| Project Area | Proteolysis in the Regulation of Biological Processes |
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| Project/Area Number |
18076001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKUMI Kamura (KAMURA Takumi) Nagoya University, 理学研究科, 教授 (40333455)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGETSUGU Hatakeyama 北海道大学, 大学院・医学研究科, 教授 (70294973)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥140,900,000 (Direct Cost: ¥140,900,000)
Fiscal Year 2010: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2009: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2008: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2007: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2006: ¥20,900,000 (Direct Cost: ¥20,900,000)
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| Keywords | タンパク質分解 / ユビキチン |
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| Research Abstract |
Ubiquitylation and subsequent proteasomal degradation of regulatory proteins control a variety of cellular processes. The ubiquitin conjugation to target proteins is processed by three enzymes, E1, E2, and E3. The E3s are responsible for recognizing and recruiting target proteins for polyubiquitylation. Cullin-based E3 complexes and TRIM E3 families are thought to regulate many cellular events. In this research, we try to clarify the function of Cullin-based E3 complexes and TRIM E3 families. Now we show that Cullin-based E3 complexes are responsible for regulating the function of Mrc1, Ctf4, Elongin A, p53, and Ypt53. We also identify that Lag2 negatively modulate the activity of SCF complex. Finally, we demonstrate that TRIM E3 families control the function of estrogen receptor, androgen receptor, Abi2, p53, PIAS3 and Ikkγ. These observations thus idicate that Cullin-based E3 complexes and TRIM E3 families plays an important role in a variety of cellular processes by regulating the function of many target proteins.
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