  • Author(s): Wilke J, Kawamura T, Watanabe N, Osada H, Ziegler S, Waldmann H.
  • Journal Title: Bioorg Med Chem Volume: 26 Issue: 8 Pages: 1453-1461
  • DOI: 10.1016/j.bmc.2017.11.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pentaminomycins A and B, hydroxyarginine-containing cyclic pentapeptides from Streptomyces sp. RK88-1441. (2018)
  • Author(s): Jang J.-P., Hwang G.J., Kwon M.C., Ryoo I.-J., Jang M., Takahashi S., Ko S.K., Osada H., Jang J.H., and Ahn J.S.
  • Journal Title: J. Nat. Prod. Volume: 81 Issue: 4 Pages: 806-810
  • DOI: 10.1021/acs.jnatprod.7b00882
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of a terpenoid-production platform in Streptomyces reveromyceticus SN-593 (2017)
  • Author(s): Khalid A、Takagi H、Panthee S、Muroi M、Chappell J、Osada H、Takahashi S
  • Journal Title: ACS Synthetic Biology Volume: 6 Issue: 12 Pages: 2339-2349
  • DOI: 10.1021/acssynbio.7b00249
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phenotypic identification of a novel autophagy inhibitor chemotype targeting lipid kinase VPS34 (2017)
  • Author(s): Robke L, Laraia L, Carnero Corrales MA, Konstantinidis G, Muroi M, Richters A, Winzker M, Engbring T, Tomassi S, Watanabe N, Osada H, Rauh D, Waldmann H, Wu YW, Engel J
  • Journal Title: Angew Chem Int Ed Engl Volume: 56 Issue: 28 Pages: 8153-8157
  • DOI: 10.1002/anie.201703738
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Allantopyrone A activates the Keap1-Nrf2 pathway and protects from oxidative stress-induced cell death in PC12 cells (2017)
  • Author(s): Shota Uesugi, Makoto Muroi, Yasumitsu Kondoh, Yoshihito Shiono, Hiroyuki Osada, Ken-ichi Kimura
  • Journal Title: The Journal of Antibiotics Volume: 70 Issue: 4 Pages: 429-434
  • DOI: 10.1038/ja.2016.99
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure-activity relationships of terpendole E and its natural derivatives. (2017)
  • Author(s): Nagumo Y, Hayashi T, Hirota H, Aono H, Kawatani M, Osada H, Usui T
  • Journal Title: ChemistrySelect Volume: 2 Issue: 4 Pages: 1533-1536
  • DOI: 10.1002/slct.201602015
  • Peer Reviewed
• [Journal Article] Functional annotation of chemical libraries across diverse biological processes. (2017)
  • Author(s): Piotrowski JS, Li SC, Deshpande R.......Shirahige K,......Boone C.
  • Journal Title: Nat Chem Biol. Volume: 13 Issue: 9 Pages: 982-993
  • DOI: 10.1038/nchembio.2436
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification and structure-activity relationship of purine derivatives as novel MTH1 inhibitors. (2016)
  • Author(s): Kumar A, Kawamura T, Kawatani M, Osada H, Zhang KY
  • Journal Title: Chem Biol Drug Des Volume: - Issue: 6 Pages: 862-869
  • DOI: 10.1111/cbdd.12909
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 微生物に新規化合物を求めて (2021)
  • Author(s): 長田裕之
  • Organizer: 日本生物工学会
  • Invited
• [Presentation] 天然物ケミカルバイオロジー： 微生物はミクロの錬金術師である (2021)
  • Author(s): 長田裕之
  • Organizer: 10th INA Chemical Biology Seminar
  • Invited
• [Presentation] 抗がん剤開発を目指したケミカルバイオロジー研究 (2021)
  • Author(s): 長田裕之
  • Organizer: 新規素材探索研究会
  • Invited
• [Presentation] 天然物創薬に適した表現型スクリーニング (2021)
  • Author(s): 長田裕之
  • Organizer: 日本農芸化学会東北支部 第155回大会
  • Invited
• [Presentation] Induction of biosynthesis of secondary metabolites in the rice blast fungus Pyricularia oryzae. (2021)
  • Author(s): Hiroyuki Osada
  • Organizer: PACIFICHEM2021
  • Int'l Joint Research / Invited
• [Presentation] NPD10084, a small molecule inhibitor of PKM2, identified by the newly developed 2DE-CETSA. (2021)
  • Author(s): Hiroyuki Osada
  • Organizer: PACIFICHEM2021
  • Int'l Joint Research / Invited
• [Presentation] 天然物創薬に適した表現系スクリーニング (2021)
  • Author(s): 長田 裕之
  • Organizer: 日本農芸化学会東北支部 第155回大会
  • Invited
• [Presentation] Target identification of NPD10084, a novel inhibitor of cancer metabolism. (2020)
  • Author(s): Osada, H.
