Mechanisms and physiological roles of voltage-sensing phosphatase

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 18077013
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University (2008-2010); National Institutes of Natural Sciences Okazaki Research Facilities (2006-2007)
• Principal Investigator: OKAMURA Yasushi 大阪大学, 医学系研究科, 教授 (80201987)
• Co-Investigator(Kenkyū-buntansha): HIGASHIJIMA Shinichi 自然科学研究機構, 生理学研究所, 准教授 (80270479) ; OKOCHI Yoshifumi 大阪大学, 医学系研究科, 助教 (90435818) ; IWASAKI Hirohide 自然科学研究機構, 生理学研究所, 助教 (30342752)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥72,800,000 (Direct Cost: ¥72,800,000); Fiscal Year 2010: ¥14,200,000 (Direct Cost: ¥14,200,000); Fiscal Year 2009: ¥14,200,000 (Direct Cost: ¥14,200,000); Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2007: ¥14,500,000 (Direct Cost: ¥14,500,000); Fiscal Year 2006: ¥14,500,000 (Direct Cost: ¥14,500,000)
• Keywords: イオンチャネル / 酵素 / シグナル伝達 / 脂質 / ホスファターゼ / 生体膜 / 膜電位 / 生体分子 / 生理学 / 蛋白質 / イノシトールリン脂質 / 脱分極 / GFP / 細胞膜

Research Abstract

Voltage sensing phosphatase gene is conserved in genomes from sea squirt to human. VSP consists of the voltage sensing domain and the cytoplasmic enzyme region that operates as the phosphoinositide phosphatase. Mechanisms of VSP have been examined by integrative approaches including electrophysiology, in vitro phosphatase assay, mathematical modeling analysis and imaging analysis. Results showed that voltage sensor activates PI(4,5)P2 and PIP3 phosphatase activity upon depolarization and this coupling occurs over the entire range of voltage sensitivity of the voltage sensor. We also found that the state of enzyme affects the motions of the voltage sensor suggesting the presence of retrograde interaction from enzyme to the voltage sensor.

Research Products

• [Journal Article] Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity (2011)
  • Author(s): Sakata S, Hossain MI, Okamura Y
  • Journal Title: The Journal of Physiology Volume: 589 Pages: 2687-705
• [Journal Article] Voltage-sensing phosphatase : its molecular relationship with PTEN (2011)
  • Author(s): Okamura Y & Dixon JE
  • Journal Title: Physiology Volume: 26(1) Pages: 6-13
• [Journal Article] A mechanism for graded motor control encoded in the channel properties of the muscle ACh receptor (2011)
  • Author(s): Nishino A, Baba SA, Okamura Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Pages: 2599-604
  • Peer Reviewed
• [Journal Article] 電位依存性プロトンチャネルに見る膜電位センサーの謎:細胞膜中に存在する電荷 (2011)
  • Author(s): 黒川竜紀、岡村康司
  • Journal Title: 生物物理 Volume: (In press)
  • Peer Reviewed
• [Journal Article] Voltage-Sensing Phosphatase : Its Molecular Relationship With PTEN (2011)
  • Author(s): Yasushi Okamura, Jack E.Dixon
  • Journal Title: Physiology Volume: 26(In press)
  • Peer Reviewed
• [Journal Article] Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian (2010)
  • Author(s): Ogasawara M, Sasaki M, Nakazawa N, Nishino A & Okamura Y
  • Journal Title: Gene Expression Patterns Volume: 11 Pages: 233-8
• [Journal Article] A glycine receptor is involved in the organization of swimming movements in an invertebrate chordate (2010)
  • Author(s): Nishino A, Okamura Y, Piscopo S, Brown ER
  • Journal Title: BMC Neurosci Volume: 6
  • Peer Reviewed
• [Journal Article] Lipids : PI couples voltage to catalysis (2010)
  • Author(s): Okamura Y
  • Journal Title: Nat Chem Biol Volume: 6 Pages: 315-6
• [Journal Article] Visualizing voltage dynamics in zebrafish heart (2010)
  • Author(s): Tsutsui H, Higashijima S, Miyawaki A, Okamura Y
  • Journal Title: J Physiol Volume: 588 Pages: 2017-21
  • NAID: 10030455833
  • Peer Reviewed
• [Journal Article] Single or double? Think zinc! (2010)
  • Author(s): Okamura Y
  • Journal Title: J Physiol Volume: 588 Pages: 1803-4
• [Journal Article] A global synergy of ions and voltage? (2010)
  • Author(s): Okamura Y.
  • Journal Title: J Physiol Volume: 588 Pages: 4649-4649
• [Journal Article] Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian (2010)
  • Author(s): Ogasawara M, Sasaki M, Nakazawa N, Nishino A, Okamura Y
  • Journal Title: Gene Expr Patterns Volume: Dec 21(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Functionality of the voltage-gated proton channel truncated in S4. (2010)
  • Author(s): Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G, Okamura Y.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 107 Pages: 845-855
  • Peer Reviewed
• [Journal Article] VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification (2010)
  • Author(s): El Chemaly A, Okochi Y, Sasaki M, Arnaudeau S, Okamura Y, Demaurex N.
