| Budget Amount *help |
¥104,780,000 (Direct Cost: ¥80,600,000、Indirect Cost: ¥24,180,000)
Fiscal Year 2023: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2022: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2021: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2020: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2019: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
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| Outline of Final Research Achievements |
Result 1: Many animals have a conserved adaptive genome defence system known as the Piwi-interacting RNA (piRNA) pathway, which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes play little or no role in mammalian oocytes. With an improved high-quality genome assembly and annotation of golden hamster, we were able to generate PIWI-defective golden hamsters, which have defects in the production of functional oocytes.
Result 2: Zygotic genome activation (ZGA) is a critical step that promotes totipotency. MERVL is transiently upregulated during ZGA. Although MERVL expression is widely used as a marker of totipotency, the role of this retrotransposon in mouse embryogenesis remained elusive. We were able to demonstrate that MERVL expression is essential for accurate regulation of the host transcriptome and chromatin state during preimplantation development.
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