Reaction initiation techniques in time-resolved experiments

• Project Area: Non-equilibrium-state molecular movies and their applications
• Project/Area Number: 19H05781
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tohoku University (2020-2023); Institute of Physical and Chemical Research (2019)
• Principal Investigator: Nango Eriko 東北大学, 多元物質科学研究所, 教授 (90376947)
• Co-Investigator(Kenkyū-buntansha): 清水 伸隆 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (20450934) ; 大和田 成起 公益財団法人高輝度光科学研究センター, XFEL利用研究推進室, 主幹研究員 (90725962) ; 宮下 治 国立研究開発法人理化学研究所, 計算科学研究センター, 上級研究員 (10620528) ; 庄司 光男 筑波大学, 計算科学研究センター, 助教 (00593550) ; 篠田 恵子 東京大学, 先端科学技術研究センター, 特任助教 (80646951)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥203,060,000 (Direct Cost: ¥156,200,000、Indirect Cost: ¥46,860,000); Fiscal Year 2023: ¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000); Fiscal Year 2022: ¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000); Fiscal Year 2021: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000); Fiscal Year 2020: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000); Fiscal Year 2019: ¥38,610,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥8,910,000)
• Keywords: X線自由電子レーザー / 時分割実験 / 溶液散乱 / 温度ジャンプ / 結晶構造解析 / 動的構造 / X線結晶構造解析 / X線溶液散乱 / 蛋白質結晶構造解析 / 動的構造解析 / シリアルフェムト秒結晶構造解析 / タンパク質ダイナミクス / 構造モデリング

Outline of Research at the Start

X 線自由電子レーザー（XFEL）を用いたタンパク質構造解析法は、タンパク質が起こす素早い反応・構造変化を原子レベルまで捉えられる新たな手法として、国内外で非常に期待されている。本研究計画では、XFELによって多種多様なタンパク質の構造変化や反応過程を捉えるための技術開発を行う。本技術により、生体分子の機能やその機構に対する理解が飛躍的に深まると共に、構造情報に基づく生体分子制御の応用に貢献することが期待される。

Outline of Final Research Achievements

Time-resolved serial femtosecond crystallography (SFX) using an X-ray free electron laser is currently the only method that can obtain protein dynamic structures with high temporal and spatial resolution. In particular, time-resolved SFX using a pump laser has led to successful results. However, most of proteins are photo non-excitable, and it is necessary to develop a versatile reaction initiation technique. In this project, we worked on the development of a temperature jump method for SFX and "mixed and inject" SFX experiments by rapid mixing of substrates. We also developed a solution scattering method using an X-ray free electron laser to overcome the limitations imposed by crystals.

Academic Significance and Societal Importance of the Research Achievements

時分割SFX はその高い空間・時間分解能から、これまで原子分解能で追うことのできなかった化学反応などの早い反応の追跡に適している。従って、従来得られなかった構造的及び化学的な知見を得られることが期待される。本班はこうした動的な構造変化を可視化するための技術の確立を目指すだけでなく、本領域のA01から提供される数多くのタンパク質試料の動的構造解析の促進に貢献できる。また、ケミカルバイオロジーや計算科学の班と連携することにより、本法を更に発展させることが期待される。

Research Products

• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of California, Merced(米国)
• [Int'l Joint Research] 台湾中央研究院/台湾大学(台湾)
• [Int'l Joint Research] University of Cambridge(英国)
• [Int'l Joint Research] University of Strasbourg(フランス)
• [Journal Article] Crystal structure reveals the binding mode and selectivity of a photoswitchable ligand for the adenosine A2A receptor (2024)
  • Author(s): Araya Tsuyoshi、Matsuba Yuya、Suzuki Harufumi、Doura Tomohiro、Nuemket Nipawan、Nango Eriko、Yamamoto Masaki、Im Dohyun、Asada Hidetsugu、Kiyonaka Shigeki、Iwata So
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 695 Pages: 149393-149393
  • DOI: 10.1016/j.bbrc.2023.149393
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oxygen-evolving photosystem II structures during S1-S2-S3 transitions (2024)
  • Author(s): Li Hongjie、Nakajima Yoshiki、Nango Eriko、Owada Shigeki、Yamada Daichi、Tosha Takehiko、Tono Kensuke、Joti Yasumasa、Hatsui Takaki、Yabashi Makina、Kubo Minoru、Iwata So、Isobe Hiroshi、Yamaguchi Kizashi、Suga Michihiro、Shen Jian-Ren
  • Journal Title: Nature Volume: 626 Issue: 7999 Pages: 670-677
  • DOI: 10.1038/s41586-023-06987-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Understanding the effects of ethanol on the liposome bilayer structure using microfluidic-based time-resolved small-angle X-ray scattering and molecular dynamics simulations (2024)
