Reconstitution of prions and protein folding

• Project Area: Protein community: organization and maintenance of protein functions
• Project/Area Number: 19058002
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology (2009-2011); The University of Tokyo (2007-2008)
• Principal Investigator: TAGUCHI Hideki 東京工業大学, 大学院・生命理工学研究科, 教授 (40272710)
• Co-Investigator(Kenkyū-buntansha): KOIKE Ayumi 神奈川工科大学, 応用バイオ科学部, 教授 (20454176)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥94,500,000 (Direct Cost: ¥94,500,000); Fiscal Year 2011: ¥18,900,000 (Direct Cost: ¥18,900,000); Fiscal Year 2010: ¥18,900,000 (Direct Cost: ¥18,900,000); Fiscal Year 2009: ¥18,900,000 (Direct Cost: ¥18,900,000); Fiscal Year 2008: ¥18,900,000 (Direct Cost: ¥18,900,000); Fiscal Year 2007: ¥18,900,000 (Direct Cost: ¥18,900,000)
• Keywords: タンパク質 / フォールディング / シャペロン / 酵母プリオン / アミロイド / プリオン / シャペロニン / 凝集体 / GroEL / 無細胞蛋白質合成系

Research Abstract

Protein must fold into unique tertiary structure to achieve its function. However, the folding often competes with intermolecular aggregation, which usually spoils the protein function. Protein aggregates include prions and amyloids, both of which cause fatal neurodegenerative diseases. We investigated following topics; 1) comprehensive aggregation analysis of more than 3,000 Escherichia coli proteins by using a reconstituted chaperone-free translation system, ii) global analysis of chaperone effects, iii) identification of in vivo chaperonin substrates, and iv) in vivo structure and dynamics of yeast prions.

Research Products

• [Journal Article] Biomolecular Robotics for Chemomechanically Driven Guest Delivery Fueled by Intracellular ATP (2013)
  • Author(s): Biswas, S., Kinbara, K., Niwa, T., Taguchi, H., Ishii, N., Watanabe, S., Miyata, K., Kataoka, K., Aida, T.
  • Journal Title: Nature Chemistry Volume: (published online)
  • Peer Reviewed
• [Journal Article] Nano-scale alignment of proteins on a flexible DNA back-bone (2012)
  • Author(s): Nojima, T., Konno, H., Kodera, N., Seio, K., Taguchi, H. and Yoshida, M
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Flexibility of GroES mobile loop is required for efficient chaperonin function (2012)
  • Author(s): Nojima, T., Ikegami, T., Taguchi, H. and Yoshida, M.
  • Journal Title: J. Mol. Biol Volume: 422 Pages: 291-299
  • Peer Reviewed
• [Journal Article] Global Analysis of Chaperone Effects Using a Reconstituted Cell-Free Translation System (2012)
  • Author(s): Niwa, T., Kanamori T., Ueda, T., Taguchi, H.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A Volume: 109 Pages: 8937-8942
  • Peer Reviewed
• [Journal Article] Mechanism of methionine synthase overexpression in chaperonin-depleted Escherichia coli (2012)
  • Author(s): Fujiwara K, Taguchi, H
  • Journal Title: Microbiology Volume: 158 Pages: 917-924
  • Peer Reviewed
• [Journal Article] Nano-scale alignment of proteins on a nexible DNA back-bone (2012)
  • Author(s): Nojima, T.*, Konno, H., Kodera, N., Seio, K, Taguchi, H., Yoshida, M.