  • Journal Title: Journal of Experimental Medicine 207 Pages: 129-139
  • Peer Reviewed
• [Journal Article] A glycine receptor is involved in the organization of swimming movements in an invertebrate chordate. (2010)
  • Author(s): Nishino A, Okamura Y, Piscopo S, Brown ER.
  • Journal Title: BMC Neurosci. 11 Pages: 6-6
  • Peer Reviewed
• [Journal Article] Voltage-sensing phosphatase : actions and potentials (2009)
  • Author(s): Okamura Y, Murata Y & Iwasaki H
  • Journal Title: J. Physiol. Topical Review Volume: 587(3) Pages: 513-520
• [Journal Article] Voltage-sensing phosphatase : actions and potentials (2009)
  • Author(s): Okamura Y, Murata Y, Iwasaki H.
  • Journal Title: Journal of Physiology 587 Pages: 513-520
  • Peer Reviewed
• [Journal Article] Another story of arginines in voltage sensing : the role of phosphoinositides in coupling voltage sensing to enzyme activity (2009)
  • Author(s): Okamura Y.
  • Journal Title: Journal of General Physiology 134 Pages: 1-4
• [Journal Article] Voltage-gated proton channel is expressed on phagosomes (2009)
  • Author(s): Okochi Y, Sasaki M, Iwasaki H, Okamura Y.
  • Journal Title: Biochem Biophys Res Commun. 382(2) : 274-9. 382 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Voltage-sensing phosphatase : actions and potentials (2009)
  • Author(s): Okamura Y, Murata Y & Iwasaki H
  • Journal Title: J. Physiol. 587(3) Pages: 513-520
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain, M.I., Iwasaki, H., Okochi, Y., Chahine, M., Higashijima, S., Nagayama K. & Okamura, Y.
  • Journal Title: J. Biol. Chem. Volume: 283 Pages: 18248-18259
• [Journal Article] A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate (2008)
  • Author(s): Iwasaki H, Murata Y, Kim Y, Hossain MI, Worby CA, Dixon JE, McCormack T, Sasaki T & Okamura, Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 105 Pages: 7970-7975
• [Journal Article] Improving membrane voltage measurements using FRET with new fluorescent proteins (2008)
  • Author(s): Tsutsui, H., Karasawa, S., Okamura, Y & Miyawaki, A.
  • Journal Title: Nature Methods Volume: 8 Pages: 683-685
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain MI , Iwasaki H, Okochi Y, Chahine, M, Higashijima S, Nagayama K & Okamura Y
  • Journal Title: J. Biol. Chem. 283 Pages: 18248-18259
• [Journal Article] Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch HP, Kurokawa T, Okochi Y, Sasaki M, Okamura Y & Larsson HP
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105(26) Pages: 9111-6
• [Journal Article] A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate (2008)
  • Author(s): Iwasaki H, Murata Y, Kim Y, Hossain MI, Worby CA, Dixon JE, McCormack T, Sasaki T & Okamura Y
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 7970-7975
• [Journal Article] Improving membrane voltage measurements using FRET with new fluorescent proteins (2008)
  • Author(s): Tsutsui H, Karasawa S, Okamura Y & Miyawaki A
  • Journal Title: Nature Methods 8 Pages: 683-5
• [Journal Article] Detailed comparison of expressed and native voltage-gated proton channel currents (2008)
  • Author(s): Musset, B., Cherny, V. V., Morgan, D., Okamura Y, Ramsey, I. S., Clapham, D. E., & DeCoursey T. E.
  • Journal Title: J. Physiol 586 Pages: 2477-2486
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain MI, Iwasaki H, Okochi Y, Chahine M, Higashijiina S, Nagayama K., & Okamura Y.
  • Journal Title: J. Biol. Chem 283 Pages: 18248-18259
  • Peer Reviewed
• [Journal Article] Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch, HP, Kurokawa T, Okochi Y, Sasaki M, Okamura Y, & Larsson HP
  • Journal Title: Proc. Natl. Acad. ScL U.S.A. 105 Pages: 9111-9116
  • Peer Reviewed
• [Journal Article] A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4, 5-bisphosphate (2008)
  • Author(s): Iwasaki H, Murata Y, Kim Y, Hossain MI, Worby CA, Dixon JE, McCormack T, Sasaki T, & Okamura Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 7970-7975
  • Peer Reviewed
• [Journal Article] Improving membrane voltage measurements using FRET with new fluorescent proteins (2008)
  • Author(s): Tsutsui H, Karasawa S, Okamura Y, & Miyawaki A
  • Journal Title: Nature Methods 8 Pages: 683-685
  • Peer Reviewed
• [Journal Article] Ion channel clustering at the axon initial segment and node of Ranvier evolved sequentially in early chordates (2008)
  • Author(s): Hill AS, Nishino A, Nakajo K, Zhang G, Fineman JR, Selzer ME, Okamura Y, & Cooper EC
  • Journal Title: PLoS Genet 4(12)
  • Peer Reviewed
• [Journal Article] Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2 (2007)
  • Author(s): Murata Y & Okamura Y.
