Elucidation of Regulatory Networks of Embryo Maturation

• Project Area: Genome Barriers in Plant Reproduction
• Project/Area Number: 18075007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: HATTORI Tsukaho 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Shin 名古屋大学, 生物機能開発利用研究センター, 准教授 (00432253) ; KAGAYA Yasuaki 三重大学, 生命科学研究支援センター, 助教 (20335152)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥83,000,000 (Direct Cost: ¥83,000,000); Fiscal Year 2010: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2009: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2008: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2007: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2006: ¥16,600,000 (Direct Cost: ¥16,600,000)
• Keywords: 種子形成 / シロイヌナズナ / イネ / 染色体 / 転写因子 / 胚 / 胚乳 / 発芽 / エピジェネティック制御 / 種子 / 蛍光タンパク質 / 遺伝子 / 休眠 / オーキシン / アブシジン酸

Research Abstract

Inter-specific/generic crosses in higher plants often fail to produce germinatable seeds. This means the presence of genome barriers in seed development. Thus, we explored regulatory mechanisms of seed formation in various aspects. Through these studies, we identified and characterized ENLI gene, which was found essential exceptionally to rice (or cereal) endosperm development at early stages. We also revealed the involvement of epigenetic mechanisms in both positive and negative seed-specific gene regulations.

