In-cell NMR studies of metalloproteins

• Project Area: Integrated Biometal Science: Research to Explore Dynamics of Metals in Cellular System
• Project/Area Number: 19H05773
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: 伊藤 隆 東京都立大学, 理学研究科, 教授 (80261147)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥78,130,000 (Direct Cost: ¥60,100,000、Indirect Cost: ¥18,030,000); Fiscal Year 2023: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2022: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2021: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2019: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
• Keywords: in-cell NMR / 金属結合タンパク質 / 立体構造 / 溶液NMR / 常磁性NMR

Outline of Research at the Start

細胞内は多種多様な分子が高密度で存在する「分子クラウディング」状態である．金属結合タンパク質の動態についても，細胞内では，クラウディング環境特有の相互作用（排除体積効果・非特異的相互作用）に加え，様々な「生命金属」イオンとの結合や，機能発現に関わる他の因子との特異的相互作用により強い影響を受けていると予想される．
細胞試料を直接NMR測定するin-cell NMRは，生細胞中タンパク質の動態を原子分解能で解析可能な現存する唯一の手法である．本研究は，in-cell NMRを用いて，金属結合タンパク質が関与する生命現象のin situ解析を行うものである．

Outline of Annual Research Achievements

様々な金属結合タンパク質についてin-cell NMR解析を行うための要素技術の研究を行っている．2023年度は特に，in-cell NMR解析技術の開発と，複合体形成やドメイン間相対配置の解析に資する常磁性NMR解析法の開発を行った．
「in-cell NMR解析技術の開発」については，不安定なタンパク質のin-cell NMR解析の研究を行った．Intrinsically Disordered Proteins (IDPs)はin-cell NMRの重要な標的であるが，細胞内で分解されるために解析が困難になる場合がある．様々なIDP試料の良好なin-cell NMRスペクトル取得を目指した．モデル試料として，KEAP1/NRF2系を用いた．NRF2は酸化還元ストレスに対応して，抗酸化酵素，金属結合タンパク質などの遺伝子の転写を制御している．NRF2のKEAP1結合領域（NRF2-NEH2）はIDPとしてふるまい，KEAP1存在下でユビキチン化されてプロテアソームで分解されるため，in-cell NMR解析が不可能であった．プロテアソーム阻害剤MG-132存在下でHeLa細胞を用いたNRF2-NEH2のin-cell NMR測定を行った結果良好なスペクトルが得られた．また内在性KEAP1との相互作用に由来するシグナルのブロードニングも観測した．MG-132は細胞内で不安定な他のタンパク質のin-cell NMR測定でも有効であった．
「複合体形成やドメイン間相対配置の解析に資する常磁性NMR解析法の開発」については，NMRによるFixL-FixJ二成分シグナル伝達系の構造解析を継続して進めた．ドメインの立体構造はNOE由来の距離情報を，ドメイン間相対配置は常磁性NMR情報を用いて立体構造計算を行った結果，X線結晶解析結果とは大きく異なる溶液構造を決定することができた．

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Cambridge/University of Leicester(英国)
• [Int'l Joint Research] Goethe University Frankfurt(ドイツ)
• [Int'l Joint Research] ETH Zurich(スイス)
• [Int'l Joint Research] University of Cambridge/University of Leicester(英国)
• [Int'l Joint Research] Goethe University Frankfurt(ドイツ)
• [Int'l Joint Research] ETH Zurich(スイス)
• [Int'l Joint Research] University of Cambridge/University of Leicester(英国)
• [Int'l Joint Research] Goethe University Frankfurt(ドイツ)
• [Int'l Joint Research] ETH Zurich(スイス)
• [Int'l Joint Research] University of Cambridge/University of Leicester(英国)
• [Int'l Joint Research] Goethe University Frankfurt(ドイツ)
• [Int'l Joint Research] ETH Zurich(スイス)
• [Int'l Joint Research] University of Cambridge/University of Leicester(英国)
• [Int'l Joint Research] Goethe University Frankfurt(ドイツ)
• [Int'l Joint Research] ETH Zurich(スイス)
• [Journal Article] Direct Observation of Membrane-Associated H-Ras in the Native Cellular Environment by In-Cell ¹⁹F-NMR Spectroscopy (2023)
