| Budget Amount *help |
¥88,300,000 (Direct Cost: ¥88,300,000)
Fiscal Year 2010: ¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2009: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2008: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2007: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2006: ¥19,200,000 (Direct Cost: ¥19,200,000)
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| Research Abstract |
Here I aimed to study the genome barrier in pollen tube guidance and double fertilization, by using the in vitro Torenia and Arabidopsis systems and our many micromanipulation techniques. I focused on pollen tube attractants derived from the synergid cell, an ovular factor AMOR that makes pollen tubes growing through the cut style competent to be attracted to the embryo sac, and dynamics of two sperm cells and molecular mechanism for double fertilization. I discovered attractant peptide LUREs that was derived from the synergid cell, which had been searched for more than 140 years. LUREs showed rapid and drastic molecular evolution consistent with that these were critical factors for reproductive isolation. AMOR was shown to be related to sugar chains specific to ovule tissues and species. Double fertilization was directly observed for the first time in the living material, whereby we could contribute to advances of this field. Visualization of double fertilization was also useful to get various mutants defective in double fertilization, which would be a significant basis for further study of the molecular mechanism of double fertilization.
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