  • Author(s): Maeki Masatoshi、Kimura Niko、Okada Yuto、Shimizu Kazuki、Shibata Kana、Miyazaki Yusuke、Ishida Akihiko、Yonezawa Kento、Shimizu Nobutaka、Shinoda Wataru、Tokeshi Manabu
  • Journal Title: Nanoscale Advances Volume: ー Issue: 8 Pages: 2166-2176
  • DOI: 10.1039/d3na01073b
  • Peer Reviewed / Open Access
• [Journal Article] Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport (2024)
  • Author(s): Watanabe Satoshi、Kise Yoshiaki、Yonezawa Kento、Inoue Mariko、Shimizu Nobutaka、Nureki Osamu、Inaba Kenji
  • Journal Title: Nature Communications Volume: 15 Issue: 1 Pages: 2404-2404
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• [Journal Article] Heme-substituted protein assembly bridged by synthetic porphyrin: achieving controlled configuration while maintaining rotational freedom (2024)
  • Author(s): Inaba Hiroaki、Shisaka Yuma、Ariyasu Shinya、Sakakibara Erika、Ueda Garyo、Aiba Yuichiro、Shimizu Nobutaka、Sugimoto Hiroshi、Shoji Osami
  • Journal Title: RSC Advances Volume: 14 Issue: 13 Pages: 8829-8836
  • DOI: 10.1039/d4ra01042f
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• [Journal Article] SAXS/WAXS data of conformationally flexible ribose binding protein (2024)
  • Author(s): Choudhury Jagrity、Yonezawa Kento、Anu Anu、Shimizu Nobutaka、Chaudhuri Barnali
  • Journal Title: Data in Brief Volume: 52 Pages: 109932-109932
  • DOI: 10.1016/j.dib.2023.109932
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• [Journal Article] Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase (2023)
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  • Journal Title: Science Advances Volume: 9 Issue: 49 Pages: 1-12
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  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Visualizing the DNA repair process by a photolyase at atomic resolution (2023)
  • Author(s): Manuel Maestre-Reyna, Po-Hsun Wang, Eriko Nango, Yuhei Hosokawa, Martin Saft , Antonia Furrer , Cheng-Han Yang , Eka Putra Gusti Ngurah Putu , Wen-Jin Wu, (…), So Iwata ,(…..) and Ming-Daw Tsai
  • Journal Title: Science (New York, N.Y.) Volume: Vol.382, No.6674 Issue: 6674
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  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 多様化するバイオテクノロジーと光技術 X線自由電子レーザーで捉えたビフィズス菌酵素の常温構造 (2023)
  • Author(s): 中田國夫, 柏木立己, 南後恵理子, 岩田想
  • Journal Title: 光アライアンス (Optical Alliance) Volume: 34巻10号 Pages: 1-4
• [Journal Article] A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography (2023)
  • Author(s): Birch James、Kwan Tristan O. C.、Judge Peter J.、Axford Danny、Aller Pierre、Butryn Agata、Reis Rosana I.、Bada Juarez Juan F.、Vinals Javier、Owen Robin L.、Nango Eriko、Tanaka Rie、Tono Kensuke、Joti Yasumasa、Tanaka Tomoyuki、Owada Shigeki、Sugahara Michihiro、Iwata So、Orville Allen M.、Watts Anthony、Moraes Isabel
  • Journal Title: Journal of Applied Crystallography Volume: 56 Issue: 5 Pages: 1361-1370
  • DOI: 10.1107/s1600576723006428
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography (2023)
  • Author(s): Wolff, A. M., Nango, E., Young, I. D., Brewster, A. S., Kubo, M., Nomura, T., Sugahara, M., Owada, S., Barad, B. A., Ito, K., Bhowmick, A., Carbajo, S., Hino, T., Holton, J. M., Im, D., O'Riordan, L. J., Tanaka, T., Tanaka, R., Sierra, R. G., Yumoto, F., Tono, K., Iwata, S., et al
  • Journal Title: Nat. Chem. Volume: 15(11) Issue: 11 Pages: 1549-1558
  • DOI: 10.1038/s41557-023-01329-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Optical control of ultrafast structural dynamics in a fluorescent protein (2023)
  • Author(s): Hutchison Christopher D. M. et al.
  • Journal Title: Nature Chemistry Volume: 15 Issue: 11 Pages: 1607-1615
  • DOI: 10.1038/s41557-023-01275-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent progress in membrane protein dynamics revealed by X-ray free electron lasers: Molecular movies of microbial rhodopsins (2023)
  • Author(s): Eriko Nango and So Iwata
  • Journal Title: Curr. Opin. Struct.Biol. Volume: 81 Pages: 102629-102629
  • DOI: 10.1016/j.sbi.2023.102629
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism (2023)
  • Author(s): Fadini Alisia et al.
