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Sexual spectrum of fish brain

Planned Research

Project AreaSpectrum of the Sex: a continuity of phenotypes between female and male
Project/Area Number 17H06429
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

Okubo Kataaki  東京大学, 大学院農学生命科学研究科(農学部), 教授 (10370131)

Co-Investigator(Kenkyū-buntansha) 中城 光琴  大阪医科薬科大学, 医学部, 助教 (60824795)
Project Period (FY) 2017-06-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥124,930,000 (Direct Cost: ¥96,100,000、Indirect Cost: ¥28,830,000)
Fiscal Year 2021: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2020: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2019: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2018: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2017: ¥37,050,000 (Direct Cost: ¥28,500,000、Indirect Cost: ¥8,550,000)
Keywords性スペクトラム / 魚類 / 脳 / 行動 / 脳・神経 / 生理学 / 細胞・組織 / 神経科学
Outline of Final Research Achievements

Teleost fish are unique among vertebrates in that their sex-specific phenotypes, including behaviors, are quite labile and can even be reversed as adults. This indicates that the fish brain can transition from one end of the sexual spectrum to the other throughout their lifetime. In medaka (Oryzias latipes), we recently identified a group of neurons in the brain region implicated in mating behavior that appear and disappear in a reversible manner in response to changes in the adult sex steroid milieu. The results of this study strongly suggest that these neurons underpin the mechanisms that allow the fish brain (mating behavior pattern) to transition on the sexual spectrum and also revealed part of the molecular and cellular basis of these mechanisms.

Academic Significance and Societal Importance of the Research Achievements

本研究は、体内のホルモン環境に応じて性スペクトラム上の位置が変わる魚類の脳に焦点を当て、魚類の脳(性行動パターン)が性スペクトラム上を大きく移動できるメカニズムの一端を明らかにした。研究をさらに進めることで、脳の性の確かさや変わりやすさの分子基盤がみえてくると期待される。魚類は生涯にわたって性転換する能力を有していることが知られており、性転換に伴って、脳の性も雌雄逆転する。本研究の成果は、未だ謎に包まれている魚類の性転換メカニズムを明らかにする糸口としても注目に値する。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (25 results)

All 2021 2020 2019 2018 2017

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (17 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Journal Article] Estrogen mediates sex differences in preoptic neuropeptide and pituitary hormone production in medaka2021

    • Author(s)
      Yamashita J, Nishiike Y, Fleming T, Kayo D, Okubo K
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 948-948

    • DOI

      10.1038/s42003-021-02476-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estrogen receptor 2b is the major determinant of sex-typical mating behavior and sexual preference in medaka2021

    • Author(s)
      Nishiike Yuji、Miyazoe Daichi、Togawa Rie、Yokoyama Keiko、Nakasone Kiyoshi、Miyata Masayoshi、Kikuchi Yukiko、Kamei Yasuhiro、Todo Takeshi、Ishikawa-Fujiwara Tomoko、Ohno Kaoru、Usami Takeshi、Nagahama Yoshitaka、Okubo Kataaki
    • Journal Title

      Current Biology

      Volume: 31 Issue: 8 Pages: 1-12

    • DOI

      10.1016/j.cub.2021.01.089

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Male-predominant galanin mediates androgen-dependent aggressive chases in medaka2020

    • Author(s)
      Yamashita Junpei、Takeuchi Akio、Hosono Kohei、Fleming Thomas、Nagahama Yoshitaka、Okubo Kataaki
    • Journal Title

      eLife

      Volume: 9

    • DOI

      10.7554/elife.59470

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Androgen-dependent sexual dimorphism in pituitary tryptophan hydroxylase expression: relevance to sex differences in pituitary hormones2020

    • Author(s)
      Kawabata-Sakata Yukika、Nishiike Yuji、Fleming Thomas、Kikuchi Yukiko、Okubo Kataaki
    • Journal Title

      Proceedings of the Royal Society B: Biological Sciences

      Volume: 287 Issue: 1928 Pages: 20200713-20200713

    • DOI

      10.1098/rspb.2020.0713

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuropeptide B mediates female sexual receptivity in medaka fish, acting in a female-specific but reversible manner2019

    • Author(s)
      Hiraki-Kajiyama T, Yamashita J, Yokoyama K, Kikuchi Y, Nakajo M, Miyazoe D, Nishiike Y, Ishikawa K, Hosono K, Kawabata-Sakata Y, Ansai S, Kinoshita M, Nagahama Y, Okubo K
    • Journal Title

      eLife

      Volume: 8

    • DOI

      10.7554/elife.39495

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A conceptual framework for understanding sexual differentiation of the teleost brain2019

