Mechanisms of remodeling and quality control of proteins by AAA chaperones

• Project Area: Protein community: organization and maintenance of protein functions
• Project/Area Number: 19058012
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University
• Principal Investigator: OGURA Teru 熊本大学, 発生医学研究所, 教授 (00158825)
• Co-Investigator(Kenkyū-buntansha): YAMANAKA Kunitoshi 熊本大学, 発生医学研究所, 准教授 (90212290) ; ESAKI Masatoshi 熊本大学, 発生医学研究所, 助教 (70437911)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥73,000,000 (Direct Cost: ¥73,000,000); Fiscal Year 2011: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2010: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2009: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2008: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2007: ¥14,600,000 (Direct Cost: ¥14,600,000)
• Keywords: AAA タンパク質 / 分子シャペロン / ATPase / 線虫 / 酵母 / 神経変性疾患 / AAAタンパク質 / Aurora Bキナーゼ / 高速原子間力顕微鏡 / TDP-43 / 微小管 / ATP加水分解 / ミトコンドリア / 品質管理機構 / spastin / ClpX / Bcs1p / 線虫の性決定 / 性決定機構 / トランスロケーション / タンパク質凝集体

Research Abstract

Systematic analysis uncovered the mechanism common to several AAA chaperones. The importance of cooperativity of the ATPase activity of D2 in p97/VCP/CDC-48/Cdc48p has been demonstrated. High-speed atomic force microscopy revealed repeated rotational movements of the p97 ring in the presence of ATP. Using yeasts and nematodes, cellular and developmental roles of AAA chaperones such as p97, fidgetin, and Bcs1p were elucidated. These results shed light on the molecular mechanisms of human diseases related to these AAA chaperones.

Research Products

• [Journal Article] Caenorhabditis elegans fidgetin homolog FIGL-1, a nuclear-localized AAA ATPase, binds to SUMO. (2012)
  • Author(s): Onitake, A., Yamanaka, K., Esaki, M., and Ogura, T.
  • Journal Title: J. Struct. Biol. Volume: (in press) Issue: 2 Pages: 143-151
  • DOI: 10.1016/j.jsb.2012.04.022
  • Peer Reviewed
• [Journal Article] Cdc48p/p97-mediated regulation of mitochondrial morphology is Vms1p independent. (2012)
  • Author(s): Esaki, M., and Ogura, T.
  • Journal Title: J. Struct. Biol. Volume: (in press) Issue: 2 Pages: 112-120
  • DOI: 10.1016/j.jsb.2012.04.017
  • Peer Reviewed
• [Journal Article] The C-terminal -helix of SPAS-1, a Caenorhabditis elegans spastin homologue, is crucial for microtubule severing. (2012)
  • Author(s): Onitake, A., Matsushita-Ishiodori, Y., Johjima, A., Esaki, M., Ogura, T., and Yamanaka, K.
  • Journal Title: J. Struct. Biol. Volume: (in press) Issue: 2 Pages: 138-142
  • DOI: 10.1016/j.jsb.2012.04.010
  • Peer Reviewed
• [Journal Article] Recent advances in p97/VCP/Cdc48 cellular functions. (2012)
  • Author(s): Yamanaka, K., Sasagawa, Y., and Ogura, T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1823 Issue: 1 Pages: 130-137
  • DOI: 10.1016/j.bbamcr.2011.07.001
  • NAID: 120004141045
  • Peer Reviewed
• [Journal Article] The C-terminal α-helix of SPAS-1, a C.elegans spastin homologue, is crucial for microtubule severing (2012)
  • Author(s): Onitak, A.
  • Journal Title: J.Struct.Biol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cdc48p/p97-mediated regulation of mitochondrial morphology is Vms1p-independent (2012)
  • Author(s): Esaki, M.
  • Journal Title: J.Struct.Biol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Positive cooperativity of the p97 AAA ATPase is critical for essential functions. (2011)
  • Author(s): Nishikori, S., Esaki, M., Yamanaka, K., Sugimoto, S., and Ogura, T.
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 18 Pages: 15815-15820
  • DOI: 10.1074/jbc.m110.201400
  • Peer Reviewed
• [Journal Article] C. elegans UBX cofactors for CDC-48/p97 control spermatogenesis. (2010)
  • Author(s): Sasagawa, Y., Yamanaka, K. Saito-Sasagawa, Y., and Ogura, T.
