| Budget Amount *help |
¥136,000,000 (Direct Cost: ¥136,000,000)
Fiscal Year 2010: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2009: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2008: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2007: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2006: ¥22,000,000 (Direct Cost: ¥22,000,000)
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| Research Abstract |
Structural studies of proteins and protein complexes related to the ubiquitin-proteasome system and autophagy were conducted using X-ray crystallography, NMR and small-angle neutron scattering techniques. The details of the ubiquitin interaction mode of ubiquitin receptors,the working mechanism of E2 (UbcH5b) and the activation mechanism of SCF E3 by NEDD8 were revealed. We also determined the structures of deconjugating enzyme Atg4B bound to LC3, E1-like enzyme Atg7, Atg12-Atg5 conjugate, and Atg8-Atg19 and LC3-p62 complexes, clarifying the molecular mechanisms of reversible Atg8 lipidation and autophagic receptor recognition byAtg8/LC3.
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