    • Author(s)
      Okubo K, Miyazoe D, Nishiike Y
    • Journal Title

      General and Comparative Endocrinology

      Volume: in press Pages: 113129-113129

    • DOI

      10.1016/j.ygcen.2019.02.020

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sexually Dimorphic Neuropeptide B Neurons in Medaka Exhibit Activated Cellular Phenotypes Dependent on Estrogen2019

    • Author(s)
      Yukiko Kikuchi, Towako Hiraki-Kajiyama, Mikoto Nakajo, Chie Umatani, Shinji Kanda, Yoshitaka Oka, Keisuke Matsumoto, Hitoshi Ozawa, Kataaki Okubo
    • Journal Title

      Endocrinology

      Volume: 160 Issue: 4 Pages: 827-839

    • DOI

      10.1210/en.2019-00030

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of isotocin is male-specifically upregulated by gonadal androgen in the medaka brain2017

    • Author(s)
      Yamashita J, Kawabata Y, Okubo K
    • Journal Title

      J Neuroendocrinol

      Volume: 29 Issue: 12

    • DOI

      10.1111/jne.12545

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] How do evolutionarily conserved neural substrates give rise to variation in social behaviors across species?2021

    • Author(s)
      Okubo K
    • Organizer
      Japan-Norway Symposium on Fish Endocrinology and Genomics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Estrogen receptor 2b plays an essential role in establishing female mating behavior in medaka2021

    • Author(s)
      Nishiike Y, Okubo K
    • Organizer
      Japan-Norway Symposium on Fish Endocrinology and Genomics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prostaglandin receptor 4b (ptger4b) regulates female receptivity in medaka2021

    • Author(s)
      Fleming T, Okubo K
    • Organizer
      Japan-Norway Symposium on Fish Endocrinology and Genomics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 性ステロイド環境に応じてメダカの性行動パターンをメス型化する脳内メカニズム2021

    • Author(s)
      西池雄志、大久保範聡
    • Organizer
      第45回日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 魚類の性行動パターンの性差形成と性的可逆性2019

    • Author(s)
      大久保範聡
    • Organizer
      日本内分泌学会 第37回内分泌代謝学サマーセミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] メス特異的なNpb発現を示すペプチドニューロンの性行動制御における役割2019

    • Author(s)
      中城 光琴、平木(梶山)十和子、大久保 範聡
    • Organizer
      日本動物学会第90回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Sexually dimorphic neuropeptide B neurons in the ventral telencephalon, a leading candidate for the regulator of female reproductive behavior, show estrogen dependence and diurnal changes in their neuronal activities2019

    • Author(s)
      Nakajo M, Hiraki-Kajiyama T, Okubo K
    • Organizer
      Neuroscience 2019 (Society for Neuroscience)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 終脳腹側野においてメス特異的なNpb発現を示すペプチドニューロンの神経生理学的解析2018

    • Author(s)
      中城光琴、平木(梶山)十和子、大久保範聡
    • Organizer
      第43回日本比較内分泌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカのメス特異的Npbニューロンの遺伝子発現プロファイルと機能の解析2018

    • Author(s)
      菊池結貴子、大久保範聡
    • Organizer
      第43回日本比較内分泌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカの脳と行動の性分化における性ステロイドの役割2018

    • Author(s)
      宮副大地、西池雄志、大久保範聡
    • Organizer
      第43回日本比較内分泌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカにおける攻撃行動に寄与する神経核の探索2018

    • Author(s)
      西池雄志、宮副大地、大久保範聡
    • Organizer
      第43回日本比較内分泌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカの視索前野に存在するメス特異的な性ステロイド応答性ニューロンの活性化に関わる遺伝子の探索2018

    • Author(s)
      立澤雅也、大久保範聡
    • Organizer
      第43回日本比較内分泌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 魚類の性の不思議な世界2018

    • Author(s)
      大久保範聡
    • Organizer
      第11回日本性差医学・医療学会学術集会(公開シンポジウム「性差生物学の最近の進歩:進化の過程での性差」)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] エストロゲン受容体Esr2bノックアウトメダカの性行動と生殖腺における表現型解析2018

    • Author(s)
      宮副大地・大久保範聡
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 真骨魚類の脳の性分化・性的可逆性に関する研究2017

    • Author(s)
      大久保範聡
    • Organizer
      第42回日本比較内分泌学会(日本比較内分泌学会奨励賞受賞者講演)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] メダカにおけるメス特異的なペプチド産生ニューロンの性ステロイド応答性と性的可逆性2017

    • Author(s)
      菊池結貴子・大久保範聡
    • Organizer
      第42回日本比較内分泌学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] エストロゲン受容体ノックアウトメダカを用いた魚類の行動の性差形成メカニズムの解析2017

    • Author(s)
      宮副大地・大久保範聡
    • Organizer
      第42回日本比較内分泌学会
    • Related Report
      2017 Annual Research Report

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Published: 2017-07-04   Modified: 2023-01-30  

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