  • Journal Title: Genes Cells 15 Volume: 15 Issue: 12 Pages: 1201-1215
  • DOI: 10.1111/j.1365-2443.2010.01454.x
  • Peer Reviewed
• [Journal Article] FtsH cleavage of non-native conformations of proteins. (2010)
  • Author(s): Ayuso-Tejedor, S., Nishikori, S., Okuno, T., Ogura, T., and Sancho, J.
  • Journal Title: J. Struct. Biol. Volume: 171 Issue: 2 Pages: 117-124
  • DOI: 10.1016/j.jsb.2010.05.001
  • Peer Reviewed
• [Journal Article] High-speed atomic force microscopy techniques for observing dynamic biomolecular processes. (2010)
  • Author(s): Yamamoto, D., Uchihashi, T., Kodera, N., Yamashita, H., Nishikori, S., Ogura, T., Shibata, M., and Ando, T.
  • Journal Title: Methods Enzymol. Volume: 475 Pages: 541-564
  • DOI: 10.1016/s0076-6879(10)75020-5
  • Peer Reviewed
• [Journal Article] AAA+ chaperone ClpX regulates dynamics of prokaryotic cytoskeletal protein FtsZ. (2010)
  • Author(s): Sugimoto, S., Yamanaka, K., Nishikori, S., Miyagi, A., Ando, T., and Ogura, T.
  • Journal Title: J. Biol. Chem. Volume: 285 Issue: 9 Pages: 6648-6657
  • DOI: 10.1074/jbc.m109.080739
  • Peer Reviewed
• [Journal Article] ATP-bound form of the D1 AAA domain inhibits an essential function of Cdc48p/p97. (2010)
  • Author(s): Esaki, M., and Ogura, T.
  • Journal Title: Biochem. Cell Biol. Volume: 88 Issue: 1 Pages: 109-117
  • DOI: 10.1139/o09-116
  • Peer Reviewed
• [Journal Article] C.elegans UBX cofactors for CDC-48/p97 control spermatogenesis. (2010)
  • Author(s): Sasagawa, Y.
  • Journal Title: Genes Cells Volume: 15 Pages: 1201-1215
  • Peer Reviewed
• [Journal Article] FtsH cleavage of non-native conformations of proteins. (2010)
  • Author(s): Ayuso-Tejedor, S.
  • Journal Title: J.Struct.Biol. Volume: 171 Pages: 117-124
  • Peer Reviewed
• [Journal Article] AAA+ chaperone ClpX regulates dynamics of prokaryotic cytoskeletal protein FtsZ. (2010)
  • Author(s): Sugimoto, S., Yamanaka, K., Nishikori, S., Miyagi, A.,o, T., Ogura, T.
  • Journal Title: J. Biol. Chem. 285 Pages: 6648-6657
  • Peer Reviewed
• [Journal Article] High-speed atomic force microscopy techniques for observing dynamic biomolecular processes. (2010)
  • Author(s): Yamamoto, D.
  • Journal Title: Methods Enzymol. (In press)
• [Journal Article] AAA^+ chaperone ClpX regulates dynamics of prokaryotic cytoskeletal protein FtsZ. (2010)
  • Author(s): Sugimoto, S.
  • Journal Title: J.Biol.Chem. 285 Pages: 6648-6657
  • Peer Reviewed
• [Journal Article] ATP-bound form of the D1 AAA domain inhibits an essential function of Cdc48p/p97. (2010)
  • Author(s): Esaki, M.
  • Journal Title: Biochem.Cell Biol. 88 Pages: 109-117
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans p97 controls germline-specific sex determination by controlling TRA-1 level in a CUL-2-dependent manner. (2009)
  • Author(s): Sasagawa, Y., Otani, M., Higashitani, N., Higashitani, A., Sato, K., Ogura, T., and Yamanaka, K.
  • Journal Title: J. Cell Sci. Volume: 122 Issue: 20 Pages: 3663-3672
  • DOI: 10.1242/jcs.052415
  • Peer Reviewed
• [Journal Article] Conserved aromatic and basic amino acid residues in the pore region of C. elegans spastin play critical roles in microtubule-severing. (2009)
  • Author(s): Matsushita-Ishiodori, Y., Yamanaka, K., Hashimoto, H., Esaki, M., and Ogura, T.
