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Non-invasive prediction of gamete integrity

Planned Research

Project AreaEnsuring integrity in gametogenesis
Project/Area Number 18H05550
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tsukuba

Principal Investigator

Yawata Yutaka  筑波大学, 生命環境系, 准教授 (10586457)

Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥94,900,000 (Direct Cost: ¥73,000,000、Indirect Cost: ¥21,900,000)
Fiscal Year 2022: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Keywords配偶子 / 自家蛍光 / CRIF / 共焦点顕微鏡 / 機械学習 / 一細胞解析 / 卵子 / 体外培養 / 自家蛍光解析 / 非破壊評価 / in vitro gametogenesis / 非破壊分析 / 内在性蛍光 / 可視化技術 / インテグリティ / 顕微鏡
Outline of Final Research Achievements

1)Fertility prediction for mouse egges:A population of mouse eggs, regardless of grown under in vivo or in vitro conditions, contains infertile individuals. In the present study, we developed a non-inavasive method to detect the infertile eggs by innate fluorescence signature analysis. 2)Tag-free 3D visualization of mouse testis : A mouse testis consists of germ cells and feeder cells. In the present study, we found the innate fluorescence signature characteristic to each cell types and developed a tag-free 3D visualization of testis structure based on innate fluorescence. 3)Tag-free cell sorting technology: We developed a tag-free method to enrich specific types of cell from a mixed population using a combination of single-cell innate fluoresce analysis and cell-sorting technology.

Academic Significance and Societal Importance of the Research Achievements

1)マウス卵子の受精能予測については、受精能のない卵子を非破壊的に見分けることで、将来的に体外受精効率の向上に資する可能性がある。2)マウス精巣の構造可視化については、手を加えない精巣組織構造の解析が可能となることで、精巣の機能や発達過程の理解に資することが期待される。3)魚類生殖細胞の分取では、代理親魚技術などの改善に役立つことが期待できるだけでなく、非モデル生物における細胞分取技術を確立したところに意義があるといえる。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (4 results)

All 2019 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Intra- and Interspecies Variability of Single-Cell Innate Fluorescence Signature of Microbial Cell2019

    • Author(s)
      Yawata Yutaka、Kiyokawa Tatsunori、Kawamura Yuhki、Hirayama Tomohiro、Takabe Kyosuke、Nomura Nobuhiko
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85 Issue: 18 Pages: 1-10

    • DOI

      10.1128/aem.00608-19

    • NAID

      120007133099

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Single-Cell Innate Fluorescence Analysis by Confocal Microspectroscopy2019

    • Author(s)
      Yutaka Yawata
    • Organizer
      TSB2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Cell Innate Fluorescence Analysis by Confocal Microspectroscopy2019

    • Author(s)
      Yutaka Yawata 、Kyosuke Takabe、Nobuhiko Nomura
    • Organizer
      - 日本顕微鏡学会第62回シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 八幡研究室

    • URL

      https://yawatalab.jp

    • Related Report
      2019 Annual Research Report

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Published: 2018-07-20   Modified: 2025-01-30  

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