Structure and function relationship of enzymes studied by single-molecule spectroscopy at liquid-helium temperature
| Project Area | Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process |
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| Project/Area Number |
19056011
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MATSUSHITA Michio 東京工業大学, 大学院・理工学研究科, 准教授 (80260032)
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| Co-Investigator(Kenkyū-buntansha) |
藤芳 暁 東京工業大学, 大学院・理工学研究科, 助教 (70371705)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIYOSHI Satoru 東京工業大学, 理工学研究科, 助教 (70371705)
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥35,000,000 (Direct Cost: ¥35,000,000)
Fiscal Year 2011: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2010: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2009: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2008: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2007: ¥8,400,000 (Direct Cost: ¥8,400,000)
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| Keywords | 単一分子分光 / タンパク質 / 分光 / 低温 / 構造機能相関 / 一分子 / タンパク / 分子 / 一分子計測 |
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| Research Abstract |
Physical and chemical properties of enzyme can be studied using a homogeneous solution of a purified sample of enzyme. However, in living cells, individual protein molecules of enzyme are in different locations to experience different environments, so that they function differently. For understanding enzymatic reactions on the basis of molecular science, observation of individual molecules is essential. In this study we realized spectroscopy of visible-fluorescent single protein molecules.
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Report
(7 results)
Research Products
(49 results)
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[Journal Article] Single-protein study of photoresistance of pigment-protein complex in lipid bilayer2011
Author(s)
D. Uchiyama, H. Hoshino, K. Otomo, T. Kato, K. Onda, A. Watanabe, H. Oikawa, S. Fujiyoshi, M. Matsushita, M. Nango, N. Watanabe, A. Sumino, T. Dewa
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Journal Title
Chem. Phys. Lett.
Volume: 511
Pages: 135-135
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