| Budget Amount *help |
¥135,850,000 (Direct Cost: ¥104,500,000、Indirect Cost: ¥31,350,000)
Fiscal Year 2023: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2022: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2021: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2020: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2019: ¥34,970,000 (Direct Cost: ¥26,900,000、Indirect Cost: ¥8,070,000)
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| Outline of Final Research Achievements |
In order to analyze the interactions between the pollutant-degrading bacteria and surrounding bacteria under the natural environmental condition, we developed Post-Koch techniques based on the “isolated cultivation” and/or “single-cell inoculation”. By using these, we were able to easily obtain a diverse group of degradative bacteria and easily detect interactions that affect their function. We also applied the developed techniques to detect interactions between important bacteria isolated from the model field soil and other bacteria.
Meanwhile, we obtained a large number of different plasmids from model fields. Based on their nucleotide sequences and basic functions (conjugation and replication host ranges), we classified the isolated plasmids and discussed the plasmid dynamics in model field environments. We also developed a method to predict conjugation host range and compiled a high-precision plasmid database.
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