| Budget Amount *help |
¥90,740,000 (Direct Cost: ¥69,800,000、Indirect Cost: ¥20,940,000)
Fiscal Year 2023: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
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| Outline of Final Research Achievements |
The advent of high-throughput “omics” technology has substantially expanded our knowledge of microbial diversity. Despite advancements in sequencing technologies, most microorganisms remain unculturable in the laboratory. To explore such unseen microorganisms beyond their genes/genomes, we have developed novel cultivation strategies in the scientific project, “Post-Koch Ecology.” Our group aimed to elucidate “cultivation tips” to improve the cultivation efficiency of these difficult-to-culture bacteria. We isolated approximately 500 phylogenetically novel bacterial strains from upland soils under various media conditions. Based on the efficacy of each tested culture medium, we designed new cultivation conditions and applied a gel-filled microwell array device as a novel cultivation technology for high-throughput mining of uncultured bacteria. The novel strains were deposited at the RIKEN BRC as bioresources and made available for future academic and industrial research.
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