  • Journal Title: Journal of the American Chemical Society Volume: 145 Issue: 29 Pages: 15796-15808
  • DOI: 10.1021/jacs.3c02313
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and characterization of endo-α-, exo-α-, and exo-β-d-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria (2023)
  • Author(s): Shimokawa Michiko、Ishiwata Akihiro、Kashima Toma、et al.
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 5803-5803
  • DOI: 10.1038/s41467-023-41431-2
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• [Journal Article] The two‐step cargo recognition mechanism of heterotrimeric kinesin (2023)
  • Author(s): Jiang Xuguang、Ogawa Tadayuki、Yonezawa Kento、Shimizu Nobutaka、Ichinose Sotaro、Uchihashi Takayuki、Nagaike Wataru、Moriya Toshio、Adachi Naruhiko、Kawasaki Masato、Dohmae Naoshi、Senda Toshiya、Hirokawa Nobutaka
  • Journal Title: EMBO reports Volume: 24 Issue: 11
  • DOI: 10.15252/embr.202356864
  • Peer Reviewed / Open Access
• [Journal Article] MOLASS: Software for automatic processing of matrix data obtained from small-angle X-ray scattering and UV–visible spectroscopy combined with size-exclusion chromatography (2023)
  • Author(s): Yonezawa Kento、Takahashi Masatsuyo、Yatabe Keiko、Nagatani Yasuko、Shimizu Nobutaka
  • Journal Title: Biophysics and Physicobiology Volume: 20 Issue: 1 Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v20.0001
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers. (2022)
  • Author(s): Hosaka, T. et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 119 Issue: 9
  • DOI: 10.1073/pnas.2117433119
  • Peer Reviewed / Open Access
• [Journal Article] Serial femtosecond X-ray crystallography of an anaerobically formed catalytic intermediate of copper amine oxidase (2022)
  • Author(s): Murakawa Takeshi、Suzuki Mamoru、Fukui Kenji、Masuda Tetsuya、Sugahara Michihiro、Tono Kensuke、Tanaka Tomoyuki、Iwata So、Nango Eriko、Yano Takato、Tanizawa Katsuyuki、Okajima Toshihide
  • Journal Title: Acta Crystallographica Section D Structural Biology Volume: 78 Issue: 12 Pages: 1428-1438
  • DOI: 10.1107/s2059798322010385
  • Peer Reviewed / Open Access
• [Journal Article] Ca ²⁺ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha (2022)
  • Author(s): Takahashi Daisuke、Yonezawa Kento、Okizaki Yuki、Caaveiro Jose M. M.、Ueda Tadashi、Shimada Atsushi、Sakane Fumio、Shimizu Nobutaka
  • Journal Title: Protein Science Volume: 31 Issue: 7
  • DOI: 10.1002/pro.4365
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX (2021)
  • Author(s): Pan Dongqing、Oyama Ryo、Sato Tomomi、Nakane Takanori、Mizunuma Ryo、Matsuoka Keita、Joti Yasumasa、Tono Kensuke、Nango Eriko、Iwata So、Nakatsu Toru、Kato Hiroaki
  • Journal Title: IUCrJ Volume: 9 Issue: 1 Pages: 134-145
  • DOI: 10.1107/s2052252521011611
  • NAID: 120007179308
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase (2021)
  • Author(s): Murakawa Takeshi、Suzuki Mamoru、Arima Toshi、Sugahara Michihiro、Tanaka Tomoyuki、Tanaka Rie、Iwata So、Nango Eriko、Tono Kensuke、Hayashi Hideyuki、Fukui Kenji、Yano Takato、Tanizawa Katsuyuki、Okajima Toshihide
  • Journal Title: Acta Crystallographica Section F Structural Biology Communications Volume: 77 Issue: 10 Pages: 356-363
  • DOI: 10.1107/s2053230x21008967
  • Peer Reviewed
• [Journal Article] High-resolution crystal structures of transient intermediates in the phytochrome photocycle (2021)
  • Author(s): Carrillo Melissa et al.
