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High-precision analysis of cell structural stiffness utilizing microscope techniques for single cell measurement and control

Planned Research

Project AreaElucidation of the strategies of mechanical optimization in plants toward the establishment of the bases for sustainable structure system
Project/Area Number 18H05493
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Hosokawa Yoichiroh  奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (20448088)

Co-Investigator(Kenkyū-buntansha) 安國 良平  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (40620612)
三村 徹郎  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (20174120)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000)
Fiscal Year 2022: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2021: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2020: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2019: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2018: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Keywordsキーワード / 原子間力顕微 / フェムト秒レーザー / フォースマッピング / 顕微細胞操作 / 液胞制御 / 原子間力顕微鏡 / 原子間力顕
Outline of Final Research Achievements

Atomic force microscopy (AFM) is used to advance the understanding of the mechanical structure of plant cells. An analytical theory (shell theory) for considering the deflection of the cell wall was constructed with reference to the treatment of structural mechanics in architectural engineering. We devised a method for deriving the elastic modulus of the cell wall and intracellular turgor pressure. By applying this method, we could determine these parameters from the topographic measurement and the indentation test by AFM. It is difficult to estimate them by other methods. This research unraveled the mechanical properties caused by the complex structure of plant cells, and opened a new way to understand the mechanical structure of plants from a microscopic view.

Academic Significance and Societal Importance of the Research Achievements

建築工学における構造力学の理論に基づき、外力による細胞の力学応答と外力が無い場合の膨圧による細胞壁形状のひずみを定式化し、細胞壁の弾性率と膨圧を導く方法を確立することができた。本研究で提案された方法を適用することで、AFMによる細胞形状計測とAFMによるインデンテーション試験による細胞壁のひずみ曲線(フォースカーブ)で決定されるパラメーターを用いて、これまで他の方法で推定が難しかった細胞壁の弾性率と膨圧を導くことができた。本研究により、植物細胞の持つ複雑な構造に起因する力学特性がひも解かれ、植物の力学構造を微視的な観点から理解するための新たな道筋を拓くことができた。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (88 results)

All 2023 2022 2021 2020 2019

All Journal Article (45 results) (of which Int'l Joint Research: 25 results,  Peer Reviewed: 42 results,  Open Access: 20 results) Presentation (43 results) (of which Int'l Joint Research: 6 results,  Invited: 23 results)

  • [Journal Article] Patterned proliferation orients tissue-wide stress to control root vascular symmetry in Arabidopsis2023

    • Author(s)
      Fujiwara M, Imamura M, Matsushita K, Roszak P, Yamashino T, Hosokawa Y, Nakajima K, Fujimoto K, Miyashima S.
    • Journal Title

      Curr Biol.

      Volume: 33 Issue: 5 Pages: 886-893

    • DOI

      10.1016/j.cub.2023.01.036

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microfluidic Separation and Enrichment of Escherichia coli by Size Using Viscoelastic Flows2023

    • Author(s)
      Zhang Tianlong、Cain Amy K.、Semenec Lucie、Liu Ling、Hosokawa Yoichiroh、Inglis David W.、Yalikun Yaxiaer、Li Ming
    • Journal Title

      Analytical Chemistry

      Volume: 95 Issue: 4 Pages: 2561-2569

    • DOI

      10.1021/acs.analchem.2c05084

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser2023

    • Author(s)
      Kiya Ryota、Tang Tao、Tanaka Yo、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1

    • DOI

      10.1038/s41598-022-27229-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multi-Position Measurable Flow Velocity Sensor for Microfluidic Applications2023

    • Author(s)
      Hao Yansheng、Fang Chaoying、Yuan Yapeng、Okano Kazunori、Yasukuni Ryohei、Cheng Shaokoon、Tanaka Yo、Hosokawa Yoichiroh、Yang Yang、Li Ming、Yalikun Yaxiaer
    • Journal Title

      IEEE Sensors Journal

      Volume: 23 Issue: 2 Pages: 1072-1080

    • DOI

      10.1109/jsen.2022.3225637

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Machine learning-based impedance system for real-time recognition of antibiotic-susceptible bacteria with parallel cytometry2022

