Molecular analysis of the genome barriers in the early reproductive process

• Project Area: Genome Barriers in Plant Reproduction
• Project/Area Number: 18075008
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAYAMA Seiji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (70273836)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥88,500,000 (Direct Cost: ¥88,500,000); Fiscal Year 2010: ¥22,500,000 (Direct Cost: ¥22,500,000); Fiscal Year 2009: ¥22,500,000 (Direct Cost: ¥22,500,000); Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2007: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2006: ¥12,700,000 (Direct Cost: ¥12,700,000)
• Keywords: 生殖 / シグナル伝達 / 植物 / アブラナ科 / 受粉 / ゲノム障壁 / アブラナ科植物 / カルシウム / アクアポリン / 乳頭細胞 / 花粉 / マイクロアレイ / 情報伝達 / アクチン / 液胞 / トモグラフィー / シロイヌナズナ / アクチン結合蛋白質 / 種間障壁 / pollen coat / yellow cameleon

Research Abstract

We have analyzed the molecular basis for the pollen-papilla cell interactions which were thought to function as the first genome-barrier during the reproductive process in the Brassicaceae. First we found that the pollen coat contains some factor(s), which induces the rearrangement of actin filaments in the papilla cell of the same species and the secretion of Ca^<2+>-containing water to the pollen. These compatible reactions were found to be inhibited by the self-incompatibility signaling pathway. We also identified the candidate Ca^<2+>-transporter which were expected to function in this compatible pollination process.

Research Products

• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility. (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., Takayama, S.
  • Journal Title: Nature 466 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations. (2010)
  • Author(s): Isokawa, S., Osaka, M., Shirasawa, A., Kikuta, R., Komatsu, S., Horisaki, A., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., Watanabe, M.
  • Journal Title: Genes Genetic Systems 85 Pages: 87-96
  • Peer Reviewed
• [Journal Article] Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene. (2010)
  • Author(s): Tsuchimatsu,T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Staedler, T., Suzuki, G., Takayama, S., Watanabe, M., Shimizu, K.K.
  • Journal Title: Nature 464 Pages: 1342-1346
  • Peer Reviewed
• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility (2010)
  • Author(s): Tarutani, Y.
  • Journal Title: Nature Volume: 466 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Novel self-compatible lines of Brassica rapa L.isolated from the Japanese bulk-populations. (2010)
  • Author(s): Isokawa, S.
  • Journal Title: Genes Genet.Syst. Volume: 22 Pages: 87-96
  • Peer Reviewed
• [Journal Article] Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene (2010)
  • Author(s): Tsuchimatsu, T
  • Journal Title: Nature Volume: 464 Pages: 1342-1346
  • Peer Reviewed
• [Journal Article] アブラナの自家不和合性におけるsmall RNAを介した優劣性機構 (2010)
  • Author(s): 樽谷芳明
  • Journal Title: 細胞工学 Volume: 29 Pages: 1254-1256
• [Journal Article] アブラナの自他認識に関わるエピジェネティックな遺伝子発現制御 (2010)
  • Author(s): 柴博史
  • Journal Title: Plant Morphology Volume: 22 Pages: 3-8
• [Journal Article] Novel self-compatible lines of Brassica rapa L.isolated from the Japanese bulk-populations (2010)
  • Author(s): Isokawa, S.
  • Journal Title: Genes Genet. Syst. (In press)
  • Peer Reviewed
• [Journal Article] Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth. (2009)
  • Author(s): Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., Takayama, S.
  • Journal Title: Plant Physiology 150 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Fine-tuning of the cytoplsmic Ca^<2+> concentration is essential for pollen tube erowth (2009)
  • Author(s): Iwano, M.
  • Journal Title: Plant Physiol. 150 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination. (2007)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Miwa, T., Funato, M., Entani, T., Nakayama, P., Shimosato, H., Takaoka, A., Isogai, A., Takayama, S.
  • Journal Title: Plant Physiology 144 Pages: 72-81
  • Peer Reviewed
• [Journal Article] Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa. (2007)
  • Author(s): Kakita, M., Murase, K., Iwano, M., Matsumoto, T., Watanabe, M., Shiba, H., Isogai, A., Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 3961-3973
  • Peer Reviewed
• [Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self-nonself recognition in Brassica self-incompatibility. (2007)
  • Author(s): Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F.-S., Watanabe, M., Isogai, A., Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 107-117
  • Peer Reviewed
• [Journal Article] Two distinct forms of M-Locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce selfincompatibility signaling in Brassica rapa (2007)
  • Author(s): Kakita, M., Murase, K., Iwano, M., Matsumoto, T., Watanabe, M., Shiba, H., Isogai, A., and Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 3961-3973
  • Peer Reviewed
• [Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination (2007)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Miwa, T., Funato, M., Entani, T., Nakayama, P., Shimosato, H., Takaoka, A., Isogai, A. and Takayama, S.
