Key molecules for the genome barrier of pollen tube guidance and double fertilization
| Project Area | Genome Barriers in Plant Reproduction |
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| Project/Area Number |
18075004
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University (2007-2010)
The University of Tokyo (2006) |
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Principal Investigator |
HIGASHIYAMA Tetsuya Nagoya University, 大学院・理学研究科, 教授 (00313205)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥88,300,000 (Direct Cost: ¥88,300,000)
Fiscal Year 2010: ¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2009: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2008: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2007: ¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2006: ¥19,200,000 (Direct Cost: ¥19,200,000)
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| Keywords | 植物 / 花粉管ガイダンス / 重複受精 / 細胞間シグナリング / 顕微細胞操作 / 花粉管 / ガイダンス / 誘引物質 / 助細胞 / ライブイメージング / 受精能獲得 / ゲノム障壁 |
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| Research Abstract |
Here I aimed to study the genome barrier in pollen tube guidance and double fertilization, by using the in vitro Torenia and Arabidopsis systems and our many micromanipulation techniques. I focused on pollen tube attractants derived from the synergid cell, an ovular factor AMOR that makes pollen tubes growing through the cut style competent to be attracted to the embryo sac, and dynamics of two sperm cells and molecular mechanism for double fertilization. I discovered attractant peptide LUREs that was derived from the synergid cell, which had been searched for more than 140 years. LUREs showed rapid and drastic molecular evolution consistent with that these were critical factors for reproductive isolation. AMOR was shown to be related to sugar chains specific to ovule tissues and species. Double fertilization was directly observed for the first time in the living material, whereby we could contribute to advances of this field. Visualization of double fertilization was also useful to get various mutants defective in double fertilization, which would be a significant basis for further study of the molecular mechanism of double fertilization.
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Report
(7 results)
Research Products
(162 results)
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[Journal Article] Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers.2006
Author(s)
Nishimura, Y., Yoshinari, T., Naruse, K., Yamada, T., Sumi, K., Mitani, H., Higashiyama, T., Kuroiwa T.
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Journal Title
Proc. Natl. Acad. Sci. USA 103
Pages: 1382-1387
Related Report
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[Journal Article] Species specificity of the pollen tube attractant derived from the synergid cell of Torenia fournieri.2006
Author(s)
Higashiyama, T., Inatsugi, R., Sakamoto, S., Sasaki, N., Mori, T., Kuroiwa, H., Nakada, T., Nozaki, H., Kuroiwa, T., Nakano, A.
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Journal Title
Plant Physiol. 142
Pages: 481-491
Related Report
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