Molecular analysis of the genome barriers in the early reproductive process
Project Area | Genome Barriers in Plant Reproduction |
Project/Area Number |
18075008
|
Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
|
Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
TAKAYAMA Seiji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (70273836)
|
Project Period (FY) |
2006 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥88,500,000 (Direct Cost: ¥88,500,000)
Fiscal Year 2010: ¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 2009: ¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2007: ¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2006: ¥12,700,000 (Direct Cost: ¥12,700,000)
|
Keywords | 生殖 / シグナル伝達 / 植物 / アブラナ科 / 受粉 / ゲノム障壁 / アブラナ科植物 / カルシウム / アクアポリン / 乳頭細胞 / 花粉 / マイクロアレイ / 情報伝達 / アクチン / 液胞 / トモグラフィー / シロイヌナズナ / アクチン結合蛋白質 / 種間障壁 / pollen coat / yellow cameleon |
Research Abstract |
We have analyzed the molecular basis for the pollen-papilla cell interactions which were thought to function as the first genome-barrier during the reproductive process in the Brassicaceae. First we found that the pollen coat contains some factor(s), which induces the rearrangement of actin filaments in the papilla cell of the same species and the secretion of Ca^<2+>-containing water to the pollen. These compatible reactions were found to be inhibited by the self-incompatibility signaling pathway. We also identified the candidate Ca^<2+>-transporter which were expected to function in this compatible pollination process.
|
Report
(7 results)
Research Products
(74 results)
-
[Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility.2010
Author(s)
Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., Takayama, S.
-
Journal Title
Nature 466
Pages: 983-986
Related Report
Peer Reviewed
-
[Journal Article] Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations.2010
Author(s)
Isokawa, S., Osaka, M., Shirasawa, A., Kikuta, R., Komatsu, S., Horisaki, A., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., Watanabe, M.
-
Journal Title
Genes Genetic Systems 85
Pages: 87-96
Related Report
Peer Reviewed
-
[Journal Article] Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene.2010
Author(s)
Tsuchimatsu,T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Staedler, T., Suzuki, G., Takayama, S., Watanabe, M., Shimizu, K.K.
-
Journal Title
Nature 464
Pages: 1342-1346
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Journal Article] Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth.2009
Author(s)
Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., Takayama, S.
-
Journal Title
Plant Physiology 150
Pages: 1-13
Related Report
Peer Reviewed
-
-
[Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination.2007
Author(s)
Iwano, M., Shiba, H., Matoba, K., Miwa, T., Funato, M., Entani, T., Nakayama, P., Shimosato, H., Takaoka, A., Isogai, A., Takayama, S.
-
Journal Title
Plant Physiology 144
Pages: 72-81
Related Report
Peer Reviewed
-
[Journal Article] Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa.2007
Author(s)
Kakita, M., Murase, K., Iwano, M., Matsumoto, T., Watanabe, M., Shiba, H., Isogai, A., Takayama, S.
-
Journal Title
Plant Cell 19
Pages: 3961-3973
Related Report
Peer Reviewed
-
[Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self-nonself recognition in Brassica self-incompatibility.2007
Author(s)
Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F.-S., Watanabe, M., Isogai, A., Takayama, S.
-
Journal Title
Plant Cell 19
Pages: 107-117
Related Report
Peer Reviewed
-
[Journal Article] Two distinct forms of M-Locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce selfincompatibility signaling in Brassica rapa2007
Author(s)
Kakita, M., Murase, K., Iwano, M., Matsumoto, T., Watanabe, M., Shiba, H., Isogai, A., and Takayama, S.
-
Journal Title
Plant Cell 19
Pages: 3961-3973
Related Report
Peer Reviewed
-
[Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination2007
Author(s)
Iwano, M., Shiba, H., Matoba, K., Miwa, T., Funato, M., Entani, T., Nakayama, P., Shimosato, H., Takaoka, A., Isogai, A. and Takayama, S.
-
Journal Title
Plant Physiology 144
Pages: 72-81
Related Report
Peer Reviewed
-
[Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self/nonself recognition in Brassica self-incompatibility2007
Author(s)
Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F.-S., Watanabe, M., Isogai, A., and Takayama, S.
-
Journal Title
Plant Cell 19
Pages: 107-117
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination.2008
Author(s)
Iwano, M., Shiba, H., Matoba, K., Takaoka, A., Isogai, A., Takayama, S.
Organizer
9th APMC
Place of Presentation
Jeju, Korea.
Year and Date
2008-11-04
Related Report
-
[Presentation] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination.2008
Author(s)
Iwano, M., Shiba, H., Matoba, K., Takaoka, A., Isogai, A. and Takayama, S.
Organizer
9th Asia Pacific Microscopy Conference (APMC9)
Place of Presentation
Jeju, Korea
Year and Date
2008-11-04
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-