Mechanism of growth and virulence expression in intracellular parasitic bacteria and mycobacteria

• Project Area: Matrix of Infection Phenomena
• Project/Area Number: 18073009
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: MITSUYAMA Masao Kyoto University, 医学研究科, 教授 (10117260)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥95,000,000 (Direct Cost: ¥95,000,000); Fiscal Year 2010: ¥19,000,000 (Direct Cost: ¥19,000,000); Fiscal Year 2009: ¥19,000,000 (Direct Cost: ¥19,000,000); Fiscal Year 2008: ¥19,000,000 (Direct Cost: ¥19,000,000); Fiscal Year 2007: ¥19,000,000 (Direct Cost: ¥19,000,000); Fiscal Year 2006: ¥19,000,000 (Direct Cost: ¥19,000,000)
• Keywords: 細胞内寄生菌 / リステリア / 結核菌病原因子 / マクロファージ / サイトカイン / 抗酸菌 / 病原性 / 結核菌 / リステリア菌 / エスケープ機構 / 細胞性免疫

Research Abstract

This study was carried out to reveal how the virulence factors of Listeria and acid-fast bacteria, two representative intracellular parasitic bacteria, contribute to the host cytokine response. It was clearly shown that the activation of both calpain and caspase-1 in host macrophages is highly dependent on the major virulence factor, cytolysin and RD1 locus product of Listeria and Mycobacteria, respectively, resulting in the processing and secretion of IL-1α, IL-1β, and IL-18. The findings obtained in this study established the pivotal role of bacterial virulence factors for the induction of host cytokine response.

Research Products

• [Journal Article] Expressionionof the Mycobacterium tuberculosis PPE37protein in Mycobacterium smegmatis induces low TNF-a and IL-6 production in murine macrophages. (2011)
  • Author(s): Daim, S., Kawamura, I., Tsuchiya, K., Hara, H., Kurenuma, T., Shen, Y., Dewamitta, S.R., Sakai, S., Nomura, T., Qu, H., Mitsuyama, M.
  • Journal Title: J.Med.Microbiol. 60 Pages: 582-591
  • Peer Reviewed
• [Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumor necrosis factor alpha and interleukin 6 production in murine macrophages. (2011)
  • Author(s): Daim S, et al.
  • Journal Title: Journal of Medical Microbiology Volume: 60 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages. (2010)
  • Author(s): Shen, Y., Kawamura, I., Nomura, T., Tsuchiya, K., Hara, H., Dewamitta, S.R., Sakai, S., Qu, H., Daim, S., Yamamoto, T., Mitsuyama, M.
  • Journal Title: Infect.Immun. 78 Pages: 2857-2867
  • NAID: 120002381103
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Tsuchiya, K., Hara, H., Kawamura, I., Nomura, T., Yamomoto, T., Daim, S., Dewamitta, S.R., Shen, Y., Fang, R., Mitsuyama, M.
  • Journal Title: 85 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] PD-1-PD-L1pathway impairs Th1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guerin. (2010)
  • Author(s): Sakai, S., Kawamura, I., Okazaki, T., Tsuchiya, K., Uchiyama, R., Mitsuyama, M.
  • Journal Title: Int.Immunol. 22 Pages: 915-925
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for caplain activation and interleukin-1a secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Dewamitta, S.R., Nomura, T., Kawamura, I., Hara, H., Tsuchiya, K., Kurenuma, T., Shen, Y., Daim, S., Yamamoto, T., Qu, H., Sakai, S., Xu, Y., Mitsuyama, M.
  • Journal Title: Infect.Immun. 78 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] PD-1-PD-L1 pathway impairs Th1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guerin. (2010)
  • Author(s): Sakai S, et al.
  • Journal Title: International Immunology Volume: 22 Pages: 915-925
  • Peer Reviewed
• [Journal Article] Alpha galactosylceramide promotes killing of Listeria monocytogenes within the macrophage phagosome through invariant NKT-cell activation. (2010)
  • Author(s): Emoto M, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2667-2676
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytognes (2010)
  • Author(s): Tsuchiya K, et al.
  • Journal Title: Journal of Immunology Volume: 185 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2-and Myd88-dependent phosphatidylinositol 3 -kanase and Rac1 activation facilitates the phagocytosis of Listeiria monocyto-genes by murine macrophages (2010)
  • Author(s): Yanna S, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2857-2867
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Dewamitta SR, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into cytoplasm initiates calpain activation and IL-1α secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Sita R.Dewamitta, Takamasa Nomura, Ikuo Kawamura, Hideki Hara, Kohsuke Tsuchiya, Takeshi Kurenuma, Shen Yanna, Sylvia Daim, Takeshi Yamamoto, Huixin Qu, Shunsuke Sakai, Yanting Xu, Masao Mitsuvama.
  • Journal Title: Infection and Immunity 78(5)(in press)
  • Peer Reviewed
• [Journal Article] Alfa-galactosylceramide promotes killing of Listeria monocytogenes within the macrophage phagosome through invariant NKT cell activation. (2010)
  • Author(s): Masashi Emoto, Tomomi Yoshida, Toshio Fukuda, Ikuo Kawamura, Masao Mitsuyama, Eiji Kita, Robert Hurwitz, Stefan H.E.Kaufmann, Yoshiko Emoto.
