Checkpoint analyses using anti -phosphoepitope-specific antibody
Project Area | Cell Proliferation Control |
Project/Area Number |
19057010
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Aichi Cancer Center Research Institute |
Principal Investigator |
INAGAKI Masaki (INAGAKI Msaski) 愛知県がんセンター(研究所), 発がん制御研究部, 部長 (30183007)
|
Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Makoto 名古屋市立大学, 医学(系)研究科(研究院), 教授 (40217774)
|
Project Period (FY) |
2007 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥150,500,000 (Direct Cost: ¥150,500,000)
Fiscal Year 2011: ¥32,500,000 (Direct Cost: ¥32,500,000)
Fiscal Year 2010: ¥32,500,000 (Direct Cost: ¥32,500,000)
Fiscal Year 2009: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2008: ¥28,500,000 (Direct Cost: ¥28,500,000)
Fiscal Year 2007: ¥28,500,000 (Direct Cost: ¥28,500,000)
|
Keywords | Chk1 / チェックポイント / リン酸化修飾 / 中心体 / G0/G1移行 / トリコプレイン / 中心体(中心小体) / Aurora-A / 自己リン酸化反応 / Cdc25A / 14-3-3 / ケラチン / 細胞周期 / 細胞分裂 / 細胞構築 / 抗リン酸化抗体 / Tip60 / リン酸化抗体 / 細胞がん化 / 細胞周期チェックポイント / サイクリン依存性キナーゼ / 細胞分裂期 / 蛋白質リン酸化 / 抗リン酸化ペプチド |
Research Abstract |
Chk1 is a protein kinase implicated in DNA maintenance checkpoint. Chk1 function is regulated through the phosphorylation of its regulatory domain. However, only ATR was long believed to functionally regulate Chk1. In this study, we have demonstrated that a variety of protein kinases phosphorylate and thereby regulate Chk1. In addition, we have also shown novel Chk1 function at the G0/G1 transition.
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Report
(7 results)
Research Products
(62 results)
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[Journal Article] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation2012
Author(s)
Li, P., Goto, H., Kasahara, K., Matsuyama, M., Wang, Z., Yatabe, Y., Kiyono, T., Inagaki, M.
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Journal Title
Mol. Biol. Cell
Volume: 23
Issue: 8
Pages: 1582-1592
DOI
Related Report
Peer Reviewed
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[Journal Article] Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein2011
Author(s)
Ibi, M., Zou, P., Inoko, A., Shiromizu, T., Matsuyama, M., Hayashi, Y., Enomoto, M., Mori, D., Hirotsune, S., Kiyono, T., Tsukita, S., Goto, H.. Inagaki, M.
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Journal Title
J. Cell Sci.
Volume: 124
Issue: 6
Pages: 857-864
DOI
Related Report
Peer Reviewed
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[Journal Article] Vimentin organization modulates the formation of lamellipodia2011
Author(s)
Helfand, B. T., Mendez, M. G., Murthy, S. N., Shumaker, D. K., Grin, B., Mahammad, S., Aebi, U., Wedig, T., Wu, Y. I., Hahn, K. M., Inagaki, M., Herrmann, H., Goldman, R. D.
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Journal Title
Mol. Biol. Cell
Volume: 22
Issue: 8
Pages: 1274-1289
DOI
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Peer Reviewed
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[Journal Article] Nuclear Chk1 prevents premature mitotic entry2011
Author(s)
Matsuyama, M., Goto, H., Kasahara, K., Kawakami, Y., Nakanishi, M., Kiyono, T., Goshima, N., Inagaki, M.
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Journal Title
J. Cell Sci.
Volume: 124
Issue: 13
Pages: 2113-2119
DOI
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Peer Reviewed
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[Journal Article] DNA lesions induced by replication stress trigger mitotic aberration and tetraploidy development2010
Author(s)
Ichijima, Y., Yoshioka, K., Yoshioka, Y., Shinohe, K., Fujimori, H., Unno, J., Takagi, M., Goto, H., Inagaki, M., Mizutani, S., Teraoka, H.
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Journal Title
PLoS ONE
Volume: 5
Issue: 1
Pages: e8821-e8821
DOI
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Peer Reviewed
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[Journal Article] Withaferin A targets intermediate filaments glial fibrillary acidic protein and vimentin in a model of retinal gliosis2010
Author(s)
Bargagna-Mohan, P., Paranthan, R. R., Hamza, A., Dimova, N., Trucchi, B., Srinivasan, C., Elliott, G. I., Zhan, C. G., Lau, D. L., Zhu, H., Kasahara, K., Inagaki, M., Cambi, F., Mohan, R.