  • Journal Title: Genes Cells Volume: 14 Issue: 8 Pages: 925-940
  • DOI: 10.1111/j.1365-2443.2009.01320.x
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans p97 controls germline-specific sex determination by controlling TRA-1 level in a CUL-2-dependent manner. (2009)
  • Author(s): Sasagawa, Y., Otani, M., Higashitani, N., Higashitani, A., Sato, K., Ogura, T., Yamanaka, K.
  • Journal Title: J. Cell Sci. 122 Pages: 3663-3672
  • Peer Reviewed
• [Journal Article] onserved aromatic and basic amino acid residues in the pore region of C. elegans spastin play critical roles in microtubule-severing. (2009)
  • Author(s): Matsushita-Ishiodori, Y., Yamanaka, K., Hashimoto, H., Esaki, M., Ogura, T.
  • Journal Title: Genes Cells 14 Pages: 925-940
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans p97 controls germline-specific sex determination by controlling TRA-1 level in a CUL-2-dependent manner. (2009)
  • Author(s): Sasagawa, Y.
  • Journal Title: J.Cell Sci. 122 Pages: 3663-3672
  • Peer Reviewed
• [Journal Article] Conserved aromatic and basic amino acid residues in the pore region of C.elegans spastin play critical roles in microtubule-severing. (2009)
  • Author(s): Matsushita-Ishiodori, Y.
  • Journal Title: Genes Cells 14 Pages: 925-940
  • Peer Reviewed
• [Journal Article] p97 homologs from C. elegans, CDC-48.1 and CDC-48.2, suppress the aggregate formation of huntingtin exon1 containing expanded polyQ repeat. (2008)
  • Author(s): Nishikori, S., Yamanaka, K., Sakurai, T., Esaki, M., and Ogura, T.
  • Journal Title: Genes Cells Volume: 13 Issue: 8 Pages: 827-838
  • DOI: 10.1111/j.1365-2443.2008.01214.x
  • Peer Reviewed
• [Journal Article] Involvement of HMG-12 and CAR-1 in the cdc-48.1 expression of Caenorhabditis elegans. (2008)
  • Author(s): Yamauchi, S., Higashitani, N., Otani, M., Higashitani, A., Ogura, T., and Yamanaka, K.
  • Journal Title: Dev. Biol. Volume: 318 Issue: 2 Pages: 348-359
  • DOI: 10.1016/j.ydbio.2008.02.057
  • Peer Reviewed
• [Journal Article] From the common molecular basis of the AAA protein to various energy-dependent and -independent activities of AAA proteins. (2008)
  • Author(s): Ogura, T., Matsushita-Ishiodori, Y., Johjima, A., Nishizono, M., Nishikori, S., Esaki, M., and Yamanaka, K.
  • Journal Title: Biochem. Soc. Trans. Volume: 36 Issue: 1 Pages: 68-71
  • DOI: 10.1042/bst0360068
  • Peer Reviewed
• [Journal Article] T. p97 homologs from C. elegans, CDC-48. 1 and CDC-48. 2, suppress the aggregate formation of huntingtin exon1 containing expanded polyQ repeat. (2008)
  • Author(s): Nishikori, S., Yamanaka, K., Sakurai, T., Esaki, M., Ogura, T.
  • Journal Title: Genes Cells 13 Pages: 827-838
  • Peer Reviewed
• [Journal Article] p97 homologs from C. elegans, CDC-48.1 and CDC-48.2, suppress the aggregate formation of huntingtin exonl containing expanded polyQ repeat (2008)
  • Author(s): Nishikori, S.
  • Journal Title: Genes Cells 13 Pages: 827-838
  • Peer Reviewed
• [Journal Article] Involvement of HMG-12 and CAR-1 in the cdc-48.1 expression of Caenorhabditis elegans (2008)
  • Author(s): Yamauchi, S.
  • Journal Title: Dev. Biol 318 Pages: 827-838
  • Peer Reviewed
• [Journal Article] From the common molecular basis of the AAA protein to various energy-dependent and-independent activities of AAA proteins (2008)
  • Author(s): Ogura, T.
  • Journal Title: Biochem. Soc. Trans 36 Pages: 68-71
• [Journal Article] From common molecular basis of the AAA protein to various energy-dependent and independent activities of AAA proteins. (2008)
  • Author(s): Ogura, T.
  • Journal Title: Biochem. Soc. Trans. 36 Pages: 68-71
  • Peer Reviewed
• [Journal Article] Involvement of HMG-12 and CAR-1 in the cdc-48.1 expression of Caenorhabditis elegans. (2008)
  • Author(s): Yamauchi, S.