  • Journal Title: Infection and Immunity (accepted)
  • Peer Reviewed
• [Journal Article] TLR2-MyD88-dependent P13K and Racl activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages. (2010)
  • Author(s): Yanna Shen, Ikuo Kawamura^*, Takamasa Nomura, Kohsuke Tsuchiya, Hideki Hara, Huixin Qu, Sita R.Dewamitta, Takeshi Yamamoto, Sylvia Daim, Shunsuke Sakai, Takeshi Kurenuma, Masao Mitsuyama
  • Journal Title: Infection and Immunity (accepted)
  • Peer Reviewed
• [Journal Article] The RD1 locus in the Mycobacterium tuberculosis genome contributes to activation of caspase-1 via induction of potassium ion efflux in infected macrophages. (2009)
  • Author(s): Kurenuma, T., Kawamura, I., Hara, H., Uchiyama, R., Daim, S., Dewamitta, S.R., Sakai, S., Tsuchiya, K., Nomura, T., Mitsuyama, M.
  • Journal Title: Infect.Immun. 77 Pages: 3992-4001
  • Peer Reviewed
• [Journal Article] The RD 1 locus in the Mycobacterium tuberculosis genome contributes to activation of caspase-1 via induction of potassiumion efflux in infected macrophages. (2009)
  • Author(s): Takeshi Kurenuma, Ikuo Kawamura, Hideki Hara, Ryosuke Uchiyama, Sylvia Daim, Sita Ramyamali Dewamitta, Shunsuke Sakai, Kohsuke Tsuchiya, Takamasa Nomura, Masao Mitsuvama.
  • Journal Title: Infection and Immunity 77 Pages: 3992-4001
  • Peer Reviewed
• [Journal Article] Kyoto University Graduate School of Medicine : Tradition and modernity harmonized. (2009)
  • Author(s): Masao Mitsuyama
  • Journal Title: Journal of Molecular Medicine 87 Pages: 1009-1013
  • Peer Reviewed
• [Journal Article] プロバイオテイクスと疾患 はじめに(編集企画) (2009)
  • Author(s): 光山正雄
  • Journal Title: 医学のあゆみ 228(3) Pages: 197-197
• [Journal Article] 細胞内寄生菌のマクロファージ内生存戦略 (2009)
  • Author(s): 土屋晃介、河村伊久雄、光山正雄
  • Journal Title: PNE蛋白質核酸酵素増刊号:感染現象(木下タロウ外編集) Pages: 996-1001
• [Journal Article] 病原因子と宿主防御機構 (2009)
  • Author(s): 光山正雄
  • Journal Title: 増刊号:感染症-ウイルス・細菌・寄生虫の感染戦略(光山、北、野本編集) 実験医学27(10) Pages: 1622-1626
  • Peer Reviewed
• [Journal Article] 細胞内寄生性リステリアの病原因子による宿主免疫応答の誘導機構-生菌免疫の作用機序へのアプローチ- (2009)
  • Author(s): 光山正雄
  • Journal Title: 日本細菌学雑誌 64(4) Pages: 365-376
  • NAID: 10027208784
  • Peer Reviewed
• [Journal Article] Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, listeriolysin O, after evasion from phagosome into the cytoplasm. (2008)
  • Author(s): Hara, H., Tsuchiya, K., Nomura, T., Kawamura, I., Shoma, S., Mitsuyama, M.
  • Journal Title: J.Immunol. 180 Pages: 7859-7868
  • Peer Reviewed
• [Journal Article] Critical involvement of pneumolysin in production of IL-1a and caspase-1-dependent cytokines in infection with Streptococcus pneumoniae in vitro: a novel function of pneumolysin in caspase-1 activation. (2008)
  • Author(s): Shoma, S., Tsuchiya, K., Kawamura, I., Nomura, T., Hara, H., Uchiyama, R., Daim, S., Mitsuyama, M.
  • Journal Title: Infect.Immun. 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, Listeriolysin O, after evasion from phagosome into the cytoplasm. (2008)
  • Author(s): Hara H, Tsuchiya K, Nomura T, Kawamura I, Shoma S & Mitsuyama M
  • Journal Title: J.Immunol. 180 Pages: 7859-7868
• [Journal Article] Critical involvement of pneumolysin in production of IL-1α and caspase-1- dependent cytokines in infection with Streptococcus pneumoniae in vitro: a novel function of pneumolysin in caspase-1 activation. (2008)
  • Author(s): Shoma S, Tsuchiya K, Kawamura I, Nomura T, Hara H, Uchiyama R, Daim S & Mitsuyama M
  • Journal Title: Infect. Immun. 76 Pages: 1547-1557
• [Journal Article] Dependency of caspase-1 activation induced inmadrophages by Listeria monocytogenes on cytolysin, Listeriolysin O, after evasion from phagosome into the cytoplasm (2008)
  • Author(s): Hara H, Tsuchiya K, Nomura T, Kawamura I, Shoma S & Mitsuyama M
  • Journal Title: J. Immunol. 180 Pages: 7859-7868
  • Peer Reviewed
• [Journal Article] Critical involvement of pneumolysin in production of IL-1α and caspase-1-dependent cytokines in infection with Streptotoccus pneumoniae in vitro : a novel function of pneumolysin in caspase-1 activation (2008)
  • Author(s): Shoma S, Tsuchiya K, Kawamura I, Nomura T, Hara H, Uchiyama R, Daim S & Mitsuvama M
  • Journal Title: Infect. Immun. 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Critical involvement of pneumolysin in production of IL-1α and caspase-1-dependent cytokines in infection with Streptococcus pneumoniae in vitro: a novel function of pneumolysin in caspase-1 activation. (2008)
  • Author(s): Shereen Shoma, et. al.
