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Structure and function relationship of enzymes studied by single-molecule spectroscopy at liquid-helium temperature

Planned Research

Project AreaMolecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
Project/Area Number 19056011
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTokyo Institute of Technology

Principal Investigator

MATSUSHITA Michio  東京工業大学, 大学院・理工学研究科, 准教授 (80260032)

Co-Investigator(Kenkyū-buntansha) 藤芳 暁  東京工業大学, 大学院・理工学研究科, 助教 (70371705)
Co-Investigator(Renkei-kenkyūsha) FUJIYOSHI Satoru  東京工業大学, 理工学研究科, 助教 (70371705)
Project Period (FY) 2007 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥35,000,000 (Direct Cost: ¥35,000,000)
Fiscal Year 2011: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2010: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2009: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2008: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2007: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywords単一分子分光 / タンパク質 / 分光 / 低温 / 構造機能相関 / 一分子 / タンパク / 分子 / 一分子計測
Research Abstract

Physical and chemical properties of enzyme can be studied using a homogeneous solution of a purified sample of enzyme. However, in living cells, individual protein molecules of enzyme are in different locations to experience different environments, so that they function differently. For understanding enzymatic reactions on the basis of molecular science, observation of individual molecules is essential. In this study we realized spectroscopy of visible-fluorescent single protein molecules.

Report

(7 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (49 results)

All 2012 2011 2010 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 10 results) Presentation (27 results) Remarks (1 results)

  • [Journal Article] Single-component reflecting objective for low-temperature imaging and spectroscopy of single nano objects2011

    • Author(s)
      M. Fujiwara, S. Fujiyoshi, M. Matsushita
    • Journal Title

      Physics Procedia

      Volume: 13 Pages: 38-38

    • Related Report
      2011 Final Research Report
  • [Journal Article] Single-protein study of photoresistance of pigment-protein complex in lipid bilayer2011

    • Author(s)
      D. Uchiyama, H. Hoshino, K. Otomo, T. Kato, K. Onda, A. Watanabe, H. Oikawa, S. Fujiyoshi, M. Matsushita, M. Nango, N. Watanabe, A. Sumino, T. Dewa
    • Journal Title

      Chem. Phys. Lett.

      Volume: 511 Pages: 135-135

    • Related Report
      2011 Final Research Report
  • [Journal Article] Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K2011

    • Author(s)
      D. Uchiyama, H. Oikawa, K. Otomo, M. Nango, T. Dewa, S. Fujiyoshi, M. Matsushita
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 13 Pages: 11615-11615

    • Related Report
      2011 Final Research Report
  • [Journal Article] Structural Change of a Cofactor Binding Site of Flavoprotein Detected by Single-Protein Fluorescence Spectroscopy at 1.5 K2011

    • Author(s)
      S. Fujiyoshi, M. Hirano, M. Matsushita, M. Iseki, M. Watanabe
    • Journal Title

      Phys. Rev. Lett.

      Volume: 106 Pages: 78101-78101

    • Related Report
      2011 Final Research Report
  • [Journal Article] タンパク質の構造変化と単一分子分光2011

    • Author(s)
      小井川浩之、藤芳暁、出羽毅久、南後守、松下道雄
    • Journal Title

      高分子

      Volume: 60 (2) Pages: 80-81

    • NAID

      10027789907

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 K2011

    • Author(s)
      S.Fujiyoshi, M.Hirano, M.Matsushita, M.Iseki, M.Watanabe
    • Journal Title

      Phys.Rev.Lett.

      Volume: 106

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vibrational Microspectroscopy of Single Proteins2010

    • Author(s)
      S. Fujiyoshi, Y. Furuya, M. Iseki, M. Watanabe, M. Matsushita
    • Journal Title

      J. Phys. Chem. Lett.

      Volume: 1 Pages: 2541-45

    • Related Report
      2011 Final Research Report
  • [Journal Article] Vibrational microspectroscopy of single proteins2010

    • Author(s)
      S.Fujiyoshi, Y.Furuya, M.Iseki, M.Watanabe, M.Matsushita
    • Journal Title

      J.Phys.Chem.Lett.

      Volume: 1 Pages: 2541-2545

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      M. Fujiwara, S. Fujiyoshi, and M. Matsushita
    • Journal Title

      J. Opt. Soc. Am. B

      Volume: 26 Pages: 1395-99

    • Related Report
      2011 Final Research Report
  • [Journal Article] Single-component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      Masanori Fujiwara, Satoru Fujiyoshi, Michio Matsushita
    • Journal Title

      J. Opt. Soc. Am. B 26(7)

      Pages: 1395-99

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Single-component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature2009

    • Author(s)
      Masanori Fujiwara, Satoru Fujiyoshi, Michio Matsushita
    • Journal Title

      Journal of Optical Society of America B 26

      Pages: 1395-1399

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visible fluorescence spectroscopy of single proteins at liquid-helium temperature2008

    • Author(s)
      S. Fujiyoshi, M. Fujiwara, and M. Matsushita
    • Journal Title

      Phys. Rev. Lett.