  • Journal Title: Plant Physiology 144 Pages: 72-81
  • Peer Reviewed
• [Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self/nonself recognition in Brassica self-incompatibility (2007)
  • Author(s): Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F.-S., Watanabe, M., Isogai, A., and Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 107-117
  • Peer Reviewed
• [Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross- pollination. (2007)
  • Author(s): M.Iwano
  • Journal Title: Plant Physiology 144.1 Pages: 72-81
  • Peer Reviewed
• [Journal Article] Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa. (2007)
  • Author(s): M.Kakita
  • Journal Title: Plant Cell 19.12 Pages: 3961-3973
  • Peer Reviewed
• [Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination (2007)
  • Author(s): Iwano, M.
  • Journal Title: Plant Physiology (in press)
• [Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self/nonself recognition in Brassica self-incompatibility (2007)
  • Author(s): Shimosato, H.
  • Journal Title: Plant Cell 19・1 Pages: 107-117
• [Journal Article] Direct interaction between S-locus receptor kinase and M-locus protein kinase involved in Brassica self-incompatibility signaling (2007)
  • Author(s): Kakita, M.
  • Journal Title: Plant Biotechnology 24・2 Pages: 185-190
• [Presentation] レーザーマイクロダイセクションを利用したシロイヌナズナ葯タペート組織における遺伝子発現様態の網羅的解析 (2011)
  • Author(s): 木本剛彰
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台市((震災のため中止)要旨集発行をもって発表成立)
  • Year and Date: 2011-03-22
• [Presentation] アブラナ科植物の自家・他家受粉過程における乳頭細胞微細構造の超高圧電顕トモグラフィー解析 (2011)
  • Author(s): 岩野恵
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台市((震災のため中止)要旨集発行をもって発表成立)
  • Year and Date: 2011-03-21
• [Presentation] アブラナ科植物自家不和合性に関わる膜結合型キナーゼMLPKの活性調節機構 (2011)
  • Author(s): 垣田満
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台市((震災のため中止)要旨集発行をもって発表成立)
  • Year and Date: 2011-03-20
• [Presentation] アブラナ科植物の自家不和合反応時における細胞内膜輸送系の関与 (2011)
  • Author(s): 伊藤花菜江
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台市((震災のため)中止要旨集発行をもって発表成立)
  • Year and Date: 2011-03-20
• [Presentation] アブラナ科植物乳頭細胞の三次元電子線トモグラフィーによる解析 (2011)
  • Author(s): 永井里奈
  • Organizer: 近畿電顕技術情報交換会 第30回談話会
  • Place of Presentation: 茨木市
  • Year and Date: 2011-03-19
• [Presentation] Molecular mechanisms of self-incompatibility in the Brassicaceae. (2010)
  • Author(s): Takayama, S
  • Organizer: 21st ICSPR
  • Place of Presentation: Bristol, UK.
  • Year and Date: 2010-08-05
• [Presentation] Molecular mechanisms of self-incompatibility in the Brassicaceae (2010)
  • Author(s): Takayama, S.
  • Organizer: XXI International Congress on Sexual Plant Reproduction (21st ICSPR)
  • Place of Presentation: Bristol, UK
  • Year and Date: 2010-08-05
• [Presentation] Genome-wide analyses of allele-specific expression and DNA methylation in Arabidopsis thaliana (2010)
  • Author(s): Shiba, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-08
• [Presentation] シロイヌナズナ乳頭細胞の3次元電子線トモグラフィーによる解析 (2010)
  • Author(s): 永井里奈
  • Organizer: 医学生物学電子顕微鏡技術学会
  • Place of Presentation: 別府市
  • Year and Date: 2010-05-16
• [Presentation] Self-incompatibility signaling in Brassicaceae. (2010)
  • Author(s): Takayama, S
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction.
  • Place of Presentation: Nara, Japan
  • Year and Date: 2010-05-11
• [Presentation] Self-incompatibility signaling in the Brassicaceae (2010)
  • Author(s): Takayama, S.