  • Journal Title: J. Physiol. Volume: 583 Pages: 875-889
• [Journal Article] Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2 (2007)
  • Author(s): Murata Y & Okamura Y
  • Journal Title: J. Physiol. 583 Pages: 875-889
• [Journal Article] Biodiversity of voltage sensor domain proteins (2007)
  • Author(s): Okamura Y
  • Journal Title: (review) Pflugers Archiv. 454(3) Pages: 361-71
• [Journal Article] Voltage-dependent calcium influx mediates maturation of myofibril arrangement inascidian larval muscle (2007)
  • Author(s): Ohtsuka, Y.&Okamura, Y.
  • Journal Title: Dev.Biol 301 Pages: 361-373
  • Peer Reviewed
• [Journal Article] Depolarization activates the phosphoinositide phosphatase Ci-VSP,as detected in Xenopus oocytes coexpressing sensors of PIP2 (2007)
  • Author(s): Murata, Y.&Okamura, Y.
  • Journal Title: J.Physiol 583 Pages: 875-889
  • Peer Reviewed
• [Journal Article] Biodiversity of voltage snsor domain proteins (2007)
  • Author(s): Okamura, Y.
  • Journal Title: Pflugers Archiv 454 Pages: 361-371
  • Peer Reviewed
• [Journal Article] ファゴサイトーシスと膜電位変化 (2007)
  • Author(s): 圏村 康司、佐々木 真理
  • Journal Title: 生化学 79(5) Pages: 454-458
  • Peer Reviewed
• [Journal Article] Voltage-dependent calcium influx mediates maturation of myofibril arrangement in ascidian larval muscle (2007)
  • Author(s): Ohtsuka, Y., Okamura, Y
  • Journal Title: Developmental Biology 301 Pages: 1-73
• [Journal Article] A voltage sensor-domain protein is a voltage-gated proton channel (2006)
  • Author(s): Sasaki M, Takagi M & Okamura Y.
  • Journal Title: Science 312(5773) Pages: 589-92
• [Journal Article] A voltage sensor-domain protein is a voltage-gated proton channel (2006)
  • Author(s): Sasaki, M., Takagi, M., Okamura, Y
  • Journal Title: Science 312 Pages: 589-592
• [Journal Article] Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Navl.6 (2006)
  • Author(s): Shirahata, E., Iwasaki, H., Takagi, M., Changqing Lin, Vann Bennett, Okamura, Y., Hayasaka, K.
  • Journal Title: J Neurophysiol 96 Pages: 1347-1357
• [Journal Article] Detailed comparison of expressed and native voltage-gated proton channel currents
  • Author(s): Musset, B., chemy, V.V., Morgan, D., OkamuraY, Ramsey, I.S., Clapham, D.E.&DeCoursey, T.E.
  • Journal Title: J.Physiol. 印刷中
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs
  • Author(s): Hossain, M.I., Iwasaki, H., Okochi, Y., Chahine, M., Higashijima, S., NagayamaK&Okamura.Y.
  • Journal Title: J.Biol.Chem. 印刷中
  • Peer Reviewed
• [Journal Article] Multimeric nature of voltage-gated proton channels
  • Author(s): Kochi, H., Kurokawa, T., Okochi, Y., Sasaki, M., Okamura, Y., &Larsson, H.P.
  • Journal Title: Proc.Natil.Acad.Sci. 印刷中
  • Peer Reviewed
• [Journal Article] Functional analysis of synaptotagmin gene regulatory regions in two distantly related ascidian species
  • Author(s): Matsumoto, J., Katsuyama, Y., Ohtsuka, Y., Lemaire, P., &Okamura, Y.
