| Budget Amount *help |
¥235,690,000 (Direct Cost: ¥181,300,000、Indirect Cost: ¥54,390,000)
Fiscal Year 2022: ¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2021: ¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2020: ¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2019: ¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2018: ¥51,090,000 (Direct Cost: ¥39,300,000、Indirect Cost: ¥11,790,000)
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| Outline of Final Research Achievements |
This research aimed to elucidate the function of chromatin structure through histone modifications from the perspective of transcriptional activation potential. Through live-cell imaging of zebrafish embryos, we revealed that histone acetylation enhances the potential for transcriptional activation. We also developed genetically encoded single-stranded variable region antibody probes that specifically recognize phosphorylation of RNA polymerase II at the transcription initiation and elongation stages. Based on the distinct localization and dynamics of transcription initiation and elongation foci in living cells, a model of transcription organization was proposed. In addition, we have developed low-input epigenome analysis techniques.
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