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Identification of ligand-receptor pairs in plants

Planned Research

Project AreaPlant regulatory systems that control developmental interactions between meristems and lateral organs
Project/Area Number 19060010
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute for Basic Biology (2010)
Nagoya University (2007-2009)

Principal Investigator

MATSUBAYASHI Yoshikatsu  基礎生物学研究所, 分子遺伝学研究部門, 教授 (00313974)

Co-Investigator(Kenkyū-buntansha) 篠原 秀文  基礎生物学研究所, 分子遺伝学研究部門, 助教 (40547022)
Project Period (FY) 2007 – 2012
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥53,600,000 (Direct Cost: ¥53,600,000)
Fiscal Year 2010: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2009: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2008: ¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2007: ¥13,400,000 (Direct Cost: ¥13,400,000)
Keywords分泌型ペプチド / 受容体キナーゼ / 細胞間情報伝達 / 翻訳後修飾 / リガンド-受容体相互作用 / ペプチドホルモン / 受容体 / シロイヌナズナ
Research Abstract

ペプチドホルモンの翻訳後修飾のひとつであるチロシン硫酸化酵素(TPST)遺伝子を破壊したシロイヌナズナ植物体(tpst-1)では,根端の幹細胞が維持されず,根が極端に短くなる.この表現型は,既知のチロシン硫酸化ペプチドホルモンの培地への添加では回復できなかったことから,新しい硫酸化ペプチドホルモンの存在を強く示唆していた.我々は,既知のチロシン硫酸化ペプチドの硫酸化モチーフ配列を参考にしたin silico遺伝子スクリーニングとnano LC-MSを用いた成熟型ペプチド構造解析,および合成ペプチドとチロシン硫酸化酵素破壊株tpst-1を用いた回復実験によって,根端メリステム幹細胞ニッチの維持およびメリステム活性の制御に関与する新規硫酸化ペプチド群(root meristem growth factors : RGFs)を見出した.9種類あるRGFペプチドファミリーの少なくとも4種類はQCとコルメラ幹細胞および最内層のコルメラ細胞を中心に発現しており,分泌されたペプチドは幹細胞領域からメリステム領域にかけて組織内を拡散していた.RGF遺伝子群の単独破壊株では表現型が表われないが,3重破壊株rgf1rgf2rgf3は,根端メリステム領域がかなり縮小する表現型を示した.またRGFは,根の幹細胞維持および細胞分裂活性の制御に中心的な役割を担う転写因子,PLETHORA (PLT)の発現を主として転写後レベルで制御していることも明らかになった.我々は,根端におけるRGFの拡散分布のパターンが,PLTの局在とよく一致することから,細胞外に分泌されたRGFがPLTの発現調節を介して根の幹細胞の維持や細胞分裂活性を制御していると考えている.

Report

(5 results)
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (50 results)

All 2010 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 8 results) Presentation (28 results) Book (5 results) Remarks (4 results)

  • [Journal Article] Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis.2010

    • Author(s)
      Matsuzaki Y., et al.
    • Journal Title

      Science

      Volume: 329 Pages: 1065-1067

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis.2009

    • Author(s)
      Komori, R., Amano, Y., Ogawa-Ohnishi, M., Matsubayashi, Y.
    • Journal Title

      Proc Natl Acad Sci USA 106

      Pages: 15067-15072

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana.2009

    • Author(s)
      Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M., Matsubayashi, Y.
    • Journal Title

      Nature Chem Biol 5

      Pages: 578-580

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis2009

    • Author(s)
      Komori R., et. al.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 106 Pages: 15067-15072

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana2009

    • Author(s)
      Ohyama K., et. al.
    • Journal Title

      Nature Chem. Biol.

      Volume: 5 Pages: 578-580

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain.2008

    • Author(s)
      Ogawa, M., Shinohara, H., Sakagami, Y., Matsubayashi, Y.
    • Journal Title

      Science 319

      Pages: 294-294

    • NAID

      130006995152

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Journal Title

      Plant J. 55

      Pages: 152-160

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system.2008

    • Author(s)
      Hirakawa Y., et al.
    • Journal Title

      Proc.Acad.Natl.Sci.USA 105

      Pages: 15208-15213

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. J2008

    • Author(s)
      Ogawa M., et. al.
    • Journal Title

      Science 319

      Pages: 294-294

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis.2007

    • Author(s)
      Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M., Matsubayashi, Y.
    • Journal Title

      Proc Natl Acad Sci USA 104

      Pages: 18333-18338

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing.2007

    • Author(s)
      Shinohara, H., Matsubayashi, Y.
    • Journal Title

      Plant J 52

      Pages: 175-184

    • NAID

      130006995151

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeadstowards receptor array construction and receptor-based ligand fishing.2007

    • Author(s)
      Shinohara H., et. al.
    • Journal Title

      Plant J. 52

      Pages: 175-184

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis.2007

    • Author(s)
      Amano Y., et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 18333-18338

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物の成長制御に関与するペプチドホルモン群に関する研究2010

    • Author(s)
      松林嘉克
    • Organizer
      第8回日本分子生物学会三菱化学奨励賞受賞講演
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 翻訳後修飾に着目した新規ペプチドホルモン探索2010

    • Author(s)
      松林嘉克
    • Organizer
      第62回日本生物工学会大会シンポジウム
    • Place of Presentation
      宮崎シーガイア(宮崎県)
    • Year and Date
      2010-10-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Physiological importance of posttranslational modifications in peptide hormone signaling2010

    • Author(s)
      Yoshikatsu Matsubayashi
    • Organizer
      20th International Conference on Plant Growth Substances
    • Place of Presentation
      Tarragona (Spain)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Secreted Peptide Signals Required for Maintenance of Root Stem Cell Niche in Arabidopsis2010