  • Journal Title: Develop, Growth&Differ. 印刷中
  • Peer Reviewed
• [Presentation] 電位依存性ホスファターゼVSPの基質特異性:PTENとの違い (2011)
  • Author(s): 岡村康司、黒川竜紀、松田真、坂田宗平、本間光一、中川敦史
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)(招待講演)
  • Year and Date: 2011-03-29
• [Presentation] 構造に基づいた電位感受性ホスファターゼの其質特異性の解析 (2011)
  • Author(s): 黒川竜紀、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] PI(4,5)P_2の可視化と数理モデルの適用による電位依存性ホスファターゼCi-VSPの酵素活性の計測 (2011)
  • Author(s): 坂田宗平、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] 貧食細胞における電位依存性プロトンチャネルVSOPによる活性酸素産生制御の解析 (2011)
  • Author(s): 大河内善史、岡村康司、河原司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] 電位依存性プロトンチャネルの温度感受性ゲーティングは2量体会合構造安定性により決定する (2011)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: 神奈川(横浜)
  • Year and Date: 2011-03-28
• [Presentation] Voltage-sensing phosphatase : mechanisms and applications (2011)
  • Author(s): Yasushi Okamura
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 神奈川(横浜)(招待講演)
  • Year and Date: 2011-03-24
• [Presentation] Stability of the Cytoplasmic Dimer Assembly Regulates the Thermosensitive Gating of the Voltage-gated H+ Channel (2011)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、小林恵、中川敦史、岡村康司
  • Organizer: 第55回生物物理学会大会(米国)
  • Place of Presentation: ボルチモア(米国)
  • Year and Date: 2011-03-08
• [Presentation] 電位依存性ホスファターゼVSPの動作原理 特定領域研究 (2010)
  • Author(s): 岡村康司
  • Organizer: 公開シンポジウム細胞感覚 : セルセンサーのモーダルシフト
  • Place of Presentation: 東京
  • Year and Date: 2010-12-23
• [Presentation] 電位依存性ホスファターゼVSPの動作原理 (2010)
  • Author(s): 岡村康司
  • Organizer: 特定領域研究公開シンポジウム細胞感覚:セルセンサーのモーダルシフト
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-12-23
• [Presentation] 膜電位依存性ホスファターゼCi-VSPの電位依存的な酵素活性の計測とモデリング (2010)
  • Author(s): 坂田宗平、岡村康司
  • Organizer: 第103回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-02
• [Presentation] 電位依存性プロトンチャネル細胞内領域の結晶構造と機能的意義 (2010)
  • Author(s): 藤原祐一郎、岡村康司
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 愛知(岡崎)(招待講演)
  • Year and Date: 2010-09-16
• [Presentation] 電位依存性プロトンチャネルにおけるS4トポロジー解析 (2010)
  • Author(s): 黒川竜紀、岡村康司
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 愛知(岡崎)(招待講演)
  • Year and Date: 2010-09-16
• [Presentation] 電位依存性プロトンチャネルVSOPのトポロジー解析 (2010)
  • Author(s): 黒川竜紀、岡村康司
  • Organizer: Neuro2010(第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] 膜電位感受性ホスファターゼVSPの細胞質領域のX線結晶構造解析 (2010)
  • Author(s): 松田真、坂田宗平、竹下浩平、鈴木守、山下栄樹、岡村康司、中川敦史
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-18
• [Presentation] 電位感受性ホスファターゼの動作原理と役割 (2010)
  • Author(s): 岡村康司、黒川竜紀、坂田宗平、平郁子、大河内善史、山口真二、小笠原道生、本間光一
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡(シンポジウム)
  • Year and Date: 2010-05-21
• [Presentation] 電位感受性ホスファターゼの動作原理と役割 (2010)
  • Author(s): 岡村康司、黒川竜紀、坂田宗平、平郁子、大河内善史、山口真二、小笠原道生、本間光一
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡(招待講演)
  • Year and Date: 2010-05-21
• [Presentation] 電位依存性プロトンチャネル細胞内コイルドコイル領域のX線結晶構造解析と機能解析 (2010)
  • Author(s): 藤原祐一郎、黒川竜紀、竹下浩平、中川敦史、岡村康司
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-21
• [Presentation] 電位依存性プロトンチャネルにおけるS4のトポロジー解析 (2010)
  • Author(s): 黒川竜紀、岡村康司
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡(招待講演)
  • Year and Date: 2010-05-20
• [Presentation] ホスホイノシチドの急速な脱リン酸化による一過性TRPC6チャネルの不活性化 (2010)
  • Author(s): 今井裕子、森誠之、岡村康司、井上隆司
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡(招待講演)
  • Year and Date: 2010-05-20
• [Presentation] 電位センサーの動きが飽和した高い膜電位における電位依存性ホスファターゼの酵素活性の解析 (2010)
  • Author(s): 坂田宗平、岡村康司
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] VSOP/Hv1 proton channels sustain superoxide production, calcium entry, and cell migration by limiting the depolarization and acidification of activated neutrophils. (2010)
  • Author(s): Chemaly AE, Okochi Y, Sasaki M, Arnandeau S, Okamura Y, Demaurex N.
  • Organizer: Biophysical society meeting
  • Place of Presentation: San Fransicso、U.S.A.
  • Year and Date: 2010-02-24
• [Presentation] Membrane topology of S4 0f the mouse voltage-gated proton channel (2010)
  • Author(s): Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G, Okamura Y.
  • Organizer: Biophysical society meeting
  • Place of Presentation: San Fransicso、U.S.A.
