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受精卵の周期的動態が非対称性と体軸を生み出す原理の解明

Planned Research

Project AreaIntrinsic periodicity of cellular systems and its modulation as the driving force behind plant development
Project/Area Number 19H05676
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTohoku University

Principal Investigator

植田 美那子  東北大学, 生命科学研究科, 教授 (20598726)

Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2023: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2022: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2021: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2020: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2019: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords植物受精卵 / 細胞伸長 / ライブイメージング / 周期振動 / 植物発生 / 体軸形成 / 周期動態 / 周期変化 / 受精卵 / ライブセルイメージング
Outline of Research at the Start

多細胞生物は複雑な構造をもつが、それら全ては単一細胞である受精卵から発生する。受精卵は非対称に細胞分裂して植物体の上下軸を確立するが、受精卵の内部でどのような変化が生じて非対称分裂に至るのかは、いまだほとんど分かっていない。
そこで本研究では、受精卵内部のダイナミックな変化を網羅的にライブイメージングし、情報科学者らと協力することで、さまざまな事象が示す周期性や、事象間の連動性を見出す。さらに、それぞれの変化を制御する遺伝子群をも特定することで、受精を起点として生じる周期変化が受精卵の非対称分裂や上下軸の形成に果たす役割を明らかにする。

Outline of Annual Research Achievements

受精卵は多細胞生物における個体発生の原点である。しかし植物では、受精卵や初期胚が体軸形成を行う過程において、細胞内でどのような変化が起こるのか、特に、周期的な動態がどのように変調・制御されるのか、ほとんど分かっていなかった。その理由としては、被子植物の花の奥深くに存在する受精卵や胚を生きたまま観察する手法がなかったことと、従来の遺伝学的スクリーニングは、致死性や冗長性のために鍵遺伝子を見出すには不充分だった点が挙げられる。そんななか、研究代表者らはシロイヌナズナを用いて、細胞内動態の詳細なライブイメージングを進めた。特に、精細胞マーカーと卵細胞の細胞膜を特異的に標識した株を掛け合わせ、受精後の動態を詳細にライブイメージングした結果、精細胞と卵細胞が融合した部位に精細胞膜の残骸が持続的に観察され、精細胞侵入点の位置を追跡可能であることを見出した。さらに、受精卵の細胞膜と核を蛍光標識した株についてもライブイメージングを行い、精緻に定量化した結果、受精卵は一定の速度で細胞伸長したあと、一過的に速度を増加させた後に非対称分裂するという、変調を経ることを見出した(Kang et. al., 2023)。加えて、受精後に細胞内で起こるオルガネラ局在や細胞骨格のダイナミックな変化を一元的に比較する方法論も確立したことで、受精卵上部に集積する微小管と、株に移行する液胞が極性形成に重要であることを突き止めた(Hiromoto et. al., 2023)。これらの成果をまとめ、国内外に広く紹介した(Matsumoto and Ueda, 2024)。

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

Report

(5 results)
  • 2023 Annual Research Report
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report

Research Products

(25 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (6 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (4 results)

  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Warwick(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Polarity establishment in the plant zygote at a glance2024

    • Author(s)
      Matsumoto Hikari、Ueda Minako
    • Journal Title

      Journal of Cell Science

      Volume: 137 Issue: 5

    • DOI

      10.1242/jcs.261809

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Coordinate Normalization of Live-Cell Imaging Data Reveals Growth Dynamics of the <i>Arabidopsis</i> Zygote2023

    • Author(s)
      Kang Zichen、Matsumoto Hikari、Nonoyama Tomonobu、Nakagawa Sakumi、Ishimoto Yukitaka、Tsugawa Satoru、Ueda Minako
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 11 Pages: 1279-1288

    • DOI

      10.1093/pcp/pcad020

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive and quantitative analysis of intracellular structure polarization at the apical?basal axis in elongating Arabidopsis zygotes2023

    • Author(s)
      Hiromoto Yukiko、Minamino Naoki、Kikuchi Suzuka、Kimata Yusuke、Matsumoto Hikari、Nakagawa Sakumi、Ueda Minako、Higaki Takumi
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 22879-22879

    • DOI

      10.1038/s41598-023-50020-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species2023

    • Author(s)
      Kimata Yusuke、Yamada Moe、Murata Takashi、Kuwata Keiko、Sato Ayato、Suzuki Takamasa、Kurihara Daisuke、Hasebe Mitsuyasu、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Life Science Alliance

