| Budget Amount *help |
¥93,730,000 (Direct Cost: ¥72,100,000、Indirect Cost: ¥21,630,000)
Fiscal Year 2023: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
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| Outline of Final Research Achievements |
To develop a "post-Koch" technique that allows for nondestructive analysis of the species and functions of unknown microbes, we combined Raman-based molecular spectroscopic techniques with machine learning and gel-filled microarray devices. Using machine learning of single-cell Raman data, we achieved highly accurate species- and domain-level classification of bacteria and archaea. We demonstrated and established protocols in which soil microbes are cultured on newly developed gel-filled microarray devices, subjected to Raman measurements, and picked up for further analysis. We also measured, for the first time to our knowledge, characteristic Raman spectra of methanogenic archaea and rare actinomycetes.
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