| Budget Amount *help |
¥91,300,000 (Direct Cost: ¥91,300,000)
Fiscal Year 2010: ¥17,500,000 (Direct Cost: ¥17,500,000)
Fiscal Year 2009: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 2008: ¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 2007: ¥17,100,000 (Direct Cost: ¥17,100,000)
Fiscal Year 2006: ¥20,900,000 (Direct Cost: ¥20,900,000)
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| Research Abstract |
(1) We found that TRPV4 in hippocampal pyramidal neurons is activated at body temperature and involved in the formation of resting membrane potential which could regulate neuronal excitability. (2) We found that TRPV4 in bladder epithelial cells detects wall extension under body temperature and that the Ca^<2+> influx through TRPV4 activation causes ATP release through which the stretch information is transmitted to sensory neurons. (3) We found that mainly TRPV3 detect ambient temperature in skin keratinocytes and that the temperature information is transmitted to sensory neurons through ATP. (4) Single particle analysis with cryoEM revealed 3-demensional structure of TRPV4 at 3.5 nm level. (5) Activation of TRPV4 in skin keratinocytes causes Ca^<2+> influx, strengthening the junctional structure of adherence and tight junctions, which could cause enhancement of the skin barrier functions. (6) We cloned western clawed-frog TRPV3 gene and found that western clawed-frog TRPV3 is activated by cold temperature bellow 17 degree C, which is quite different from the warm temperature activation of mammalian TRPV3.
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