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Developmental phase-specific periodicity and the interaction between the circadian clock and cell fate determination.

Planned Research

Project AreaIntrinsic periodicity of cellular systems and its modulation as the driving force behind plant development
Project/Area Number 19H05674
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Endo Motomu  奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (80551499)

Co-Investigator(Kenkyū-buntansha) 波間 茜 (久保田茜)  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (70835371)
Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2024)
Budget Amount *help
¥75,140,000 (Direct Cost: ¥57,800,000、Indirect Cost: ¥17,340,000)
Fiscal Year 2023: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2022: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2021: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2020: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2019: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Keywords概日時計 / 細胞運命決定 / 長距離シグナル伝達 / 細胞解析 / シロイヌナズナ / 周期と変調 / 1細胞解析
Outline of Research at the Start

これまでの1細胞トランスクリプトームにおける空間解像度の問題を解決するために3次元1細胞トランスクリプトームの系を確立するとともに、申請者らが開発した時系列データの再構成技術と組み合わせることで4次元1細胞トランスクリプトームを可能にし、細胞運命決定における周期的な遺伝子発現動態が持つ意味を明らかにする。

Outline of Final Research Achievements

We investigated the role of the circadian clock in plant development and cell elongation control, and revealed a novel phenomenon in which root hair length is periodically regulated by the circadian clock. This rhythmic regulation was completely abolished in the circadian clock mutant toc1. Furthermore, the same loss of rhythmicity was observed when the aerial part of the plant was replaced with a toc1 mutant through grafting, suggesting that a TOC1-dependent signal originating from the shoot is transmitted to the root. While several reports have documented long-distance signaling of circadian rhythms, these have all been limited to regulation at the gene expression level. This study provides the first evidence of long-distance circadian signaling observed at the phenotypic level, demonstrating the transmission of temporal information across plant organs. These findings were published in the journal Plant and Cell Physiology

Academic Significance and Societal Importance of the Research Achievements

概日時計の理解は核内の転写翻訳のフィードバックループから始まり、その後、オルガネラ間の相互作用などより多層的な制御へと枠組みが拡張しつつある。組織間あるいは器官間の時間情報伝達の研究はその延長にあり、植物が個体レベルでどのように時間情報を共有しているかを明らかにするための重要な知見となりうる。
地上部の概日時計が根毛伸長を制御しうるという発見は、葉への部分照射を通じた概日リズムの制御によって栄養や水分の取り込みを制御できる可能性を示唆するものであり、植物工場などでの応用も期待される。

Report

(6 results)
  • 2024 Final Research Report ( PDF )
  • 2023 Annual Research Report
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (29 results)

All 2023 2022 2021

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (20 results) Book (1 results)

  • [Journal Article] Circadian Clock Controls Root Hair Elongation through Long-Distance Communication2023

    • Author(s)
      Ikeda Hikari、Uchikawa Taiga、Kondo Yohei、Takahashi Nozomu、Shishikui Takuma、Watahiki Masaaki K、Kubota Akane、Endo Motomu
    • Journal Title

      Plant And Cell Physiology

      Volume: 64 Issue: 11 Pages: 1289-1300

    • DOI

      10.1093/pcp/pcad076

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The FLOWERING LOCUS T gene expression is controlled by high-irradiance response and external coincidence mechanism in long days in Arabidopsis.2023

    • Author(s)
      Lee Nayoung、Ozaki Yusuke、Hempton Andrew K.、Takagi Hiroshi、Purusuwashi Savita、Song Young Hun、Endo Motomu、Kubota Akane、Imaizumi Takato
    • Journal Title

      New Phytologist

      Volume: - Issue: 1 Pages: 208-221

    • DOI

      10.1111/nph.18932

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Root PRR7 Improves the Accuracy of the Shoot Circadian Clock through Nutrient Transport2023

    • Author(s)
      Uemoto Kyohei、Mori Fumito、Yamauchi Shota、Kubota Akane、Takahashi Nozomu、Egashira Haruki、Kunimoto Yumi、Araki Takashi、Takemiya Atsushi、Ito Hiroshi、Endo Motomu
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 3 Pages: 352-362

    • DOI

      10.1093/pcp/pcad003

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A guiding role of the Arabidopsis circadian clock in cell differentiation revealed by time-series single-cell RNA sequencing2022

    • Author(s)
      Torii Kotaro、Inoue Keisuke、Bekki Keita、Haraguchi Kazuya、Kubo Minoru、Kondo Yuki、Suzuki Takamasa、Kubota Akane、Uemoto Kyohei、Shimizu Hanako、Saito Masato、Fukuda Hiroo、Araki Takashi、Endo Motomu
    • Journal Title

      Cell Reports

      Volume: 40 Issue: 2 Pages: 111059-111059

    • DOI

      10.1016/j.celrep.2022.111059

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sulfanilamide Regulates Flowering Time through Expression of the Circadian Clock Gene LUX2022

    • Author(s)
      Hirohata Atsuhiro、Yamatsuta Yuta、Ogawa Kaori、Kubota Akane、Suzuki Takamasa、Shimizu Hanako、Kanesaka Yuki、Takahashi Nozomu、Endo Motomu
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 5 Pages: 649-657

    • DOI

      10.1093/pcp/pcac027

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Color-Changing Fluorescent Barcode Based on Strand Displacement Reaction Enables Simple Multiplexed Labeling2022