  • Journal Title: Structure Volume: 未定 Issue: 7 Pages: 743-754
  • DOI: 10.1016/j.str.2021.03.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Capturing structural changes of the S1 to S2 transition of photosystem II using time-resolved serial femtosecond crystallography (2021)
  • Author(s): H Li, Y Nakajima, T Nomura, M Sugahara, S Yonekura, SK Chan, T Nakane, T Yamane, Y Umena, M Suzuki, T Masuda, T Motomura, H Naitow, Y Matsuura, T Kimura, K Tono, S Owada, Y Joti, R Tanaka, E Nango, F Akita, M Kubo, S Iwata, JR Shen and M Suga
  • Journal Title: IUCrJ Volume: 8 Issue: 3 Pages: 431-443
  • DOI: 10.1107/s2052252521002177
  • NAID: 120007037379
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure of the dopamine D2 receptor in complex with the antipsychotic drug spiperone (2020)
  • Author(s): Im Dohyun、Inoue Asuka、Fujiwara Takaaki、Nakane Takanori、Yamanaka Yasuaki、Uemura Tomoko、Mori Chihiro、Shiimura Yuki、Kimura Kanako Terakado、Asada Hidetsugu、Nomura Norimichi、Tanaka Tomoyuki、Yamashita Ayumi、Nango Eriko、Tono Kensuke、Kadji Francois Marie Ngako、Aoki Junken、Iwata So、Shimamura Tatsuro
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41467-020-20221-0
  • NAID: 120006943309
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isoprenoid-chained lipid EROCOC17+4: a new matrix for membrane protein crystallization and a crystal delivery medium in serial femtosecond crystallography (2020)
  • Author(s): Ihara Kentaro、Hato Masakatsu、Nakane Takanori、Yamashita Keitaro、Kimura-Someya Tomomi、Hosaka Toshiaki、Ishizuka-Katsura Yoshiko、Tanaka Rie、Tanaka Tomoyuki、Sugahara Michihiro、Hirata Kunio、Yamamoto Masaki、Nureki Osamu、Tono Kensuke、Nango Eriko、Iwata So、Shirouzu Mikako
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 19305-19305
  • DOI: 10.1038/s41598-020-76277-x
  • Peer Reviewed / Open Access
• [Journal Article] A tool for visualizing protein motions in time-resolved crystallography (2020)
  • Author(s): Wickstrand Cecilia、Katona Gergely、Nakane Takanori、Nogly Przemyslaw、Standfuss Joerg、Nango Eriko、Neutze Richard
  • Journal Title: Structural Dynamics Volume: 7 Issue: 2 Pages: 024701-024701
  • DOI: 10.1063/1.5126921
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• [Journal Article] The Measles Virus V Protein Binding Site to STAT2 Overlaps That of IRF9 (2020)
  • Author(s): Nagano Yuma、Sugiyama Aoi、Kimoto Madoka、Wakahara Takuya、Noguchi Yasuyo、Jiang Xinxin、Saijo Shinya、Shimizu Nobutaka、Yabuno Nana、Yao Min、Gooley Paul R.、Moseley Gregory W.、Tadokoro Takashi、Maenaka Katsumi、Ose Toyoyuki
  • Journal Title: Journal of Virology Volume: 94 Issue: 17
  • DOI: 10.1128/jvi.01169-20
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• [Journal Article] Crystal structure of GCN5 PCAF N-terminal domain reveals atypical ubiquitin ligase structure (2020)
  • Author(s): Toma-Fukai Sachiko、Hibi Ryota、Naganuma Takao、Sakai Mashito、Saijo Shinya、Shimizu Nobutaka、Matsumoto Michihiro、Shimizu Toshiyuki
  • Journal Title: Journal of Biological Chemistry Volume: 295 Issue: 43 Pages: 14630-14639
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• [Journal Article] Integrative/Hybrid Modeling Approaches for Studying Biomolecules (2020)
  • Author(s): Srivastava Ashutosh、Tiwari Sandhya Premnath、Miyashita Osamu、Tama Florence
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  • DOI: 10.1016/j.jmb.2020.01.039
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• [Journal Article] Reaction Mechanism of Monomeric Sarcosine Oxidase with N-Cyclopropylglycine (2020)
  • Author(s): M.Shoji, Y.Abe, M.Boero, Y.Shigeta, Y.Nishiya
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  • DOI: 10.1039/d0cp01679a
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• [Journal Article] A New Lipid Force Field (FUJI) (2020)
  • Author(s): Kamiya Nozomu、Kayanuma Megumi、Fujitani Hideaki、Shinoda Keiko
  • Journal Title: Journal of Chemical Theory and Computation Volume: - Issue: 6 Pages: 3664-3676
  • DOI: 10.1021/acs.jctc.9b01195
  • Peer Reviewed
• [Journal Article] Optimal Mutant Model of Human S100A3 Protein Citrullinated at Arg51 by Peptidylarginine Deiminase Type III and Its Solution Structural Properties (2020)
  • Author(s): Ite, K., Yonezawa, K., Kitanishi, K., Shimizu, N., Unno, M.
