| Budget Amount *help |
¥168,740,000 (Direct Cost: ¥129,800,000、Indirect Cost: ¥38,940,000)
Fiscal Year 2023: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2022: ¥31,850,000 (Direct Cost: ¥24,500,000、Indirect Cost: ¥7,350,000)
Fiscal Year 2021: ¥33,670,000 (Direct Cost: ¥25,900,000、Indirect Cost: ¥7,770,000)
Fiscal Year 2020: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Fiscal Year 2019: ¥43,160,000 (Direct Cost: ¥33,200,000、Indirect Cost: ¥9,960,000)
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| Outline of Final Research Achievements |
Inheritance of non-genomic information is essential for inheritance of cellular phenotype. On the other hand, such non-genomic information has plasticity and can be re-wired, thereby it functions as the basic principle for generating diverse cell types. Elucidation of the mechanisms that control the modification and precise replication of non-genomic information is an important issue in biology. In this research project, we have designed researches to elucidate the mechanisms of replication of non-genomic information, by using hematopoietic cell differentiation as a model. We identified Dot1L, Runx3, and Ambra1 as a molecule essential for the maintenance of Cd8 gene expression by screening gRNA libraries.
We established the in vivo AID2 system in mice for drug-inducible, temporal degradation of target proteins as a new research method for identification and functional analysis of nuclear proteins involved in the formation and maintenance of higher-order chromatin structures.
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