  • Journal Title: Infection and Immunity 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Dependency of Caspase-1 Activation Induced in Macrophages by Listeria monocytogenes on Cytolysin, Listeriolysin 0, after Evasion from Phagosome into the Cytoplasm. (2008)
  • Author(s): Hideki Hara, et. al.
  • Journal Title: Journla of Immunology 180 Pages: 1-11
  • Peer Reviewed
• [Journal Article] RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion. (2007)
  • Author(s): Kaku, T., Kawamura, I., Uchiyama, R., Kurenuma, T., Mitsuyama, M.
  • Journal Title: FEMS Microbiol.Lett. 274 Pages: 189-195
  • Peer Reviewed
• [Journal Article] Cytolysindependent escape of the bacterium from the phagosome is required but not sufficient for induction of the Th1 immune response against Listeria monocytogenes infection: distinct role of Listeriolysin O determined by cytolysin gene replacement. (2007)
  • Author(s): Hara, H., Ikuo Kawamura, Nomura, T., Tominaga, T., Tsuchiya, K., Mitsuyama, M.
  • Journal Title: Infect.Immun. 75 Pages: 3791-3801
  • Peer Reviewed
• [Journal Article] Involvement of caspase-9 in the inhibition of necrosis of RAW 264 cells infected with Mycobacterium tuberculosis. (2007)
  • Author(s): Uchiyama, R., Kawamura, I., Fujimura, T., Kawanishi, M., Tsuchiya, K., Tominaga, T., Kaku, T., Fukasawa, Y., Sakai, S., Nomura, T., Mitsuyama, M.
  • Journal Title: Infect.Immun. 75 Pages: 2894-2902
  • Peer Reviewed
• [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A)-mediated adherence enhances the chemokine production by A549 alveolar epithelial cells. (2007)
  • Author(s): Kohiwattanagun, J., Kawamura, I., Fujimura, T., Mitsuyama, M.
  • Journal Title: Microbiol.Immunol. 51 Pages: 253-261
  • NAID: 10020281588
  • Peer Reviewed
• [Journal Article] Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria. (2007)
  • Author(s): Nomura, T., Kawamura, I., Kohda, C., Baba, H., Ito, Y., Kimoto, T., Watanabe, I., Mitsuyama, M.
  • Journal Title: Microbiology 153 Pages: 2250-2258
  • Peer Reviewed
• [Journal Article] Cytolysin-dependent endosomal escape is required but not sufficient for the induction of Th1 immune response against Listeria infection: A distinct role for listeriolysin O as determined by cytolysin gene replacement. (2007)
  • Author(s): Hara H, Kawamura I, Nomura T, Tominaga T & Mitsuyama M.
  • Journal Title: Infect. Immun. 75 Pages: 3791-3801
  • Peer Reviewed
• [Journal Article] RD1 region in mycobacterial genome is involved in induction of necrosis in the infected RAW264 cells via mitochondrial membrane damage and ATP depletion. (2007)
  • Author(s): Kaku T, Kawamura I, Uchiyama R, Kurenuma T & Mitsuyama M
  • Journal Title: FEMS Microbiol. Lett. 274 Pages: 189-195
• [Journal Article] Mycobacterium tuberculosis H37Rv is capable of inhibiting necrosis of the infected macrophages via activation of caspase-9. (2007)
  • Author(s): Uchiyama R, Kawamura I, Kawanishi M, Tsuchiya K, Tominaga T, Kaku T, Fukasawa Y, Sakai S, Fujimura T, Nomura T & Mitsuyama M
  • Journal Title: Infect. Immun. 75 Pages: 2894-2902
• [Journal Article] Mycobacterium tuberculosis H37Rv is capable of inhibiting necrosis of the infected macrophages via activation of caspase-9. (2007)
  • Author(s): Ryosuke Uchiyama, et. al.
  • Journal Title: Infection and Immunity 75 Pages: 2894-2902
  • Peer Reviewed
• [Journal Article] Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria. (2007)
  • Author(s): Nomura Takamasa, et. al.