    • Author(s)
      Tao Tang, Xun Liu, Yapeng Yuan, Ryota Kiya, Tianlong Zhang, Yang Yang, Shiro Suetsugu, Yoichi Yamazaki, Nobutoshi Ota, Koki Yamamoto, Hironari Kamikubo, Yo Tanaka, Ming Li, Yoichiroh Hosokawa, Yaxiaer Yalikun
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 374 Pages: 132698-132698

    • DOI

      10.1016/j.snb.2022.132698

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Parallel Impedance Cytometry for Real-Time Screening of Bacterial Single Cells from Nano- to Microscale2022

    • Author(s)
      Tang Tao、Liu Xun、Yuan Yapeng、Zhang Tianlong、Kiya Ryota、Yang Yang、Yamazaki Yoichi、Kamikubo Hironari、Tanaka Yo、Li Ming、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      ACS Sensors

      Volume: 7 Issue: 12 Pages: 3700-3709

    • DOI

      10.1021/acssensors.2c01351

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span2022

    • Author(s)
      Liu Xun、Tang Tao、Yi Po-Wei、Yuan Yapeng、Lei Cheng、Li Ming、Tanaka Yo、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      Sensors

      Volume: 22 Issue: 20 Pages: 7743-7743

    • DOI

      10.3390/s22207743

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reverse sorting of immortalized megakaryocyte progenitor cell lines (imMKCLs) by femtosecond laser scanning.2022

    • Author(s)
      D Anggraini, W Mubarok, Y Hosokawa, K Eto, S Suetsugu, Y Yalikun.
    • Journal Title

      Applied Physics Express

      Volume: 15 Issue: 10 Pages: 107001-107001

    • DOI

      10.35848/1882-0786/ac8f17

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Femtosecond laser perforation of plant cells for evaluation of cell stiffness2022

    • Author(s)
      Yamasaki Yuki、Tsugawa Satoru、Ito Kenshiro、Okano Kazunori、Hosokawa Yoichiroh
    • Journal Title

      Applied Physics A

      Volume: 128 Issue: 9 Pages: 838-838

    • DOI

      10.1007/s00339-022-05959-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement2022

    • Author(s)
      Tsugawa Satoru、Yamasaki Yuki、Horiguchi Shota、Zhang Tianhao、Muto Takara、Nakaso Yosuke、Ito Kenshiro、Takebayashi Ryu、Okano Kazunori、Akita Eri、Yasukuni Ryohei、Demura Taku、Mimura Tetsuro、Kawaguchi Ken’ichi、Hosokawa Yoichiroh
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 13044-13044

    • DOI

      10.1038/s41598-022-16880-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anhydrobiotic chironomid larval motion-based multi-sensing microdevice for the exploration of survivable locations2022

    • Author(s)
      Yo Tanaka, Doudou Ma, Satoshi Amaya, Yusufu Aishan, Yigang Shen, Shun-ichi Funano, Tao Tang, Yoichiroh Hosokawa, Oleg Gusev, Takashi Okuda, Takahiro Kikawada, Yaxiaer Yalikun
    • Journal Title

      iScience

      Volume: 25 Issue: 8 Pages: 104639-104639

    • DOI

      10.1016/j.isci.2022.104639

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Trihelix transcription factors GTL1 and DF1 prevent aberrant root hair formation in an excess nutrient condition2022

    • Author(s)
      Shibata Michitaro、Favero David S.、Takebayashi Ryu、Takebayashi Arika、Kawamura Ayako、Rymen Bart、Hosokawa Yoichiroh、Sugimoto Keiko
    • Journal Title

      New Phytologist

      Volume: 235 Issue: 4 Pages: 1426-1441

    • DOI

      10.1111/nph.18255

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shape-based separation of drug-treated Escherichia coli using viscoelastic microfluidics2022

    • Author(s)
      Zhang Tianlong、Liu Hangrui、Okano Kazunori、Tang Tao、Inoue Kazuki、Yamazaki Yoichi、Kamikubo Hironari、Cain Amy K.、Tanaka Yo、Inglis David W.、Hosokawa Yoichiroh、Yaxiaer Yalikun、Li Ming
    • Journal Title

      Lab on a Chip

      Volume: 22 Issue: 15 Pages: 2801-2809

    • DOI

      10.1039/d2lc00339b

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Experimental study of intra-ring anatomical variation in Populus alba L. with respect to changes in temperature and day-length conditions.2022

