| Budget Amount *help |
¥100,490,000 (Direct Cost: ¥77,300,000、Indirect Cost: ¥23,190,000)
Fiscal Year 2023: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2020: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2019: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
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| Outline of Final Research Achievements |
Using mouse ES cells, we analyzed the mechanism by which transcription factor network activity regulating gene expression is inherited through cell division. The repli-ATAC-seq analysis of transcription factor binding to the S-phase nascent strand revealed that KLF2/4/5-mediated activation of distal enhancer and the resulting change in accessibility of the promoter region associated with the change in gene expression play an important role in the restructuring of transcriptional network activity after DNA replication. We also identified Dppa2 as a novel M-phase chromosome-binding transcription factor, which antagonizes the PCGF6-PRC1 polycomb group complex to regulate novel methylation of target genes.
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