  • Year and Date: 2010-02-23
• [Presentation] 電位センサータンパクから見た、膜電位-化学関連の機構と役割 (2010)
  • Author(s): 岡村康司
  • Organizer: 岡崎統合バイオサイエンスセンター10周年シンポジウム
  • Place of Presentation: 愛知(岡崎)(招待講演)
  • Year and Date: 2010-02-12
• [Presentation] Electrical manipulation of phosphatidylinositol lipids signaling by voltage sensitive phosphatase in living cells (2009)
  • Author(s): Sato M, Okamura Y, Ueda M
  • Organizer: 第47回日本生物物理学会
  • Place of Presentation: アスティ徳島(徳島県)
  • Year and Date: 2009-10-31
• [Presentation] Avian voltage-sensor containing phosphatase provokes the morphological changes in fibroblasts by its phosphatase activity (2009)
  • Author(s): 平郁子、山口真二、山田マミ、神垣ひろこ、黒川竜紀、岡村康司、本間光一
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Avian voltage-sensor containing phosphatase provokes the morphological changes in fibroblasts by its phosphatase activity (2009)
  • Author(s): Fujii-Taira I, Yamaguchi S, Yamada M, Kamigaki H, Kurokawa T, Okamura Y, Homma KJ
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2009-10-24
• [Presentation] ホヤゲノムから見出された電位センサー蛋白の動作原理と応用 (2009)
  • Author(s): 岡村康司
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2009-10-22
• [Presentation] Visualization of electrical activities with a new voltage probe derived from voltage-sensing phosphatase (2009)
  • Author(s): Tsutsui H, Karasawa S, Miyawaki A, Okamura Y
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋(シンポジウム)
  • Year and Date: 2009-09-17
• [Presentation] Voltage-gated proton channel is expressed on phagosome (2009)
  • Author(s): Okochi Y, Sasaki M, Okamura Y
  • Organizer: 第32回日本神経科学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16
• [Presentation] Mechanisms and physiological roles of voltage-sensor domain proteins (2009)
  • Author(s): Okamura Y
  • Organizer: BBSRC Jana Partnering Program 2007-2011
  • Place of Presentation: ベネッセハウス直島(岡山県)
  • Year and Date: 2009-08-03
• [Presentation] Mechanisms and roles of voltage-sensing proteins without pore structure (2009)
  • Author(s): Okamura Y
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-08-01
• [Presentation] Membrane potential measurements with engineered FRET sensor (2009)
  • Author(s): Tsutsui H, Karasawa S, Miyawaki A, Okamura Y
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-08-01
• [Presentation] The role of C-terminal cytoplasmic regions in dimerization of voltage-gated proton channel (2009)
  • Author(s): Kurokawa K, Okochi Y, Okamura Y
  • Organizer: 36th International congress of physiological sciences
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-30
• [Presentation] Voltage-dependent phosphatase activities of Ci-VSP at high membrane potential. (2009)
  • Author(s): Sakata S, Hossain M.S., Okamura Y.
  • Organizer: 窮36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-30
• [Presentation] Alteration of cellular phosphoinositides by voltage-sensing phosphatases (2009)
  • Author(s): Okamura Y, Hossain M.I.
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-30
• [Presentation] Hv1 proton channels sustain superoxide production and calcium signaling in neutrophils (2009)
  • Author(s): Chemaly A.El, Okochi Y, Sasaki M, Arnaudeau, Okamura Y, Demaurex N.
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-30
• [Presentation] Analysis of voltage-gated proton channel, VSOP, in phagocytes (2009)
  • Author(s): Okochi Y, Sasaki M, Okamura Y
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-30
• [Presentation] KCNE1 modulates KCNQ1 through specific amino acid residues in the pore region (2009)
  • Author(s): Nakajo K, Nishino A, Okamura Y, Kubo Y
  • Organizer: 第36回国際生理学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2009-07-29
• [Presentation] Mechanisms of physiological roles of voltage sensing proteins without pore structure, Whole Day Symposium, Cell sensors (2009)
  • Author(s): Okamura Y
  • Organizer: 36th International Physiological Society Meeting (IUPS)
  • Place of Presentation: Kyoto(talk)
  • Year and Date: 2009-07-27
• [Presentation] Mechanisms of the voltage-sensor domain proteins (2009)
  • Author(s): Okamura Y
  • Organizer: Harbin Conference on Ion Channels in Technology and Drug Discovery
  • Place of Presentation: Harbin, China
  • Year and Date: 2009-07-15
• [Presentation] Mechanisms of the voltage-sensor domain proteins (2009)
  • Author(s): Okamura Y, Kurokawa T, Sakata S, Takagi M
  • Organizer: 2009 Harbin Conference on Ion Channels in Technology and Drug Discovery
  • Place of Presentation: Harbin, China
  • Year and Date: 2009-07-15
• [Presentation] Nicotinic acetylcholine receptors in Ciona larval muscle and their physiological roles in shaping the swimming form (2009)
  • Author(s): Nishino A, Baba S, Okamura Y
  • Organizer: 5^<th> International Tunicate Meeting
  • Place of Presentation: 沖縄産業振興会館(沖縄県)
  • Year and Date: 2009-06-25
• [Presentation] Novel roles of voltage sensor domain discovered from ascidian genomec : voltage-sensing phosphatase (VSP) and voltage-gated proton channel (VSOP/Hv1) (2009)
  • Author(s): Sakata S, Okamura Y
  • Organizer: 5^<th> International Tunicate Meeting
  • Place of Presentation: 沖縄産業振興会館(沖縄県)
  • Year and Date: 2009-06-23
• [Presentation] Novel roles of voltage sensor domain discovered from ascidian genome : voltage-sensing phosphatase (VSP) and voltage-gated proton channel (VSOP1/Hv1) (2009)
  • Author(s): Sakata S, Okamura Y
  • Organizer: 5th Internatinal Tunicate Meeting
  • Place of Presentation: Okinawa
  • Year and Date: 2009-06-21
• [Presentation] A Voltage Sensitive Phosphatase from Xenopus Laevis Testis (2009)
  • Author(s): Ratzan WJ, Okamura Y, Jaffe LA.
