| Budget Amount *help |
¥72,800,000 (Direct Cost: ¥72,800,000)
Fiscal Year 2010: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2009: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2007: ¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥14,500,000 (Direct Cost: ¥14,500,000)
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| Research Abstract |
Voltage sensing phosphatase gene is conserved in genomes from sea squirt to human. VSP consists of the voltage sensing domain and the cytoplasmic enzyme region that operates as the phosphoinositide phosphatase. Mechanisms of VSP have been examined by integrative approaches including electrophysiology, in vitro phosphatase assay, mathematical modeling analysis and imaging analysis. Results showed that voltage sensor activates PI(4,5)P2 and PIP3 phosphatase activity upon depolarization and this coupling occurs over the entire range of voltage sensitivity of the voltage sensor. We also found that the state of enzyme affects the motions of the voltage sensor suggesting the presence of retrograde interaction from enzyme to the voltage sensor.
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