    • Author(s)
      Kei' ichi Baba, Yuko Kurita, Tetsuro Mimura
    • Journal Title

      Forests

      Volume: 13 Issue: 7 Pages: 1151-1159

    • DOI

      10.3390/f13071151

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biogeochemical characteristics of the Hovsgol-Ustilimsk water system in Mongolia and Russia: the effect of environmental factors on dissolved chemical components2022

    • Author(s)
      Hosoda Ko、Murata Takahiro、Mochizuki Akihito、Katano Toshiya、Tanaka Yuji、Mimura Tetsuro、Mitamura Osamu、Nakano Shin-ichi、Sugiyama Yuko、Satoh Yasuhiro、Watanabe Yasunori、Dulmaa Ayuriin、Ayushsuren Chananbaatar、Ganchimeg Darmaa、Drucker Valentin V.、Fialkov Vladimir A.、Sugiyama Masahito
    • Journal Title

      Limnology

      Volume: 23 Issue: 2 Pages: 385-402

    • DOI

      10.1007/s10201-021-00694-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The involvement of AtMKK1 and AtMKK3 in plant-deleterious microbial volatile compounds-induced defense responses2022

    • Author(s)
      Chang Ching-Han、Wang Wu-Guei、Su Pei-Yu、Chen Yu-Shuo、Nguyen Tri-Phuong、Xu Jian、Ohme-Takagi Masaru、Mimura Tetsuro、Hou Ping-Fu、Huang Hao-Jen
    • Journal Title

      Plant Molecular Biology

      Volume: 111 Issue: 1-2 Pages: 21-36

    • DOI

      10.1007/s11103-022-01308-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging MS Analysis in Catharanthus roseus2022

    • Author(s)
      Yamamoto Kotaro、Takahashi Katsutoshi、O’Connor Sarah E.、Mimura Tetsuro
    • Journal Title

      Methods in Molecular Biology

      Volume: 2505 Pages: 33-43

    • DOI

      10.1007/978-1-0716-2349-7_2

    • ISBN
      9781071623480, 9781071623497
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of fluorescent alkaloid metabolism between idioblast and lacticifer cells during leaf development in Catharanthus roseus seedlings2022

    • Author(s)
      Uzaki Mai、Yamamoto Kotaro、Murakami Akio、Fuji Yushiro、Ohnishi Miwa、Ishizaki Kimitsune、Fukaki Hidehiro、Hirai Masami Yokota、Mimura Tetsuro
    • Journal Title

      Journal of Plant Research

      Volume: - Issue: 3 Pages: 473-483

    • DOI

      10.1007/s10265-022-01380-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visualization of phosphorus re-translocation and phosphate transporter expression profiles in a shortened annual cycle system of poplar.2022

    • Author(s)
      Yuko Kurita;Satomi Kanno;Ryohei Sugita;Atsushi Hirose;Miwa Ohnishi;Ayumi Tezuka;Ayumi Deguchi;Kimitsune Ishizaki;Hidehiro Fukaki;Kei'ichi Baba;Atsushi J Nagano;Keitaro Tanoi;Tomoko M Nakanishi;Tetsuro Mimura
    • Journal Title

      Plant, cell & environment

      Volume: 45 Issue: 6 Pages: 1-16

    • DOI

      10.1111/pce.14319

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent advances in microfluidic devices for single-cell cultivation: methods and applications2022

    • Author(s)
      Anggraini Dian、Ota Nobutoshi、Shen Yigang、Tang Tao、Tanaka Yo、Hosokawa Yoichiroh、Li Ming、Yalikun Yaxiaer
    • Journal Title

      Lab on a Chip

      Volume: 22 Issue: 8 Pages: 1438-1468

    • DOI

      10.1039/d1lc01030a

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A gas flow velocity sensor fabricated with femtosecond laser using 4 μm ultra-thin glass sheet2022

    • Author(s)
      Hao Yansheng、Tanaka Yo、Hosokawa Yoichiroh、Li Ming、Yalikun Yaxiaer
    • Journal Title