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: 52534-52534
  • DOI: 10.1371/journal.pone.0052534
  • Peer Reviewed
• [Journal Article] Flexibility of GroES mobile loop is required for efficient chaperonin function (2012)
  • Author(s): Nojima, T., Ikegami, T., Taguchi, H., Yoshida, M.*
  • Journal Title: J. Mol. Biol. Volume: 422 Issue: 2 Pages: 291-299
  • DOI: 10.1016/j.jmb.2012.05.026
  • Peer Reviewed
• [Journal Article] Global Analysis of Chaperone Effects Using a Reconstituted Cell-Free Translation System (2012)
  • Author(s): Niwa, T., Kanamori T, Ueda, T.*, Taguchi, H.*
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 109 Issue: 23 Pages: 8937-8942
  • DOI: 10.1073/pnas.1201380109
  • Peer Reviewed
• [Journal Article] Difference in the distribution pattern of substrate enzymes in the metabolic network of Escherichia coli, according to chaperonin requirement (2011)
  • Author(s): Takemoto K, Niwa T, Taguchi, H
  • Journal Title: BMC Syst Biol Volume: 5 Pages: 98-98
  • Peer Reviewed
• [Journal Article] Amphiphilic Polysaccharide Nanogels as Artificial Chaperones in Cell-Free Protein Synthesis (2011)
  • Author(s): Sasaki, Y, Asayama, W., Niwa, T., Sawada, S., Ueda, T., Taguchi, H., Akiyoshi, K.
  • Journal Title: Macromol. Biosci Volume: 11 Pages: 814-820
  • Peer Reviewed
• [Journal Article] Yeast prion protein New1 can break Sup35 amyloid fibrils into fragments in an ATP-dependent manner (2011)
  • Author(s): Inoue, Y., Kawai-Noma, S., Koike-Takeshita, A., Taguchi, H. and Yoshida, M.
  • Journal Title: Genes to Cells Volume: 16 Pages: 545-556
  • Peer Reviewed
• [Journal Article] Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells (2011)
  • Author(s): Tsuji, T., Kawai-Noma, S., Pack, C-G., Terajima, H., Yajima, J., Nishizaka, T., Kinjo, M. & Taguchi, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 405 Pages: 638-643
  • Peer Reviewed
• [Journal Article] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome (2011)
  • Author(s): Zhou, Z-P., Shimizu, Y., Tadakuma, H., Taguchi, H., Ito, K. and Ueda, T
  • Journal Title: J. Biochem Volume: 149 Pages: 609-618
  • NAID: 10030585460
  • Peer Reviewed
• [Journal Article] Difference in the distribution pattern of substrate enzymes in the metabolic network of Escherichia coli, according to chaperonin requirement. (2011)
  • Author(s): Takemoto K*, Niwa T, Taguchi H.
  • Journal Title: BMC Syst Biol. Volume: 5 Issue: 1 Pages: 98-98
  • DOI: 10.1186/1752-0509-5-98
  • Peer Reviewed
• [Journal Article] Amphiphilic Polysaccharide Nanogels as Artificial Chaperones in Cell-Free Protein Synthesis (2011)
  • Author(s): Sasaki, Y, Asayama, W., Niwa, T., Sawada, S., Ueda, T., Taguchi, H., Akiyoshi, K.*
  • Journal Title: Macromol. Biosci. Volume: 11 Issue: 6 Pages: 814-820
  • DOI: 10.1002/mabi.201000457
  • Peer Reviewed
• [Journal Article] Yeast prion protein Newl can break Sup35 amyloid fibrils into fragments in an ATP-dependent manner. (2011)
  • Author(s): Inoue, Y., Kawai-Noma, S., Koike-Takeshita, A., Taguchi, H., Yoshida, M.*
  • Journal Title: Genes to Cells Volume: 16 Issue: 5 Pages: 545-556
  • DOI: 10.1111/j.1365-2443.2011.01510.x
  • Peer Reviewed
• [Journal Article] Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells (2011)
  • Author(s): Tsuji T, Kawai-Noma S, Pack CG, Terajima H, Yajima J, Nishizaka T, Kinjo M, Taguchi H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 405(4) Issue: 4 Pages: 638-643
  • DOI: 10.1016/j.bbrc.2011.01.083
  • Peer Reviewed
• [Journal Article] Single molecule imaging of the trans-translation entry process via anch oring of the tagged ribosome. (2011)
  • Author(s): Zhou, Z-P., Shimizu, Y., Tadakuma, H.*, Taguchi, H., Ito, K., Ueda, T.