  • Journal Title: Dev. Biol. (in press)
  • Peer Reviewed
• [Journal Article] ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in C. Elegans. (2007)
  • Author(s): Sasagawa, Y., Yamanaka, K., and Ogura, T.
  • Journal Title: Genes Cells Volume: 12 Issue: 9 Pages: 1063-1073
  • DOI: 10.1111/j.1365-2443.2007.01108.x
  • Peer Reviewed
• [Journal Article] The C. elegans homologue of the spastic paraplegia protein, spastin, disassembles microtubules. (2007)
  • Author(s): Matsushita-Ishiodori, Y., Yamanaka, K., and Ogura, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 359 Issue: 1 Pages: 157-162
  • DOI: 10.1016/j.bbrc.2007.05.086
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans p97/CDC-48 is crucial for progression of meiosis I. (2007)
  • Author(s): Sasagawa, Y., Yamanaka, K., Nishikori, S., and Ogura, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 358 Issue: 3 Pages: 920-924
  • DOI: 10.1016/j.bbrc.2007.05.022
  • Peer Reviewed
• [Journal Article] Differential expression pattern of UBX family genes in Caenorhabditis elegans. (2007)
  • Author(s): Yamauchi, S., Sasagawa, Y., Ogura, T., and Yamanaka, K.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 358 Issue: 2 Pages: 545-552
  • DOI: 10.1016/j.bbrc.2007.04.163
  • Peer Reviewed
• [Journal Article] C. elegans RBX-2-CUL-5- and RBX-1-CUL-2- based complexes are redundant for oogenesis and activation of the MAP kinase MPK-1. (2007)
  • Author(s): Sasagawa, Y., Sato, S., Ogura, T., and Higashitani, A.
  • Journal Title: FEBS Lett. Volume: 581 Issue: 1 Pages: 145-150
  • DOI: 10.1016/j.febslet.2006.12.009
  • Peer Reviewed
• [Journal Article] ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in C. elegans. (2007)
  • Author(s): Sasagawa, Y., Yamanaka, K., Ogura, T.
  • Journal Title: Genes Cells 12 Pages: 1063-1073
  • Peer Reviewed
• [Journal Article] Differential expression pattern of UBX family genes in Caenorhabditis elegans. (2007)
  • Author(s): Yamauchi, S.
  • Journal Title: Biochem. Biophys. Res. Commun. 358 Pages: 545-552
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans p97/CDC-48 is crucial for progression of meiosis I. (2007)
  • Author(s): Sasagawa, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 358 Pages: 920-924
  • Peer Reviewed
• [Journal Article] The C. elegans homologue of the spastic paraplegia protein, spastin, disassembles microtubules. (2007)
  • Author(s): Matsushita-Ishiodori, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 359 Pages: 157-162
  • Peer Reviewed
• [Journal Article] ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in C. elegans. (2007)
  • Author(s): Sasagawa, Y,
  • Journal Title: Genes Cells 12 Pages: 1063-1073
  • Peer Reviewed
• [Journal Article] Positive cooperativity of the p97 AAA ATPase is critical for essential functions.
  • Author(s): Nishikori, S.
  • Journal Title: J.Biol.Chem. Volume: (In press)
  • Peer Reviewed
• [Presentation] Multiple aspects of multifunctional p97. (2011)
  • Author(s): Ogura, T., Noi, K., Arita-Morioka, K., Esaki, M., Murayama, Y., and Yamanaka, K.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館, Kumamoto, Japan.
• [Presentation] Characterization of a fidgetin homologue, a member of AAA proteins, in C.elegans (2011)
  • Author(s): Murayama, Y.
  • Organizer: 18th International C.elegans Meeting
  • Place of Presentation: UCLA, USA
• [Presentation] CDC-48/p97 is required for meiotic chromosome segregation in C.elegans (2011)
  • Author(s): Sasagawa, Y.
  • Organizer: 18th International C.elegans Meeting
  • Place of Presentation: UCLA, USA
• [Presentation] Mutations in p97/Cdc48p affect mitochondrial morphology (2011)
  • Author(s): Esaki, M.
