Organelle targeting and integration system for membrane proteins
Project Area | Protein community: organization and maintenance of protein functions |
Project/Area Number |
19058013
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | University of Hyogo |
Principal Investigator |
SAKAGUCHI Masao 兵庫県立大学, 大学院・生命理学研究科, 教授 (30205736)
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Co-Investigator(Kenkyū-buntansha) |
木田 祐一郎 兵庫県立大学, 大学院・生命理学研究科, 助教 (10423899)
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Project Period (FY) |
2007 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥73,000,000 (Direct Cost: ¥73,000,000)
Fiscal Year 2011: ¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2010: ¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2009: ¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2008: ¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2007: ¥14,600,000 (Direct Cost: ¥14,600,000)
|
Keywords | 膜タンパク質 / シグナル配列 / トポロジー / 生体膜 / オルガネラ / 小胞体 / 膜蛋白質 / フォールディング / 生合成 |
Research Abstract |
In this project, we found the follows. The signal sequence, which triggers translocation of polypeptide chain, provides the motive force for polypeptide chain translocation. Positive charges of polypeptide chain cause stop-translocation in the membrane. A certain signal sequences suppress the targeting of hydrophobic segment to endoplasmic reticulum and mediate correct sorting to the cell organelle other than endoplasmic reticulum.
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Report
(7 results)
Research Products
(129 results)
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[Journal Article] Multiple organelle-targeting signals in the N-terminal portion of peroxisomal membrane protein PMP702010
Author(s)
Iwashita, S., Tsuchida, M., Tsukuda, M., Yamashita, Y., Emi, Y., Kida, Y. Komori, M., Kashiwayama, Y., Imanaka, T. and Sakaguchi, M.
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Journal Title
J. Biochem.
Volume: 147(4)
Pages: 581-590
NAID
Related Report
Peer Reviewed
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[Journal Article] Multiple organelle-targeting signals in the N-terminal portion of peroxisomal membrane protein PMP702010
Author(s)
Iwashita, S., Tsuchida, M., Tsukuda, M., Yamashita, Y., Emi, Y., Kida, Y.Komori, M., Kashiwayama, Y., Imanaka, T., Sakaguchi, M.
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Journal Title
J.Biochem.
Volume: 147(4)
Pages: 581-590
NAID
Related Report
Peer Reviewed
-
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[Journal Article] Multiple organelle-targeting signals in the N-terminal portion of peroxisomal membrane protein PMP702010
Author(s)
Iwashita, S., Tsuchida, M., Tsukuda, M., Yamashita, Y., Emi, Y., Kida,Y. Komori, M., Kashiwayama, Y., Imanaka, T., Sakaguchi, M.
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Journal Title
J.Biochem. 147(4)
Pages: 581-590
NAID
Related Report
Peer Reviewed
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[Journal Article] 70-kDa peroxisomal membrane protein related protein (P70R/ABCD4) localizes to endoplasmic reticulum not peroxisomes, and NH2-terminal hydrophobic property determines the subcellular localization of ABC subfamily D proteins2009
Author(s)
Kashiwayama, Y., Seki, M., Yasui, A., Murasaki, Y., Morita, M., Yamashita, Y., Sakaguchi, M., Tanaka, Y., Imanaka, T.
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Journal Title
Exp. Cell Res.
Volume: 315
Pages: 190-205
Related Report
Peer Reviewed
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[Journal Article] 70-kDa peroxisomal membrane protein related protein (P70R/ABCD4) localizes to endoplasmic reticulum not peroxisomes, and NH2-terminalhydrophobic property determines the subcellular localization of ABC subfamily D proteins2009
Author(s)
Kashiwayama, Y., Seki, M., Yasui, A., Murasaki, Y., Morita, M., Yamashita, Y., Sakaguchi, M., Tanaka, Y., Imanaka, T.
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Journal Title
Exp.Cell Res. 315
Pages: 190-205
Related Report
Peer Reviewed
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[Journal Article] 70-kDa peroxisomal membrane protein related protein (P7OR/ABCD4) localizes to endoplasmic reticulum not peroxisomes, and NH_<2->terminal hydrophobic property determines the subcellular localization of ABC subfamily D proteins2009
Author(s)
Kashiwayama, Y., Seki, M., Yasui, A., Murasaki, Y., Morita, M., Yamashita, Y., Sakaguchi, M., Tanaka, Y., Imanaka, T
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Journal Title
Exp. Cell Res 315
Pages: 190-205
Related Report
Peer Reviewed
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[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K+-channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K., Kodama, I., Sakaguchi, M., and Uozumi. N.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 104
Pages: 8263-8268
Related Report
Peer Reviewed
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[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K+-channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K., Kodama, I., Sakaguchi, M., Uozumi. N.
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Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 8263-8268
Related Report
Peer Reviewed
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[Journal Article] Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K-+-^channel voltage-sensor domain2007
Author(s)
Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.-K, Kodama, I., Sakaguchi, M., and Uozumi, N
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Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 8263-8268
Related Report
Peer Reviewed
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[Presentation] Positive charges in translocating polypeptide chain regulate the movement through translocon2011
Author(s)
Fujita, H., Kida, Y., Yamagishi, M., Sakaguchi, M.
Organizer
EMBO conference series "Protein transport systems : structures, mechanisms, and medical aspects"
Place of Presentation
Santa Margherita di Pula, Italy
Related Report
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[Presentation] ER-targeting Suppression by Short N-terminal Motif of Peroxisomal ABC Transporter, PMP702011
Author(s)
Sakaguchi, M., Iwashita, S., Yamashita, Y., Sakaue, H., Kida, Y.
Organizer
第63回日本細胞生物学会大会
Place of Presentation
北海道大学(北海道)
Related Report
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