| Budget Amount *help |
¥255,580,000 (Direct Cost: ¥196,600,000、Indirect Cost: ¥58,980,000)
Fiscal Year 2023: ¥58,890,000 (Direct Cost: ¥45,300,000、Indirect Cost: ¥13,590,000)
Fiscal Year 2022: ¥57,330,000 (Direct Cost: ¥44,100,000、Indirect Cost: ¥13,230,000)
Fiscal Year 2021: ¥56,030,000 (Direct Cost: ¥43,100,000、Indirect Cost: ¥12,930,000)
Fiscal Year 2020: ¥55,640,000 (Direct Cost: ¥42,800,000、Indirect Cost: ¥12,840,000)
Fiscal Year 2019: ¥27,690,000 (Direct Cost: ¥21,300,000、Indirect Cost: ¥6,390,000)
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| Outline of Final Research Achievements |
Liver-specific selective autophagy-incompetent mice were generated by utilizing a probe that specifically binds to the membrane-bound ATG8 family. We found that overexpression of NBR1, a binding partner of p62, decreased the fluidity of p62 bodies while increasing their number and size. We established a method for highly efficient purification of fluorescently labeled p62 bodies using a cell sorter. Integrative proteomic analysis of p62 bodies purified from NBR1 overexpressing cells and liver-specific selective autophagy-incompetent mouse liver identified a novel selective autophagy substrates that localize to p62 bodies and are degraded by autophagy.
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