| Project Area | Molecular interaction and modal shift of cellular sensors |
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| Project/Area Number |
18077003
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
WATANABE Masakatsu The Graduate University for Advanced Studies, 先導科学研究科, 教授 (40124226)
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| Co-Investigator(Kenkyū-buntansha) |
ISEKI Mineo 総合研究大学院大学, 学融合推進センター, 特別研究員 (60414009)
OGAWA Kazuo 自然科学研究機構, 岡崎共通研究アイソトープ実験センター, 准教授 (30132731)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥63,500,000 (Direct Cost: ¥63,500,000)
Fiscal Year 2010: ¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2009: ¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2008: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2007: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2006: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | 光センサー / フラビン / 環状AMP / ドメイン相互作用 / 細胞行動 / 光生物学 / 生体機能利用 / タンパク質 |
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| Research Abstract |
Biophysical studies performed to elucidate the mechanisms how the euglenoid pbotoreceptor photoactivated adenylyl cyclase (PAC) converts light signal to cyclic AMP, the versatile second messenger, have led to the following outcomes : electron micrographic details of its tetrameric structure, spectroscopic unveiling of its two states of hydrogen bond networks, division of labor between high-sensitivity-low-speed and the opposite subunits, invention of the single-protein infrared spectroscopic method, photomanipulation of neuronal and behavioral activities of various organisms by heterologous expression of PAC, and elucidation of PAC gene localization along the Euglena chromosomes.
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