| Budget Amount *help |
¥87,620,000 (Direct Cost: ¥67,400,000、Indirect Cost: ¥20,220,000)
Fiscal Year 2022: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2021: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2020: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2019: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2018: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
We designed ubiquitin derivatives with a photodegradable protecting group on the lysine residue used for polymerization. Using this ubiquitin derivative, we were able to control the length and branching position of ubiquitin by repeated enzymatic elongation and deprotection of the protecting group by light. Control of branching position was achieved without separation of intermediates, allowing one-pot synthesis of K63 triubiquitin chains and K63/K48 heterotypic tetraubiquitin chains with defined branching positions. This study provides a chemical platform for the efficient construction of long polyubiquitin chains with defined branching structures and will facilitate the elucidation of the essential relationship between function and structure of heterotypic chains that has been overlooked.
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