      Applied Physics Express

      Volume: 15 Issue: 3 Pages: 036502-036502

    • DOI

      10.35848/1882-0786/ac516d

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dual-frequency impedance assays for intracellular components in microalgal cells2022

    • Author(s)
      Tang Tao、Liu Xun、Yuan Yapeng、Kiya Ryota、Shen Yigang、Zhang Tianlong、Suzuki Kengo、Tanaka Yo、Li Ming、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      Lab on a Chip

      Volume: 22 Issue: 3 Pages: 550-559

    • DOI

      10.1039/d1lc00721a

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Impedance-based tracking of the loss of intracellular components in microalgae cells2022

    • Author(s)
      Tang Tao、Liu Xun、Yuan Yapeng、Zhang Tianlong、Kiya Ryota、Suzuki Kengo、Tanaka Yo、Li Ming、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 358 Pages: 131514-131514

    • DOI

      10.1016/j.snb.2022.131514

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improved virus-induced gene silencing allows discovery of a serpentine synthase gene in <i>Catharanthus roseus</i>2021

    • Author(s)
      Yamamoto Kotaro、Grzech Dagny、Koudounas Konstantinos、Stander Emily Amor、Caputi Lorenzo、Mimura Tetsuro、Courdavault Vincent、O’Connor Sarah E.
    • Journal Title

      Plant Physiology

      Volume: 187 Issue: 2 Pages: 846-857

    • DOI

      10.1093/plphys/kiab285

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells2021

    • Author(s)
      Tamako Nishimura*, Takuya Oyama*, Hooi Ting Hu*, Toshifumi Fujioka* et al.(*:equally contributed)
    • Journal Title

      Developmental Cell

      Volume: 56 Issue: 6 Pages: 842-859

    • DOI

      10.1016/j.devcel.2021.02.029

    • NAID

      120007172296

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rotation of Biological Cells: Fundamentals and Applications2021

    • Author(s)
      Tang Tao、Hosokawa Yoichiroh、Hayakawa Takeshi、Tanaka Yo、Li Weihua、Li Ming、Yalikun Yaxiaer
    • Journal Title

      Engineering

      Volume: 6 Pages: 1-28

    • DOI

      10.1016/j.eng.2020.07.031

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microscopic impedance cytometry for quantifying single cell shape2021

    • Author(s)
      Tang Tao、Liu Xun、Kiya Ryota、Shen Yigang、Yuan Yapeng、Zhang Tianlong、Suzuki Kengo、Tanaka Yo、Li Ming、Hosokawa Yoichiroh、Yalikun Yaxiaer
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 193 Pages: 113521-113521

    • DOI

      10.1016/j.bios.2021.113521

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Force measurement of plant cell utilizing atomic force microscopy2021

    • Author(s)
      Yamasaki Yuki、Akita Eri、Okano Kazunori、Hosokawa Yoichiroh
    • Journal Title

      PLANT MORPHOLOGY

      Volume: 33 Issue: 1 Pages: 41-45

    • DOI

      10.5685/plmorphol.33.41

    • ISSN
      0918-9726, 1884-4154
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pulsed laser activated impulse response encoder (PLAIRE): sensitive evaluation of surface cellular stiffness on zebrafish embryos2021

    • Author(s)
      Yasukuni Ryohei、Minamino Daiki、Iino Takanori、Araki Takashi、Takao Kohei、Yamada Sohei、Bessho Yasumasa、Matsui Takaaki、Hosokawa Yoichiroh
    • Journal Title

      Biomedical Optics Express

      Volume: 12 Issue: 3 Pages: 1366-1366

    • DOI

      10.1364/boe.414338

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cooperative Optical Trapping of Polystyrene Microparticle and Protein Forming a Submillimeter Linear Assembly of Microparticle2021

    • Author(s)
      Po-Wei Yi,Wei-Hsiang Chiu,Tetsuhiro Kudo,Teruki Sugiyama,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Shuichi Toyouchi,Hiroshi Masuhara
    • Journal Title

      J. Phys. Chem. C

      Volume: 125 Issue: 34 Pages: 18988-18999

    • DOI

      10.1021/acs.jpcc.1c05796

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Focusing of Particles in a Microchannel with Laser Engraved Groove Arrays2021

