Single-molecule study on rotary catalysis of FoF1-ATP synthase
Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
Project/Area Number |
18074005
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Osaka University |
Principal Investigator |
NOJI Hiroyuki Osaka University, 産業科学研究所, 招へい教授 (00343111)
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Co-Investigator(Kenkyū-buntansha) |
TABATA Kazuhito 東京大学, 工学系研究科, 助教 (50403001)
IINO Ryota 東京大学, 工学系研究科, 講師 (70403003)
井出 徹 大阪大学, 生命機能研究科, 准教授 (60231148)
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Co-Investigator(Renkei-kenkyūsha) |
IDE Toru 大阪大学, 生命機能研究科, 准教授 (60231148)
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Project Period (FY) |
2006 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥225,700,000 (Direct Cost: ¥225,700,000)
Fiscal Year 2010: ¥34,400,000 (Direct Cost: ¥34,400,000)
Fiscal Year 2009: ¥45,300,000 (Direct Cost: ¥45,300,000)
Fiscal Year 2008: ¥49,900,000 (Direct Cost: ¥49,900,000)
Fiscal Year 2007: ¥49,800,000 (Direct Cost: ¥49,800,000)
Fiscal Year 2006: ¥46,300,000 (Direct Cost: ¥46,300,000)
|
Keywords | 1分子計測 / 分子モーター / 生物物理 / 回転分子モーター / 生体膜 / ナノバイオ / エネルギー変換 / MEMS / 分子シミュレーション / 1 分子計測 / ナノバイオロジー / ナノバイオテクノロジー / ATP合成酵素 / べん毛モーター / マイクロ・ |
Research Abstract |
We developed a wide variety of nanobiotechnogoly, taking the advantageous features of this interdisciplinary research project. Included are single-molecule imaging method integrated with planner bilayer system, novel microdevices for single-molecule detection, novel fluorescent probe, and novel torque-determination method based on fluctuation theorem. In addition, we revealed many fundamental insights about the mehcano-chemical coupling mechanism of F_oF_1-ATPase, such as the establishment of reaction scheme of F_1, mechanical modulation of reaction equilibrium, and structural basis of cooperativity among 3 torque-generating beta subunits.
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Report
(7 results)
Research Products
(181 results)
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[Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009
Author(s)
Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji H.
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Journal Title
EMBO J. 28
Pages: 3279-3289
Related Report
Peer Reviewed
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[Journal Article] Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-bas ed genetically encoded indicators2009
Author(s)
Imamura, H., Huynh Nhat, K.P., Togawa, H., Saito, K., Iino, R., Kato-Yamada, Y., Nagai, T., Noji H.
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Journal Title
Proc Natl Acad Sci USA. 106
Pages: 15651-15656
Related Report
Peer Reviewed
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[Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009
Author(s)
Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji, H.
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Journal Title
EMBO J. 28
Pages: 3279-3289
Related Report
Peer Reviewed
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[Journal Article] Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators2009
Author(s)
Imamura, H., Huynh Nhat, K.P., Togawa, H., Saito, K., Iino, R., Kato-Yamada, Y., Nagai, T., Noji, H.
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Journal Title
Proc Natl Acad Sci USA. 106
Pages: 15651-15656
Related Report
Peer Reviewed
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[Journal Article] Photo gel-sol/sol-gel transition and its patterning of a supramolecular hydrogel as stimuli-responsive biomaterials2008
Author(s)
Matsumoto S, Yamaguchi S, Ueno S, Komatsu H, Ikeda M, Ishizuka K, Iko Y, Tabata KV, Aoki H, Ito S, Noji H, Hamachi I
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Journal Title
Chemistry 14(13)
Pages: 3977-3986
Related Report
Peer Reviewed
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[Journal Article] Photo gel-sol/sol-gel transition and its patterning of a supramolecular hydrogel as stimuli-responsive biomaterials2008
Author(s)
Matsumoto S, Yamaguchi S, Ueno S, Komatsu H, Ikeda M, Ishizuka K, Iko Y, Tabata KV, Aoki H, Ito S, Noji H, Hamachi I.
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Journal Title
Chemistry 14(13)
Pages: 3977-3986
Related Report
Peer Reviewed
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[Journal Article] Visualization of RecA filaments and DNA by fluorescence microscopy2007
Author(s)
Nishinaka, T., Doi Y., Hashimoto, M., Hara, R., Shibata, T., Harada, Y., Kinosita, K.Jr, Noji, H., Yashima, E.
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Journal Title
Journal of Biochemistry (In press)
NAID
Related Report
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[Journal Article] Single Molecule Energetics of F1-ATPase motor2007
Author(s)
Muneyuki, E., Watanabe-Nakayama, T., Suzuki, T., Yoshida, M., Nishizaka, T., Noji, H
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Journal Title
Biophysical Journal (In press)
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[Presentation] 大腸菌内のFoF1-ATP合成酵素の回転速度の1分子計測2010
Author(s)
大坂拓也, 飯野亮太, Karin Seyfert, Michael Borsch, 野地博行
Organizer
第36回生体エネルギー研究会&特定領域「膜超分子モーターの革新的ナノサイエンス」終了シンポジウム
Place of Presentation
大阪大学銀杏会館(大阪)
Year and Date
2010-11-20
Related Report
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