| Budget Amount *help |
¥70,200,000 (Direct Cost: ¥70,200,000)
Fiscal Year 2010: ¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 2009: ¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2008: ¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2007: ¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2006: ¥15,300,000 (Direct Cost: ¥15,300,000)
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| Research Abstract |
Cytoplasmic male sterility (CMS) has been reported in more than 140 angiosperm species. Genes responsible for CMS (S gene) are encoded in mitochondrial genomes, while plants with S gene are male fertile when the plants have nuclear genes termed restorer of fertility (Rf). Molecular mechanism of the interaction between S and Rf genes was investigated in this study using sugar beet. Protein-protein interaction was detected between preSatp6, an S gene, and Rf1. This interaction was observed only in anthers in which Rf1 was expressed. This study identified nuclear genes that were suppressed in CMS anthers. It was inferred that deleterious mutation could be inherited through pollen. This possibility was investigated using Arabidopsis mutant with defect in the genes encoding RNA polymerase. This mutation was confirmed to be transmitted through pollen. Spinach is a monoecious species with male heterogamety system. However, dioecious or hermaphroditic spinach emerges when an M gene exists. This study revealed that male determining gene and the M gene located on the same linkage group but resided in different genetic loci.
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