  • Journal Title: ACS Omega Volume: 5 Issue: 8 Pages: 4032-4042
  • DOI: 10.1021/acsomega.9b03618
  • Peer Reviewed / Open Access
• [Journal Article] Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins (2019)
  • Author(s): Miyamoto Takaaki、Hayashi Yugo、Yoshida Keito、Watanabe Hiroki、Uchihashi Takayuki、Yonezawa Kento、Shimizu Nobutaka、Kamikubo Hironari、Hirota Shun
  • Journal Title: ACS Synthetic Biology Volume: 印刷中 Issue: 5 Pages: 1112-1120
  • DOI: 10.1021/acssynbio.9b00019
  • NAID: 120006801961
  • Peer Reviewed / Open Access
• [Journal Article] Structure of tRNA methyltransferase complex of Trm7 and Trm734 reveals a novel binding interface for tRNA recognition (2019)
  • Author(s): Hirata Akira、Okada Keisuke、Yoshii Kazuaki、Shiraishi Hiroyuki、Saijo Shinya、Yonezawa Kento、Shimizu Nobutaka、Hori Hiroyuki
  • Journal Title: Nucleic Acids Research Volume: 47 Issue: 20 Pages: 10942-10955
  • DOI: 10.1093/nar/gkz856
  • Peer Reviewed / Open Access
• [Journal Article] Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers (2019)
  • Author(s): James Daniel、Weinert Tobias、Skopintsev Petr、Furrer Antonia、Gashi Dardan、Tanaka Tomoyuki、Nango Eriko、Nogly Przemyslaw、Standfuss Joerg
  • Journal Title: Journal of Visualized Experiments Volume: - Issue: 144
  • DOI: 10.3791/59087
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure (2019)
  • Author(s): Shimazu Yoshiaki、Tono Kensuke、Tanaka Tomoyuki、Yamanaka Yasuaki、Nakane Takanori、Mori Chihiro、Terakado Kimura Kanako、Fujiwara Takaaki、Sugahara Michihiro、Tanaka Rie、Doak R. Bruce、Shimamura Tatsuro、Iwata So、Nango Eriko、Yabashi Makina
  • Journal Title: Journal of Applied Crystallography Volume: 52 Issue: 6 Pages: 1280-1288
  • DOI: 10.1107/s1600576719012846
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies (2019)
  • Author(s): Nango Eriko、Kubo Minoru、Tono Kensuke、Iwata So
  • Journal Title: Applied Sciences Volume: 9 Issue: 24 Pages: 5505-5505
  • DOI: 10.3390/app9245505
  • Peer Reviewed / Open Access
• [Presentation] X線自由電子レーザーによる時分割実験の現状と展望 (2024)
  • Author(s): 南後 恵理子
  • Organizer: 日本農芸化学会大会
  • Invited
• [Presentation] タンパク質動的解析による反応機構解明 (2024)
  • Author(s): 南後 恵理子
  • Organizer: 日本化学会第104春季年会
  • Invited
• [Presentation] Measurement systems for biomolecular movies using X-ray free electron lasers (2024)
  • Author(s): 南後 恵理子
  • Organizer: SACLA User's Meeting 2023
  • Int'l Joint Research
• [Presentation] Photon FactoryのSAXSビームライン (2024)
  • Author(s): 清水 伸隆、高木秀彰、森丈晴、永谷康子、西條慎也、中村文俊、羽方望、菊池槙子、谷田部 景子、高橋 正剛、五十嵐教之
  • Organizer: 第37回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] 時分割実験のための多様な反応誘起システムの開発 (2023)
  • Author(s): 南後 恵理子
  • Organizer: 令和５年度 新学術領域「高速分子動画」領域会議
• [Presentation] 室温下のX線結晶構造解析が見せる新たな可能性 (2023)
  • Author(s): 南後 恵理子
  • Organizer: 日本結晶学会オンライン講演会第１回CrSJ Keynotes
  • Invited
• [Presentation] Seeing is believing:X線自由電子レーザーによるタンパク質分子動画解析 (2023)
  • Author(s): 南後 恵理子
  • Organizer: 生物物理学会若手の会夏の学校
• [Presentation] 3D movies of structural changes in proteins captured by X-ray free electron lasers (2023)
  • Author(s): 南後 恵理子
  • Organizer: the 9th CWRU x Tohoku Joint Workshop
  • Int'l Joint Research / Invited
• [Presentation] X線自由電子レーザーによるタンパク質分子動画解析 (2023)
  • Author(s): 南後 恵理子
  • Organizer: 第 17 回 日本分子イメージング学会 総会・学術集会
  • Invited
• [Presentation] 時分割実験のための多様な反応誘起システムの開発 (2023)
  • Author(s): 南後 恵理子
  • Organizer: 令和5年度「高速分子動画」シンポジウム・領域会議（横浜）
  • Invited
• [Presentation] Time-resolved serial femtosecond crystallography of microbial rhodopsins (2023)
  • Author(s): 南後 恵理子
