| Project Area | Molecular interaction and modal shift of cellular sensors |
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| Project/Area Number |
18077006
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
UETA Yoichi University of Occupational and Environmental Health, Japan, 医学部, 教授 (10232745)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATOMO Toshihisa 産業医科大学, 大学病院, 准教授 (50258604)
FUJIHARA Hiroaki 徳島大学, 大学院・ヘルスバイオサイエンス部, 助教 (10369051)
YOKOYAMA Toru 自治医科大学, 医学部, 助教 (80425321)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥63,800,000 (Direct Cost: ¥63,800,000)
Fiscal Year 2010: ¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2009: ¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2008: ¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2007: ¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2006: ¥12,900,000 (Direct Cost: ¥12,900,000)
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| Keywords | セルセンサー / 浸透圧 / バゾプレッシン / 生体リズム / 緑色蛍光タンパク / トランスジェニック動物 / 視交叉上核 / 時計遺伝子 / モーダルシフト / 生体分子 / 神経ペプチド / シチプス / TRP / GFP / ホルモン / グレリン / パッチクランプ法 / シナプス入力 / 視床下部 / アンジオテンシンII / サリューシン |
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| Research Abstract |
The purpose of the study was to examine the relationship between sensing the hormones (hormone-sensor) secreted by changes of osmolality and sensing the osmolality (osmo-sensor). In particular, we examined arginine vasopressin (AVP) system and drinking behavior. We obtained significant results as follow. (1) In in vitro brain slice preparation including the supraoptic nucleus (SON) that contain AVP neurons whole-cell patch-clamp recordings revealed effects of physiologically active substances on the excitatory and inhibitory synaptic currents in the SON. (2) Whole-cell patch-clamp recordings from an isolated single AVP-eGFP neuron revealed the electrophysiological properties. The diurnal changes of the expression of the several genes and electrophysiological properties were determined in the AVP-eGFP neurons in the suprachiasmatic nucleus that is known to be a circadian center. (3) Central effects of angiotensin II etc on drinking behavior were examined in rats.
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