  • Organizer: Symposium on Convergent technologies for biomedical impact.
  • Int'l Joint Research / Invited
• [Presentation] Screening of new antifungal antibiotics using artificial intelligence. (2019)
  • Author(s): Osada, H.
  • Organizer: 日中分野別ハイレベル研究者交流会2019：医薬・健康領域
  • Int'l Joint Research / Invited
• [Presentation] Target identification of small molecule compounds. (2019)
  • Author(s): Osada, H
  • Organizer: 浙江大学薬学院明徳弘薬セミナー
  • Int'l Joint Research / Invited
• [Presentation] Morphology-based screening of antitumor and antifungal compounds. (2019)
  • Author(s): Osada, H.
  • Organizer: 8th Annual Conference ICBS-2019
  • Int'l Joint Research / Invited
• [Presentation] Biosynthesis of tenuazonic acid in Pyricularia oryzae. (2019)
  • Author(s): Osada, H
  • Organizer: The Cold Spring Harbor Asia conference on Chemical Biology and Drug Discovery.
  • Int'l Joint Research / Invited
• [Presentation] Screening of new antifungal compounds based on morphological change of fungi. (2019)
  • Author(s): Osada, H
  • Organizer: The 2nd International Symposium on Natural Resources-based Drug Development.
  • Int'l Joint Research / Invited
• [Presentation] アカデミア創薬の難しさ (2019)
  • Author(s): 長田裕之
  • Organizer: 第14回 日本がん分子標的治療学会 TRワークショップ
  • Invited
• [Presentation] Ring formation in biosynthesis of specialized microbial metabolites (2019)
  • Author(s): Hiroyuki Osada
  • Organizer: 2nd China-Japan Joint Symposium on Natural Product Biosynthesis
  • Int'l Joint Research / Invited
• [Presentation] ケミカルバイオロジー基盤を活用した創薬シードの探索 (2018)
  • Author(s): 長田裕之
  • Organizer: 講演会「化学が先導するライフ・イノベーション ～医療・創薬研究の最前線～」
  • Invited
• [Presentation] マルチオミックス基盤を活用したがん代謝阻害剤の探索 (2018)
  • Author(s): 長田裕之
  • Organizer: 化学コミュニケーションのフロンティア 第3回公開シンポジウム
  • Invited
• [Presentation] がん代謝阻害剤の探索 (2018)
  • Author(s): 長田裕之
  • Organizer: 愛媛大学大学医学部付属病院先端医療創生センターTRCシリーズセミナー
  • Invited
• [Presentation] Timing of the mycotoxin, tenuazonic acid, produciton at the infection of plant pathogenic fungi (2018)
  • Author(s): Hiroyuki Osada
  • Organizer: The Japanese-German Symposium
  • Int'l Joint Research / Invited
• [Presentation] Target identification of compounds isolated from a fraction library of fungal broths (2018)
  • Author(s): Hiroyuki Osada, Toshihiko Nogawa, Yushi Futamura, Makoto Muroi, Naoki Kato
  • Organizer: 3rd European Conference on Natural Products 2018
  • Int'l Joint Research / Invited
• [Presentation] 破骨細胞を標的とするリベロマイシンA：生物活性研究から生合成研究への展開 (2018)
  • Author(s): 長田 裕之
  • Organizer: 日本薬学会第138回年会
  • Invited
• [Presentation] 抗生物質学からケミカルバイオロジーへの展開（微生物創薬研究の温故知新） (2018)
  • Author(s): 長田 裕之
  • Organizer: 日本技術士会化学部会 2月度 講演会
  • Invited
• [Presentation] Systematic isolation of microbial metabolites and the target identification (2018)
  • Author(s): Hiroyuki Osada
  • Organizer: International Symposium on Himalayan Biodiversity Bioresources