  • Journal Title: J. Biochem. Volume: 149 Issue: 5 Pages: 609-618
  • DOI: 10.1093/jb/mvr010
  • NAID: 10030585460
  • Peer Reviewed
• [Journal Article] A. In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells (2010)
  • Author(s): Kawai-Noma, S., Pack, C-G., Kojidani, T., Asakawa, H., Hiraoka, Y., Kinjo, M., Haraguchi, T., Taguchi, H., and Hirata
  • Journal Title: J. Cell Biol Volume: 190 Pages: 223-231
  • Peer Reviewed
• [Journal Article] A systematic survey of in vivo obligate chaperonin-dependent substrates (2010)
  • Author(s): Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T. and Taguchi, H.
  • Journal Title: EMBO J Volume: 29 Pages: 1552-1564
  • Peer Reviewed
• [Journal Article] Diffuse oligomer-based transmission of yeast prions. (Review) (2010)
  • Author(s): Taguchi, H. and Kawai-Noma, S
  • Journal Title: FEBS J Volume: 277 Pages: 1359-1368
  • Peer Reviewed
• [Journal Article] In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells. (2010)
  • Author(s): Kawai-Noma, S., Pack, C-G, Kojidani, T., Asakawa, H., Hiraoka, Y., Kinjo, M., Haraguchi, T., Taguchi, H., Hirata, A.
  • Journal Title: J.Cell Biol. Volume: 190 Pages: 223-231
  • Peer Reviewed
• [Journal Article] A systematic survey of in vivo obligate chaperonin-dependent substrates. (2010)
  • Author(s): Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T., Taguchi, H.
  • Journal Title: EMBO J. Volume: 29 Pages: 1552-1564
  • Peer Reviewed
• [Journal Article] Diffuse oligomer-based transmission of yeast prions. (2010)
  • Author(s): Taguchi, H., Kawai-Noma, S.
  • Journal Title: FEBS J. Volume: 277 Pages: 1359-1368
  • Peer Reviewed
• [Journal Article] A systematic survey of in vivo obligate chaperonin-dependent substrates. (2010)
  • Author(s): Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T., Taguchi, H.
  • Journal Title: EMBO J. (in press)
  • Peer Reviewed
• [Journal Article] A systematic survey of in vivo obligate chaperonin-dependent substrates. (2010)
  • Author(s): Fujiwara, K., Ishihama, Y., Nakahigashi, K., Soga, T., Taguchi, H.
  • Journal Title: EMBO Journal Volume: 29 Pages: 1552-1564
  • Peer Reviewed
• [Journal Article] In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells. (2010)
  • Author(s): Kawai-Noma, S., Pack, C-G, Kojidani, T., Asakawa, H., Hiraoka, Y., Kinjo, M., Haraguchi, T., Taguchi, H., Hirata, A.
  • Journal Title: Journal of Cell Biology Volume: 190 Pages: 223-231
  • Peer Reviewed
• [Journal Article] Diffuse oligomer-based transmission of yeast prions. (2010)
  • Author(s): Taguchi, H., Kawai-Noma, S.
  • Journal Title: FEBS Journal Volume: 277 Pages: 1359-1368
  • Peer Reviewed
• [Journal Article] Diffuse oligomer-based transmission of yeast prions. (Review) (2010)
  • Author(s): Taguchi, H., Kawai-Noma, S.