  • Organizer: 18th International C.elegans Meeting
  • Place of Presentation: UCLA, USA
• [Presentation] DNA polymerase V in E.coli (2011)
  • Author(s): Karata, K.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Observation and characterization of ATP-dependent dynamic movements of the p97 AAA ATPase using high-speed atomic force microscopy (2011)
  • Author(s): Noi, K.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Molecular mechanism of microtubule-severing by the AAA chaperone katanin (2011)
  • Author(s): Johjima, A.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Characterization of a fidgetin homologue, a member of AAA proteins, in C.elegans (2011)
  • Author(s): Onitake, A.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Role of Bcs1p, a mitochondrial inner membrane AAA protein, in the respiratory chain complex III assembly (2011)
  • Author(s): Sawamura, R.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Multiple aspects of multifunctional p97 (2011)
  • Author(s): Ogura, T
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)(招待講演)
• [Presentation] A ubiquitin-selective AAA chaperone Cdc48p/p97 regulates mitochondrial morphology (2011)
  • Author(s): Esaki, M.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] Observation and characterization of dynamic ATP-dependent movements of p97 AAA ATPase using high-speed atomic force microscopy (2011)
  • Author(s): Noi, K.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] Regulation of cellular functions of sumoylated proteins by fidgetin (2011)
  • Author(s): Onitake, A.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] Analysis of CDC-48/p97 on the meiotic chromosome segregation in C.elegans (2011)
  • Author(s): Sasagawa, Y.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] Role of Bcs1p in the respiratory chain complex III assembly (2011)
  • Author(s): Sawamura, R.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] Antagonistic control of CDC-48 by C-terminal adaptors UFD-2 and UFD-3 in C.elegans (2011)
  • Author(s): Murayama, Y.
  • Organizer: 9th International Conference on AAA Protein
  • Place of Presentation: 熊本市国際交流会館(熊本)
• [Presentation] A ubiquitin-selective AAA chaperone Cdc48p/p97 regulates mitochondrial morphology (2011)
  • Author(s): Esaki, M.
  • Organizer: 2011年度特定領域「タンパク質の社会」全体班会議
  • Place of Presentation: 別府湾ロイヤルホテル(大分)
• [Presentation] 高速AFMを用いたVCP/p97のナノダイナミクス解析 (2011)
  • Author(s): 野井健太郎
  • Organizer: 2011年度特定領域「タンパク質の社会」全体班会議
  • Place of Presentation: 別府湾ロイヤルホテル(大分)
• [Presentation] AAタンパク質kataninによる微小管切断機構 (2011)
  • Author(s): 城島愛
  • Organizer: 2011年度特定領域「タンパク質の社会」全体班会議
  • Place of Presentation: 別府湾ロイヤルホテル(大分)
• [Presentation] AAAタンパク質fidgetin/FIGL-1はSUMOと相互作用する (2011)
  • Author(s): 鬼武彰宣
  • Organizer: 2011年度特定領域「タンパク質の社会」全体班会議
  • Place of Presentation: 別府湾ロイヤルホテル(大分)
• [Presentation] ミトコンドリア呼吸鎖複合体III会合におけるAAAタンパク質Bcs1pの機能解析 (2011)
  • Author(s): 澤村理英
  • Organizer: 2011年度特定領域「タンパク質の社会」全体班会議
  • Place of Presentation: 別府湾ロイヤルホテル(大分)
• [Presentation] 高速AFMを用いた分子シャペロンp97のダイナミクス解析 (2011)
  • Author(s): 野井健太郎
  • Organizer: CREST「生命現象の解明と応用に資する新しい計測・分析基盤技術」研究領域シンポジウム
  • Place of Presentation: 東京ガーデンパレス(東京)
• [Presentation] A cytosolic AAA chaperone Cdc48p/p97 regulates mitochondrial morphology (2011)
  • Author(s): Esaki, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] AAAタンパク質fidgetinの線虫ホモログFIGL-1によるSUMO化タンパク質の活性制御機構の解明 (2011)
  • Author(s): 鬼武彰宣
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] 呼吸鎖複合体IIIにおけるAAA分子シャペロンBcs1pの分子機構の解析 (2011)
  • Author(s): 澤村理英
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] 線虫CDC-48のC末端アダプターUFD-2,UFD-3の細胞機能解析 (2011)
  • Author(s): 村山佑樹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] バクテリア細胞内タンパク質のアミロイド様凝集体形成 (2011)
  • Author(s): 杉本真也
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Mutations in p97/Cdc48p Affect Mitochondrial Morphology. (2011)
  • Author(s): Esaki, M.