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-04
• [Presentation] Improved FRET Sensing Of Membrane Voltage With Voltage Sensitive Phosphatase And New Coral Fluorescence Proteins (2009)
  • Author(s): Tsutsui H, Karasawa S, Miyawaki A, Okamura Y.
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-03
• [Presentation] VSOP Protein Lacking the C-terminal Half of S4-like Segment Retains Proton Permeation (2009)
  • Author(s): 黒川竜紀、高木正浩、坂田宗平、大河内善史、岡村康司
  • Organizer: Biophysical society meeting、ポスター
  • Year and Date: 2009-03-03
• [Presentation] VSOP Protein Lacking the C-terminal Half of S4-like Segment Retains Proton Permeation (2009)
  • Author(s): 黒川竜紀, 高木正浩, 坂田宗平, 大河内善史, 岡村康司
  • Organizer: Biophysical Society Meeting
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2009-03-03
• [Presentation] Does S4 contribute to proton permeationof voltage-gated proton channel? (2009)
  • Author(s): 岡村康司
  • Organizer: ゴードンリサーチコンファレンス"Protons and Membrane Reactions"
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2009-02-24
• [Presentation] 電位依存性プロトンチャネルの動作機構 (2008)
  • Author(s): 黒川 竜紀、コッホ ハンス、高木 正浩、大河内 善史、佐々木 真理、ラーソン ピーター 、 岡村康司
  • Organizer: 第32回日本分子生物学会年会 第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 電位依存性プロトンチャネルの動作機構 (2008)
  • Author(s): 黒川竜紀, コッホハンス, 高木正浩, 大河内善史, 佐々木真理, ラーソンピーター, 岡村康司
  • Organizer: 第32回日本分子生物学会年会第82回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 高膜電位におけるCi-VSPの電位依存的なホスファターゼ活性の解析 (2008)
  • Author(s): 坂田宗平, Hossain MI, 岡村康司
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: 東京(ポスター)
  • Year and Date: 2008-09-13
• [Presentation] 高膜電位におけるCi-VSPの電位依存的なホスファターゼ活性の解析 (2008)
  • Author(s): 坂田宗平, Hossain MI, 岡村康司
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-13
• [Presentation] 電位依存性プロトンチャネルのダイマー形成 (2008)
  • Author(s): 黒川竜紀, Hans P. Koch, 大河内善史, 佐々木真理, H. Peter Larsson, 岡村康司
  • Organizer: 第101回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-13
• [Presentation] 膜電位依存的ボスファターゼCi-VSPの高膜電位における活性 (2008)
  • Author(s): 坂田宗平, ホサイン・イスライル, 岡村康司
  • Organizer: 第101回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-13
• [Presentation] ホヤから見いだされた新規電位センサー蛋白 (2008)
  • Author(s): 岡村康司
  • Organizer: 日本動物学会第79回大会シンポジウム「海産動物を用いた基礎生物学エポック研究」
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] ホヤから見いたされた新規電位センサー蛋白 (2008)
  • Author(s): 岡村康司
  • Organizer: 日本動物学会第79回大会、シンポジウム「海産動物を用いた基礎生物学エポック研究」
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] 電位センサー蛋白の多様性から見た細胞膜電位シグナル伝達機構の進化 (2008)
  • Author(s): 岡村康司、Thomas McCormack、黒川竜紀、斎藤成也
  • Organizer: 第10回日本進化学会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-08-24
• [Presentation] 電位センサー蛋白の多様性から見た細胞膜電位シグナル伝達機構の進化 (2008)
  • Author(s): 岡村康司, Thomas McComack, 黒川竜紀, 斎藤成也
  • Organizer: 第10回日本進化学会シンポジウム「細胞感覚」
  • Place of Presentation: 東京
  • Year and Date: 2008-08-24
• [Presentation] 電位依存性プロトンチャネルVSOPのダイマー形成 (2008)
  • Author(s): 黒川竜紀, Hans Koch, 大河内善史, 佐々木真理, Peter Larsson, 岡村康司
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Biochemical characterization of voltage-gated proton channel, VSOP, in phagocytes (2008)
  • Author(s): Okochi Y, Sasaki M, Iwasaki H, Kurokawa T & Okamura Y
  • Organizer: 42nd Annual Scientific Meeting of the European Symposium of Clinical Investigation 口頭
  • Place of Presentation: Geneve, Switzerland
  • Year and Date: 2008-03-29
• [Presentation] 電位センサードメインの動作 原理と生理機能 (2008)
  • Author(s): 岡村康司
  • Organizer: 第18回細胞電気薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-16
• [Presentation] 電位センサードメインの動作原理と生理機能 (2008)
  • Author(s): 岡村 康司
  • Organizer: 第18回細胞電気薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-16
• [Presentation] Voltage-gated proton channels."Voltage-dependent proton channels:come of age" (2008)
  • Author(s): Okamura.Y.