    • Author(s)
      Zhang Tianlong、Shen Yigang、Kiya Ryota、Anggraini Dian、Tang Tao、Uno Hanaka、Okano Kazunori、Tanaka Yo、Hosokawa Yoichiroh、Li Ming、Yalikun Yaxiaer
    • Journal Title

      Biosensors

      Volume: 11 Issue: 8 Pages: 263-263

    • DOI

      10.3390/bios11080263

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers2021

    • Author(s)
      Zhang Tianlong、Namoto Misuzu、Okano Kazunori、Akita Eri、Teranishi Norihiro、Tang Tao、Anggraini Dian、Hao Yansheng、Tanaka Yo、Inglis David、Yalikun Yaxiaer、Li Ming、Hosokawa Yoichiroh
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 1652-1652

    • DOI

      10.1038/s41598-021-81190-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In situ measurement of cell stiffness of Arabidopsis roots growing on a glass micropillar support by atomic force microscopy2020

    • Author(s)
      Akita Eri、Yalikun Yaxiaer、Okano Kazunori、Yamasaki Yuki、Ohtani Misato、Tanaka Yo、Demura Taku、Hosokawa Yoichiroh
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 4 Pages: 417-422

    • DOI

      10.5511/plantbiotechnology.20.1016a

    • NAID

      130007960233

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-12-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells2020

    • Author(s)
      Yoneda Arata、Ohtani Misato、Katagiri Daisuke、Hosokawa Yoichiroh、Demura Taku
    • Journal Title

      Plants

      Volume: 9 Issue: 5 Pages: 604-604

    • DOI

      10.3390/plants9050604

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photoinjection of fluorescent nanoparticles into intact plant cells using femtosecond laser amplifier2020

    • Author(s)
      Rukmana Taufiq Indra、Moran Gabriela、Meallet-Renault Rachel、Clavier Gilles、Kunieda Tadashi、Ohtani Misato、Demura Taku、Yasukuni Ryohei、Hosokawa Yoichiroh
    • Journal Title

      APL Photonics

      Volume: 5 Issue: 6 Pages: 066104-066104

    • DOI

      10.1063/5.0001687

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Direct observation of nanoparticle diffusion in cytoplasm of single plant cells realized by photoinjection with femtosecond laser amplifier2020

    • Author(s)
      Rukmana Taufiq Indra、Yasukuni Ryohei、Moran Gabriela、Meallet-Renault Rachel、Clavier Gilles、Kunieda Tadashi、Ohtani Misato、Demura Taku、Hosokawa Yoichiroh
    • Journal Title

      Applied Physics Express

      Volume: 13 Issue: 11 Pages: 117002-117002

    • DOI

      10.35848/1882-0786/abc490

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mechanical properties of single cells: Measurement methods and applications2020

    • Author(s)
      Hao Yansheng、Cheng Shaokoon、Tanaka Yo、Hosokawa Yoichiroh、Yalikun Yaxiaer、Li Ming
    • Journal Title

      Biotechnology Advances

      Volume: 45 Pages: 107648-107648

    • DOI

      10.1016/j.biotechadv.2020.107648

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation2020

    • Author(s)
      Okano Kazunori、Wang Chung-Han、Hong Zhen-Yi、Hosokawa Yoichiroh、Liau Ian
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 24 Pages: 100818-100818

    • DOI

      10.1016/j.bbrep.2020.100818

    • NAID

      120006959887

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Calcium dynamics during trap closure visualized in transgenic Venus flytrap2020

    • Author(s)
      Suda Hiraku、Mano Hiroaki、Toyota Masatsugu、Fukushima Kenji、Mimura Tetsuro、Tsutsui Izuo、Hedrich Rainer、Tamada Yosuke、Hasebe Mitsuyasu
    • Journal Title

      Nature Plants

      Volume: 6 Issue: 10 Pages: 1219-1224

    • DOI

      10.1038/s41477-020-00773-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visualization of seasonal shifts of phosphorus re-translocation and overwintering recycling in a shortened annual cycle system of the deciduous poplar tree (Populus alba L.)2020

    • Author(s)
      Kurita Yuko、Kanno Satomi、Sugita Ryohei、Hirose Atsushi、Ohnishi Miwa、Ishizaki Kimitsune、Fukaki Hidehiro、Tanoi Keitaro、Nakanishi Tomoko M.、Baba Kei’ichi、Mimura Tetsuro
    • Journal Title