  • Organizer: Keystone Symposia's Meeting on : AAA+ and Related ATP-Driven Protein Machines : Structure, Function and Mechanism
  • Place of Presentation: Granlibakken Resort, Tahoe City, USA
• [Presentation] AAA型シャペロンVCP/p97と神経変性疾患IBMPFD (2010)
  • Author(s): 小椋光
  • Organizer: 新潟脳神経研究会第290回例会
  • Place of Presentation: 新潟大学脳研究所新潟
  • Year and Date: 2010-06-04
• [Presentation] 線虫を用いたp97/VCP/CDC48の機能解析 (2010)
  • Author(s): 山中邦俊
  • Organizer: 新潟脳神経研究会第290回例会
  • Place of Presentation: 新潟大学脳研究所新潟
  • Year and Date: 2010-06-04
• [Presentation] Involvement of C. elegans p97-cofactor complexes in sex determination. (2010)
  • Author(s): Yamanaka, K., Sasagawa, Y., Murayama, Y., and Ogura, T.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良, Nara, Japan
• [Presentation] C. elegans CDC-48/p97 controls germline-specific sex determination. (2010)
  • Author(s): Yamanaka, K., Sasagawa, Y., and Ogura, T.
  • Organizer: BIT Life Sciences' 3rd Annual Protein and Peptide Conference (PepCon-2010)
  • Place of Presentation: Beijing, China
• [Presentation] Roles of p97, a multifunctional AAA chaperone, in C. elegans development. (2010)
  • Author(s): Ogura. T.
  • Organizer: Academia Sinica-Kumamoto University Joint Conference on Organogenesis, B1C Auditorium, Institute of Biomedical Sciences, Academia Sinica
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Involvement of C.elegans p97-cofactor complexes in sex determination. (2010)
  • Author(s): Yamanaka, K.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良Nara, Japan
• [Presentation] Cooperative ATPase activity of p97/CDC48p controls mitochondrial morphology. (2010)
  • Author(s): Esaki, M.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良Nara, Japan
• [Presentation] Characterization of dynamic movements of the p97 AAA ATPase using high-speed atomic force microscopy. (2010)
  • Author(s): Noi, K.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良Nara, Japan
• [Presentation] Nucleotide-dependent formation of amyloid-like aggregates of Escherichia coli cytoplasmic proteins. (2010)
  • Author(s): Sugimoto, S.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良 Nara, Japan
• [Presentation] C.elegans CDC-48/p97 controls germline-specific sex determination. (2010)
  • Author(s): Yamanaka, K.
  • Organizer: BIT Life Sciences' 3rd Annual Protein and Peptide Conference (PepCon-2010)
  • Place of Presentation: International Convention Center, Beijing, China
• [Presentation] Roles of p97, a multifunctional AAA chaperone, in C.elegans development. (2010)
  • Author(s): Ogura, T.
  • Organizer: Academia Sinica-Kumamoto University Joint Conference on Organogenesis, B1C Auditorium, Institute of Biomedical Sciences, Academia Sinica
  • Place of Presentation: 中央研究院, Taipei, Taiwan
• [Presentation] Molecular mechanisms of homologs of spastin and p97/VCP related to human genetic disorders. (2009)
  • Author(s): Ogura, T., Matsushita-Ishiodori, Y., Yamanaka, K., Nishikori, S., and Esaki, M.
  • Organizer: 8th International Conference on AAA Proteins
  • Place of Presentation: Toronto, Canada
• [Presentation] Roles of p97/VCP, a multifunctional AAA chaperone, in protein oligomerization and aggregation in neurodegenerative diseases. (2009)
  • Author(s): Ogura, T., Nishikori, S., Yamanaka, K., and Esaki, M.
  • Organizer: BIT Life Sciences' 2_<nd> Annual Protein and Peptide Conference (PepCon-2009)
  • Place of Presentation: Seoul, South Korea
• [Presentation] Single-molecule analysis of dynamics of bacterial cytoskeletal protein FtsZ by high-speed AFM. (2009)
  • Author(s): Sugimoto, S.
  • Organizer: JST/CREST International Symposium Watching Biomolecules in Action ; Single Molecule Biology Symposium 2nd Kanazawa Bio-AFM Workshop
  • Place of Presentation: Osaka, Japan千里ライフサイエニスセニター
• [Presentation] Cellular functions of p97, a multifunctional AAA chaprone. (2009)
  • Author(s): Ogura, T.