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction
  • Place of Presentation: Nara, Japan
• [Presentation] アブラナ科植物の受粉時におけるアクアポリンの役割 (2010)
  • Author(s): 小川宣仁
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] Analysis of molecular mechanism of self-incompatibility in the Brassicaceae (2010)
  • Author(s): Lao, X.
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] アブラナ科植物の雌ずい乳頭細胞で発現する遺伝子群の解析 (2010)
  • Author(s): 大原圭子
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] バイオイメージングによるアブラナ科植物アロ認証機構の解析 (2009)
  • Author(s): 岩野恵
  • Organizer: 第32回日本分子生物学会ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Self-incompatibility signaling in Brassica. (2009)
  • Author(s): Takayama, S
  • Organizer: Symposium in 9th IPMB
  • Place of Presentation: St.Louis, USA.
  • Year and Date: 2009-10-28
• [Presentation] Fine tuning of the cytoplasmic calcium concentration is essential for pollen tube growth. (2009)
  • Author(s): Iwano, M., Isogai, A., Takayama, S.
  • Organizer: 9th IPMB
  • Place of Presentation: St.Louis, USA.
  • Year and Date: 2009-10-28
• [Presentation] アブラナ科植物におけるアロ認識機構 (2009)
  • Author(s): 岩野恵
  • Organizer: 第82回日本生化学会,シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 被子植物が自家受精を回避するメカニズム (2009)
  • Author(s): 高山誠司
  • Organizer: 平成21年度日本動物学会中部支部大会公開シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-08-01
• [Presentation] アブラナ科植物の受粉時おけるアクアポリンの役割 (2009)
  • Author(s): 岩野恵
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 博多
  • Year and Date: 2009-03-29
• [Presentation] アブラナ科植物の自家不和合性に関わる膜結合型キナーゼMLPKの機能解析 (2009)
  • Author(s): 垣田満
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 博多
  • Year and Date: 2009-03-29
• [Presentation] アブラナ科植物でみられる劣性SP11の発現抑制機構に関する研究 (2009)
  • Author(s): 樽谷芳明
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 博多
  • Year and Date: 2009-03-29
• [Presentation] アブラナ科植物の受粉過程において機能するアクアポリンの探索 (2009)
  • Author(s): 岩野恵
  • Organizer: 第50回植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] Self-incompatibility system in Brassica and its application for F1-hybrid breeding. (2009)
  • Author(s): Takayama, S
  • Organizer: KRIBB-NAIST Joint Symposium
  • Place of Presentation: Jeongeup, Korea.
  • Year and Date: 2009-02-26
• [Presentation] Self-incompatibility system in Brassica and its application for F1-hybrid breeding (2009)
  • Author(s): Takayama, S.
  • Organizer: KRIBB-NAIST Joint Symposium
  • Place of Presentation: Jeongeun, Korea
  • Year and Date: 2009-02-26
• [Presentation] Three dimensional analysis of the vacuolar atructure in papilla cells during self- and cross-pollination in Brassicaceae (2009)
  • Author(s): Iwano, M.
  • Organizer: 6th International Symposium on Electron Microscopy in Medicine and Biology 2009
  • Place of Presentation: Kobe, Japan
• [Presentation] Fine tuning of the cytoplasmic calcium concentration is essential for pollen tube growth (2009)
  • Author(s): Iwano, M.
  • Organizer: 9th International Plant Molecular Biology
  • Place of Presentation: St.Louis, USA
• [Presentation] Self-incompatibility signaling in Brassica (2009)
  • Author(s): Takayama, S.
  • Organizer: Symposium "Pollen/Self-incompatibility" in 9th International Plant Molecular Biology (IPMB)
  • Place of Presentation: St.Louis, USA
• [Presentation] Self-incompatibility signaling in Brassica. NAIST GCOE Int. (2008)
  • Author(s): Takayama, S
  • Organizer: Symposium
  • Place of Presentation: Nara, Japan.
  • Year and Date: 2008-11-14
• [Presentation] Self-incompatibility signaling in Brassica (2008)
  • Author(s): Takayama, S.
  • Organizer: NAIST Global COE International Symposium
  • Place of Presentation: Nara
  • Year and Date: 2008-11-14
• [Presentation] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination. (2008)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Takaoka, A., Isogai, A., Takayama, S.
  • Organizer: 9th APMC
  • Place of Presentation: Jeju, Korea.
