| Budget Amount *help |
¥109,800,000 (Direct Cost: ¥109,800,000)
Fiscal Year 2012: ¥18,300,000 (Direct Cost: ¥18,300,000)
Fiscal Year 2011: ¥18,300,000 (Direct Cost: ¥18,300,000)
Fiscal Year 2010: ¥18,300,000 (Direct Cost: ¥18,300,000)
Fiscal Year 2009: ¥18,300,000 (Direct Cost: ¥18,300,000)
Fiscal Year 2008: ¥18,300,000 (Direct Cost: ¥18,300,000)
Fiscal Year 2007: ¥18,300,000 (Direct Cost: ¥18,300,000)
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| Research Abstract |
We have identified various factors involved in vascular tissue formation. In this study, we attempted to understand the function of these factors and their interrelationship at a molecular level, in view of following three aspects: (1) Regulation of vascular meristem formation by intercellular CLE peptides, (2) Regulation of vascular tissue formation by transcription factors, (3) Regulation of vascular pattern formation by spatial control of subcellular components. As a result, (1) we found intercellular and intracellular signaling pathways of CLE peptides regulating vascular meristem formation. In addition, we also indicated that TDIF functions in not only vascular but also axillary meristem formation. (2) Machinery of transcriptional regulation by VND6 via the TERE sequence was uncovered. Moreover, it was shown that bHLH transcription factors act at the initiation of vascular differentiation probably by inducing auxin transport. (3) We identified VAN3-ARF-GAP and MIDD1 as crucial factors governing cell polarity. In conclusion, we succeeded in demonstrating that vascular stem cell fates are determined by a specific transcription factor(s) regulated by a CLE peptide-dependent intracellular signaling pathway composed of a receptor kinase and a cellular kinase.
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