| Budget Amount *help |
¥110,500,000 (Direct Cost: ¥85,000,000、Indirect Cost: ¥25,500,000)
Fiscal Year 2021: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2020: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2019: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2018: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2017: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Outline of Final Research Achievements |
Through this funding, we unraveled the fundamental mechanism and regulatory circuitry specifying both transient and perpetual stem cells (stomatal meristemoids and vascular stem cells, respectively). We discovered that MUTE, a master regulator of stomatal fate commitment, drives the transition from stem-cell to differentiation through direct regulation of cell cycle gene expression. MUTE enables the deceleration of rapidly dividing stomatal precursors to execute slow, precise terminal symmetric division, which is critical for proper guard cell identity. The MUTE-orchestrated feed-forward loop enables the robust execution of the single symmetric division to generate a functional stoma with paired guard cells. We developed the VISUAL system to visualize dynamic cell-fate acquisition of vascular stem cells. Leveraged by this system, we discovered that a competition among BES/BZR-family of transcription factor stabilize the stemness of vascular cambiums.
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