| Budget Amount *help |
¥83,000,000 (Direct Cost: ¥83,000,000)
Fiscal Year 2011: ¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 2010: ¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 2009: ¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 2008: ¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 2007: ¥16,600,000 (Direct Cost: ¥16,600,000)
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| Research Abstract |
Foxp3^+ regulatory T (Treg) cells play a key role in establishing and maintaining natural self-tolerance. We have established a novel method for high-throughput analysis of the T cell receptor (TCR) repertoire using the next generation sequencing technology. By applying this method, we have analyzed the TCR repertoires of Foxp3^+ and Foxp3^- T cells and found that they are largely distinct. While this dissimilarity was established within the thymus, the repertoire of Foxp3^+ T cells was reshaped substantially in the periphery. In addition, we have also found that the normal T cell repertoire harbored a population of Foxp3- helper T cells with previous history of Foxp3 expression and that their TCR repertoire was distinct from Foxp3^+ Treg cells. Our results suggest that TCR specificity plays a central role in the lineage commitment of Treg cells.
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