| Budget Amount *help |
¥187,330,000 (Direct Cost: ¥144,100,000、Indirect Cost: ¥43,230,000)
Fiscal Year 2022: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2021: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2020: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2019: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2018: ¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
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| Outline of Final Research Achievements |
We have succeeded in the development of a new linear-type biomolecular engine by engineering a naturally occurring motor protein, dynein, and various DNA-binding domains. This allowed us to operate more than two types of motors with distinct recognition sequences on a DNA nanotube, making it possible to create a system capable of automatically sorting and integrating cargoes. Moreover, we have successfully engineered naturally occurring rotational biomolecular engines with novel allosteric sites. These sites, created by restoring a lost function during evolution, made possible of the boost and regulation of the rotation. Toward the design of biomolecular engines from scratch, we have developed methods to create new protein building blocks. Furthermore, we have successfully designed proteins that bind ATP and proteins that self-assemble into symmetric oligomers: dimers, pentamers, and hexamers.
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