    • Author(s)
      Yoshikatsu Matsubayashi
    • Organizer
      The 2nd NIBB-MPIPZ Joint Symposium "Plant Science Communications 2010"
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 根端メリステム活性を制御する新規ペプチドホルモン2010

    • Author(s)
      松崎曜, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペプチドホルモンの生理機能に必須な2つの翻訳後修飾:チロシンの硫酸化とプロリンのアラビノシル化2010

    • Author(s)
      松林嘉克
    • Organizer
      第51回日本植物生理学会年会シンポジウム
    • Place of Presentation
      熊本大学(熊本県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 翻訳後修飾に着目した新規ペプチドホルモン候補探索2009

    • Author(s)
      松林嘉克
    • Organizer
      日本化学会第89春期年会シンポジウム"ケミカルバイオロジー研究の最前線"
    • Place of Presentation
      船橋
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] In silico遺伝子スクリーニングによって見出された新規ペプチドホルモン候補2009

    • Author(s)
      松崎曜, ら
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] ペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり2009

    • Author(s)
      松林嘉克
    • Organizer
      第82回日本生化学会大会トップランナーズレクチャー
    • Place of Presentation
      神戸
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Active CLV3 is an arabinosylated glycopeptide2009

    • Author(s)
      Shinohara H., et. at.
    • Organizer
      20th International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh(UK).
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり2009

    • Author(s)
      松林嘉克
    • Organizer
      第82回日本生化学会大会トップランナーズレクチャー
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物茎頂分裂組織における幹細胞の増殖を制御するグリコペプチド2009

    • Author(s)
      松林嘉克
    • Organizer
      第82回日本生化学会大会シンポジウム
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物培養細胞リソースを用いたペプチドホルモン探索2008

    • Author(s)
      松林嘉克
    • Organizer
      文部科学省ナショナルバイオリソースプロジェクトシンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナにおけるチロシン硫酸化酵素の精製2008

    • Author(s)
      小森領太, ら
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] リガンド-受容体ペアの同定から迫る植物のかたちづくり2008

    • Author(s)
      松林嘉克
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 分泌型ペプチドの選択的抽出・解析法と新規ペプチドホルモン探索への応用2008

    • Author(s)
      松林嘉克
    • Organizer
      植物ホルモン技術ワークショップ
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Novel approaches to identify peptide hormone candidates in Arabidopsis.2008

    • Author(s)
      Matsubayashi Y.
    • Organizer
      55th NIBB Conference "Frontiers of Plant Science in the 21st Century"
    • Place of Presentation
      岡崎
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Organizer
      19th International Conference on Arabidopsis Research
    • Place of Presentation
      カナダ,モントリオール
    • Year and Date
      2008-07-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification of a biologically active small secreted peptide in Arabidopsis by in silico gene screening followed by LC-MS-based structure analysis.2008

    • Author(s)
      Ohyama K., et al.
    • Organizer
      Japan-Korea Symposium "Plant Growth and Signal Transduction"
    • Place of Presentation
      横浜
    • Year and Date
      2008-06-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネ細胞培養液に含まれる分泌型ペプチドの解析2008

    • Author(s)
      小木綾子, ら
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受容体の固相固定化を基盤とした新しいリガンド-受容体相互作用検出系2008

    • Author(s)
      篠原秀文, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] CLV3ペプチドはCLV1受容体キナーゼ細胞外領域に直接結合する2008

    • Author(s)
      小川真理, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] 植物細胞培養液を用いた分泌型ペプチドミクス2008

    • Author(s)
      大山健太郎, ら
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.2008

    • Author(s)
      Matsubayashi, Y., Ohyama, K., Ogawa, M.
    • Organizer
      Japan-Korea Symposium - Plant Growth and Signal Transduction.
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 分泌因子および受容体キナーゼを介した情報伝達機構2007

    • Author(s)
      松林嘉克
    • Organizer
      特定領域研究「植物メリステム」公開シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2007-10-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] Roles of two structurally distinct sulfated peptides, PSK and PSY1, in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      19th IPGSA meeting
    • Place of Presentation
      メキシコ,プエルトバジャルタ
    • Year and Date
      2007-07-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      18th International Conference on Arabidopsis Research.
    • Place of Presentation
      中国,北京
    • Year and Date
      2007-06-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development.2007

    • Author(s)
      Matsubayashi Y.
    • Organizer
      18th International Conference on Arabidopsis Research.
    • Place of Presentation
      Beijing, China
    • Related Report
      2009 Self-evaluation Report
  • [Book] 新しい植物ホルモンの科学 第2版 第9章 ペプチドホルモン2010

    • Author(s)
      松林嘉克
    • Total Pages
      215
    • Publisher
      講談社
    • Related Report
      2010 Annual Research Report
  • [Book] 植物のシグナル伝達-分子と応答-分泌型ペプチドホルモンの構造的特徴と受容体相互作用2010

    • Author(s)
      松林嘉克
    • Total Pages
      238
    • Publisher
      共立出版
    • Related Report
      2010 Annual Research Report
  • [Book] 第9章ペプチドホルモン.新しい植物ホルモンの科学2010

    • Author(s)
      松林嘉克
    • Publisher
      講談社
    • Related Report
      2009 Self-evaluation Report
  • [Book] ペプチペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり.蛋白質核酸酵素2009

    • Author(s)
      松林嘉克
    • Related Report
      2009 Self-evaluation Report
  • [Book] リガンド-受容体ペアの解析から探る植物の細胞間シグナリング.化学と生物2008

    • Author(s)
      松林嘉克, 篠原秀文
    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/ligand/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/ligand/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~bioact/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~bioact/

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2018-03-28  

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