  • Journal Title: FEBS J. ((印刷中)掲載確定)
  • Peer Reviewed
• [Journal Article] D-loop of actin differently regulates the motor function of myosins II And V (2009)
  • Author(s): Kubota, H., Mikhailenko, S. V., Okabe, H., Taguchi, H., and Ishiwata, S.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 35251-35258
  • Peer Reviewed
• [Journal Article] Single mother-daughter pair analysis to analyze the diffusion properties of yeast prion Sup35 in guanidine-HCl treated [PSI+] cells (2009)
  • Author(s): Kawai-Noma, S., Pack, C-G., Tsuji, T., Kinjo, M., Taguchi, H.
  • Journal Title: Genes to Cells Volume: 14 Pages: 1045-1054
  • Peer Reviewed
• [Journal Article] A tubular biocontainer: Metal ion-induced 1D assembly of a molecularly engineered chaperonin (2009)
  • Author(s): Biswas, S., Kinbara, K., Oya, N., Ishii, N., Taguchi, H., Aida, T.
  • Journal Title: J. Am. Chem. Soc Volume: 131 Pages: 7556-7557
  • Peer Reviewed
• [Journal Article] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins (2009)
  • Author(s): Niwa, T., Ying, B.-W., Saito, K., Jin, W. Z., Takada, S., Ueda, T. Taguchi, H.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 106 Pages: 4201-4206
  • Peer Reviewed
• [Journal Article] Cryo-EM structure of the native GroEL-GroES complex from Thermus thermophilus encapsulating substrate inside the cavity (2009)
  • Author(s): Kanno, R., Koike-Takeshita, A., Yokoyama, K., Taguchi, H., Mitsuoka, K.
  • Journal Title: Structure Volume: 17 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins. (2009)
  • Author(s): Niwa, T., Ying, B.-W., Saito, K., Jin, W.Z., Takada, S., Ueda, T. Taguchi, H.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 106 Pages: 4201-4206
  • Peer Reviewed
• [Journal Article] Cryo-EM structure of the native GroEL-GroES complex from Thermus thermop hilus encapsulating substrate inside the cavity. (2009)
  • Author(s): Kanno, R., Koike-Takeshita, A., Yokoyama, K., Taguchi, H., Mitsuoka, K
  • Journal Title: Structure 17 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins (2009)
  • Author(s): Niwa, T., Ying, B.-W., Saito, K., Jin, W.Z., Takada, S., Ueda, T.Taguchi, H.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 106 Pages: 4201-4206
  • Peer Reviewed
• [Journal Article] A tubular biocontainer : Metal ion-induced 1D assembly of a molecularly engineered chaperonin (2009)
  • Author(s): Biswas, S., Kinbara, K., Oya, N., Ishii, N., Taguchi, H., Aida, T.
  • Journal Title: J.Am.Chem.Soc. 131 Pages: 7556-7557
  • Peer Reviewed
• [Journal Article] Single mother-daughter pair analysis to analyze the diffusion properties of yeast prion Sup35 in guanidine-HCl treated [PSI+] cells (2009)
  • Author(s): Kawai-Noma, S., Pack, C-G., Tsuji, T., Kinjo, M., Taguchi, H.
  • Journal Title: Genes to Cells 14 Pages: 1045-1054
  • Peer Reviewed
• [Journal Article] D-loop of actin differently regulates the motor function of myosins II And V (2009)
  • Author(s): Kubota, H., Mikhailenko, S.V., Okabe, H., Taguchi, H., Ishiwata, S.
  • Journal Title: J.Biol.Chem. 284 Pages: 35251-35258
  • Peer Reviewed
• [Journal Article] シャペロニン・基質複合体の分子構造 (2009)
  • Author(s): 光岡薫、小池あゆみ、田口英樹
  • Journal Title: 生物物理学会誌 49 Pages: 296-297
  • NAID: 10025976011
  • Peer Reviewed
• [Journal Article] 大腸菌全蛋白質の凝集体解析 (2009)
  • Author(s): 丹羽達也、上田卓也、田口英樹
  • Journal Title: 蛋白質核酸酵素(共立出版) 54 Pages: 1870-1875
• [Journal Article] Kinetic analysis of conformational changes of GroEL based on the fluorescence of tyrosine 506 (2008)
  • Author(s): Hosono, K., Ueno, T., Taguchi, H., Motojima, F., Zako, T., Yoshida, M., Funatsu, T.