Research Products

• [Journal Article] Ectopic gene expression and organogenesis in Arabidopsis mutants missing Brul required for genome maintenance (2011)
  • Author(s): Ohno, Y., Jarunya Narangajavana, J., Yamamoto, A., Hattori, T., Kagaya Y., Paszkowski, J., Gruissem, W., Hennig, L. and Takeda, S.
  • Journal Title: Genetics Volume: 189 Pages: 83-95
  • Peer Reviewed
• [Journal Article] Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance (2011)
  • Author(s): Ohno, Y., Narangajavana, J., Yamamoto, A., Hattori, T., Kagaya, Y., Paszkowski, J., Gruissem, W., Hennig, L., Takeda, S.
  • Journal Title: Genetics Volume: 189 Pages: 83-95
  • Peer Reviewed
• [Journal Article] Molecular cloning of Sdr4, a regulator involved in seed dormancy and domestication of rice (2010)
  • Author(s): Sugimoto, K., Takeuchi, Y., Ebana, K., Miyao, A., Hirochika, H., Hara, N., Ishiyama, K., Kobayashi, M., Ban, Y., Hattori, T. and Yano, M.
  • Journal Title: Proc. Natl. Acad. Sci . USA. Volume: 107 Pages: 5792-5797
  • Peer Reviewed
• [Journal Article] Diverse roles and mechanisms of gene regulation by the Arabidopsis seed maturation master regulator FUS3 revealed by microarray analysis (2010)
  • Author(s): Yamamoto, A., Kagaya Y., Usui, H., Hobo, T, Takeda, S. and Hattori, T.
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 2031-2046
  • NAID: 10028056459
  • Peer Reviewed
• [Journal Article] Diverse roles and mechanisms of gene regulation by the Arabidopsis seed maturation master regulator FUS3 revealed by microarray analysis (2010)
  • Author(s): Yamamoto, A., Kagaya, Y., Usui, H., Hobo, T, Takeda, S., Hattori, T.
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 2031-2046
  • NAID: 10028056459
  • Peer Reviewed
• [Journal Article] 解明が進むアブシジン酸受容体とシグナル伝達メカニズム (2010)
  • Author(s): 西村宣之、服部束穂
  • Journal Title: 植物の生長調節 Volume: 45 Pages: 140-143
• [Journal Article] Arabidopsis NF-YR subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors (2009)
  • Author(s): Yamamoto, A., Kagaya, Y., Toyoshima, R., Kagaya, M., Takeda, S. and Hattori, T.
  • Journal Title: Plant J. Volume: 58 Pages: 843-856
  • Peer Reviewed
• [Journal Article] Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner (2009)
  • Author(s): Kagaya, Y. and Hattori, T.
  • Journal Title: Genes Genet Syst. Volume: 84 Pages: 95-99
  • NAID: 10024791528
• [Journal Article] Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors. (2009)
  • Author(s): Yamamoto A, Kagaya Y, Toyoshima R, Kagaya M, Takeda S, Hattori T
  • Journal Title: The Plant Journal 58(5) Pages: 843-856
  • Peer Reviewed
• [Journal Article] Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner. (2009)
  • Author(s): Kagaya, Y. and Hattori, T.
  • Journal Title: Genes Genet Syst. 84 Pages: 95-99
  • NAID: 10024791528
  • Peer Reviewed
• [Journal Article] Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE- binding factors. (2009)
  • Author(s): Yamamoto, A., Kagaya, Y., Toyoshima, R., Kagaya, M., Takeda, S. and Hattori, T.
  • Journal Title: Plant J.
  • Peer Reviewed
• [Journal Article] Arabidopsis NF-YB subunits LEC 1 and LEC 1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors (2009)
  • Author(s): Yamamoto, A., Kagaya, Y., Toyoshima, R., Kagaya, M., Takeda, S. and Hattori, T.
  • Journal Title: Plant J. on-line
  • Peer Reviewed
• [Journal Article] rabidopsis transcription factors, RAV 1 and RAV 2, are regulatedby touch-related stimuli in a dose-dependent and biphasic manner (2009)
  • Author(s): Kagaya, Y. and Hattori, T.
  • Journal Title: Genes Genet Syst. 84 Pages: 95-99
  • Peer Reviewed
• [Journal Article] アブシジン酸受容体. (2008)
  • Author(s): 服部束穂、伴佳典、加賀谷安章、武田真
  • Journal Title: 植物の生長調節 43 Pages: 123-134
• [Journal Article] アブシジン酸受容体 (2008)
  • Author(s): 服部束穂、伴佳典、加賀谷安章、武田真
  • Journal Title: 植物の生長調節 43 Pages: 123-134
• [Journal Article] Physical span size of the S locus region defined by genetic recombination and genome sequences in Tpomoea trifida, Convolvulaceae (2007)
  • Author(s): Rahman, Md H. , Tsuchiya, T. , Suwabe, K., Kohori, J., Tomita, R. N. , Kagaya, Y., Kobayashii, I., Kakeda, K. and Kowyama, Y.
  • Journal Title: Plant Sexual Reproduction Volume: 20 Pages: 73-85
  • Peer Reviewed
• [Journal Article] Expression of stigma- and anther-specific genes located in the S locus region of Ipomoea trifida (2007)
  • Author(s): Rahman, Md H., Uchiyama, M., Kuno, M., Hirashima, N., Suwabe, K., Tsuchiya, T., Kagaya, Y., Kobayashi, I. , Kakeda, K. and Kowyama, Y.
  • Journal Title: Plant Sexual Reproduction Volume: 20 Pages: 63-72
  • Peer Reviewed
• [Journal Article] 高浸透圧ストレスおよびアブシジン酸のシグナル伝達に関与するプロテインキナーゼ-SnRK2プロテインキナーゼを中心として. (2007)
  • Author(s): 服部束穂, 小林裕子
  • Journal Title: 蛋白質核酸酵素 増刊 52・6 Pages: 557-564
• [Journal Article] Physical span size of the S locus region defined by genetic recombination and genome sequences in Ipomoea trifida, Convolvulaceae. (2007)
  • Author(s): Rahman, Md H., Tsuchiya T., Suwabe, K., Kohori, J., Tomita, R.N., Kagaya, Y., Kobayashii, I., Kakeda, K., Kowyama, Y.