  • Year and Date: 2008-11-04
• [Presentation] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination. (2008)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Takaoka, A., Isogai, A. and Takayama, S.
  • Organizer: 9th Asia Pacific Microscopy Conference (APMC9)
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-04
• [Presentation] Actin dynamics in Papilla cells of Brassica rapa during self- and cross-pollination (2008)
  • Author(s): Iwano, M.
  • Organizer: 9th Asia Pacific Microscopy Conference
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-01
• [Presentation] アブラナ科植物でみいだされた自家和合性突然変異系統の遺伝学的解析 (2008)
  • Author(s): 五十川祥代
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 横須賀
  • Year and Date: 2008-10-11
• [Presentation] 免疫組織化学法によるアブラナ科植物受粉過程の解析 (2008)
  • Author(s): 岩野恵
  • Organizer: 第49回組織細胞化学会
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-05
• [Presentation] 受粉過程における花粉と乳頭細胞のCa^<2+>ダイナミクス (2008)
  • Author(s): 岩野恵
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知
  • Year and Date: 2008-09-27
• [Presentation] Self-incompatibility signaling in Brassica (2008)
  • Author(s): Takayama, S.
  • Organizer: Japan-Korea Joint Symposium on Plant Growthand Signal Transduction
  • Place of Presentation: Yokohama
  • Year and Date: 2008-06-10
• [Presentation] アブラナ科植物乳頭細胞の三次元電子線トモグラフィードによる解析 (2008)
  • Author(s): 永井里奈
  • Organizer: 医学生物学電子顕微鏡学会第24回学術講演会
  • Place of Presentation: 横須賀
  • Year and Date: 2008-05-18
• [Presentation] アブラナ科植物の受粉・受精過程における花粉管Ca2+のモニタリング (2008)
  • Author(s): 岩野 恵
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-28
• [Presentation] 植物細胞におけるアクチンフィラメント可視化への試み (2007)
  • Author(s): 岩野 恵
  • Organizer: 医学生物学電子顕微鏡技術学会第23回学術講演会(ワークショップ)
  • Place of Presentation: 北九州市
  • Year and Date: 2007-05-20
• [Presentation] アブラナ科植物乳頭細胞液胞の3次元構造の解析 (2007)
  • Author(s): 岩野 恵
  • Organizer: 医学生物学電子顕微鏡技術学会第23回学術講演会
  • Place of Presentation: 北九州市
  • Year and Date: 2007-05-20
• [Book] Self-incompatibility systems in flowering plants. In "Plant Developmental Biology.Biotechnological Perspectives" (Vol. 1)(Edited by Pua, E.C., and Davey, M.R.) (2010)
  • Author(s): Kaothein-Nakayama, P., Isogai, A., Takayama, S.
  • Publisher: Springer, Heidelberg
• [Book] 「植物の自家不和合性機構-自他識別機構の普遍性と多様性」植物のシグナル伝達-分子と応答-(単行本) (2010)
  • Author(s): 垣田満、高山誠司
  • Publisher: 共立出版株式会社
• [Book] "Self-incompatibility system in flowering plant" in Plant Developmental Biology-Biotechnological Perspectives, Vol.1 (2010)
  • Author(s): Kaothien-Nakayama, P.
  • Publisher: Springer
• [Book] Self-incompatibility systems in flowering plants. In "Plant Developmental Biology-Biotechnological Perspectives". (2009)
  • Author(s): Nakayama, P
  • Publisher: Springer-Verlag(in press)
• [Book] Milestones identifying self-incompatibility genes in Brassica species. From old stories to new findings. In “Self-Incompatibility in Flowering Plants : Evolution, Diversity, and Mechanisms"(Edited by Franklin-Tong, V.E.) (2008)
  • Author(s): Watanabe, M., Suzuki, G., Takayama, S.
  • Publisher: Springer, Heidelberg
• [Book] Milestones identifying self-incompatibility genes in Brassica species - From old stories to new findings. In ''Self-Incompatibility in Flowering Plants: Evolution, Diversity, and Mechanisms" (Edited by Franklin-Tong, V.E.) (2008)
  • Author(s): Watanabe, M., Suzuki, G., and Takayama, S.
  • Publisher: Springer, Heidelberg
• [Book] Milestones identifying self-incompatibility genes in Brassica species : from old stories to new findings. In "Self Incompatibility in Flowering Plants" (2008)
  • Author(s): Watanabe, M
  • Total Pages: 22
  • Publisher: Springer-Verlag
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