      Volume: 100 Pages: 168101-168101

    • Related Report
      2011 Final Research Report
  • [Journal Article] How deep is the potential well confining a protein in a specific conformation? A single-molecule study on temperature dependence of conformational change between 5 and 18 K2008

    • Author(s)
      H. Oikawa, S. Fujiyoshi, T. Dewa, M. Nango, and M. Matsushita
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 130 Pages: 4580-81

    • Related Report
      2011 Final Research Report
  • [Journal Article] 光合成アンテナ複合体の単一分子分光2008

    • Author(s)
      松下道雄
    • Journal Title

      生物物理

      Volume: 48 (1) Pages: 35-40

    • NAID

      110006571381

    • Related Report
      2011 Final Research Report
  • [Journal Article] Visible Fluorescence Spectroscopy of Single Proteins at Liquid-Helium Temperature2008

    • Author(s)
      Satoru Fujiyoshi, Masanori Fujiwara, Michio Matsushita
    • Journal Title

      Phys. Rev. Lett. 100(16)

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] How Deep Is the Potential Well Confining a Protein in a Specific Conformation? A Single-Molecule Study on Temperature Dependence of Conformational Change between 5 and 18 K2008

    • Author(s)
      Hiroyuki Oikawa, Satoru Fujiyoshi, Takehisa Dewa, Mamoru Nango, Michio Matsushita
    • Journal Title

      J. Am. Chem. Soc. (Communication) 130(14)

      Pages: 4580-4581

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] How deep is the potential well confining a protein in a specific conformation? A single-molecule study on temperature dependence of conformational change between 5 and 18 K2008

    • Author(s)
      H. Oikawa, S. Fujiyoshi, T. Dewa, M. Nango, and M. Matsushita
    • Journal Title

      The Journal of American Chemical Society 130

      Pages: 4580-4581

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visible fluorescence spectroscopy of single proteins at liquid-helium temperature2008

    • Author(s)
      S. Fujiyoshi, M. Fujiwara, and M. Matsushita
    • Journal Title

      Physical Review Letters 100

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成アンテナ複合体の単一分子分光2008

    • Author(s)
      松下 道雄
    • Journal Title

      生物物理 48

      Pages: 3540-3540

    • NAID

      110006571381

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-component reflecting objective for low-temperature spectroscopy in the entire visible region2007

    • Author(s)
      S. Fujiyoshi, M. Fujiwara, C. Kim, M. Matsushita, A. M. van Oijen, and J. Schmidt
    • Journal Title

      App. Phys. Lett.

      Volume: 91 Pages: 51125-51125

    • Related Report
      2011 Final Research Report
  • [Journal Article] Single-component reflecting objective for low-temperature spectroscopy in the entire visible region2007

    • Author(s)
      Satoru Fujiyoshi, Masanori Fujiwara, Changman Kim, Michio Matsushita, Antoine M. van Oijen, Jan Schmidt
    • Journal Title

      App. Phys. Lett. 91(5)

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] 温度1.5Kの単一分子分光による可視蛍光タンパク質内のプロトン移動を伴った構造変化の観測2012

    • Author(s)
      藤原正規、平野充遥、渡辺正勝、伊関峰生、藤芳暁、松下道雄
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      日吉
    • Related Report
      2011 Final Research Report
  • [Presentation] 中赤外光が誘起するタンパク質一分子の色素結合部位の構造変化2012

    • Author(s)
      大友康平、出羽毅久、松下道雄、藤芳暁
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      日吉
    • Related Report
      2011 Final Research Report
  • [Presentation] 温度数ケルビンのタンパク質1分子分光装置の機械的安定性の評価2011

    • Author(s)
      日野原拓也、濱田裕紀、松下道雄、藤芳暁
    • Organizer
      第5回分子科学討論会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 数Kにおける単一タンパク質分光を用いた可視蛍光タンパク質内のプロトン移動を伴った構造変化の観測2011

    • Author(s)
      藤原正規、平野充遥、渡辺正勝、伊関峰生、藤芳暁、松下道雄
    • Organizer
      第5回分子科学討論会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] Single-molecule spectroscopy of bacterial photosynthetic antenna complexes at liquid-helium temperature2010

    • Author(s)
      Michio Matsushita
    • Organizer
      70th Okazaki international conference on molecular mechanism of photosynthetic energy conversion
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2010-12-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Single-molecule spectroscopy of bacterial photosynthetic antenna complexes at liquid-helium temperature2010

    • Author(s)
      M. Matsushita
    • Organizer
      The 70th Okazaki International Conference on "Molecular mechanism of photosynthetic energy conversion: the present research and future prospects"
    • Place of Presentation
      Okazaki, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 光合成アンテナ複合体の中赤外吸収スペクトルの一分子観測2010

    • Author(s)
      大友康平、出羽毅久、南後守、渡辺正勝、松下道雄、藤芳暁
    • Organizer
      第4回分子科学討論会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] 1.5Kにおける単一色素分子の蛍光スペクトルの時間変化の起源探究2010

