| Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
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| Project/Area Number |
18074005
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
NOJI Hiroyuki Osaka University, 産業科学研究所, 招へい教授 (00343111)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Kazuhito 東京大学, 工学系研究科, 助教 (50403001)
IINO Ryota 東京大学, 工学系研究科, 講師 (70403003)
井出 徹 大阪大学, 生命機能研究科, 准教授 (60231148)
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| Co-Investigator(Renkei-kenkyūsha) |
IDE Toru 大阪大学, 生命機能研究科, 准教授 (60231148)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥225,700,000 (Direct Cost: ¥225,700,000)
Fiscal Year 2010: ¥34,400,000 (Direct Cost: ¥34,400,000)
Fiscal Year 2009: ¥45,300,000 (Direct Cost: ¥45,300,000)
Fiscal Year 2008: ¥49,900,000 (Direct Cost: ¥49,900,000)
Fiscal Year 2007: ¥49,800,000 (Direct Cost: ¥49,800,000)
Fiscal Year 2006: ¥46,300,000 (Direct Cost: ¥46,300,000)
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| Keywords | 1分子計測 / 分子モーター / 生物物理 / 回転分子モーター / 生体膜 / ナノバイオ / エネルギー変換 / MEMS / 分子シミュレーション / 1 分子計測 / ナノバイオロジー / ナノバイオテクノロジー / ATP合成酵素 / べん毛モーター / マイクロ・ |
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| Research Abstract |
We developed a wide variety of nanobiotechnogoly, taking the advantageous features of this interdisciplinary research project. Included are single-molecule imaging method integrated with planner bilayer system, novel microdevices for single-molecule detection, novel fluorescent probe, and novel torque-determination method based on fluctuation theorem. In addition, we revealed many fundamental insights about the mehcano-chemical coupling mechanism of F_oF_1-ATPase, such as the establishment of reaction scheme of F_1, mechanical modulation of reaction equilibrium, and structural basis of cooperativity among 3 torque-generating beta subunits.
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