  • Author(s): Ikari Masaomi、Yagi Hiromasa、Kasai Takuma、Inomata Kohsuke、Ito Masahiro、Higuchi Kae、Matsuda Natsuko、Ito Yutaka、Kigawa Takanori
  • Journal Title: JACS Au Volume: 3 Issue: 6 Pages: 1658-1669
  • DOI: 10.1021/jacsau.3c00108
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural insights into the complex of oncogenic KRas4BG12V and Rgl2, a RalA/B activator (2023)
  • Author(s): M. Tariq, T. Ikeya, N. Togashi, L. Fairall, S. Kamei, S. Mayooramurugan, L.R. Abbott, A. Hasan, C. Bueno-Alejo, S. Sukegawa, B. Romartinez-Alonso, M.A.M. Campillo, A.J. Hudson, Y. Ito, J.W.R. Schwabe, C. Dominguez, K. Tanaka
  • Journal Title: Life Sci. Alliance Volume: 7 Issue: 1 Pages: e202302080-e202302080
  • DOI: 10.26508/lsa.202302080
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Interactions of the N- and C-Terminal SH3 Domains of Drosophila Drk with the Proline-Rich Peptides from Sos and Dos (2023)
  • Author(s): P.M. Sayeesh, M. Iguchi, Y. Suemoto, J. Inoue, K. Inomata, T. Ikeya, Y. Ito
  • Journal Title: Int. J. Mol. Sci. Volume: 24 Issue: 18 Pages: 14135-14135
  • DOI: 10.3390/ijms241814135
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and biochemical insights into heterotetramer formation of oncogenic K-Ras4BG12V and Rgl2, a RalA/B activator (2023)
  • Author(s): 02.Tariq, M., Ikeya, T., Togashi, N., Fairall, L., Bueno-Alejo, C., Kamei, S., Romartinez-Alonso, B., Campillo, M.A.M., Hudson, A. J., Ito, Y., Schwabe, J.W.R., Dominguez, C. & Tanaka, K.
  • Journal Title: bioRxiv Volume: 1 Pages: 1-56
  • DOI: 10.1101/2022.10.10.511529
  • Open Access / Int'l Joint Research
• [Journal Article] Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos (2022)
  • Author(s): Sayeesh P.M., Ikeya, T., Sugasawa, H., Watanabe, R., Mishima, M., Inomata, K., & Ito, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 625 Pages: 87-93
  • DOI: 10.1016/j.bbrc.2022.08.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural Biology Studies to Understand the Mechanism of SARS-CoV-2 Infection (2021)
  • Author(s): 伊藤 隆
  • Journal Title: Earozoru Kenkyu Volume: 36 Issue: 4 Pages: 237-245
  • DOI: 10.11203/jar.36.237
  • NAID: 130008132519
  • ISSN: 0912-2834, 1881-543X
  • Year and Date: 2021-12-20
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE (2021)
  • Author(s): Takayuki Nagae, Masashi Unno, Taiki Koizumi, Yohei Miyanoiri, Tomotsumi Fujisawa, Kento Masui, Takanari Kamo, Kei Wada, Toshihiko Eki, Yutaka Ito, Yuu Hirose, Masaki Mishima
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118(20) Issue: 20
  • DOI: 10.1073/pnas.2024583118
  • Peer Reviewed / Open Access
• [Journal Article] Multi-state structure determination and dynamics analysis reveals a new ubiquitin-recognition mechanism in yeast ubiquitin C-terminal hydrolase (2021)
  • Author(s): Okada, M., Tateishia, Y., Nojiri, E., Mikawa, T., Rajesh, S., Ogasa, H., Ueda, T., Yagi, H., Kohno, T., Kigawa, T., Shimada, I., Guentert, P., Ito, Y., & Ikeya, T.
