Elucidation of the principles underlying the periodic dynamics in the zygote to generate cell asymmetry and body axis.

• Project Area: Intrinsic periodicity of cellular systems and its modulation as the driving force behind plant development
• Project/Area Number: 19H05676
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: Ueda Minako 東北大学, 生命科学研究科, 教授 (20598726)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000); Fiscal Year 2023: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2022: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2021: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2020: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2019: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 植物受精卵 / 細胞伸長 / ライブイメージング / 周期振動 / 植物発生 / 体軸形成 / 周期動態 / 周期変化 / 受精卵 / ライブセルイメージング

Outline of Research at the Start

多細胞生物は複雑な構造をもつが、それら全ては単一細胞である受精卵から発生する。受精卵は非対称に細胞分裂して植物体の上下軸を確立するが、受精卵の内部でどのような変化が生じて非対称分裂に至るのかは、いまだほとんど分かっていない。
そこで本研究では、受精卵内部のダイナミックな変化を網羅的にライブイメージングし、情報科学者らと協力することで、さまざまな事象が示す周期性や、事象間の連動性を見出す。さらに、それぞれの変化を制御する遺伝子群をも特定することで、受精を起点として生じる周期変化が受精卵の非対称分裂や上下軸の形成に果たす役割を明らかにする。

Outline of Final Research Achievements

The apical-basal axis formation is the origin of ontogeny. In plants, however, the details of this process and how the periodic dynamics are regulated were largely unknown. Therefore, we performed various live imaging analyses using Arabidopsis embryos (Matsumoto et. al., 2021), and found that the growth rate of zygotes is temporally modulated before the cell division (Kang et. al., 2023). We summarized our findings and introduced them widely (Matsumoto and Ueda, 2024).

Academic Significance and Societal Importance of the Research Achievements

研究代表者らは、植物の受精卵が上下に極性化する動態をリアルタイムで観察したことで、細胞内のダイナミックな動きや制御が、上下軸形成の起点である受精卵の不等分裂に重要であることを突き止めた。多くの植物において、受精卵は上下に不等分裂することで植物体の上下軸を確立することから、本研究で見出された動態や仕組みは、植物に共通した原理であると期待される。