      BioRxiv

      Volume: 10.1101

    • DOI

      10.1101/2020.04.12.038562

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Single-cell metabolome analysis in plants2020

    • Author(s)
      山本 浩太郎、三村 徹郎
    • Journal Title

      Regulation of Plant Growth & Development

      Volume: 55 Issue: 2 Pages: 100-104

    • DOI

      10.18978/jscrp.55.2_100

    • NAID

      130008131350

    • ISSN
      1346-5406, 2189-6305
    • Related Report
      2020 Annual Research Report
  • [Journal Article] High-speed micro-particle manipulation in a microfluidic chip by directional femtosecond laser impulse2019

    • Author(s)
      Zhen-Yi Hong, Kazunori Okano, Dino Di Carlo, Katsunori Tanaka, Yaxiaer Yalikun and Yoichiroh Hosokawa
    • Journal Title

      Sensors and Actuators A: Physical

      Volume: 297 Pages: 111566-111566

    • DOI

      10.1016/j.sna.2019.111566

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Applications of femtosecond laser-induced impulse to cell research2019

    • Author(s)
      Yoichiroh Hosokawa
    • Journal Title

      JJAP invited review

      Volume: Vol.58, No.11 Issue: 11 Pages: 110102-110102

    • DOI

      10.7567/1347-4065/ab4749

    • NAID

      210000157177

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier2019

    • Author(s)
      Taufiq Indra Rukmana, Gabriela Moran, Rachel Meallet-Renault, Misato Ohtani, Taku Demura
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 17530-17530

    • DOI

      10.1038/s41598-019-54124-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] フェムト秒レーザーを用いたTobacco BY植物細胞への巨大高分子導入2019

    • Author(s)
      安國 良平, Rukmana Taufiq Indra, 大谷 美沙都, 出村 拓, 細川 陽一郎
    • Journal Title

      光・量子デバイス研究会資料

      Volume: - Pages: 17-19

    • Related Report
      2018 Annual Research Report
  • [Presentation] フェムト秒レーザーを駆使した顕微鏡下での細胞・生体材料の操作と評価2023

    • Author(s)
      細川陽一郎
    • Organizer
      第1回 電気学会 調査専門委員会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Turning serendipity into planned happenstance with the closest country, Taiwan2023

    • Author(s)
      Yoichiroh Hosokawa
    • Organizer
      奈良先端科学技術大学院大学 Taiwan Interdisciplinary Nano/Bio in Kansai
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Short impression about educational and research situation of basic plant biology in NCKU and Academia Sinica at Tainan2023

    • Author(s)
      Tetsuro Mimura
    • Organizer
      奈良先端科学技術大学院大学 Taiwan Interdisciplinary Nano/Bio in Kansai
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 薬用植物ニチニチソウは、なぜ・どうやって薬を作るのか2023

    • Author(s)
      三村徹郎
    • Organizer
      [大隅基礎科学創成財団]第6期第5回(2/10)創発セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] リンと生命2022

    • Author(s)
      三村徹郎
    • Organizer
      第3回宇宙と生命の勉強会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Evolution of Phosphate Transporters and History of Phosphorus Transport Research2022

    • Author(s)
      Tetsuro Mimura
    • Organizer
      日本植物学会第86会大会 JPR international symposium
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] リンと生命22022

    • Author(s)
      三村徹郎
    • Organizer
      第4回宇宙と生命の勉強会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Cell-specific analysis of specialized metabolism in medicinal plants2022

    • Author(s)
      Tetsuro Mimura
    • Organizer
      Invited lecture 台湾國立中山大生物科學系
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] フェムト秒レーザー誘起衝撃力と原子間力顕微鏡を駆使した単一細胞の力学状態計測2022

    • Author(s)
      細川 陽一郎
    • Organizer
      第4回 メカノバイオロジー 分科会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 植物の生理を支える水と圧力2022

    • Author(s)
      三村徹郎
    • Organizer
      複雑系生命秩序懇談会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Applications of femtosecond laser amplifier to microprocesses for biological cells2021