  • Organizer: EMBO Workshop Time-resolved spectroscopy meets time-resolved crystallography: The future of dynamic photobiology
  • Int'l Joint Research / Invited
• [Presentation] Photon FactoryにおけるBioSAXS測定解析システム (2023)
  • Author(s): 清水伸隆, 菊地槙子, 高橋正剛, 谷田部景子, 永谷康子
  • Organizer: 令和5年（2023年）度日本結晶学会年会
• [Presentation] Photon FactoryのSAXSビームライン (2023)
  • Author(s): 清水 伸隆、高木秀彰、森丈晴、永谷康子、西條慎也、中村文俊、羽方望、菊池槙子、谷田部 景子、高橋 正剛、五十嵐教之
  • Organizer: 第37回日本放射光学会年会・放射光科学合同シンポジウム
  • Int'l Joint Research
• [Presentation] Recent activities of biology research fields and requests for facilities (tentative) (2023)
  • Author(s): 南後恵理子
  • Organizer: SACLA User's Meeting 2023
  • Int'l Joint Research
• [Presentation] X線自由電子レーザーによるタンパク質分子動画解析 (2023)
  • Author(s): 南後恵理子
  • Organizer: 2022年度iBIX-JAXA-KEK物構研-QST合同タンパク質研究会
  • Invited
• [Presentation] XFELによるタンパク質分子動画解析の現状と今後の展望 (2023)
  • Author(s): 南後恵理子
  • Organizer: 第３６回日本放射光学会年会・放射光科学合同シンポジウム
  • Invited
• [Presentation] Photon FactoryのBioSAXS測定解析システムの現状 (2023)
  • Author(s): 清水 伸隆、谷田部 景子、高橋 正剛、永谷 康子
  • Organizer: 第36回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] X 線自由電子レーザーによるタンパク質分子動画解析 (2022)
  • Author(s): 南後恵理子
  • Organizer: 光・量子デバイス研究会
  • Invited
• [Presentation] 3D Movies of Structural Changes in Proteins Captured by X-ray Free Electron Lasers (2022)
  • Author(s): 南後恵理子
  • Organizer: AsBIC10(10th Asian Biological Inorganic Chemistry Conference)
  • Int'l Joint Research / Invited
• [Presentation] 高速分子動画計測の現状と今後の課題 (2022)
  • Author(s): 南後恵理子
  • Organizer: 新学術領域会議
  • Invited
• [Presentation] X 線自由電子レーザーによる高時空間分解能タンパク質分子動画 (2022)
  • Author(s): 南後恵理子
  • Organizer: 第８回電子状態理論シンポジウム
  • Invited
• [Presentation] 高速分子動画法で観るタンパク質構造変化 (2022)
  • Author(s): 南後恵理子
  • Organizer: 第95回日本生化学会大会
  • Invited
• [Presentation] Time-resolved serial femtosecond crystallography of microbial rhodopsins (2022)
  • Author(s): 南後恵理子
  • Organizer: ICRP2022
  • Int'l Joint Research / Invited
• [Presentation] Toward understanding of enzyme reaction mechanism: serial crystallography using mixing injectors (2022)
  • Author(s): 南後恵理子
  • Organizer: 2022 SSRL/LCLS USERS' MEETING
  • Int'l Joint Research / Invited
• [Presentation] 3D movies of structural changes in proteins captured by X-ray free electron lasers (2022)
  • Author(s): 南後恵理子
  • Organizer: Sendai2022 workshop, An Update on Molecular Machines: Open Challenges and New Perspectives
  • Int'l Joint Research / Invited
• [Presentation] 高速分子動画法で観るタンパク質構造変化 (2022)
  • Author(s): 南後恵理子
  • Organizer: Agtechセミナー
  • Invited
• [Presentation] X線自由電子レーザーを用いた膜タンパク質分子動画解析 (2022)
  • Author(s): 南後恵理子
  • Organizer: バイオインタストリー協会「発酵と代謝研究会」第１回勉強会
  • Invited
• [Presentation] 百聞は一見に如かず：タンパク質分子動画から見えてくる世界 (2022)
  • Author(s): 南後恵理子
  • Organizer: LINK-J×東北大学次世代放射光イベント
  • Invited
• [Presentation] Time-resolved serial femtosecond crystallography of microbial rhodopsins (2022)
  • Author(s): 南後恵理子
  • Organizer: “The Molecular Movies and beyond” International Symposium 2022
  • Int'l Joint Research / Invited
• [Presentation] 温度ジャンプ法による分子動画解析の技術開発 (2022)
  • Author(s): 南後恵理子
  • Organizer: 新学術領域「高速分子動画」国際シンポジウム・令和 4 年度領域会議
  • Invited
• [Presentation] Structural state estimation of biological macromolecules in solution using BioSAXS (2022)
  • Author(s): Nobutaka Shimizu
  • Organizer: 蛋白研セミナー：Frontier of Dynamic Structural Biology （招待講演）
  • Int'l Joint Research / Invited
• [Presentation] BioSAXS measurement and analysis system at the Photon Factory (2022)
  • Author(s): Nobutaka Shimizu
  • Organizer: Asia Oceania International Conference on Synchrotron Radiation Instruments 2022 (AO-SRI2022)