Research Products

• [Int'l Joint Research] Wageningen University(オランダ)
• [Int'l Joint Research] Wageningen University(オランダ)
• [Int'l Joint Research] Wageningen University(オランダ)
• [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)
• [Int'l Joint Research] University of Warwick(英国)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Journal Article] Polarity establishment in the plant zygote at a glance (2024)
  • Author(s): Matsumoto Hikari、Ueda Minako
  • Journal Title: Journal of Cell Science Volume: 137 Issue: 5
  • DOI: 10.1242/jcs.261809
• [Journal Article] Coordinate Normalization of Live-Cell Imaging Data Reveals Growth Dynamics of the <i>Arabidopsis</i> Zygote (2023)
  • Author(s): Kang Zichen、Matsumoto Hikari、Nonoyama Tomonobu、Nakagawa Sakumi、Ishimoto Yukitaka、Tsugawa Satoru、Ueda Minako
  • Journal Title: Plant and Cell Physiology Volume: - Issue: 11 Pages: 1279-1288
  • DOI: 10.1093/pcp/pcad020
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive and quantitative analysis of intracellular structure polarization at the apical?basal axis in elongating Arabidopsis zygotes (2023)
  • Author(s): Hiromoto Yukiko、Minamino Naoki、Kikuchi Suzuka、Kimata Yusuke、Matsumoto Hikari、Nakagawa Sakumi、Ueda Minako、Higaki Takumi
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 22879-22879
  • DOI: 10.1038/s41598-023-50020-8
  • Peer Reviewed / Open Access
• [Journal Article] Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species (2023)
  • Author(s): Kimata Yusuke、Yamada Moe、Murata Takashi、Kuwata Keiko、Sato Ayato、Suzuki Takamasa、Kurihara Daisuke、Hasebe Mitsuyasu、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Life Science Alliance Volume: 6 Issue: 5 Pages: e202201657-e202201657
  • DOI: 10.26508/lsa.202201657
  • Peer Reviewed / Open Access
• [Journal Article] Behavior of Male Gamete Fusogen GCS1/HAP2 and the Regulation in Arabidopsis Double Fertilization (2023)
  • Author(s): Shiba Yuka、Takahashi Taro、Ohashi Yukino、Ueda Minako、Mimuro Amane、Sugimoto Jin、Noguchi Yuka、Igawa Tomoko
  • Journal Title: Biomolecules Volume: 13 Issue: 2 Pages: 208-208
  • DOI: 10.3390/biom13020208
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the <i>Arabidopsis</i> Zygote (2021)
  • Author(s): Matsumoto Hikari、Kimata Yusuke、Higaki Takumi、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Plant and Cell Physiology Volume: 62 Issue: 8 Pages: 1280-1289
  • DOI: 10.1093/pcp/pcab075
  • Peer Reviewed / Open Access
• [Journal Article] What is quantitative plant biology? (2021)
  • Author(s): Autran D., Bassel GW., Chae E., Ezer D., Ferjani A., Fleck C., Hamant O., Hartmann FP., Jiao Y., Johnston IG., Kwiatkowska D., Lim BL., Mahnen AP., Morris RJ., Mulder BM., Nakayama N., Sozzani R., Strader LC., Tusscher K., Ueda M., Wolf S.
  • Journal Title: Quantitative Plant Biology Volume: 2 Pages: 10-10
  • DOI: 10.1017/qpb.2021.8
  • Int'l Joint Research
• [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis (2021)
  • Author(s): Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
  • Journal Title: PLOS Biology Volume: 19 Issue: 3 Pages: 3001123-3001123
  • DOI: 10.1371/journal.pbio.3001123
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes (2020)
  • Author(s): Kimata Yusuke、Higaki Takumi、Kurihara Daisuke、Ando Naoe、Matsumoto Hikari、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Quantitative Plant Biology Volume: 1
  • DOI: 10.1017/qpb.2020.4
  • Peer Reviewed / Open Access
• [Journal Article] A new role for histone demethylases in the maintenance of plant genome integrity (2020)
  • Author(s): Antunez-Sanchez Javier、Naish Matthew、Ramirez-Prado Juan Sebastian、Ohno Sho、Huang Ying、Dawson Alexander、Opassathian Korawit、Manza-Mianza Deborah、Ariel Federico、Raynaud Cecile、Wibowo Anjar、Daron Josquin、Ueda Minako、Latrasse David、Slotkin R Keith、Weigel Detlef、Benhamed Moussa、Gutierrez-Marcos Jose
  • Journal Title: eLife Volume: 9
  • DOI: 10.7554/elife.58533
  • NAID: 120007179207
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Live-cell imaging of the body axis formation during Arabidopsis embryogenesis. (2023)
  • Author(s): Minako Ueda
  • Organizer: Taiwan-Japan Plant Biology 2023 (TJPB2023)
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging of body axis formation in plant embryos. (2022)
  • Author(s): Ueda M.
  • Organizer: Japan-Singapore Joint Meeting 2022
  • Invited
• [Presentation] Live-cell imaging of the polarization dynamics of plant zygote. (2021)
  • Author(s): Ueda M., Kimata Y., Matsumoto H., Higaki T., Komatsu T., Tanaka S., Kurihara D., Higashiyama T.
  • Organizer: 第44回日本分子生物学会年会
  • Int'l Joint Research / Invited
• [Presentation] Live imaging of asymmetric cell division of plant zygote. (2020)
  • Author(s): Kimata Y., Higaki T., Kurihara D., Higashiyama T., Ueda M.
  • Organizer: 第43回日本分子生物学会年会
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging from zygote polarization to embryo patterning in plant. (2020)
  • Author(s): Ueda M., Kimata Y., Tanaka S., Higaki T., Kurihara D., Higashiyama T
  • Organizer: 日本発生生物学会
  • Invited
• [Remarks] 東北大学生命科学研究科植物細胞動態分野
  • URL: http://www.lifesci.tohoku.ac.jp/PlantCellDyn/
• [Remarks] 植物の受精卵が形を変えながら伸びることを発見（PCP2023論文の紹介記事）
  • URL: https://www.lifesci.tohoku.ac.jp/research/results/detail---id-51682.html
• [Remarks] 植物の卵細胞がつくられる様子を生きたまま観察することに成功
  • URL: https://www.yokohama-cu.ac.jp/news/2020/202103maruyama_PLOSBiol.html
• [Remarks] 植物の受精卵が非対称にミトコンドリアを分配する仕組みを発見
  • URL: https://www.lifesci.tohoku.ac.jp/research/results/detail---id-49726.html