| Budget Amount *help |
¥134,290,000 (Direct Cost: ¥103,300,000、Indirect Cost: ¥30,990,000)
Fiscal Year 2023: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2022: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2021: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2020: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2019: ¥35,490,000 (Direct Cost: ¥27,300,000、Indirect Cost: ¥8,190,000)
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| Outline of Final Research Achievements |
Research was conducted on various targets to visualize the reaction processes of enzymes and proteins as molecular movies. From the molecular dynamics simulations and the substrate-free crystal structures of Dieals-Alderases, Phm7 and Fsa2, we proposed a substrate binding mode that can explain the stereoselective reaction mechanism of the enzymes, and confirmed its accuracy with crystal structures of the methylated product-bound form of Fsa2. The order and position of the Ca2+ binding that induces the luminescence of Aequorin were also clarified. Furthermore, we determined the structure of the active and ligand-free form of Rubisco and showed that this structure is distinct from both the open and closed forms of the enzymes known so far.
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