    • Author(s)
      細川 陽一郎
    • Organizer
      34th International Microprocesses and Nanotechnology Conference (MNC2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Application of femtsoecond laser impulse for evaluation of cell adhesion2021

    • Author(s)
      細川 陽一郎
    • Organizer
      NANOSYNERGETICS Workshop
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Chemical and sub-cellular approach for plant cell functions and its application2021

    • Author(s)
      三村徹郎
    • Organizer
      Invited lecture, 台灣國立陽明交通大學
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Phosphorus status in plant life ?2021

    • Author(s)
      三村徹郎
    • Organizer
      Invited seminar, 台灣 Academia Sinica
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 超短パルスレーザーが拓く単一細胞の操作・計測技術2021

    • Author(s)
      細川陽一郎
    • Organizer
      レーザー学会学術講演会第41回年次大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Dynamics of vacuolar function in plant cell2021

    • Author(s)
      Tetsuro Mimura
    • Organizer
      Lecture on Plant Cell Biology and Physiology at Department of Agronomy and Horticulture, Faculty of Agriculture IPB University, Bogor, Indonesia
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single cell manipulations utilizing femtosecond laser impulse and the physics2020

    • Author(s)
      細川陽一郎
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] マイクロデバイス上でのAFM計測による成長する植物細胞の力学状態の評価2020

    • Author(s)
      秋田絵理
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] フェムト秒レーザー誘起衝撃力を利用した細胞間の力学相互作用の解明2020

    • Author(s)
      細川陽一郎
    • Organizer
      レーザー学会学術講演会第40回年次大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] フェムト秒レーザーと原子間力顕微鏡を用いた単一植物細胞の力学計測2020

    • Author(s)
      竹林竜,秋田絵里,岡野和宣,安國良平,細川陽一郎
    • Organizer
      レーザー学会学術講演会第40回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] フェムト秒レーザーを用いた植物細胞へのナノ粒子導入2020

    • Author(s)
      安國良平
    • Organizer
      レーザー学会学術講演会第40回年次大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 顕微技術を駆使した計測と制御による細胞構造のしなやかさの高精度解析2019

    • Author(s)
      細川陽一郎
    • Organizer
      新学術領域研究「植物構造オプト」第2回班会議
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Femtosecond laser photoporation of fluorogenic peptide aptamers for highly sensitive imaging of polysaccharides in Euglena gracilis2019

    • Author(s)
      Takanori Maeno, Takanori Uzawa, Izumi Kono, Kazunori Okano, Takanori Iino, Taro Ogawa, Osamu Iwata, Takuro Ito, Kengo Suzuki, Keisuke Goda, Yoichiroh Hosokawa
    • Organizer
      The 8th International Congress on Laser Advanced Materials Processing
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Acoustic cell arrangement in micro-fluidic chip for cells sorting by femtosecond laser-induced impulsive force2019

    • Author(s)
      Yusuke Ajikawa, Tomoyuki Suzuki, Yalikun Yaxiaer, Yoichiroh Hosokawa
    • Organizer
      M&BE2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Particle Arrangement in Microfluidic Chip by Additive Femtosecond Laser Processing in the Channel2019

    • Author(s)
      Y. Yalikun, Z. Tianlong, C. Fang, M. Namoto, Y. Hosokawa
    • Organizer
      15th International Conference on Laser Ablation
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Femtosecond Laser-induced Impulsive Force in Applications for Cell Research2019

    • Author(s)
      Yoichiroh Hosokawa
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] A Transparent Flow Velocity Sensor of Ultra-thin Glass Sheet Fabricated by Femtosecond Laser Processing2019

    • Author(s)
      Yansheng Hao, Chaoying Fang, Ming Li, Yaxiaer Yalikun, Yoichiroh Hosokawa
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Femtosecond Laser-processed Pattern on Glass Substrate for Focusing Microparticles in Fluid2019

    • Author(s)
      Tianlong Zhang, Misuzu Namoto, Chaoying Fang, Ming Li, Yalikun Yaxiaer, and Yoichiroh Hosokawa
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ガラス製マイクロデバイスを用いた植物組織のAFM力学計測2019