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: Sapporo, Japanガトーキングダム札幌ホテル
• [Presentation] Positive cooperativity of the p97 AAA ATPase is critical for the function. (2009)
  • Author(s): Nishikori, S.
  • Organizer: 23rd annual symposium of the protein society "PROTEINS IN MOTION"
  • Place of Presentation: Boston, USA
• [Presentation] Positive cooperativity of the p97 AAA ATPase is critical for cell viability. (2009)
  • Author(s): Esaki, M.
  • Organizer: 8th International Conference on AAA Proteins
  • Place of Presentation: Toronto, Canada
• [Presentation] Remodeling of cell division protein FtsZ by AAA^+ chaperone CIpX from E.coli. (2009)
  • Author(s): Sugimoto, S.
  • Organizer: 8th International Conference on AAA Proteins
  • Place of Presentation: Toronto, Canada
• [Presentation] C.elegans p97/CDC-48UFD-1-NPL-4 controls germline specific sex determination by regulating CUL-2-mediated TRA-1 degradation. (2009)
  • Author(s): Yamanaka, K.
  • Organizer: 8th International Conference on AAA Proteins
  • Place of Presentation: Toronto, Canada
• [Presentation] Roles of p97/VCP, a multifunctional AAA chaperone, in protein oligomerization and aggregation in neurodegenerative diseases. (2009)
  • Author(s): Ogura, T.
  • Organizer: BIT Life Sciences' 2nd Annual Protein and Peptide Conference (PepCon-2009)
  • Place of Presentation: Seoul, South Korea
• [Presentation] NMRによるAAAタンパク質spastinの線虫ホモログSPAS-1の微小管結合様式の解析 (2009)
  • Author(s): 松下(石躍)由佳
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] SUMO化タンパク質と特異的に相互作用する線虫fidgetinホモログFIGL-1の細胞機能 (2009)
  • Author(s): 鬼武彰宣
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Positive Cooperativity of the p97 AAA ATPase is Critical for Cell Viability (2009)
  • Author(s): 江崎雅俊
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] p97/VCPとタンパク質凝集体 (2009)
  • Author(s): 江崎雅俊
  • Organizer: 第9回日本蛋白質科学年会、ワークショップAAAファミリー蛋白質と疾患
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
• [Presentation] 大腸菌AAA^+シャペロンClpXによる細胞分裂関連タンパク質FtsZのリモデリング (2009)
  • Author(s): 杉本真也
  • Organizer: 第9回日本蛋白質科学年会、ワークショップAAAファミリー蛋白質と疾患
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
• [Presentation] 微小管切断AAAタンパク質spastinの線虫ホモログSPAS-1の微小管結合ドメインの構造解析 (2009)
  • Author(s): 松下(石躍)由佳
  • Organizer: 第9回日本蛋白質科学年会、ワークショップAAAファミリー蛋白質と疾患
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本県)
• [Presentation] 線虫p97ホモログCDC-48.1の協同的ATP加水分解機構 (2008)
  • Author(s): 錦織伸吾
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
• [Presentation] UBXファミリータンパク質は, 精子形成過程, 卵母細胞形成過程, 初期胚発生における細胞極性を制御する (2008)
  • Author(s): 笹川洋平
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
• [Presentation] p97/CDC-48.1による線虫生殖細胞の性決定の制御 (2008)
  • Author(s): 山中邦俊
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] AAAタンパク質spastinの線虫ホモログSPAS-1による微小管切断機構の解析 (2008)
  • Author(s): 松下(石躍)由佳
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Positive Cooperativity of the p97 AAA ATPase is critical for cell viability (2008)
  • Author(s): Esaki, M.
  • Organizer: 48th ASCB Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Analysis of microtubule severing by the C. elegans homologue SPAS-1, a member of AAA proteins (2008)
  • Author(s): Matsushita-Ishiodori, Y.
  • Organizer: 48th ASCB Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] From common molecular basis of the AAA ATPase to various energy-dependent and independent activities of AAA proteins. (2007)
  • Author(s): Ogura, T.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Royal Agricultural College, Cirencester, UK.