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Journal Title
J. Biol. Chem.
Volume: 285
Issue: 10
Pages: 7657-7669
DOI
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Peer Reviewed
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[Journal Article] Association between vitamin D and calcium intake and breast cancer risk according to menopausal status and receptor status in Japan2010
Author(s)
Kawase, T., Matsuo, K., Suzuki, T., Hirose, K., Hosono, S., Watanabe, M., Inagaki, M., Iwata, H., Tanaka, H., Tajima, K.
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Journal Title
Cancer Sci.
Volume: 101
Issue: 5
Pages: 1234-1240
DOI
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Peer Reviewed
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[Journal Article] 14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage2010
Author(s)
Kasahara, K., Goto, H., Enomoto, M., Tomono, Y., Kiyono, T., Inagaki, M.
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Journal Title
EMBO J.
Volume: 29
Issue: 16
Pages: 2802-2812
DOI
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Peer Reviewed
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[Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during the G2/M transition2009
Author(s)
Enomoto, M., Goto, H., Tomono, Y., Kasahara, K., Tsujimura, K., Kiyono, T., Inagaki, M.
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Journal Title
J. Biol. Chem.
Volume: 284
Issue: 49
Pages: 34223-34230
DOI
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Peer Reviewed
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[Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during the G2/M transition.2009
Author(s)
Enomoto, M., Goto, H., Tomono, Y., Kasahara, K., Tsujimura, K., Kiyono, T., Inagaki, M.
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Journal Title
J.Biol.Chem. 284
Pages: 34223-34230
Related Report
Peer Reviewed
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[Journal Article] Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice2008
Author(s)
Li, Z. F., Wu, X., Jiang, Y., Liu, J., Wu, C., Inagaki, M., Izawa, I., Mizisin, A. P., Engvall, E., Shelton, G. D.
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Journal Title
J. Neurol. Sci.
Volume: 264
Issue: 1-2
Pages: 77-86
DOI
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Peer Reviewed
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[Journal Article] Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation2008
Author(s)
Toyo-oka, K., Mori, D., Yano, Y., Shiota, M., Iwao, H., Goto, H., Inagaki, M., Hiraiwa, N., Muramatsu, M., Wynshaw-Boris, A., Yoshiki, A., Hirotsune, S.
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Journal Title
J. Cell Biol.
Volume: 180
Issue: 6
Pages: 1133-1147
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[Journal Article] eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions2008
Author(s)
Lin, Y. M., Chen, Y. R., Lin, J. R., Wang, W. J., Inoko, A., Inagaki, M., Wu, Y. C., Chen, R. H.
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Journal Title
J. Cell Sci.
Volume: 121
Issue: 14
Pages: 2382-2393
DOI
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Peer Reviewed
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[Journal Article] The keratin-binding protein Albatross regulates polarization of epithelial cells2008
Author(s)
Sugimoto, M., Inoko, A., Shiromizu, T., Nakayama, M., Zou, P., Yonemura, S., Hayashi, Y., Izawa, I., Sasoh, M., Uji, Y., Kaibuchi, K., Kiyono, T., Inagaki, M.
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Journal Title
J. Cell Biol.
Volume: 183
Issue: 1
Pages: 19-28
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Peer Reviewed
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[Journal Article] Chk1 phosphorylation at Ser286 and Ser301 occurs with both stalled DNA replication and damage checkpoint stimulation2008
Author(s)
Ikegami, Y., Goto, H., Kiyono, T., Enomoto, M., Kasahara, K., Tomono, Y., Tozawa, K., Morita, A., Kohri, K., Inagaki, M.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 337
Issue: 4
Pages: 1227-1231
DOI
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Peer Reviewed
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[Journal Article] The keratin-binding protein Albatross regulates polarization of epithelial cells.2008
Author(s)
Sugimoto, M., Inoko, A., Shiromizu, T., Nakayama, M., Zou, P., Yonemura, S., Hayashi, Y., Izawa, I., Sasoh, M., Uji, Y., Kaibuchi, K., Kiyono, T., Inagaki, M.
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Journal Title
J.CellBiol. 183
Pages: 19-28
Related Report
Peer Reviewed
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[Journal Article] Inhibition of Aurora-B function increases formation of multinucleated cells in p53 gene deficient cells and enhances anti-tumor effect of temozolomide in human glioma cells2007
Author(s)
Tsuno, T., Natsume, A., Katsumata, S., Mizuno, M., Fujita, M., Osawa, H., Nakahara, N., Wakabayashi, T., Satoh, Y., Inagaki, M., Yoshida, J.
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Journal Title
J. Neurooncol.
Volume: 83
Issue: 3
Pages: 249-258
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Peer Reviewed
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[Journal Article] 14-3-3γ mediates Cdc25A proteolysis to induce S and G2 arrest after DNA damage.
Author(s)
Kasahara, K., Goto, H., Enomoto, M., Tomono, Y., Kiyono, T., Inagaki, M.
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Related Report
Peer Reviewed
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