| Budget Amount *help |
¥140,010,000 (Direct Cost: ¥107,700,000、Indirect Cost: ¥32,310,000)
Fiscal Year 2023: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2022: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2021: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2020: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2019: ¥34,970,000 (Direct Cost: ¥26,900,000、Indirect Cost: ¥8,070,000)
|
|---|
| Outline of Final Research Achievements |
We developed a method for isolating rare actinomycetes using microculture arrays, including a co-culture system. Eight hundred and sixty-eight strains of rare actinomycetes were isolated, including 124 strains with high novelty, mainly using a modified conventional method. The genome sequences of six isolates were determined and used for genome mining. Twenty-nine isolates were subjected to PC screening and three novel compounds were identified. The biosynthetic pathways of seven actinomycete secondary metabolites were elucidated, and many enzymes that catalyze novel reactions were identified. We elucidated many of the molecular bases for the unique morphological and physiological characteristics exhibited by the model rare actinomycete Actinoplanes missouriensis. We also initiated a new study on rare actinomycetes in the genus Sporichthya.
|
