| Project Area | Cell Proliferation Control |
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| Project/Area Number |
19057008
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | 公益財団法人がん研究会・がん研究所 (2011)
Japanese Foundation For Cancer Research (2007-2010) |
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Principal Investigator |
HIROTA Toru 公益財団法人がん研究会・がん研究所, 実験病理部, 部長 (50421368)
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| Co-Investigator(Renkei-kenkyūsha) |
高垣 謙太郎 公益財団法人がん研究会がん研究所, 実験病理部, 博士研究員 (70419951)
内田 和彦 公益財団法人がん研究会がん研究所, 実験病理部, 博士研究員 (40380555)
進藤 軌久 公益財団法人がん研究会がん研究所, 実験病理部, 博士研究員 (00512253)
熊田 和貴 公益財団法人がん研究会がん研究所, 実験病理部, 博士研究員 (10370149)
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥64,100,000 (Direct Cost: ¥64,100,000)
Fiscal Year 2011: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2010: ¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2009: ¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2008: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 2007: ¥18,900,000 (Direct Cost: ¥18,900,000)
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| Keywords | 細胞分裂 / 染色体 / 紡錘体 / 分裂期キナーゼ / 動原体 / Poloキナーゼ / Auroraキナーゼ / コンデンシン複合体 / コヒーシン複合体 / サイクリン依存性キナーゼ / コンデンシン / リン酸化 / Aurora B / セントロメア / 染色体分配 / 微小管 / 紡錘体チェックポイント / FRAP解析 |
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| Research Abstract |
Cell division is a basis of cell proliferation, and thus must be under rigorous control. The research aimed to elucidate how mitotic apparatus are regulated by mitotic kinases including Cdk1, Aurora and Polo kinases. To study this, we first established a series of cell lines that stably express fluorescence labeled proteins in chromosomes, kinetochores and microtubules. We then set up a condition to study protein dynamics using the fluorescence recovery after photobleaching assay. Using these cell lines and technique, we obtained the following results: 1) Aurora B promotes the release of HP1α from chromosome arms in mitosis, but a small subset of HP1α become a part of the chromosomal passenger-complex (CPC) in a phosphorylation-dependent manner. HP1α was found out to be an essential component of the CPC to prevent mis-segregation of chromosomes. 2) Cdk1 targets a subunit of condensin II complex, CAP-D3 to initiate chromosome condensation as cells enter mitosis. A Cdk1-mediated phosphorylation of CAP-D3 induces recruitment of Plk1 and further propagation of phosphorylation widely on the complex, which promotes condensin II to assemble chromosomes. These findings highlight the distinct properties of Aurora and Polo kinases in regulating mitotic apparatus.
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