    • Author(s)
      島内明理、櫻井敦教、平野充遥、藤芳暁、松下道雄
    • Organizer
      日本物理学会第65回年次大会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] Spectroscopy of single proteins at liquid-helium temperature2010

    • Author(s)
      M. Matsushita
    • Organizer
      Symposium on "Physics and Chemistry of Coherently Controlled Quantum Systems"
    • Place of Presentation
      Inuyama, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 低温下における単一タンパク質分光のための反射対物レンズの開発2009

    • Author(s)
      藤原正規、藤芳暁、松下道雄
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report 2009 Self-evaluation Report
  • [Presentation] 光活性化アデニル酸シクラーゼの単一タンパク質分光2009

    • Author(s)
      藤芳暁、平野充遥、松下道雄、伊関峰夫、渡辺正勝
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] 低温の単一タンパク質分光の励起過程の検討2008

    • Author(s)
      藤芳暁、平野充遙、古屋陽、藤原正規、松下道雄、伊関峯生、渡辺正勝
    • Organizer
      分子科学討論会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 1. 5 Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規、藤芳暁、松下道雄
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      東大阪
    • Year and Date
      2008-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 顕微分光による光合成アンテナ複合体間のエネルギー移動の研究2008

    • Author(s)
      古屋陽、内山大輔、藤芳暁、松下道雄、末森良春、出羽毅久、南後守
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      近畿大学木部キャンパス
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 1.5 Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規、藤芳暁、松下道雄
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      近畿大学木部キャンパス
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 二光子蛍光による低温の単一タンパク質分光2008

    • Author(s)
      藤芳暁、松下道雄
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2008-03-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 低温の単一タンパク質分光の励起過程の検討2008

    • Author(s)
      藤芳暁、平野充遥、古屋陽、藤原正規、松下道雄、伊関峰夫、渡辺正勝
    • Organizer
      第2回分子科学討論会
    • Place of Presentation
      福岡
    • Related Report
      2011 Final Research Report 2009 Self-evaluation Report
  • [Presentation] 顕微分光による光合成アンテナ複合体間のエネルギー移動の研究2008

    • Author(s)
      古屋内山大輔、藤芳暁、松下道雄、末守良春、出羽毅久、南後守
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      東大阪
    • Related Report
      2011 Final Research Report 2009 Self-evaluation Report
  • [Presentation] 1.5Kで使える反射対物レンズの球面収差及び色収差の改善2008

    • Author(s)
      藤原正規、藤芳暁、松下道雄
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      東大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] ニ光子蛍光による低温の単一タンパク質分光2008

    • Author(s)
      藤芳暁、松下道雄
    • Organizer
      日本物理学会第63回年次大会
    • Place of Presentation
      東大阪
    • Related Report
      2011 Final Research Report 2009 Self-evaluation Report
  • [Presentation] Single-molecule spectroscopy of light-harvesting 2 complex at liquid-helium temperature2007

    • Author(s)
      H. Oikawa, S. Fujiyoshi, T. Dewa, M. Nango, and M. Matsushita
    • Organizer
      The 2nd international workshop on "Photosynthetic Antennae and Coherent Phenomena"
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure of light-harvesting 2 complex from Rps. acidophila in lipid bilayer of DMPC: A single-molecule study at 5 K2007

    • Author(s)
      D. Uchiyama, H. Oikawa, S. Fujiyoshi, T. Dewa, M. Nango, and M. Matsushita
    • Organizer
      The 2nd international workshop on "Photosynthetic Antennae and Coherent Phenomena"
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Spectroscopy of Single Proteins at Liquid Helium Temperature2007

    • Author(s)
      M. Matsushita and S. Fujiyoshi
    • Organizer
      The 2nd International Workshop on "Materials Science and Nano-Engineering"
    • Place of Presentation
      Awaji Island, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Spectroscopy of Single Proteins at Liquid Helium Temperature2007

    • Author(s)
      M. Matsushita
    • Organizer
      The 67th Okazaki International Conference on "Molecular science and chemical biology of biomolecular function"
    • Place of Presentation
      Okazaki, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 人工脂質膜中における光合成アンテナ複合体の会合体の分光2007

    • Author(s)
      内山大輔、古屋陽、藤芳暁、松下道雄、出羽毅久、南後守
    • Organizer
      日本物理学会第62回年次大会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 可視蛍光をプローブとした低温の単一タンパク質分光2007

    • Author(s)
      藤芳暁、藤原正規、松下道雄
    • Organizer
      第1回分子科学討論会
    • Place of Presentation
      仙台
    • Related Report
      2011 Final Research Report
  • [Presentation] 二波長の励起光を同時に利用できる低温の単一タンパク質分光装置の開発2007

    • Author(s)
      平野充遥、藤原正規、藤芳暁、松下道雄、伊関峰生、渡辺正勝
    • Organizer
      第1回分子科学討論会
    • Place of Presentation
      仙台
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.molphys.phys.titech.ac.jp

    • Related Report
      2011 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2018-03-28  

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