  • Journal Title: Protein J Volume: 27 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Revisiting the GroEL-GroES reaction cycle via the symmetrical intermediate implied by novel aspects of the GroEL (D398A) mutant (2008)
  • Author(s): Koike-Takeshita, A., Yoshida, M., Taguchi, H.
  • Journal Title: J. Biol. Chem Volume: 283 Pages: 23774-23781
  • Peer Reviewed
• [Journal Article] Single molecule imaging of full protein synthesis by immobilized ribosomes (2008)
  • Author(s): Uemura, S., Iizuka, R., Ueno, T., Shimizu, Y., Taguchi, H., Ueda, T., Puglisi, J., Funatsu, T.
  • Journal Title: Nucleic Acids Research Volume: 36
  • Peer Reviewed
• [Journal Article] Comparison of refolding activities between nanogel artificial chaperone and GroEL systems (2008)
  • Author(s): Asayama, W., Sawada, S., Taguchi, H., Akiyoshi, K.
  • Journal Title: Int. J. Biol. Macromol Volume: 42 Pages: 241-246
  • Peer Reviewed
• [Journal Article] Revisiting the GroEL-GroES reaction cycle via the symmetrical intermediate implied by novel aspects of the GroEL (D398A) mutant. (2008)
  • Author(s): Koike-Takeshita, A., Yoshida, M., Taguchi, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 23774-23781
  • Peer Reviewed
• [Journal Article] Single molecule imaging of full protein synthesis by immobilized ribosomes. (2008)
  • Author(s): Uemura, S., Iizuka, R., Ueno, T., Shimizu, Y., Taguchi, H., Ueda, T., Puglisi, J., Funatsu, T.
  • Journal Title: Nucleic Acids Research 36
  • Peer Reviewed
• [Journal Article] Revisiting the GroEL-GroES reaction cycle via the symmetrical intermediate implied by novel aspects of the GroEL (D398A) mutant. (2008)
  • Author(s): Koike-Takeshita, A., Yoshida, M., Taguchi, H.
  • Journal Title: □J.Biol.Chem. 283 Pages: 23774-23781
  • Peer Reviewed
• [Journal Article] Kinetic analysis of conformational changes of GroEL based on the fluorescence of tyrosine 506 (2008)
  • Author(s): Hosono, K., Ueno, T., Taguchi, H., Motojima, F., Zako, T., Yoshida, M., Funatsu, T.
  • Journal Title: Protein J. 27 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Comparison of refolding activities between nanogel artificial chaperone and CroEL systems (2008)
  • Author(s): Asayama, W., Sawada, S., Taguchi, H., Akiyoshi, K.
  • Journal Title: Int. J. Biol. Macromol. 印刷中
  • Peer Reviewed
• [Journal Article] Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE. (2007)
  • Author(s): Fujiwara, K., Taguchi, H.
  • Journal Title: J. Bacteriol. 189 Pages: 5860-5866
  • Peer Reviewed
• [Journal Article] Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE. (2007)
  • Author(s): Fujiwara, K. Taguchi, H.
  • Journal Title: J. Bacteriol. 189 Pages: 5860-5866
  • Peer Reviewed
• [Presentation] In vivo structure and dynamics of yeast prion protein (2013)
  • Author(s): Hideki Taguchi
  • Organizer: SPIE 2013: Nano-Bio Sensing, Imaging & Spectroscopy
  • Place of Presentation: Jeju-do, Korea
• [Presentation] In vivo structure and dynamics of yeast prion protein (2013)
  • Author(s): Hideki Taguchi
  • Organizer: SPIE 2013 : Nano-Bio Sensing, Imaging & Spectros copy.