  • Journal Title: Microbiology 153 Pages: 2250-2258
  • Peer Reviewed
• [Journal Article] Cytolysin-dependent endosomal escape is required but not sufficient for the induction of Thl immune response against Listeria infection: A distinct role for listeriolysin 0 as determined by cytolysin gene replacement. (2007)
  • Author(s): Hideki Hara, et. al.
  • Journal Title: Infection and Immunity 75 Pages: 3791-3801
  • Peer Reviewed
• [Journal Article] RD1 region in mycobacterial genome is involved in induction of necrosis in the infected RAW264 cells via mitochondrial membrane damage and ATP depletion. (2007)
  • Author(s): Taijin Kaku, et. al.
  • Journal Title: FEMS Microbiology Letters 274 Pages: 189-195
  • Peer Reviewed
• [Journal Article] Tuberculosis: Vaccine and drug development (a review) (2007)
  • Author(s): Masao Mitsuyama, et. al.
  • Journal Title: Tuberculosis 87 Pages: 10-13
  • Peer Reviewed
• [Journal Article] Critical involvement of domain 4 of listeriolysin 0 and allied bacterial cytolysins in temperature- and pH-dependent loss of cytolytic activity (2007)
  • Author(s): Takamasa Nomura et al.
  • Journal Title: Microbiology 153(in press)
• [Journal Article] Mycobacterium tuberculosis H37Rv is capable of inhibiting necrosis of the infected macrophages via activation of caspase-9 (2007)
  • Author(s): Ryosuke Uchiyama et al.
  • Journal Title: Infection and Immunity 75(in press)
• [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A) contributes to induction of chemokine and proinflammtory cytokine (2007)
  • Author(s): Juthaporn Kohwiwattanagun et al.
  • Journal Title: Microbiology and Immunology 51(2) Pages: 253-261
• [Journal Article] Dependence of the lethal effect of pore-forming haemolysins of Gram-positive bacteria on cytolytic activity. (2006)
  • Author(s): Watanabe, I., Nomura, T., Tominaga, T., Yamamoto, K., Kohda, C., Kawamura, I., Mitsuyama, M.
  • Journal Title: J.Med.Microbiol. 55 Pages: 505-510
  • Peer Reviewed
• [Journal Article] Listeriolysin O derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice. (2006)
  • Author(s): Yamamoto, K., Kawamura, I., Tominaga, T., Nomura, T., Ito, J., Mitsuyama, M.
  • Journal Title: Clin.Exp.Immunol. 144 Pages: 475-484
  • Peer Reviewed
• [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands: involvement of mast cells and dendritic cells. (2006)
  • Author(s): Yamamoto, K., Kawamura, I., Ito, J., Mitsuyama, M.
  • Journal Title: Clin.Exp.Allergy 36 Pages: 760-769
  • Peer Reviewed
• [Journal Article] Listeriolysin 0 derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic (2006)
  • Author(s): Kazuhiro Yamamoto et al.
  • Journal Title: Clinical and Experimental Immunology 144 Pages: 475-484
• [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands ligands : Involvement of mast cells and dendritic cells (2006)
  • Author(s): Kazuhiro Yamamoto et al.
  • Journal Title: Clinical and Experimental Allergy 36 Pages: 760-769
• [Journal Article] Dependence of the lethal effect of pore-forming cytolysins on the cytolytic activity. (2006)
  • Author(s): Isao Watanabe et al.
  • Journal Title: Journal of Medical Microbiology 55 Pages: 505-510
• [Presentation] 細胞内寄生性細菌の感染戦略と宿主の対応 (2010)
  • Author(s): 光山正雄
  • Organizer: 第42回日本小児感染症学会総会
  • Place of Presentation: 仙台市仙台国際センター
  • Year and Date: 2010-11-27
• [Presentation] 細胞内寄生性細菌の感染戦略と宿主の対応 (2010)
  • Author(s): 光山正雄
  • Organizer: 第42回日本小児感染症学会総会
  • Place of Presentation: 仙台国際センター(仙台市)(招待講演)
  • Year and Date: 2010-11-27
• [Presentation] 結核菌感染マクロファージのinterleukin-1α産生における結核菌病原性関連遺伝子領域RD1の役割 (2010)
  • Author(s): 河村伊久雄、楊瑞麗、陳曦、S.R.Dewamitta、酒井俊介、土屋晃介, ら
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 関西医科大学付属病院講堂(大阪府)
  • Year and Date: 2010-11-20
• [Presentation] Critical role of ASC in the activation of inflammasomes and resistance to pulmonary infection with Streptococcus pneumoniae (2010)
  • Author(s): R.Fang, K.Tsuchiya, I.Kawamura, Y.Shen, H.Hara, et.al.
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 関西医科大学付属病院講堂(大阪府)
  • Year and Date: 2010-11-20
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Hara, H., Tsuchiya, K., Kawamura, I., Yamamoto, T., Nomura, T., Mitsuyama, M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: ICC Kobe, Japan.
  • Year and Date: 2010-08-25
• [Presentation] The importance of Lmo2588/2589 in the ability of Listeria monocytogenes to induce IFN-b production. (2010)
  • Author(s): Yamamoto, T., Hara, H., Tsuchiya, K., Sakai, S., Nomura, T., Kawamura, I., Mitsuyama, M.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: ICC Kobe, Japan.
