| Budget Amount *help |
¥91,780,000 (Direct Cost: ¥70,600,000、Indirect Cost: ¥21,180,000)
Fiscal Year 2023: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
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| Outline of Final Research Achievements |
Fertilized eggs formed by the fusion of sperm and egg possesses totipotency. Zygotic genome activation (ZGA) occurring in the fertilized egg represents a unique transcriptional program, strongly suggesting the importance of significant epigenomic rewriting, termed epigenome reprogramming, for its initiation. This study aimed to gain a detailed understanding of the molecular mechanisms controlling epigenetic reprogramming and ZGA in fertilized eggs. We revealed (1) the atypical distribution of the histone variant H3.3 during totipotency, (2) the establishment of genome-wide nucleosome positioning during post-fertilization embryonic development, and (3) the occurrence of dynamic transcriptional reprogramming during the ZGA period.
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