| Budget Amount *help |
¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000)
Fiscal Year 2023: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
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| Outline of Final Research Achievements |
In a fertilized egg of animals, the regulatory mode of the gamete genome is converted to an early embryo-specific one, thus allowing differentiation into various cells in the whole body. In this study, we have examined mechanistically how factors derived from egg cytoplasm promote nuclear reprogramming. Our attempt to modify cell-free assays of frog egg extracts revealed the role of proteins essential for zygotic genome activation and somatic cell nuclear transfer. We also established the protocols for in vitro reconstitution of these processes using purified factors. In sum, these results undoubtedly broaden our biochemical understanding of the process of totipotency acquisition.
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