  • Year and Date: 2010-08-25
• [Presentation] Listeeriolysin O-dependent bacterial entry into cytoplasm is required for calpain activation and IL-1a secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Kawamura, I., Dewamitta, S.R., Nomura, T., Hara, H., Tsuchiya, K., Shen, Y., Daim, S., Yamamoto, T., Qu, H., Sakai, S., Xu, Y., Mitsuyama, M.
  • Organizer: 14^th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-08-25
• [Presentation] PD-1 inhibitory receptor prevents immunopathological responses in murine tuberculosis. (2010)
  • Author(s): Sakai, S., Kawamura, I., Tsuchiya, K., Okazaki, T., Mitsuyama, M.
  • Organizer: 14^th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-08-25
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): H.Hara, K.Tsuchiya, I.Kawamura, T.Yamamoto, et.al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] The importance of Lmo2588/2589 in the ability of Listeria monocytogenes to induce IFN-beta production (2010)
  • Author(s): T.Yamamoto, H.Hara, K.Tsuchiya, S.Sakai, T.Nomura, et.al
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] Listeriolysin 0-dependent bacterial entry into cytoplasm is required for calpain activation and IL-1α secretion in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): I.Kawamura, S.D.Dewamitta, T.Nomura, H.Hara, K.Tsuchiya, Y Shen, S.Daim, et.al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] PD-1 inhibitory receptor prevents immunopathological responses in murine tuberculosis (2010)
  • Author(s): S.Sakai, I.Kawamura, K.Tsuchiya, T.Okazaki, et.al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] a key factor of Listeria monocytogenes in the induction of immune response of the infected host. (2010)
  • Author(s): Mitsuyama M., Listeriolysin O
  • Organizer: International Conference on Veterinary Immunology 2010
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2010-08-17
• [Presentation] Listeriolysin O, a key factor of Listeria monocytogenes in the induction of immune response of the infected host. (2010)
  • Author(s): Masao Mitsuyama
  • Organizer: 9^<th> International Veterinary Immunology Symposium
  • Place of Presentation: タワーホール船堀(東京都)(招待講演)
  • Year and Date: 2010-08-17
• [Presentation] リステリア感染マクロファージにおけるインフラマソーム活性化機構 (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄、山本武司、Daim S., Dewamitta, S.R., 申艶娜、方仁東、光山正雄
  • Organizer: 第21回日本生体防御学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-22
• [Presentation] Listeria monocytogenes感染後のIFN-・産生誘導におけるLmo2589遺伝子の重要性 (2010)
  • Author(s): 山本武司、原英樹、土屋晃介、酒井俊介、野村卓正、Sylvia Daim, ら
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台市戦災復興記念館 (宮城県)
  • Year and Date: 2010-07-22
• [Presentation] リステリア感染マクロファージにおけるインフラマソーム (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄, ら
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台市戦災復興記念館 (宮城県)
  • Year and Date: 2010-07-22
• [Presentation] PD-1 inhibitory signal is required for an orchestrated protective immunity to Mycobacterium tuberculosis in lung (2010)
  • Author(s): S.Sakai, I.Kawamura, M.Mitsuyama
  • Organizer: 45^<th> US-Japan Conference on Tuberculosis and Leprosy
  • Place of Presentation: Broad Institute(Massachusetts, 米国)
  • Year and Date: 2010-07-13
• [Presentation] 細胞内寄生細菌リステリアの病原因子LLOによるサイトカイン誘導機構 (2010)
  • Author(s): 光山正雄
  • Organizer: 第16回日本ヘリコバクター学会学術総会
  • Place of Presentation: 芝蘭会館(京都市)(招待講演)
  • Year and Date: 2010-06-24
• [Presentation] Listeria monocytogenes感染後のIFN-β産生誘導における薬剤排出ポンプ類似タンパク質Lmo2588の重要性 (2010)
  • Author(s): 山本武司、原英樹、河村伊久雄、光山正雄
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2010-04-05
• [Presentation] Important role of ASC in resistance to Mycobacterium tuberculosis infection in vivo. (2010)
  • Author(s): Dewamitta S.R.Sakai, S.Tsuchiya, K.Hara, H.Nomura, T.Kawamura, I., Mitsuyama, M.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] PD-1 inhibitory receptor prevents immunopathological responses in pulmonary tuberculosis (2010)
  • Author(s): ○酒井俊祐, 河村伊久雄, 土屋晃介, 岡崎拓, 本庶佑, 光山正雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2010-03-28
• [Presentation] Mechanism of inflammasome activation upon infection with Listeria monocytogenes. (2010)
  • Author(s): Tsuchiya K., Hara, H.Nomura, T.Daim, S.Dewamitta, S.R.Kawamura, I.Mitsuyama, M.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] contributes to inflammasome formation through the phosphorylation of Syk. (2010)