  • Int'l Joint Research / Invited
• [Presentation] Anti-osteoclastic agent, reveromycin A (biological activity and biosynthesis) (2018)
  • Author(s): Hiroyuki Osada
  • Organizer: Department of Chemistry Bicoastal Lecture Series, The Scripps Research Institute
  • Int'l Joint Research / Invited
• [Presentation] Chemical synthesis or biosynthesis:It is the question for reveromycin production (2018)
  • Author(s): Hiroyuki Osada
  • Organizer: The Mona Symposium 2018
  • Int'l Joint Research / Invited
• [Presentation] がん代謝を標的とする小分子阻害剤の探索 (2017)
  • Author(s): 長田 裕之、二村 友史、室井 誠
  • Organizer: 第76回日本癌学会学術総会
  • Invited
• [Presentation] 破骨細胞を標的とするreveromycin Aの研究 (2017)
  • Author(s): 長田 裕之
  • Organizer: 筑波大学WPIセミナー「有機化学・創薬化学セミナー」
  • Invited
• [Presentation] 抗生物質学からケミカルバイオロジーへの展開（微生物創薬研究の温故知新） (2017)
  • Author(s): 長田 裕之
  • Organizer: ライフサイエンス技術部会・反応分科会 講演会
  • Invited
• [Presentation] 的を射る難しさ (2017)
  • Author(s): 長田 裕之
  • Organizer: 第52回 天然物化学談話会
  • Invited
• [Presentation] ケミカルバイオロジーの威力 (2017)
  • Author(s): 長田 裕之
  • Organizer: 関西学院大学化学セミナー
  • Invited
• [Presentation] Chemical array screening, a powerful approach for exploring small molecule inhibitors against proteins (2017)
  • Author(s): Hiroyuki Osada
  • Organizer: International Symposium “Systems Glycobiology and Beyond”
  • Int'l Joint Research / Invited
• [Presentation] Screening and target identification of small molecules affecting cancer metabolism (2017)
  • Author(s): Hiroyuki Osada
  • Organizer: ICBS 2017
  • Int'l Joint Research / Invited
• [Presentation] Activation of the sleeping gene cluster of microbial secondary metabolites (2017)
  • Author(s): Hiroyuki Osada
  • Organizer: 9th US-Japan Seminar on the Biosynthesis of Natural Products
  • Int'l Joint Research / Invited
• [Presentation] Screening of inhibitors against cancer stem cells from natural products depository (NPDepo) (2017)
  • Author(s): Hiroyuki Osada
  • Organizer: 18th International Symposium on the Biology of Actinomycetes (ISBA18)
  • Int'l Joint Research / Invited
• [Presentation] Screening of small molecule inhibitors of human pirin (2017)
  • Author(s): Hiroyuki Osada
  • Organizer: 253rd American Chemical Society National Meeting & Exposition
  • Int'l Joint Research / Invited
• [Book] 膨れた細胞に微笑んだ幸運の女神, マウス・ラット モデル動物作製・解析プロフェッショナル (2021)
  • Author(s): 長田裕之
  • Total Pages: 1
  • Publisher: 羊土社
• [Book] オミックス解析：表現型・薬物活性とリンクする分子経路の探索, マウス・ラット モデル動物作製・解析プロフェッショナル (2021)
  • Author(s): 室井 誠, 馬島 哲夫, 牛嶋 大, 松浦正明
  • Total Pages: 11
  • Publisher: 羊土社
• [Book] マウス・ラットモデル作製・解析プロフェッショナル (2021)
  • Author(s): 先端モデル動物支援プラットフォーム（AdAMS）
  • Total Pages: 320
  • Publisher: 羊土社
  • ISBN: 9784758121125
• [Book] Protein-protein Interaction regulators. Drug discovery series No. 78 (2021)
  • Author(s): Roy S. and Fu H (eds.)