  • Place of Presentation: Jeju-do, (Korea)(招待講演)
• [Presentation] Global analyses of protein aggregation and chaperone effects (2012)
  • Author(s): Hideki Taguchi
  • Organizer: SFB 3rd International symposium "Molecular Machines in Protein Folding and Protein Transport"
  • Place of Presentation: Munich, Germany
• [Presentation] Global analyses of protein aggregation and chaperone effects (2012)
  • Author(s): Hideki Taguchi,
  • Organizer: SFB 3rd International symposium "Molecular Machines in Protein Folding and Protein Transport"
  • Place of Presentation: Munich, (Germany)(招待講演)
• [Presentation] 大腸菌のシャペロニンGroELの細胞内での役割 (2012)
  • Author(s): 田口英樹
  • Organizer: 日本蛋白質科学会年会ワークショップ「ここまでわかったシャペロニン:作動機構と細胞内での役割」
  • Place of Presentation: 名古屋国際会議場(愛知県)(招待講演)
• [Presentation] Global analysis of chaperone action using a reconstituted cell-free translation system (2011)
  • Author(s): Hideki Taguchi
  • Organizer: EMBO Conference "The Biology of Molecular Chaperones"
  • Place of Presentation: Grundlsee, Austria
• [Presentation] Global analysis of chaperone action using a reconstituted cell-free translation system (2011)
  • Author(s): 田口英樹
  • Organizer: EMBO Conference "The Biology of Molecular Chaperones"
  • Place of Presentation: Grundlsee, (Austria)(招待講演)
• [Presentation] Evolution of protein folding in the cell (2010)
  • Author(s): 田口英樹
  • Organizer: 第33回分子生物学会年会、第83回生化学会大会合同年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] A systematic survey of in vivo obligate chaperonin GroE-dependent substrates (2010)
  • Author(s): Hideki Taguchi
  • Organizer: 3rd International Symposium on Protein Society
  • Place of Presentation: ホテル日航奈良、奈良
• [Presentation] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins (2009)
  • Author(s): 田口英樹
  • Organizer: EMBO Conference "The Biology of Molecular Chaperones"
  • Place of Presentation: ドブロブニク(クロアチア)
  • Year and Date: 2009-05-24
• [Presentation] Mechanism of yeast prion propagation revealed by direct observation of prion protein dynamics (2009)
  • Author(s): 田口英樹
  • Organizer: International Conference "Protein Folding and Neurodegenerative Diseases"
  • Place of Presentation: 京都
  • Year and Date: 2009-04-06
• [Presentation] A comprehensive survey of in vivo obligate GroEL/ES substrates (2009)
  • Author(s): Hideki Taguchi
  • Organizer: 4th International Congress on Stress Responses in Biology and Medicine, Symposium "From chaperones to Translocators"
  • Place of Presentation: 札幌
• [Presentation] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins (2009)
  • Author(s): Hideki Taguchi
  • Organizer: EMBO Conference "The Biology of Molecular Chaperones"
  • Place of Presentation: Dubrovnik, Croatia
• [Presentation] Mechanism of yeast prion propagation revealed by direct observation of prion protein dynamics (2009)
  • Author(s): Hideki Taguchi
  • Organizer: International Conference "Protein Folding and Neurodegenerative Diseases"
  • Place of Presentation: 平安会館、京都
• [Presentation] Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins. (2009)
  • Author(s): Hideki Taguchi
