| Budget Amount *help |
¥276,900,000 (Direct Cost: ¥213,000,000、Indirect Cost: ¥63,900,000)
Fiscal Year 2022: ¥51,350,000 (Direct Cost: ¥39,500,000、Indirect Cost: ¥11,850,000)
Fiscal Year 2021: ¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2020: ¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000)
Fiscal Year 2019: ¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000)
Fiscal Year 2018: ¥76,440,000 (Direct Cost: ¥58,800,000、Indirect Cost: ¥17,640,000)
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| Outline of Final Research Achievements |
By comparing the differentiation process of mouse oocytes in vivo and in vitro culture system, we found that hypoxia and mechanical stress conditions are necessary for the maintenance of primordial follicles. When these conditions were reproduced in the culture system, the quiescent state of primordial follicles was, which was not possible in the previous in vitro culture systems. In addition, we established culture system that produces gonadal somatic cell-like cells from mouse pluripotent stem cells. These induced cells were capable of fully supporting oocyte growth and provided oocytes with competence of fertilization and full-term development. Finally, we established culture systems that produce primordial germ cell-like cells from pluripotent stem cells of marmoset, bovine, and northen white rhinoceros.
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