  • Total Pages: 380
  • Publisher: Royal society of chemistry
  • ISBN: 9781788011877
• [Book] Comprehensive natural products III: chemistry and biology. vol. 7 (2020)
  • Author(s): Liu HB and Begley TP (eds.)
  • Total Pages: 553
  • Publisher: Elsevier
  • ISBN: 9780081026915
• [Book] 医薬品開発におけるオミクス解析技術 ～ゲノム・トランスクリプトーム・プロテオーム・メタボローム～ (2020)
  • Author(s): 鎌田 真由美, 奥野 恭史, 山田 弘, 森田 英利, 田丸 浩, 伊藤 昌可, 林崎 良英, 飯塚 朋代, 小林 巧, 池尾 一穂, 中岡 慎治, 松田 史生, 室井 誠, 小川 直子, 長田 裕之, 三浦 信明, 杉本 昌弘, 中山 泰宗, 三枝 淳, 松本 光晴, 久保田 浩行, 金井 弥栄, 津田 昇, 鈴木 孝昌
  • Total Pages: 181
  • Publisher: 情報機構
  • ISBN: 9784865021844
• [Book] Systems Chemical Biology, Methods and Protocols, Methods in Molecular Biology (2019)
  • Author(s): Slava Ziegler and Herbert Waldmann
  • Total Pages: 315
  • Publisher: Springer
• [Book] ケミカルバイオロジー化合物集 -研究展開のヒント - (2018)
  • Author(s): 日本学術振興会ケミカルバイオロジー第189委員会」編 編集長：長田 裕之
  • Publisher: 株式会社 オーム社
• [Book] Bioprobes (2017)
  • Author(s): Osada, Hiroyuki (Ed.)
  • Total Pages: 384
  • Publisher: Springer Japan
  • ISBN: 9784431565277
• [Remarks] 理化学研究所 環境資源科学研究センター ケミカルバイオロジー研究グループ
  • URL: http://www.cbrg.riken.jp/csrs/ja/
• [Remarks] 理化学研究所 環境資源科学研究センター ケミカルバイオロジーグループ
  • URL: http://www.cbrg.riken.jp/csrs/ja/
• [Remarks] 理化学研究所 環境資源科学研究センター ケミカルバイオロジー グループ
  • URL: http://www.cbrg.riken.jp
• [Remarks] 理化学研究所 環境資源科学研究センター ケミカルバイオロジー グループ
  • URL: http://www.cbrg.riken.jp/csrs/ja/
• [Patent(Industrial Property Rights)] 新規フラノクマリン誘導体及びその用途 (2022)
  • Inventor(s): 川谷誠、長田裕之、青野晴美、平沼佐代子、清水猛
  • Industrial Property Rights Holder: 川谷誠、長田裕之、青野晴美、平沼佐代子、清水猛
  • Industrial Property Rights Type: 特許
  • Filing Date: 2022
• [Patent(Industrial Property Rights)] イネいもち病防除薬剤 (2019)
  • Inventor(s): 長田裕之, 本山高幸, 清水猛, 近藤恭光
  • Industrial Property Rights Holder: 理研
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Funded Workshop] 新学術領域研究「化学コミュニケーションのフロンティア」第1回国際シンポジウム The 1st International Symposium on Chemical Communication (ISCC2019) (2019)