| Budget Amount *help |
¥80,210,000 (Direct Cost: ¥61,700,000、Indirect Cost: ¥18,510,000)
Fiscal Year 2023: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2022: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2021: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2020: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2019: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
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| Outline of Final Research Achievements |
Iron is a bio-metal, which is essential in maintaining the lives of all living system. In this project, we have studies the dynamics of iron in cell (absorption, sensing, transport, storage, usage etc) on the basis of the dynamic structures and interaction of proteins related to the iron dynamics.
In human, the iron in diet is absorbed in duodenum, in which iron reductase (dcytb) and iron transporter (DMT1) play crucial roles. We studies the interaction of Dcytb with the iron by native MASS technique, and with DMT1 in cell to transport the ferrous iron resulted by the reduction of ferric iron by Dcytb by PLA assay.
We also studied the molecular mechanisms of heme inporter, heme sensor protein, and heme exporter in pothogenic bacteria, all of which play crucial roles in acquisition of iron. Especially, we focused on their dynamic structures in functionalization.
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