  • Journal Title: Plant Sexual Reproduction (電子版DOI:10.1007/s00497-007-0044-x)
• [Journal Article] Expression of stigma- and anther-specific genes located in the S locus region of Ipomoea trifida. (2007)
  • Author(s): Rahman, Md H., Uchiyama, M., Kuno, M., Hirashima, N., Suwabe, K., Tsuchiya, T., Kagaya, Y., Kobayashii, I., Kakeda, K., Kowyama, Y.
  • Journal Title: Plant Sexual Reproduction (電子版DOI:10.1007/s00497-007-0045-9)
• [Journal Article] DNA methylation and epigenetic inheritance during plant gametogenesis. (2006)
  • Author(s): Takeda, S., Paszkowski, J.
  • Journal Title: Chromosoma 115 Pages: 27-35
• [Journal Article] Chromatin assembly factor 1 ensures the stable maintenance of silent chromatin states in Arabidopsis. (2006)
  • Author(s): Ono, T., Kaya H., Takeda, S., Abe, M., Ogawa, Y., Kato, M., Kakutani, T., Mittelsten Scheid, O., Araki, T., Shibahara, K.
  • Journal Title: Genes Cells 11 Pages: 153-162
• [Presentation] 種子発芽における遺伝子発現プログラムの転換 (2012)
  • Author(s): 服部束穂
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] 種子発芽における遺伝子発現プログラムの転換 (2012)
  • Author(s): 服部束穂、山本章子、村瀬祥子、岩田昇悟、吉居正勝、大崎莉奈、武田真、加賀谷安章
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都(招待講演)
• [Presentation] Seed dormancy and germination : a view from gene expression program switching (2011)
  • Author(s): Hattori, T., Yamamoto, A., Murase, S., Takeda, S., Kagaya, Y.
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: (招待講演)(要旨集)
  • Year and Date: 2011-03-11
• [Presentation] Instability of epigenetic gene regulation in the loss of function mutant of BRU1 in Arabidopsis (2011)
  • Author(s): Ohno, Y., Narangajavana, J., Yamamoto, A., Hattori, T., Kagaya, Y., Paszkowski, J., Gruissem, W., Hennig, L., Takeda
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: (要旨集)
  • Year and Date: 2011-03-11
• [Presentation] Exosome is an endogenous gene silencing sppressor in Arabidopsis (2011)
  • Author(s): Kagaya, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Exosome is an endogenous gene silencing suppressor in Arabidopsis (2011)
  • Author(s): Minami, D., Kagaya, M., Kobayashi, I., Hattori, T., Kagaya, Y.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] イネSNF2ヘリカーゼ様タンパク質をコードするENDOSPERMLESS1 (ENL1)の欠損は胚乳核の分裂異常を示す (2010)
  • Author(s): 原睦美、加藤大和、佐藤豊、北野英己、長戸康郎、石川亮、木下哲、武田真、服部束穂
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] シロイヌナズナの種子休眠性獲得に欠損のある新規変異体の原因遺伝子のクローニング (2010)
  • Author(s): 浅野智也, 皆巳大輔, 加賀谷道子, 加賀谷安章, 服部束穂
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] leafy cotyledon型変異体の異時的性質に関する解析 (2010)
  • Author(s): 山本章子, 加賀谷安章, 村瀬祥子, 松島良, 西村いく子, 黒谷賢, 武田真, 服部束穂
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] Instability of sub-chromosomal gene regulation in the loss-of-function mutant of BRU1, affecting epigenetic inheritance in Arabidopsis. (2010)
  • Author(s): Ohno, Y., Narangajavana, J., Yamamoto, A., Hattori, T., Kagaya, Y., Paszkowski, J., Gruissem, W., Hennig, L, Takeda, S.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction-from pollination to fertilization.
  • Place of Presentation: 奈良
• [Presentation] FUS3の下流でABA生合成制御に関与する遺伝子の変異体分離と表現型解析 (2009)
  • Author(s): 加賀谷安章、皆巳大輔、香村博之、浅野智也、加賀谷道子、佐野順子、服部束穂
  • Organizer: 日本植物生理学会第50回年会
  • Place of Presentation: 名古屋
• [Presentation] ゲノムメンテナンスとエピジェネティック制御に関与するシロイヌナズナ核内因子BRUSHY 1(BRU1)の機能欠損変異体における遺伝子発現プロファイルの解析 (2009)
  • Author(s): 服部束穂、(9名中5番目)武田真(9名中9番目)
  • Organizer: 日本植物生理学会第50回年会
  • Place of Presentation: 名古屋
• [Presentation] FUS3の下流で胚の成長停止を制御するシグナル伝達の解析 (2008)
  • Author(s): 加賀谷安章,堤田久美子,山本章子,鈴木将史,谷口桂太,堺和彦,加賀谷道子,服部束穂
  • Organizer: 日本植物生理学会第48回年会
  • Place of Presentation: 松山
• [Presentation] Modulation of auxin signaling during seed development in Arabidopsis (2007)
  • Author(s): Tsukaho Hattori
  • Organizer: International Plant Growth Substances Association 19th Meeting
  • Place of Presentation: PuretoVallarta, Mexico
• [Presentation] シロイヌナズナ種子成熟過程におけるオーキシンシグナル伝達の変化に関する解析 (2007)
  • Author(s): 加賀谷安章
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Presentation] Modulation of auxin signaling during seed development in Arabidopsis. (2007)
  • Author(s): Kagaya, Y., (10名中1番目), Hattori, T., (10名中10番目)
  • Organizer: International Plant Growth Substances Association 19th Meeting.
  • Place of Presentation: Pureto Vallarta, Mexico
• [Presentation] Modulation of auxin signaling during seed development in Arabidopsis. (2007)
  • Author(s): Yasuaki Kagaya
  • Organizer: International Plant Growth Substances Association 19th Meeting
  • Place of Presentation: Puerto Vallarta, Mexico
• [Book] 種子貯蔵タンパク質遺伝子の制御ネットワーク. 「種子の科学とバイオテクノロジー」 (2009)
  • Author(s): 服部束穂, 原田久也監修, 種子生理化学研究会編
  • Publisher: 学会出版センター
• [Book] 種子の科学とバイオテクノロジー(原田久也監修/種子生理化学研究会編集) (2009)
  • Author(s): 服部束穂(分担執筆)
  • Total Pages: 396
  • Publisher: 学会出版会
• [Book] 種子貯蔵タンパク質遺伝子の制御ネットワーク.「種子のバイオサイエンス(改訂版)」原田久也他編 (2008)
  • Author(s): 服部束穂
  • Publisher: 学会出版センター
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~gaikan/hattori.html