      Volume: 6 Issue: 5 Pages: e202201657-e202201657

    • DOI

      10.26508/lsa.202201657

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Behavior of Male Gamete Fusogen GCS1/HAP2 and the Regulation in Arabidopsis Double Fertilization2023

    • Author(s)
      Shiba Yuka、Takahashi Taro、Ohashi Yukino、Ueda Minako、Mimuro Amane、Sugimoto Jin、Noguchi Yuka、Igawa Tomoko
    • Journal Title

      Biomolecules

      Volume: 13 Issue: 2 Pages: 208-208

    • DOI

      10.3390/biom13020208

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the <i>Arabidopsis</i> Zygote2021

    • Author(s)
      Matsumoto Hikari、Kimata Yusuke、Higaki Takumi、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 8 Pages: 1280-1289

    • DOI

      10.1093/pcp/pcab075

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] What is quantitative plant biology?2021

    • Author(s)
      Autran D., Bassel GW., Chae E., Ezer D., Ferjani A., Fleck C., Hamant O., Hartmann FP., Jiao Y., Johnston IG., Kwiatkowska D., Lim BL., Mahnen AP., Morris RJ., Mulder BM., Nakayama N., Sozzani R., Strader LC., Tusscher K., Ueda M., Wolf S.
    • Journal Title

      Quantitative Plant Biology

      Volume: 2 Pages: 10-10

    • DOI

      10.1017/qpb.2021.8

    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis2021

    • Author(s)
      Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
    • Journal Title

      PLOS Biology

      Volume: 19 Issue: 3 Pages: 3001123-3001123

    • DOI

      10.1371/journal.pbio.3001123

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes2020

    • Author(s)
      Kimata Yusuke、Higaki Takumi、Kurihara Daisuke、Ando Naoe、Matsumoto Hikari、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Quantitative Plant Biology

      Volume: 1

    • DOI

      10.1017/qpb.2020.4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A new role for histone demethylases in the maintenance of plant genome integrity2020

    • Author(s)
      Antunez-Sanchez Javier、Naish Matthew、Ramirez-Prado Juan Sebastian、Ohno Sho、Huang Ying、Dawson Alexander、Opassathian Korawit、Manza-Mianza Deborah、Ariel Federico、Raynaud Cecile、Wibowo Anjar、Daron Josquin、Ueda Minako、Latrasse David、Slotkin R Keith、Weigel Detlef、Benhamed Moussa、Gutierrez-Marcos Jose
    • Journal Title

      eLife

      Volume: 9

    • DOI

      10.7554/elife.58533

    • NAID

      120007179207

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Live-cell imaging of the body axis formation during Arabidopsis embryogenesis.2023

    • Author(s)
      Minako Ueda
    • Organizer
      Taiwan-Japan Plant Biology 2023 (TJPB2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging of body axis formation in plant embryos.2022

    • Author(s)
      Ueda M.
    • Organizer
      Japan-Singapore Joint Meeting 2022
    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Invited
  • [Presentation] Live-cell imaging of the polarization dynamics of plant zygote.2021

    • Author(s)
      Ueda M., Kimata Y., Matsumoto H., Higaki T., Komatsu T., Tanaka S., Kurihara D., Higashiyama T.
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live imaging of asymmetric cell division of plant zygote.2020

    • Author(s)
      Kimata Y., Higaki T., Kurihara D., Higashiyama T., Ueda M.
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging from zygote polarization to embryo patterning in plant.2020

    • Author(s)
      Ueda M., Kimata Y., Tanaka S., Higaki T., Kurihara D., Higashiyama T
    • Organizer
      日本発生生物学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 東北大学生命科学研究科植物細胞動態分野

    • URL

      http://www.lifesci.tohoku.ac.jp/PlantCellDyn/

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 植物の受精卵が形を変えながら伸びることを発見(PCP2023論文の紹介記事)

    • URL

      https://www.lifesci.tohoku.ac.jp/research/results/detail---id-51682.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 植物の卵細胞がつくられる様子を生きたまま観察することに成功

    • URL

      https://www.yokohama-cu.ac.jp/news/2020/202103maruyama_PLOSBiol.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 植物の受精卵が非対称にミトコンドリアを分配する仕組みを発見

    • URL

      https://www.lifesci.tohoku.ac.jp/research/results/detail---id-49726.html

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-07-04   Modified: 2024-12-25  

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