    • Author(s)
      Makino Koki、Susaki Etsuo A.、Endo Motomu、Asanuma Hiroyuki、Kashida Hiromu
    • Journal Title

      Journal of the American Chemical Society

      Volume: 144 Issue: 4 Pages: 1572-1579

    • DOI

      10.1021/jacs.1c09844

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatially specific mechanisms and functions of the plant circadian clock.2022

    • Author(s)
      Davis W, Endo M, Locke JCW.
    • Journal Title

      Plant Physiology

      Volume: -

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Na+/Ca2+ exchanger mediates cold Ca2+ signaling conserved for temperature-compensated circadian rhythms2021

    • Author(s)
      Kon Naohiro、Wang Hsin-tzu、Kato Yoshiaki S.、Uemoto Kyouhei、Kawamoto Naohiro、Kawasaki Koji、Enoki Ryosuke、Kurosawa Gen、Nakane Tatsuto、Sugiyama Yasunori、Tagashira Hideaki、Endo Motomu、Iwasaki Hideo、Iwamoto Takahiro、Kume Kazuhiko、Fukada Yoshitaka
    • Journal Title

      Science Advances

      Volume: 7 Issue: 18 Pages: 1-12

    • DOI

      10.1126/sciadv.abe8132

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 時計遺伝子PRR7は光受容体phototropinのシグナル伝達系を制御する2022

    • Author(s)
      森本隆弘、Carlo F M Gian 粕谷日向子、久保田茜、高橋望、中道範人、遠藤求
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] 栄養素の輸送を介した器官間でのカップリングが概日時計の安定性を高める2022

    • Author(s)
      上本恭平、国本由美、森史、伊藤浩史、江頭春樹、久保田茜、木下俊則、荒木崇、遠藤求
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] Different root expression patterns of circadian clock in Arabidopsis thaliana2022

    • Author(s)
      Yu Leng, Akane Kubota, Nozomu Takahashi, Tatsuaki Goh, Motomu Endo
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] 組織特異的な時計遺伝子の機能解明2022

    • Author(s)
      大畑駿一郎、高橋望、久保田茜、遠藤求
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] B-boxファミリーGroupVは低温下で花成を制御する2022

    • Author(s)
      近藤祐雅、久保田茜、菅野茂夫、村中智明、高橋望、今泉貴登、遠藤求
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] Seasonal cues control the daily expression patterns of FT to optimize flowering time in nature2022

    • Author(s)
      Akane Kubota, Yusuke Ozaki, Yoshinori Kondo, Motomu Endo, Takato Imaizumi
    • Organizer
      第44回分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 時差ボケ実験から植物の外的符合モデルを再考する2021

    • Author(s)
      久保田茜、山本いずみ、遠藤求
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Clock genes show different spatiotemporal expression in Arabidopsis thaliana root2021

    • Author(s)
      Yu Leng, Akane Kubota, Nozomu Takahashi, Tatsuaki Goh, Motomu Endo
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Shoot-root two-way communication stabilizes circadian rhythm in Arabidopsis2021

    • Author(s)
      上本恭平、国本有美、荒木崇、遠藤求
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 地上部の概日時計による根毛伸長メカニズムの解析2021

    • Author(s)
      池田ひかり、内川大雅、近藤洋平、久保田茜、遠藤求
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 野外環境における光と温度によるFT遺伝子の発現制御機構2021

    • Author(s)
      久保田茜、近藤祐雅、遠藤求、今泉貴登
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高温による花成制御機構の解析2021

    • Author(s)
      尾崎友亮、久保田茜、今泉貴登、遠藤求
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Seasonal cues control the daily expression patterns of FT to optimize flowering time in nature2021

    • Author(s)
      Akane Kubota, Yusuke Ozaki, Yoshinori Kondo, Motomu Endo, Takato Imaizumi
    • Organizer
      第44回分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Time to update the external coincidence model in Arabidopsis2021

    • Author(s)
      Motomu Endo
    • Organizer
      Sapporo Symposium on Biological Rhythm 2021
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] 時差ボケ実験から植物の外的符合モデルを再考する2021

    • Author(s)
      久保田茜、山本いずみ、遠藤求
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Clock genes show different spatiotemporal expression in Arabidopsis thaliana root2021

    • Author(s)
      Yu Leng, Akane Kubota, Nozomu Takahashi, Tatsuaki Goh, Motomu Endo
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Shoot-root two-way communication stabilizes circadian rhythm in Arabidopsis2021

    • Author(s)
      上本恭平、国本有美、荒木崇、遠藤求
    • Organizer
      第28回 日本時間生物学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 地上部の概日時計による根毛伸長メカニズムの解析2021

    • Author(s)
      池田ひかり、内川大雅、近藤洋平、久保田茜、遠藤求
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 野外環境における光と温度によるFT遺伝子の発現制御機構2021

    • Author(s)
      久保田茜、近藤祐雅、遠藤求、今泉貴登
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高温による花成制御機構の解析2021

    • Author(s)
      尾崎友亮、久保田茜、今泉貴登、遠藤求
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2019 Annual Research Report
  • [Book] 植物の行動生態学 感じて、伝えて、記憶し、応答する植物たち2023

    • Author(s)
      種生物学会 編 / 山尾 僚・川窪伸光 責任編集
    • Total Pages
      224
    • Publisher
      文一総合出版
    • ISBN
      9784829962091
    • Related Report
      2022 Annual Research Report

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Published: 2019-07-04   Modified: 2026-01-16  

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