Mechanics of supermolecular motor proteins revealed by an advanced optical microscope designed to track three dimensional dynamics.
| Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
|---|
| Project/Area Number |
18074008
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Gakushuin University |
|---|
Principal Investigator |
NISHIZAKA Takayuki Gakushuin University, 理学部, 教授 (40359112)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MASAIKE Tomoko 学習院大学, 理学部, 助教 (60406882)
矢島 潤一郎 学習院大学, 理学部, 研究員 (00453499)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YAJIMA Junichiro 学習院大学
ADACHI Kengo 学習院大学
SUGAWA Mitsuhiro 学習院大学
|
|---|
| Research Collaborator |
HORIBE Keiko 学習院大学
TATSUGUCHI Ayako 学習院大学
|
|---|
| Project Period (FY) |
2006 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥112,400,000 (Direct Cost: ¥112,400,000)
Fiscal Year 2010: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2009: ¥23,300,000 (Direct Cost: ¥23,300,000)
Fiscal Year 2008: ¥24,300,000 (Direct Cost: ¥24,300,000)
Fiscal Year 2007: ¥24,300,000 (Direct Cost: ¥24,300,000)
Fiscal Year 2006: ¥24,300,000 (Direct Cost: ¥24,300,000)
|
|---|
| Keywords | 1分子生物物理学 / 光学顕微鏡 / 分子モーター / タンパク質科学 / 顕微操作 / 全反射型顕微鏡 / 光ピンセット / 3次元検出 / F1-ATPase / タンパク質構造 / 1分子ナノ計測 / バイオイメージング / 1分子計測・操作 / 構造生物学 / タンパク質 / F_1ATPase / 蛋白質の構造 |
|---|
| Research Abstract |
The final goal of our group is to establish the missing link between chemical reaction and mechanical event in molecular motors. To achieve this, we have developed advanced versions of conventional optical microscopes and applied them into single-molecule techniques. Our main contributions are following two studies: one is about the kinesin-microtubule system and the other F1-ATPase. These techniques are applicable to other molecular machines, hopefully in more sophisticated ways, and we hope to investigate this in future studies as a new research field 'single-molecule structural biology.'
|
Report
(7 results)
Research Products
(126 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation2007
Author(s)
Adachi, K., Oiwa, K., Nishizaka, T., Furuike, S., Noji, H., Itoh, H., Yoshida, M., and Kinosita, K., Jr.
-
Journal Title
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Versatility of the unbinding force measurements at the single molecule level adapted to different molecular motors2009
Author(s)
Sergey Mikhailenko, Oguchi, Y., Ohki, T., Olivares, A.O., De La Cruz, E.M., Ishiwata, S., Nishizaka, T.
Organizer
日本生物物群会第47回年会
Place of Presentation
アスティとくしま
Year and Date
2009-10-30
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