  • Journal Title: biorxiv Volume: 2021.04.22.440356 Pages: 1-39
  • DOI: 10.1101/2021.04.22.440356
  • Open Access / Int'l Joint Research
• [Journal Article] 溶液NMR法を用いた蛋白質立体構造計算の最近の動向 (2021)
  • Author(s): 池谷鉄兵，伊藤 隆
  • Journal Title: 細胞 Volume: 53 Pages: 48-52
  • NAID: 40022675452
  • Peer Reviewed
• [Journal Article] 溶液NMR法を用いた蛋白質立体構造計算の最近の動向 (2021)
  • Author(s): 池谷鉄兵，伊藤 隆
  • Journal Title: アグリバイオ Volume: 5 Pages: 980-986
  • NAID: 40022675452
  • Peer Reviewed
• [Journal Article] Domain selective labeling for NMR studies of multidomain proteins by domain ligation using highly active sortase A (2020)
  • Author(s): Aizu Takahiro、Suzuki Takumi、Kido Akihiro、Nagai Kan、Kobayashi Ayaho、Sugiura Reiko、Ito Yutaka、Mishima Masaki
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1864 Issue: 2 Pages: 129419-129419
  • DOI: 10.1016/j.bbagen.2019.129419
  • Peer Reviewed
• [Journal Article] In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli (2020)
  • Author(s): Sugiki Toshihiko、Yamaguchi Yoshihiro、Fujiwara Toshimichi、Inouye Masayori、Ito Yutaka、Kojima Chojiro
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 2466-2466
  • DOI: 10.1038/s41598-020-59076-2
  • NAID: 120006952208
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In situ structural biology using in-cell NMR (2020)
  • Author(s): Nishida Noritaka、Ito Yutaka、Shimada Ichio
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1864 Issue: 2 Pages: 129364-129364
  • DOI: 10.1016/j.bbagen.2019.05.007
  • Peer Reviewed
• [Journal Article] High resolution protein 3D structure determination in living eukaryotic cells (2019)
  • Author(s): Tanaka, T., Ikeya, T., Kamoshida, H., Suemoto, Y., Mishima, M., Shirakawa, M., Guentert, P., Ito, Y.
  • Journal Title: Angewandte Chemie International Edition Volume: in press Issue: 22 Pages: 1-7
  • DOI: 10.1002/anie.201900840
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protein Structure Determination in Living Cells. (2019)
  • Author(s): Teppei Ikeya, Peter Guentert, Yutaka Ito
  • Journal Title: international journal of molecular sciences Volume: 20 Issue: 10 Pages: 2442-2442
  • DOI: 10.3390/ijms20102442
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] NMR studies of multi-domain adaptor proteins in signal transduction pathways (2024)
  • Author(s): Yutaka Ito，Teppei Ikeya，Sayeesh P.M.，Mao Oide，Yuji Sugita, Hiroshima
  • Organizer: TJ2024 (Taiwan-Japan NMR Symposium 2024)
  • Int'l Joint Research / Invited
• [Presentation] In-cell NMRを用いたKeap1-Nrf2系の解析 (2024)
  • Author(s): 伊藤 隆
  • Organizer: NMRシンポジウム―環境応答の構造生物学
  • Invited
• [Presentation] In-cell NMR studies of the Keap1-Nrf2 system (2023)
  • Author(s): Yutaka Ito，Mei Toyoda，Shiori Suehiro，Kaito Shima，Teppei Ikeya，Takafumi Suzuki，Masayuki Yamamoto，Kohsuke Inomata
  • Organizer: Asia-Pacific Nuclear Magnetic Resonance (APNMR) 2023
  • Int'l Joint Research / Invited
• [Presentation] 多次元NMRの原理 (2023)
  • Author(s): 伊藤 隆
  • Organizer: 2023年度?本分光学会主催秋期セミナー「NMR分光法の基礎と応用」
  • Invited
• [Presentation] Analysis of the mechanism underlying multivalent interactions between GRB2 and SOS1 and their LLPS using solution NMR (2023)