  • Organizer: Biophysical Society Annual Meeting, 16th IUPAB International Biophysics Congress
  • Place of Presentation: LongBeach,USA.
  • Year and Date: 2008-02-06
• [Presentation] Voltage range for tuning of phosphatase of Ci-VSP as measured by two PIP2-sensors (2008)
  • Author(s): Hossain, M.I., Sakata, S., Murata, Y. & Okamura, Y.
  • Organizer: 52th Biophysical Society Annual Meeting
  • Place of Presentation: Long Beach(USA)(poster)
  • Year and Date: 2008-02-05
• [Presentation] Voltage range for tuning of phosphatase of Ci-VSP as measured by two PIP2-sensors (2008)
  • Author(s): Hossain MI, Sakata S, Murata Y & Okamura Y
  • Organizer: 52th Biophysical Society Annual Meeting, ポスター
  • Place of Presentation: Long Beach, USA
  • Year and Date: 2008-02-05
• [Presentation] Voltage-gated proton channels (2008)
  • Author(s): Okamura Y
  • Organizer: "Voltage-dependent proton channels: come of age", 16th IUPAB International Biophysics Congress, Biophysical Journal, vol. 94, issue 2, pp. 587-588, 口頭
  • Place of Presentation: Long Beach, USA
  • Year and Date: 2008-02-05
• [Presentation] Voltage range for tuning of phospliatase of Ci-VSP as measured by two PIP2-sensors (2008)
  • Author(s): Hossain, M.L, Sakata, S., Murata, Y.&Okamura.Y
  • Organizer: 52th Biophysical Society Annual Meeting
  • Place of Presentation: LongBeach,USA
  • Year and Date: 2008-02-05
• [Presentation] Multimeric nature of voltage-gated proton channels (2008)
  • Author(s): Koch, H.P.;Kurokawa, T., Sasaki, M., Okamura.Y.&Larsson, P
  • Organizer: 52th Biophysical Society Annual:Meeting
  • Place of Presentation: LongBeach,USA
  • Year and Date: 2008-02-05
• [Presentation] 電位依存性プロトンチャネル分子の分布パターンと生理機能 (2008)
  • Author(s): 黒川 竜紀、佐々木 真理、岡村 康司
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] 電位感受性ボスファターゼCi-VSPの酵素活性の脱分極側での計測 (2008)
  • Author(s): 岡村 康司、坂田 宗平、村田 喜理、久保 義弘
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Biochemical characterization of voltage-gated proton channel,VSOP,in phagocytes (2008)
  • Author(s): Okcehi.Y., Sasaki, M., Iwasaki, H., Kurokawa, T.&Okamura.Y
  • Organizer: 42nd Annual Scientific Meeting of the European Symposium of Clinical Investigation
  • Place of Presentation: Geneve,Switzerland
• [Presentation] Is the Hv1 proton channel required for the activity of the NADPH oxidase? (2008)
  • Author(s): Antoun El Chemaly, Okochi, Y., Okamura.Y.&Demaurex, N.
  • Organizer: 42nd Annual Scientific Meeting of the European Symposium of Clinical Investigation
  • Place of Presentation: Geneve,Switzerland
• [Presentation] How do animals utilize signals of membrane potentials? : lessons from two voltage-sensing proteins (2007)
  • Author(s): Okamura Y, Sasaki M, Kurokawa T, Okochi Y, Hossain MI, Iwasaki H, Murata Y & Higashijima S
  • Organizer: The 6th Okazaki Biology Conference "Marine Biology"
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2007-12-04
• [Presentation] How do animals utilize signals of membrane potentials? lessons from two voltage-sensing proteins (2007)
  • Author(s): Okamura Y, Sasaki M, Kurokawa T, Okochi Y, Hossain MI, Iwasaki H, Murata Y & Higashijima S
  • Organizer: the 6th Okazaki Biology Conference: "Marine Biology", 口頭
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2007-12-04
• [Presentation] Mechanisms of voltage-sensor domain proteins and insights into physiological significance (2007)
  • Author(s): Okamura Y
  • Organizer: Annual Meeting of Neuroscience in Chile
  • Place of Presentation: Los Andes, Chile(Plenary Lecture)
  • Year and Date: 2007-09-27
• [Presentation] Mechanisms of voltage-sensor domain proteins and insights into physiological significance (2007)
  • Author(s): Okamura Y
  • Organizer: Plenary Lecture, Annual Meeting of Neuroscience in Chile, 口頭
  • Place of Presentation: Los Andes, Chile
  • Year and Date: 2007-09-27
• [Presentation] Mechanisms of voltage-sensor domain proteins and insights into physiological significance (2007)
  • Author(s): Okamura, Y.
