| Budget Amount *help |
¥78,520,000 (Direct Cost: ¥60,400,000、Indirect Cost: ¥18,120,000)
Fiscal Year 2022: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2021: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2020: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2019: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Outline of Final Research Achievements |
This study focused on the molecular functions of plant extracellular proteoglycans, arabinogalactan-proteins (AGPs) and pectin to clarify how non-cellulosic polysaccharides contribute to mechanical properties of plants. We generated transgenic Arabidopsis expressing fungal glycoside hydrolases specific to the glycan moieties of AGP. The degradation of AGPs changed the mechanical properties of cell walls causing severe dwarf phenotypes in Arabidopsis seedlings. Cell wall analysis suggested that AGPs are required for normal cellulose synthesis. We also identified rhamnogalacturonan (RG)-I L-rhamnosyltransferases, RRTs, in Arabidopsis. In addition, arabinan side chains of RG-I was shown to affect mechanical properties of cell wall via cell adhesion and cell shape regulation.
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