  • Organizer: EMBO Conference "The Biology of Molecular Chaperones"
  • Place of Presentation: Dubrovnik, Croatia
• [Presentation] Direct observation of yeast prion dynamics in single-living cells (2008)
  • Author(s): 田口英樹
  • Organizer: 2nd Pacific Rim International Conference on Protein Science
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2008-06-24
• [Presentation] Direct observation of yeast prion Sup35 dynamics in single-living cells (2008)
  • Author(s): 田口英樹
  • Organizer: Cold Spring Harbor Laboratory meeting on Molecular Chaperones and Stress Responses
  • Place of Presentation: Cold Spring Harbor(米国)
  • Year and Date: 2008-05-02
• [Presentation] Direct observation of yeast prion dynamics in single-living cells (2008)
  • Author(s): Hideki Taguchi
  • Organizer: 2nd Pacific Rim International Conference on Protein Science
  • Place of Presentation: Cairns convention centre, Cairns, Australia
• [Presentation] Direct observation of yeast prion Sup35 dynamics in single-living cells (2008)
  • Author(s): Hideki Taguchi
  • Organizer: Cold Spring Harbor Laboratory meeting on Molecular Chaperones and Stress Responses
  • Place of Presentation: Cold Spring Harbor Laboratory, New York, USA
• [Presentation] シャペロニンGroELの反応モデルの改訂と2ストロークモデルの可能性 (2007)
  • Author(s): 小池あゆみ、吉田 賢右、田口 英樹
  • Organizer: 日本分子生物学会日本生化学会合同年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Role of Chaperonin GroEL on the folding of newly translated polypeptides. (2007)
  • Author(s): 田口 英樹
  • Organizer: 7th Kias-Soogsil conference on Protein Structure and Folding
  • Place of Presentation: ソウル
  • Year and Date: 2007-10-05
• [Presentation] シャペロニンGroELの細胞内基質蛋白質の解析 (2007)
  • Author(s): 田口 英樹
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-06-29
• [Presentation] 細胞内での酵母プリオンの増殖および伝搬機構 (2007)
  • Author(s): 田口 英樹
  • Organizer: 日本蛋白質科学会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-26
• [Book] 実験医学30(「史上最長の実験は続くよ」) (2012)
  • Author(s): 田口英樹
  • Publisher: 羊土社
• [Book] バイオサイエンスとインダストリー70(「綱羅約な解析から見えてきたタンバク質凝集特性とシャペロンの役割」) (2012)
  • Author(s): 丹羽達也、田口英樹
  • Total Pages: 6
  • Publisher: JBA
• [Book] 実験医学29,(「自然免疫誘導にプソオン的な自己増殖が関与」) (2011)
  • Author(s): 田口英樹
  • Publisher: 羊土社
• [Book] 実験医学29(「βシートからなるアミロイド形成の発端はコイルドコイル構造?」) (2011)
  • Author(s): 田口英樹30, 田口英樹
  • Publisher: 羊土社
• [Book] キーワード:蛋白質の一生 (2008)
  • Author(s): 編集・遠藤斗志也、小椋光、永田和宏、森 和俊、田口 英樹、吉田 賢右
  • Publisher: 共立出版
• [Book] タンパク質の一生:集中マスター (2007)
  • Author(s): 遠藤斗志也, 森和俊, 田口英樹
  • Total Pages: 147
  • Publisher: 羊土社
• [Book] タンパク質の一生 集中マスター 細胞における成熟・輸送・品質管理 (2007)
  • Author(s): 田口 英樹(編集)
  • Total Pages: 146
  • Publisher: 羊土社
• [Book] ナノバイオ計測の実際 (2007)
  • Author(s): 上野 太郎、田口 英樹(分担)
  • Publisher: 講談社サイエンティフィック
• [Remarks] 大腸菌蛋白質可溶性データベース:eSol
  • URL: http://www.tanpaku.org/tp-esol
• [Remarks] 田口研究室ウェブサイト
  • URL: http://www.taguchi.bio.titech.ac.jp
• [Remarks] 田口英樹の補足情報
  • URL: http://taguchi-hideki.blogspot.jp
• [Remarks]
  • URL: http://www.taguchi.bio.titech.ac.jp
• [Remarks]
  • URL: http://molbio.chem.t.u-tokyo.ac.jp/taguchi/
• [Remarks] eSOL:大腸菌全タンパク質の凝集率のデータベース
  • URL: http://tp-esol.genes.nig.ac.jp/