  • Author(s): Teppei Ikeya，Mao Oide，W. Ren，Keita Tateno，Takami Ando，Haruka Sugasawa，Yuji Sugita，Yutaka Ito
  • Organizer: 日本生物物理学会第61回年会
• [Presentation] GRB2とSOS1による多価相互作用と液液相分離の溶液NMR解析 (2023)
  • Author(s): 池谷鉄兵，菅澤はるか，富樫直之，林 俊文，美川 務，杉田有治，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] In-cell NMRにおける細胞試料管理 (2023)
  • Author(s): 猪股晃介，豊田芽生，島 海翔，長峰萌華，末広志織，池谷鉄兵，鈴木隆史，山本雅之，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] Interaction of N-and C-terminal SH3 domains of Drosophila adapter protein Drk with Sos and Dos (2023)
  • Author(s): Sayeesh PM，Mayuki Iguchi，Haruka Sugasawa，Kahsuke Inomata，Teppei Ikeya，Yutaka Ito
  • Organizer: The 19th European Magnetic Resonance Congress
  • Int'l Joint Research
• [Presentation] In-cell NMRを用いたlinear diubiquitinの細胞内での立体構造解析 (2023)
  • Author(s): 屋部祥大，田岸亮馬，鴨志田一，美川 務，猪股晃介，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] 異種核多次元NMRによる根粒菌マルチドメイン蛋白質FixJの立体構造解析 (2023)
  • Author(s): 大久保里佳，堀川皓央，伊藤かおり，菱倉直樹，渡邉吏輝，三島正規，猪股晃介，小手石泰康，澤井仁美，城 宣嗣，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] Structural and dynamic studies on the Drosophila adapter Protein Drk” NMR討論会，横須賀，2023年11月7日 (2023)
  • Author(s): Sayeesh PM，Mayumi Iguchi，Teppei Ikeya，Yutaka Ito
  • Organizer: 第62回NMR討論会
• [Presentation] ストレス応答型の転写制御に関与するKeap1-Nrf2複合体のIn-cell NMR解析 (2023)
  • Author(s): 豊田芽生，猪股晃介，末広志織，島 海翔，長峰萌華，池谷鉄兵，鈴木隆史，山本雅之，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] 溶液NMRによるKRASとRGL2の相互作用の解析 (2023)
  • Author(s): 助川咲良，富樫直之，P.M. Sayeesh，菅澤はるか，猪股晃介，田仲加代子，伊藤 隆，池谷鉄兵
  • Organizer: 第62回NMR討論会
• [Presentation] NMRピークの線形解析を用いたKRAS/RGL2RBD滴定データの定量化 (2023)
  • Author(s): 富樫直之，助川咲良，菅澤はるか，美川 務，猪股晃介，田仲加代子，伊藤 隆，池谷鉄兵
  • Organizer: 第62回NMR討論会
• [Presentation] Drk蛋白質のN末端およびC末端SH3ドメインとSos/Dos由来ペプチドとの相互作用解析 (2023)
  • Author(s): 井口真由美，P.M. Sayeesh，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] 溶液NMRによるUbiquitin C-terminal Hydrolase L3 (UCHL3)の構造・ダイナミクス解析 (2023)
  • Author(s): 宮田裕貴，菅澤はるか，猪股晃介，伊藤 隆，池谷鉄兵
  • Organizer: 第62回NMR討論会
• [Presentation] GRB2とSOS1による多価相互作用の溶液NMR解析 (2023)
  • Author(s): 林 俊文，館野桂太，菅澤はるか，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] 蛋白質の立体構造に対する分子クラウディングの影響の解析 (2023)
  • Author(s): 加藤聖人，Sayeesh PM，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] NMRによるFixL-FixJ二成分シグナル伝達系の機能解析 (2023)
  • Author(s): 菱倉直樹，渡邉吏輝，大久保里佳，堀川皓央，伊藤かおり，三島正規，猪股晃介，小手石泰康，澤井仁美，城 宣嗣，池谷鉄兵，伊藤 隆
  • Organizer: 第62回NMR討論会
• [Presentation] Protein structure determination by NMR: conformational multiplicity and the effects of macromolecular crowding (2022)
  • Author(s): Yutaka Ito
  • Organizer: The 7th International Symposium on Drug Discovery and Design by NMR
  • Int'l Joint Research / Invited
• [Presentation] Solution NMR approaches to 3D structure determination of proteins in living cells (2022)
  • Author(s): Yutaka Ito
  • Organizer: The Protein Society Webinar “Exploring Proteins in Living Cells”
  • Int'l Joint Research / Invited
• [Presentation] 常磁性ランタノイド金属を用いた蛋白質の溶液NMRおよびin-cell NMR解析 (2022)
  • Author(s): 伊藤 隆
  • Organizer: 第1回生命金属科学シンポジウム
  • Invited
• [Presentation] Protein behaviours under intracellular crowding environments (2022)