  • Author(s): Hara H., Tsuchiya K., Yamamoto T., Kawamura I., Nomura T., Mitsuyama M.Listeriolysin O
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] Mechanism of inflammasome activation upon infection with Listeria monocytogenes (2010)
  • Author(s): ○土屋晃介, 原英樹, 野村卓正, SylviaDaim, Sita Dewamitta, 河村伊久雄, 光山正雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2010-03-27
• [Presentation] Sykのリン酸化を介したlisteriolysin Oのインフラマソーム形成への関与 (2010)
  • Author(s): ○原英樹, 土屋晃介, 山本武司, 河村伊久雄, 野村卓正, 光山正雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] Listeria monocytogenes菌株間でのIFNβ産生誘導能の違いにおけるImo2589の重要性 (2010)
  • Author(s): ○山本武司, 原英樹, 土屋晃介, 酒井俊祐, 野村卓正, Sylvia Daim, 河村伊久雄, 光山正雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] Important role of ASC in resistance to Mycobacterium tuberculosis infection in vivo (2010)
  • Author(s): 〇Sita Dewamitta, 酒井俊祐, 土屋晃介, 原英樹, 野村卓正, 河村伊久雄, 光山正雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] TLR2-dependent signal is involved in the efficient phagocytosis of Listeria monocytogenes by macrophages. (2009)
  • Author(s): Shen Y., Nomura T., Tsuchiya K., Hara H., Sakai S., Qu H., Daim S., Dewamitta, S.R., Yamamoto T., Kawamura I., Mitsuyama M.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] LLO participates in caspase-1 activation in Listeria monocytogenes-infected macrophages via induction of IFN-b production. (2009)
  • Author(s): Hara H., Tsuchiya K., Kawamura I., Yamamoto T., Nomura T., Mitsuyama M.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Bacterial cytoplasmic entry -inducing intracellular calcium signaling is required for IL-1a/b production in Listeria monocytogenes-infected macrophages. (2009)
  • Author(s): Nomura T., Dewamitta S.R., Hara H., Tsuchiya K., Kawamura I., Mitsuyama M.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Mechanisme fo the inflammasome activation upon infection with Listeria monocytogenes (2009)
  • Author(s): TSUCHIYA Kohsuke, HARA Hideki, NOMURA Takamasa,DEWAMITTA Sita, DAIM Sylvia, KAWAMURA Ikuo, MITSUYAMA Masao
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] LLO participates in caspase-1 activation in Listeria monocytogenes-infected macrophage via induction of IFN-β production (2009)
  • Author(s): HARA Hideki, TSUCHIYA Kohsuke, KAWAMURA Ikuo, YAMAMOTO Takeshi, NOMURA Takamasa, MITSUYAMA Masao
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] RD 1 locus in Mycobacterium tuberculosis genome contributes to the activation of caspase-1 via induction of potassium efflux in infected macrophages (2009)
  • Author(s): Sita R.Dewamitta, Ikuo Kawamura, Hideki Hara, Ryosuke Uchiyama, Sylvia Daim, Shunsuke Sakai, Kohsuke Tsuchiya, Takamasa Nomura, Masao Mitsuvama
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] Bacterial cytoplasimic entry-inducing intracellular calcium signaling is required for IL-1α/β production in Listeria monocytogenes-infected macrophages (2009)
  • Author(s): NOMURA Takamasa, DEWAMITTA R.Sita, HARA Hideki, TSUCHIYA Kohsuke, KAWAMURA Ikuo, MITSUYAMA Masao
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] TLR2-dependent signal is involved in the efficient phagocytosis of Listeria monocytogenes by macrophages (2009)
  • Author(s): SHEN Yanna, NOMURA Takamasa, TSUCHIYA Kohsuke, HARA Hideki, SAKAI Shunsuke, QU Huixin, DAIM Sylvia, DEWAMITTA Sita R., YAMAMOTO Takeshi, KAWAMURA Ikuo, MITSUYAMA Masao
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] The PD-1 : PD-L co-inhibitory pathway is a critical determinant of host resistance to pulmonary tuberculosis (2009)
  • Author(s): SAKAI Shunsuke, KAWAMURA Ikuo, TSUCHIYA Kohsuke, OKAZAKI Taku, MITSUYAMA Masao
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-04
• [Presentation] α-GalCerはMφのファゴソーム内でリステリアの殺菌能を高進する (2009)
  • Author(s): WATANABE Kazuki, SHIMIZU Takamitsu, EMOTO Yoshiko, KAWAMURA Ikuo, MITSUYAMA Masao, EMOTO Masashi
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(大阪市)
  • Year and Date: 2009-12-03
• [Presentation] PD-1-dependent signal impairs protective T cell response in chronic Mycobacterium bovis BCG infection in mice. (2009)
  • Author(s): Kawamura I., Sakai S., Mitsuyama M.
  • Organizer: The 45^th Tuberculosis and Leprosy Research Conference.