    • Author(s)
      秋田絵理, 竹林竜, 岡野和宣, Yalikun Yaxiaer, 岸田佳祐, 國枝正, 出村拓, 澤進一郎, 細川陽一郎
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 原子間力顕微鏡とフェムト秒レーザーを用いた細胞組織の力学特性解析2019

    • Author(s)
      竹林竜,秋田絵理,岡野和宣,安國良平,細川陽一郎
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Photoinjection of Flourescence Nanoparticles into Plant Cells Using Femtosecond Laser Amplifier with Additional Enzyme Treatment2019

    • Author(s)
      Taufiq Indra Rukmana, Gabriela Moran, Rachel Meallet-Renault, Misato Ohtani,Taku Demura, Ryohei Yasukuni, Yoichiroh Hosokawa
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] VND7木部道管細胞誘導系を用いたシロイヌナズナの二次細胞壁パターン形成制御の解析2019

    • Author(s)
      岸田 佳祐, 國枝 正, 津川 暁 , 細川 陽一郎 , 出村 拓
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] AFM力学計測のための新規ガラスマイクロデバイスの開発2019

    • Author(s)
      秋田絵理, 竹林竜, 岡野和宣, Yalikun Yaxiaer, 岸田佳祐, 國枝正, 大谷美沙都,出村拓, 澤進一郎, 細川陽一郎
    • Organizer
      植物構造オプト第1回若手の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] フェムト秒レーザーアブレーション法とAFMを組み合わせた単一植物細胞の力学計測装置の開発2019

    • Author(s)
      竹林竜,秋田絵理,岡野和宣,細川陽一郎
    • Organizer
      植物構造オプト第1回若手の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] フェムト秒レーザーを用いた単一植物細胞への巨大分子導入2019

    • Author(s)
      上田勇真, Taufiq Indra Rukmana, 岡野和宣, 安國良平, 細川陽一郎, 出村拓, 大谷美沙都
    • Organizer
      植物構造オプト第1回若手の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マイクロデバイス中でのフェムト秒レーザー誘起衝撃力の数値解析2019

    • Author(s)
      山本将也, 阿路川雄介, 寺西教裕, 安國良平, 細川陽一郎
    • Organizer
      植物構造オプト第1回若手の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナ木部道管細胞誘導系によって形成される二次細胞壁の力学的解析2019

    • Author(s)
      岸田佳祐、國枝正、津川暁、秋田絵理、岡野和宣、細川陽一郎、出村拓
    • Organizer
      植物構造オプト第1回若手の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] AFM Force Measurement of Arabidopsis Utilizing Femtosecond Laser Processing and Glass Micro-Device2019

    • Author(s)
      細川陽一郎
    • Organizer
      熊本大学ミニシンポ "Strategies of mechanical optimization in plants "
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Imaging of elasticity of Arabidopsis root cells by atomic force microscopy2019

    • Author(s)
      Ryu Takebayashi, Eri Akita, Kazunori Okano, Ryohei Yasukuni, Misato Ohtani, Taku Demura and Yoichiroh Hosokawa
    • Organizer
      陽明大学セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] Force mapping of Arabidopsis root cells by atomic force microscop2019

    • Author(s)
      Ryu Takebayashi, Eri Akita, Kazunori Okano, Ryohei Yasukuni, Misato Ohtani, Taku Demura and Yoichiroh Hosokawa
    • Organizer
      2019 Annual Meeting of the Physical Society of Taiwan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 原子間力顕微鏡による植物細胞表面の弾性分布の観察2019

    • Author(s)
      竹林 竜, 秋田 絵里, 岡野 和宣, 安國 良平, 大谷 美沙都, 出村 拓, 細川 陽一郎
    • Organizer
      応用物理学会関西支部 平成30年度第3回支部講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] フェムト秒レーザーを用いたTobacco BY植物細胞への巨大高分子導入2019

    • Author(s)
      安國 良平, Rukmana Taufiq Indra, 大谷 美沙都, 出村 拓, 細川 陽一郎
    • Organizer
      光・量子デバイス研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Detection and manipulation of single plant cells by fs laser and AFM2019

    • Author(s)
      Yoichiroh Hosokawa
    • Organizer
      ISMO-NAIST Symposium
    • Related Report
      2018 Annual Research Report

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Published: 2018-07-20   Modified: 2024-01-30  

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