• [Presentation] From common molecular basis of the AAA ATPase to various energy-dependent and independent activities of AAA proteins. (2007)
  • Author(s): Ogura, T.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] Functional analysis of p97NCP in C. elegans. (2007)
  • Author(s): Yamanaka, K.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] Regions for substrate interaction in C. elegans p97 homologs. (2007)
  • Author(s): Esaki, M.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] p97NCP homologs, CDC-48.1 and CDC-48.2, from C. elegans suppress the aggregation of huntingtin exon1. (2007)
  • Author(s): Nishikori, S.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] Characterization of the C. elegans homologue of spastin. (2007)
  • Author(s): Matsushita-Ishiodori, Y.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] Mode of action of katanin on microtubules. (2007)
  • Author(s): Johjima, A.
  • Organizer: The 7th International conference on AAA proteins
  • Place of Presentation: Cirencester, UK
• [Presentation] p97-UBXN complexes are essential for embryogenesis and gametogenesis in Caenorhabditis elegans. (2007)
  • Author(s): Sasagawa, Y.
  • Organizer: 16th biennial International C. elegans conference
  • Place of Presentation: UCLA, USA.
• [Presentation] C. elegans p97, HRD-1 and BiP are required for ERAD and developmental growth. (2007)
  • Author(s): Yamanaka, K.
  • Organizer: 16th biennial International C. elegans conference
  • Place of Presentation: UCLA, USA.
• [Presentation] Cdc48p/p97のATPaseドメインの特異な性質 (2007)
  • Author(s): 江崎 雅俊
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] AAAタンパク質spastinの線虫ホモログSPAS-1は微小管ダイナミクスに関与する (2007)
  • Author(s): 松下 由佳
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Kataninの微小管への作用 (2007)
  • Author(s): 城島 愛
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 線虫p97ホモログCDC-48.1の協同的ATP加水分解機構 (2007)
  • Author(s): 錦織 伸吾
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
• [Book] FtsH protese-mediated regulation of various cellular functions. In General and regulatory proteolysis(D.A.Dougan (ed.)) (2012)
  • Author(s): Okuno, T.
  • Publisher: Springer SBM(In press)
• [Book] FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition(N.Rawlings and G.Salvesen (ed.)) (2012)
  • Author(s): Ogura, T.
  • Publisher: Elsevier Ltd.(In press)
• [Book] High-speed atomic force microscopy techniques for observing dynamic biomolecular processes. Methods Enzymol (2010)
  • Author(s): Yamamoto, D.
  • Publisher: Elsevier Ltd.
• [Book] FtsH protease, a eubacterial membrane-bound AAA protease. In ATP-dependent proteases (Research Signport, ed. E. Kutejova) pp.87-114 (2008)
  • Author(s): Okuno, T., and Ogura, T.
• [Book] ATP-dependent proteases (2008)
  • Author(s): Okuno, T.
  • Total Pages: 27
  • Publisher: Research Signport
• [Book] ATP-dependent proteases (Research Signport, ed. E. Kutejova) (2008)
  • Author(s): Okuno, T.
  • Publisher: FtsH protease, a eubacterial membrane-bound AAA protease.(In press)
• [Book] 医学のための細胞生物学(永田和宏, 塩田浩平(編))(南山堂) (2008)
  • Author(s): 笹川綿子
  • Publisher: モデル生物の選択における利点と限界(印刷中)
• [Book] タンパク質の一生集中マスター(バイオ研究マスターシリーズ(遠藤斗志也, 森 和俊, 田口英樹(編))(羊土社) (2007)
  • Author(s): 小椋 光
  • Total Pages: 139
  • Publisher: シャペロン界の偶像破壊者AAA型シャペロン
• [Book] FtsH protease-mediated regulation of various cellular functions., In General and regulatory proteolysis. D. A. Dougan (ed.)
  • Author(s): Okuno, T., and Ogura, T.
  • Publisher: Springer SBM (in press)
• [Book] FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition, N. Rawlings and G. Salvesen(ed)
  • Author(s): Ogura, T., Okuno, T., Suno, R., and Akiyama, Y.
  • Publisher: Elsevier Ltd., (in press)
• [Book] FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition, N.Rawlings and G.Salvesen (ed)
  • Author(s): Ogura, T.
  • Publisher: Elsevier Ltd.(in press)
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/fukusei/index.html
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/AAA/aaainfo.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/fukusei/index.html
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/AAA/aaainfo.html
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/fukusei/index.html
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/fukusei/index.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/
• [Remarks]
  • URL: http://mukb.medic.kumamoto-u.ac.jp/fukusei/index.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/molecular_cell_biology/