  • Int'l Joint Research
• [Presentation] Recent developments of experimental platforms to observe the ultrafast phenomena at SACLA (2022)
  • Author(s): 大和田成起
  • Organizer: International Symposium on Ultrafast Intense Laser Science 2022
  • Int'l Joint Research
• [Presentation] Tracking enzymatic reactions by mix;d-inject serial femtosecond crystallography (2021)
  • Author(s): 南後恵理子
  • Organizer: PACIFICHEM2021(The International Chemical Congress of Pacific Basin Societies 2021)
  • Int'l Joint Research / Invited
• [Presentation] X線自由電子レーザーによるタンパク質分子動画解析 (2021)
  • Author(s): 南後恵理子
  • Organizer: 日本化学会 東海コンファレンス2021
• [Presentation] 原子レベルの動的構造解析が拓く生体分子機能の理解 (2021)
  • Author(s): 南後恵理子
  • Organizer: 第59回日本生物物理学会年会(BSJ2021)
  • Invited
• [Presentation] 二液混合シリアルフェムト秒結晶構造解析による酵素反応追跡 (2021)
  • Author(s): 南後恵理子
  • Organizer: 日本結晶学会年会
• [Presentation] 時分割SFXにおける課題と展望 (2021)
  • Author(s): 南後恵理子
  • Organizer: 令和3年度 新学術領域「高速分子動画」シンポジウム
• [Presentation] SACLAでの時分割構造解析 (2021)
  • Author(s): 南後恵理子
  • Organizer: よこはまNMR研究会 第６７回ワークショップ「放射光解析の最前線」
  • Invited
• [Presentation] Protein structural;dynamics revealed by molecular movie analysis (2021)
  • Author(s): 南後恵理子
  • Organizer: 第３５回アメリカ蛋白質科学会
  • Int'l Joint Research / Invited
• [Presentation] 高速分子動画:タンパク質の構造機能相関研究の最先端 Non-equilibrium state molecular movies: a new frontier in structural and functional studies on proteins (2021)
  • Author(s): 南後恵理子
  • Organizer: 第２１回日本蛋白質科学会年会
  • Invited
• [Presentation] 高速分子動画が捉えるタンパク質構造ダイナミクスProtein structural dynamics captured by molecular movie analysis (2021)
  • Author(s): 南後恵理子
  • Organizer: 日本顕微鏡学会第７７回学術講演会
  • Invited
• [Presentation] X線自由電子レーザーによる分子動画解析 (2021)
  • Author(s): 南後恵理子
  • Organizer: 第47回生体分子科学討論会
  • Invited
• [Presentation] Time-resolved serial femtosecond crystallography of light-sensitive proteins (2021)
  • Author(s): Eriko Nango
  • Organizer: Belgian Crystallography Symposium BCS-11
  • Int'l Joint Research / Invited
• [Presentation] Measurement Systems for Biomolecular Movies using X-ray Free Electron Lasers (2021)
  • Author(s): Eriko Nango
  • Organizer: SACLA Users' Meeting 2021
  • Int'l Joint Research
• [Presentation] Protein structural dynamics revealed by molecular movie analysis (2021)
  • Author(s): Eriko Nango
  • Organizer: RIKEN BDR Symposium 2021 “Structuring Biosystems: FunctionsEmerging from Molecules”
  • Int'l Joint Research / Invited
• [Presentation] 「高速分子動画法のこれまでとこれから」 (2021)
  • Author(s): 南後 恵理子
  • Organizer: 2020年度 生物物理学会 北海道支部－東北支部 合同例会
  • Invited
• [Presentation] Photon Factory の散乱ビームラインの現状 (2021)
  • Author(s): 清水伸隆、高木秀彰、永谷康子、米澤健人、大田浩正、森丈晴、 谷田部景子、高橋正剛、西條慎也、鈴木文俊、羽方望、五十嵐教之
  • Organizer: 第34回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] ヘテロクロマチン蛋白質(HP1α)の溶液構造解析 (2021)
  • Author(s): 米澤健人、古川亜矢子、安達成彦、千田俊哉、清水伸隆、西村善文
  • Organizer: 第34回日本放射光学会年会・放射光科学合同シンポジウム
• [Presentation] 高速分子動画：タンパク質が機能する際の構造変化を可視化する (2020)
  • Author(s): 南後 恵理子
  • Organizer: 第43回日本分子生物学会年会シンポジウム 拡大する構造生物学 Expanding Structural Biology
  • Invited
• [Presentation] Protein dynamics structures revealed by time-resolved serial femtosecond crystallography (2020)
  • Author(s): Eriko Nango
  • Organizer: 第５８回日本生物物理学会年会シンポジウム
  • Invited
• [Presentation] Recent Progress of BioSAXS Equipment for Complex Molecular Systems at The Photon Factory (2020)
  • Author(s): Kento Yonezawa, Masatsuyo Takahashi, Keiko Yatabe, Yasuko Nagatani, Yugo Hayashi, Shinji Amano, Hironari Kamikubo, Nobutaka Shimizu
  • Organizer: 第20回日本タンパク質科学会年会