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-29
• [Presentation] LLO-dependent entry of Listeria monocytogenes into cytoplasm is essential for calcium dependent calpain activation and IL-1a secretion in infected macrophages. (2009)
  • Author(s): Dewamitta S.R., 野村卓正、原英樹、土屋晃介、暮沼武士、申艶娜、Daim S., 酒井俊祐、屈慧新、山本武司、河村伊久雄、光山正雄
  • Organizer: 第20回日本生体防御学会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-25
• [Presentation] The PD-1:PD-L1 pathway is involved in the persistent infection with Mycobacterium bovis BCG. (2009)
  • Author(s): 酒井俊祐、河村伊久雄、内山良介、光山正雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-13
• [Presentation] Mycobacterium tuberculosis PPE37protein alters the early cytokine response of macrophages. (2009)
  • Author(s): Sylvia Daim、河村伊久雄、原英樹、申艶娜、暮沼武士、光山正雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-13
• [Presentation] 細胞内寄生性リステリア病原因子による宿主免疫応答の誘導機構-生菌免疫の作用機序へのアプローチ- (2009)
  • Author(s): 光山正雄
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 細胞内寄生性リステリア病原因子による宿主免疫応答の誘導機構-生菌免疫の作用機序へのアプローチ- (2009)
  • Author(s): 光山正雄
  • Organizer: 第82回日本細菌学会浅川賞受賞講演
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] Possible role of PPE37 protein in Mycobacterium tuberculosis pathogenesis. (2008)
  • Author(s): Daim S., Kawamura I., Kurenuma T., Mitsuyama M.
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] The PD-1:PD-L1 pathway inhibits the protective immunity and contributes to the bacterial persistence in mycobacterial infection. (2008)
  • Author(s): Sakai S., Kawamura I., Uchiyama R., Okazaki T., Mitsuyama M.
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] The interaction between Listeria monocytogenes virulence factors and the host macrophages The 9^th Korea-Japan International Symposium ofMicrobiology (2008)
  • Author(s): Mitsuyama M.
  • Place of Presentation: Seoul, Korea.
  • Year and Date: 2008-10-17
• [Presentation] Mycobacterium tuberculosis, Listeria monocytogenes and the host cytokine response. (2008)
  • Author(s): Masao Mitsuyama
  • Organizer: 第11回京都大学・復旦大学国際シンポジウム
  • Place of Presentation: 上海、中国
  • Year and Date: 2008-10-11
• [Presentation] Mycobacterium tuberculosis, Listeria monocytogenes and the host cytokine response (2008)
  • Author(s): Masao Mitsuyama
  • Organizer: 第11回京都大学・復旦大学国際シンポジウム
  • Place of Presentation: 上海、中国
  • Year and Date: 2008-10-11
• [Presentation] Critical involvement of bacterial virulence factor in the host immune response to Listeria monocytogenes International Union of Microbiological Societies 2008 (2008)
  • Author(s): Mitsuyama M.
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2008-08-08
• [Presentation] Critical involvement of bacterial virulence factor in the host immune response to Listeria monocytogenes. (2008)
  • Author(s): Masao Mitsuyama
  • Organizer: 国際微生物学会議2008 (IUMS2008)
  • Place of Presentation: イスタンブール、トルコ
  • Year and Date: 2008-08-08
• [Presentation] Critical involvement of bacterial virulence factor in the host immune response to Listeria monocytogenes (2008)
  • Author(s): Masao Mitsuyama
  • Organizer: Plenary lecture、国際微生物学会議2008(IUMS2008)
  • Place of Presentation: イスタンブール、トルコ
  • Year and Date: 2008-08-08
• [Presentation] 結核菌およびBCGの持続感染における免疫抑制受容体PD-1の役割 (2008)
  • Author(s): 酒井俊祐、河村伊久雄、内山良介、光山正雄
  • Organizer: 第19回日本生体防御学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-12
• [Presentation] RD1 region in mycobacterial genome is involved in the induction of necrosis in infected macrophages via mitochondrial membrane damage and ATP depletion. (2008)
  • Author(s): Kawamura I., Kaku T., Uchiyama R., Kurenuma T., Mitsuyama M.
  • Organizer: US-Japan Cooperative Medical Science Program The 43rd Tuberculosis and Leprosy Research Conference.
  • Place of Presentation: Baltimore, MD, USA.
  • Year and Date: 2008-07-08
• [Presentation] 結核菌病原遺伝子領域RD1が関与するIFN-g産生誘導機序の解析 (2008)
  • Author(s): 暮沼武士、内山良介、河村伊久雄、光山正雄
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-26
• [Presentation] The role of virulence factor of Listeria monocytogenes in the host response (2008)
  • Author(s): Masao Mitsuyama
  • Organizer: 81st Annual Congress of Bacteriology (Symposium 1)
  • Place of Presentation: 京都市 国立京都国際会議場
  • Year and Date: 2008-03-24
• [Presentation] Involvement of caspase-9 in the inhibition of necrosis of peritoneal macrophages infected with Mycobacterium tuberculosis. (2007)
  • Author(s): Uchiyama R., Kawamura I., Tsuchiya K., Tominaga T., Sakai S., Nomura T., Mitsuyama M.