  • Author(s): Yutaka Ito
  • Organizer: TMU Workshop on Protein NMR
  • Invited
• [Presentation] シアノバクテリアの光センサーにおける脱プロトン化した塩基性アミノ酸の観測 (2022)
  • Author(s): 永江 峰幸，飯塚 佑介，青山 洋史，宮ノ入 洋平，神野 智司，伊藤 隆，広瀬 侑，三島 正規
  • Organizer: 第61回NMR討論会
• [Presentation] NMR解析を用いた解糖系酵素PGKの環境適応的活性制御による解糖系調節機構 (2022)
  • Author(s): 八木 宏昌，葛西 卓磨，Elisa Rioual，池谷 鉄兵，木川 隆則
  • Organizer: 第61回NMR討論会
• [Presentation] NMR studies of a Drosophila adapter protein, Drk (2022)
  • Author(s): Sayeesh, PM, Teppei Ikeya, Haruka Sugasawa, Riki Watanabe, Yutaka Ito
  • Organizer: 第1回生命金属科学シンポジウム
• [Presentation] In-cell NMRによるlinear diubiquitinのin-situ立体構造解析 (2022)
  • Author(s): 屋部 祥大，田岸 亮馬，鴨志田 一，美川 務，猪股 晃介，池谷 鉄兵，伊藤 隆
  • Organizer: 第61回NMR討論会
• [Presentation] In-cell NMR によるKeap1/Nrf2系の研究 (2022)
  • Author(s): 豊田 芽生，猪股 晃介，末広 志織，島 海翔，池谷 鉄兵，鈴木 隆史，山本 雅之，伊藤 隆
  • Organizer: 第61回NMR討論会
• [Presentation] 常磁性 NMR を用いた根粒菌マルチドメイン蛋白質FixJの立体構造解析 (2022)
  • Author(s): 大久保 里佳，池谷 鉄兵，渡邉 吏輝，菱倉 直樹，三島 正規，猪股 晃介，小手石 泰康，澤井 仁美，城 宣嗣，伊藤 隆
  • Organizer: 第61回NMR討論会
• [Presentation] マルチドメイン蛋白質GRB2とSOS1-PRM領域の相互作用解析 (2022)
  • Author(s): 館野 桂太，菅澤 はるか，安藤 考史，田端 真彩子，美川 務，猪股 晃介，甲斐荘 正恒，三島 正規，杉田 有治，池谷 鉄兵，伊藤 隆
  • Organizer: 第61回NMR討論会
• [Presentation] 常磁性NMRを用いたマルチドメイン蛋白質GRB2の立体構造解析 (2022)
  • Author(s): 安藤 考史，菅澤 はるか，館野 桂太，田端 真彩子，美川 務，宮野入 洋平，川端 庸平，Hisham Dokainish，Weitong Ren，大出 真央，寺内 勉，猪股 晃介，三島 正規，甲斐荘 正恒，杉田 有治，池谷 鉄兵，伊藤 隆
  • Organizer: 第61回NMR討論会
• [Presentation] GMPPNP結合型K-RasG12VとRgl2RBDの相互作用解析 (2022)
  • Author(s): 富樫 直之，宮田 裕貴，亀井 駿，菅澤 はるか，美川 務，猪股 晃介，田仲 加代子，伊藤 隆，池谷 鉄兵
  • Organizer: 第61回NMR討論会
• [Presentation] Solution NMR approaches to investigating protein behaviours under intracellular crowding environments (2022)
  • Author(s): Yutaka Ito
  • Organizer: 29th International Conference on Magnetic Resonance in Biological Systems
  • Int'l Joint Research / Invited
• [Presentation] Solution NMR approaches to 3D structure determination of proteins in living eukaryotic cells (2021)
  • Author(s): Yutaka Ito, Kohsuke Inomata, Teppei Ikeya
  • Organizer: 22nd International Society of Magnetic Resonance Conference (ISMAR)
  • Int'l Joint Research / Invited
• [Presentation] 複数の異なる NMR データの統合解析によるタンパク質 multi-state 立体構造解析 (2021)
  • Author(s): 池谷 鉄兵，伊藤 隆
  • Organizer: 第21回日本蛋白質科学会年会
  • Invited
• [Presentation] Multi-state structure determination and dynamics analysis reveals a new ubiquitin-recognition mechanism in yeast ubiquitin C-terminal hydrolase (2021)
  • Author(s): Teppei Ikeya, Mayu Okada, Yutaka Tateishi, Eri Nojiri, Tsutomu Mikawa, Sundaresan Rajesh, Hiroki Ogasa, Takumi Ueda, Hiromasa Yagi, Toshiyuki Kohno, Takanori Kigawa, Ichio Shimada, Peter G?ntert, Yutaka Ito
  • Organizer: 22nd International Society of Magnetic Resonance Conference (ISMAR)
  • Int'l Joint Research
• [Presentation] Insight into the C-terminal SH3 mediated binding of Drosophila Drk towards Sos and Dos (2021)
  • Author(s): Sayeesh. P. M., Teppei Ikeya, Haruka Sugasawa, Riki Watanabe, Yutaka Ito
  • Organizer: 22nd International Society of Magnetic Resonance Conference (ISMAR)
  • Int'l Joint Research
• [Presentation] Insight into the C-terminal SH3 mediated binding of Drosophila Drk towards Sos and Dos (2021)
