| Budget Amount *help |
¥78,130,000 (Direct Cost: ¥60,100,000、Indirect Cost: ¥18,030,000)
Fiscal Year 2023: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2022: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2021: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
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| Outline of Final Research Achievements |
We have studied on (1) development of in-cell NMR methods, (2) analysis of biometal-related proteins using solution and in-cell NMR spectroscopy, and (3) potential applications of in-cell NMR.
In (1), we developed an in-cell NMR method using cultured plant cells and a method for 3D structural analysis of multi-domain proteins using paramagnetic NMR information. In (2), we analysed the rhizobial two-component signal transduction system FixJ/FixL system. For FixJ, we determined the detailed solution structures as well as analysing the interaction with FixL and the effect of phosphorylation. In (3), results were obtained for in-cell NMR analysis of unstable proteins in human cultured cells.
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