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] Mechanism in the requirement of listeriolysin O (LLO) for the induction of caspase-dependent cytokines in macrophages infected with Listeria monocytogenes. (2007)
  • Author(s): Masao Mitsuyama
  • Organizer: 第13回国際免疫学会シンポジウム
  • Place of Presentation: リオデジャネイロ、ブラジル
  • Year and Date: 2007-08-25
• [Presentation] Mechanism in the requirement of listeriolysin 0 (LL0) for the induction of caspase-dependent cytokines in macrophages infected with Listeria (2007)
  • Author(s): Masao Mitsuyama
  • Organizer: 13th International Congress of Immunology (Symposium 73)
  • Place of Presentation: ブラジル国リオデジャネイロ 国際会議場
  • Year and Date: 2007-08-25
• [Presentation] Mechanism in the requirement of listeriolysin O for the induction of caspase-dependent cytokines in macrophages infectd with Listeria monocytogenes. (2007)
  • Author(s): Mitsuyama M.
  • Organizer: The 13^th International Congress of Immunology
  • Place of Presentation: Rio de Janeiro, Brazil.
  • Year and Date: 2007-07-28
• [Presentation] Interaction of intracellular parasitic bacteria and host defense system for the generation of Th1-dependent acquired immunity (2007)
  • Author(s): Masao Mitsuyama
  • Organizer: COE International Symposium 2007 in Kyoto
  • Place of Presentation: 京都市 京都大学芝蘭会館
  • Year and Date: 2007-06-02
• [Presentation] Mycobacterium tuberculosis H37Rv but not H37Ra induces generation of intracellular reactive oxygen species and replicates in macrophage-like RAW 264 cells. (2006)
  • Author(s): Kawamura I., Uchiyama R., Mitsuyama M.
  • Organizer: The 41th US-Japan conference on tuberculosis and leprosy.
  • Place of Presentation: Kagoshima, Japan.
  • Year and Date: 2006-07-19
• [Presentation] リステリア感染マクロファージにおけるサイトカイン応答の多様性 (2006)
  • Author(s): 光山正雄
  • Organizer: 第79回日本細菌学会総会シンポジウム S4 : 細菌と宿主の攻防
  • Place of Presentation: 金沢市
  • Year and Date: 2006-03-30
• [Book] 細菌と感染現代生物科学入門第5巻免疫o感染生物学 (2011)
  • Author(s): 光山正雄
  • Publisher: 岩波書店 東京
• [Book] 現代生物科学入門 5.免疫感染生物学 (分担:細菌と感染) (2011)
  • Author(s): 光山正雄
  • Total Pages: 240
  • Publisher: 岩波書店
• [Book] 感染症-ウイルスo細菌o寄生虫の感染戦略 (2009)
  • Author(s): 光山正雄(編集、分担執筆)
  • Publisher: 羊土社 東京
• [Book] 感染症-ウイルス・細菌・寄生虫の感染戦略実験医学増刊号 編集:光山正雄、北潔、野本明男 (2009)
  • Author(s): 光山正雄(編集、分担執筆)
  • Total Pages: 240
  • Publisher: 羊土社
• [Book] Adaptive immunity to Listeria monocytogenes(Liu Don, eds.)(Handbook of Listeria monocytogenes)chapter 14, pp.427-448 New York (2008)
  • Author(s): Masao Mitsuyama
  • Publisher: CRC Press
• [Book] Adaptive Immunity to Listeria monocytogenes. Chapter 14 (pp427-448) In Handbook of Listeria monocytogenes Ed by Dongyou Liu Total (2008)
  • Author(s): Masao Mitsuyama
  • Total Pages: 540
  • Publisher: CRC Press New York
• [Book] Adaptive Immunity to Listeria monocytogenes. Chapter 14(pp427-448), In Handbook of Listeria monocytogenes Ed by Dongyou Liu (2008)
  • Author(s): Masao Mitsuyama
  • Total Pages: 540
  • Publisher: CRC Press New York
• [Book] Listeria monocytogenes Handbook (2008)
  • Author(s): Masao Mitsuyama
  • Total Pages: 552
  • Publisher: Taylor & Francis, CRC Press
• [Book] 生体防御医学事典(鈴木和男監修, 光山正雄他編著) (2007)
  • Author(s): 光山正雄(編集、分担執筆)
  • Total Pages: 362
  • Publisher: 朝倉書店
• [Book] 生体防御医学事典 (鈴木和男監修, 光山正雄他編著) (2007)
  • Author(s): 光山正雄
  • Total Pages: 365
  • Publisher: 朝倉書店
• [Book] メルクマニュアル第18版 (2006)
  • Author(s): 光山正雄(監訳)
  • Publisher: 日経BP社
• [Book] 結核(泉孝英監修 冨岡洋海編集 改訂第4版)第1章A結核菌の基礎 (2006)
  • Author(s): 光山正雄
  • Total Pages: 458
  • Publisher: 医学書院
• [Book] 結核(改訂第4版)(泉孝英監修, 冨岡洋海編集) (2006)
  • Author(s): 光山正雄(分担執筆)
  • Total Pages: 458
  • Publisher: 医学書院
• [Remarks] ホームページ等
  • URL: http://bisei15.mb.med.kyoto-u.ac.jp/index.html