• [Presentation] Current Status of BioSAXS at the Photon Factory (2020)
  • Author(s): Nobutaka Shimizu, Kento Yonezawa, Masatsuyo Takahashi, Keiko Yatabe, Yasuko Nagatani
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] Recent hybrid methods approach utilizing Biological Small Angle X-ray Scattering at the Photon Factory (2020)
  • Author(s): Kento Yonezawa, Ayako Furukawa, Naruhiko Adachi, Toshiya Senda, Nobutaka Shimizu, Yoshifumi Nishimura
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] Molecular Dynamics Study of Multidrug Efflux Transporter AcrA-AcrB-AcrA-TolC Complex Embedded in Lipid Bilayer (2020)
  • Author(s): Keiko Shinoda
  • Organizer: 64th Annual Meeting of the Biophysical Society
• [Presentation] Time-resolved serial femtosecond crystallography at SACLA: Current status and future prospects (2020)
  • Author(s): Eriko Nango
  • Organizer: MicroMax User Meeting
  • Int'l Joint Research / Invited
• [Presentation] Hybrid Approach for Structural Biology: Simulation and Experimental Data (2019)
  • Author(s): 宮下治
  • Organizer: CBI 学会
  • Invited
• [Presentation] Catalytic Reaction Mechanisms Found by using the GLAS Algorithm (2019)
  • Author(s): Mitsuo Shoji
  • Organizer: ICPAC 2019
  • Int'l Joint Research / Invited
• [Presentation] 酵素の反応機構解明に適した構造探索手法(GLAS)の開発 (2019)
  • Author(s): 庄司光男
  • Organizer: 新学術領域研究「高速分子動画」キックオフミーティング・膜タンパ ク質研究会
• [Presentation] GLAS アルゴリズムによる酵素反応機構の新規解明 (2019)
  • Author(s): 庄司光男, 三嶋 謙二, 堀優太, 重田育照
  • Organizer: 第３３回分子シミュレーション討論会
• [Presentation] Reaction mechanism of water splitting in photosystem II revealed by QM/MM calculations (2019)
  • Author(s): Mitsuo Shoji
  • Organizer: 11th symposium on Discovery, Fusion, Creation of New Knowledge by Multidisciplinary Computational Sciences, CCS International Symposium 2019
  • Int'l Joint Research / Invited
• [Presentation] 多剤排出トランスポーター複合体AcrA-AcrB-AcrZ-TolCのMDシミュレーション (2019)
  • Author(s): 篠田恵子
  • Organizer: 第14回トランスポーター研究会年会（2019）
• [Presentation] 多剤排出トランスポーターAcrABZ-TolCの分子動力学シミュレーション：膜-膜タンパク質間相互作用の評価 (2019)
  • Author(s): 篠田恵子
  • Organizer: 第33回分子シミュレーション討論会
• [Presentation] SEC-SAXS による分子間相互作用測定解析システム (2019)
  • Author(s): 清水伸隆
  • Organizer: 第33回日本放射光学会年会・放射光科学合同シンポジウム
  • Invited
• [Presentation] BioSAXS experiment at the Photon Factory (2019)
  • Author(s): Shimizu, Nobutaka; Yonezawa, Kento; Takahashi, Masatuyo; Yatabe, Keiko, Nagatani, Yasuko
  • Organizer: International Symposium on Diffraction Structural BIology 2019
  • Int'l Joint Research
• [Presentation] Progress of Biological Small-Angle X-ray Scattering at the Photon Factory (2019)
  • Author(s): Nobutaka Shimizu, Kento Yonezawa, Masatsuyo Takahashi, Keiko Yatabe, Yasuko Nagatani
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] タンパク質が起こす反応を原子レベルで追跡する～X線自由電子レーザーによる分子動画撮影～ (2019)
  • Author(s): 南後恵理子
  • Organizer: 新規素材探索研究会
  • Invited
• [Presentation] A molecular movie of structural changes in the light-driven proton pump bacteriorhodopsin, (2019)
  • Author(s): Eriko Nango
  • Organizer: 2019 ESP-IUPB World Congress
  • Int'l Joint Research / Invited
• [Presentation] X線自由電子レーザーによるロドプシン類の分子動画解析 (2019)
  • Author(s): 南後恵理子
  • Organizer: ISSP ワークショップ「レチナールタンパク質の光機能発現の物理と化学
  • Invited
• [Presentation] X 線自由電子レーザーによるタンパク質分子動画撮影 (2019)
  • Author(s): 南後恵理子
  • Organizer: 第57回日本生物物理学会年会
  • Invited
• [Book] Gaussian16で始める量子化学超入門 (2019)
  • Author(s): 庄司光男
  • Total Pages: 70
  • Publisher: Amazon Kindle direct publishing (電子書籍)
• [Remarks] 高速分子動画領域ホームページ
  • URL: https://www.molmovies.med.kyoto-u.ac.jp/category/result/