  • Author(s): Sayeesh. P. M., Teppei Ikeya, Haruka Sugasawa, Riki Watanabe, Yutaka Ito
  • Organizer: 第44回日本分子生物学会
• [Presentation] NMRによるKRASとRGL2の相互作用解析 (2021)
  • Author(s): 富樫直之，亀井駿，菅澤はるか，猪股晃介，伊藤隆，田仲加代子，池谷鉄兵
  • Organizer: 第44回日本分子生物学会
• [Presentation] 直鎖状ユビキチン二量体の溶液構造解析 (2021)
  • Author(s): 屋部 祥大，田岸亮馬，鴨志田一，美川務，猪股晃介，池谷鉄兵，伊藤隆
  • Organizer: 第44回日本分子生物学会
• [Presentation] In-cell NMRを用いたKeap1-Nrf2制御系の解析 (2021)
  • Author(s): 末広志織，猪股晃介，豊田芽生，池谷鉄兵，鈴木隆史，山本 雅之，伊藤隆
  • Organizer: 第44回日本分子生物学会
• [Presentation] Solution NMR approaches to investigating protein behaviours under intracellular crowding environments (2021)
  • Author(s): Yutaka Ito, Teppei Ikeya
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2020
  • Int'l Joint Research / Invited
• [Presentation] Solution NMR Approaches to Investigating Protein Behaviours under Intracellular Crowding Environments (2021)
  • Author(s): Yutaka Ito, Kohsuke Inomata, Teppei Ikeya
  • Organizer: 22nd International Society of Magnetic Resonance Conference, the 9th Asia-Pacific NMR Symposium
  • Int'l Joint Research / Invited
• [Presentation] 複数の異なるNMR データの統合解析によるタンパク質multi-state 立体構造解析 (2020)
  • Author(s): 池谷 鉄兵，伊藤 隆
  • Organizer: 第58回日本生物物理学会
  • Invited
• [Presentation] Solution NMR approaches to 3D structure determination of proteins in living eukaryotic cells (2019)
  • Author(s): Ito Yutaka, Ikeya Teppei
  • Organizer: 33rd Annual Symposium of the Protein Society, Seattle, USA
  • Int'l Joint Research / Invited
• [Presentation] High resolution protein 3D structure determination in living eukaryotic cells (2019)
  • Author(s): Ito Yutaka, Ikeya Teppei
  • Organizer: The 8th Asia Pacific NMR Symposium, Singapore
  • Int'l Joint Research / Invited
• [Presentation] High-resolution protein 3D structure determination in living eukaryotic cells (2019)
  • Author(s): Tanaka Takashi, Ikeya Teppei, Mishima Masaki, Ito Yutaka
  • Organizer: The 12th Australian and New Zealand Society for Magnetic Resonance Conference (ANZMAG conference 2019), Cape Naturaliste, Australia
  • Int'l Joint Research / Invited
• [Presentation] 溶液NMRを用いたin situ構造生物学 (2019)
  • Author(s): 伊藤 隆，池谷鉄兵
  • Organizer: 第13回バイオ関連化学シンポジウム2019
  • Invited
• [Presentation] Solution NMR approaches to 3D structure determination of proteins in living eukaryotic cells (2019)
  • Author(s): 伊藤 隆，池谷鉄兵
  • Organizer: 第42回日本分子生物学会年会 シンポジウム「In-cell protein scienceのフロンティア」
  • Invited
• [Book] Integrated Structural Biology - Chapter 5: In-cell Structural Biology Through the Integration of Solution NMR Spectroscopy and Computational Science (2023)
  • Author(s): Y. Ito, T. Ikeya, K. Inomata
  • Total Pages: 404
  • Publisher: Royal Society of Chemistry
  • ISBN: 9781839161834
• [Book] 生命金属ダイナミクス―生体内における金属の挙動と制御 (2021)
  • Author(s): 津本浩平，城宜嗣，他
  • Total Pages: 511
  • Publisher: エヌ・ティー・エス
  • ISBN: 4860437063
• [Book] In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology (2019)
  • Author(s): Ito Yutaka, Doetsch Volker, Shirakawa Masahiro
  • Total Pages: 305
  • Publisher: Royal Society of Chemistry
  • ISBN: 9781788012171