  • Organizer: Plenary Lecture
  • Place of Presentation: LosAndes,Chile
  • Year and Date: 2007-09-27
• [Presentation] Voltage sensor domain proteins (2007)
  • Author(s): Okamura Y
  • Organizer: Ion channel, structure and function, European Biophyics Congress
  • Place of Presentation: London, UK.(Invited talk)
  • Year and Date: 2007-07-16
• [Presentation] Vbltage sensor domain proteins. "Ion channel,structure and function" (2007)
  • Author(s): Okamura, Y.
  • Organizer: European Biophyics Congress
  • Place of Presentation: London,UK
  • Year and Date: 2007-07-16
• [Presentation] 電位依存性ホスファターゼCi-VSPにおける、膜電位感知-酵素活性相関の機構 (2007)
  • Author(s): 村田喜理, 岡村康司
  • Organizer: 第84回日本生理学会大会
  • Place of Presentation: 大阪(Poster)
  • Year and Date: 2007-03-22
• [Presentation] Substrate-dependent structural change of the phosphatasedomain slows the movement of the voltage sensor in 9 voltage-regulatedphosphatases (VSP) (2007)
  • Author(s): Hossain Md I, Higashijima S, Nagayama K, & Okamura Y
  • Organizer: Biophys Society meeting
  • Place of Presentation: Baltimore, USA
• [Presentation] Analysis of avoltage-gated proton channel,VSOP in microglia (2007)
  • Author(s): 大河内 善史、澤田 誠、佐々木 真理、岩崎 広英、岡村 康司
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] カタユウレイボヤ幼生の遊泳運動形成における筋肉nAChRの役割 (2007)
  • Author(s): 西野 敦雄, 岡村 康司
  • Organizer: 第78回日本動物学会
  • Place of Presentation: 弘前
• [Presentation] 電位依存性プロトンチャネルのオリゴマー形成 (2007)
  • Author(s): 黒川 竜紀、大河内 善史、佐々木 真理、岩崎 広英、岡村 康司
  • Organizer: 第30回日本分子生物学会第80回日本生化学会大
  • Place of Presentation: 横浜
• [Presentation] 貪食細胞における電位依存性プロトンチャネルVSOPの機能解析 (2007)
  • Author(s): 大河内 善史、佐々木 真理、黒川 竜紀、岩崎 広英、岡村 康司
  • Organizer: 第30回日本分子生物学会第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] How do animals utilize signals of membrane potentials:lessons from two voltage-sensing proteins (2007)
  • Author(s): Okamura.Y., Sasaki, M., Kurokawa, T., Okochi.Y., Hossain, M.L, Iwasaki, H.Murata, Y.&Higashijima, S
  • Organizer: the 6th Okazaki Biology Conference:"MaxineBiology"
  • Place of Presentation: Okazaki,Japan
• [Presentation] 膜電位シグナルを伝達する新たな膜タンパク質 (2006)
  • Author(s): 岡村康司
  • Organizer: 日本分子生物学フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-08
• [Book] 膜電位情報をイノシトールリン脂質シグナルへ変換する膜タンパク質 VSP, 実験医学増刊「脂質生物学」 (2010)
  • Author(s): 岡村康司
• [Book] 実験医学増刊Vol.28 No.20分子から個体へと深化する脂質生物学 (2010)
  • Author(s): 岡村康司、佐々木雄彦, 横溝岳彦, 竹繩忠臣/編
  • Total Pages: 237
  • Publisher: 羊土社
• [Book] Neural voltage-gated ion channels and novel voltage-sensing proteins. Handbook of Neurochemistry and Molecular Neurobiology (2009)
  • Author(s): Okamura, Y.
  • Total Pages: 632
  • Publisher: Springer
• [Book] Neural voltage-gated ion channels and novel voltage-sensing proteins (2008)
  • Author(s): Okamura.Y
  • Total Pages: 11
  • Publisher: Handbook of Neurochemistry and Molecular Neurobiology
• [Book] Handbook of Neurochem. and Mol. Neurobiology 3rd Ed. Volume1 (Ed. By Mikoshiba K)
  • Author(s): Okamura Y
  • Publisher: (印刷中)
• [Book] 標準生理学 第4版
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/phys2/okamura/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp:80/pub/phys2/tougouseiri/lab_research.htm
• [Patent(Industrial Property Rights)] NOVELION CHANNEL-LIKEPOLYPEPTIDE AND USE THEREOF (2009)
  • Inventor(s): Noriyuki Satoh, Yasushi Okamura, Hirohide Iwasaki, Yoshimichi Murata
  • Industrial Property Rights Holder: 京都大学、自然科学研究機構
  • Filing Date: 2009-09-22
  • Overseas
• [Patent(Industrial Property Rights)] Novel ion channel-like polypeptide and use thereof
  • Inventor(s): 岡村康司, 佐藤矩行, 岩崎広英, 村田喜理
  • Industrial Property Rights Holder: 京都大学, 自然科学研究機構